Báo cáo khoa học: All-trans-retinoic acid inhibits collapsin response mediator protein-2 transcriptional activity during SH-SY5Y neuroblastoma cell differentiation pptx

... 2006 FEBS 511 All-trans-retinoic acid inhibits collapsin response mediator protein-2 transcriptional activity during SH-SY5Y neuroblastoma cell differentiation Lorena Fonta ´ n-Gaba ´ s 1 , Erik ... mediators is the protein known as collapsin response mediator protein-2, which interacts with and promotes tubulin polymerization. In this study, we investigated...

Ngày tải lên: 16/03/2014, 12:20

... [ 32 P]-tetrasodium pyrophosphate (Perkin-Elmer; specific activity in assay 5–10 cpmÆpmol )1 ), together with amino acid, tRNA and enzyme in 50 l L reac- tions. Radioactivity incorporated into ATP was quantified by ... [ 14 C]- amino acid, tRNA and arginyl-tRNA synthetase. Amino acid incorporation was followed using 3 MM filter discs (Whatman, Dassel, Germany) that had been pretreated with...

Ngày tải lên: 18/02/2014, 13:20

... decarboxylase activity below pH 6 as does the H298Q enzyme at all investigated pHs, whereas the wild-type enzyme retains a vestige of activity at pH 4 demonstrates that decarb- oxylase activity depends ... residue at position 328 is an important factor for efficient transacylation activity, especially at 4 °C. Elongation activity of the wild-type and K328 mutant KAS I proteins The tra...

Ngày tải lên: 19/02/2014, 08:20

... replicated three times. Fatty acid analysis Microalgae or yeast cells were harvested by centrifugation. Total fatty acids were extracted and transmethylated as previously described [14]. Fatty acid methyl esters ... dihydroxylated substrates. Abbreviations ARA, arachidonic acid; DHA, docosahexaenoic acid; EPA, eicosapentaenoic acid; EST, expressed sequence tag; FAME, fatty acid methy...

Ngày tải lên: 20/02/2014, 01:20

... ES-2 cells and cell growth activity was detected using a MTT assay. It was indicated that knockdown of NF-jB1 suppressed cell growth in a time-dependent manner. A significant reduc- tion in cell ... 0.05). MiR-9 inhibits ovarian cancer cell growth L M. Guo et al. 5540 FEBS Journal 276 (2009) 5537–5546 ª 2009 The Authors Journal compilation ª 2009 FEBS Knockdown of NF-jB1 inhibits...

Ngày tải lên: 07/03/2014, 00:20

... circumstances, amino acid availability regulates fatty acid homeostasis in the liver during the deprivation of an essential amino acid [6]. These examples demonstrate that Keywords amino acid; GCN2; gene ... expression in response to amino acid starvation. Results Amino acid starvation triggers changes in gene expression In order to identify amino acid- regulated genes, mouse...

Ngày tải lên: 07/03/2014, 03:20

... the food industries. Along with other weak acids such as sulfite and sulfur dioxide, sorbic acid, acetic acid, propionic acid and lactic acid, benzoic acid is used on a large scale as a food preservative, ... requirement of the cells upon sudden exposure to benzoic acid. The remaining question is why the cells need the energy. A possible answer is that cells need to maintain intracellu...

Ngày tải lên: 07/03/2014, 04:20

... chaperone activity with the wild-type protein. Each of the glutamic acid residue mutants displayed a decrease in chaperone activity compared with wild-type Hsp25. A. M. Morris et al. Glutamic acid ... comparable chaperone activity with the wild-type protein. Each of the glutamic acid residue mutants displayed a decrease in chaperone activity compared with wild-type Hsp25. Glutam...

Ngày tải lên: 07/03/2014, 04:20

... micellar detergents and fatty acids. The need for micellar structures explains why arachidic acid did not stabilize protofibrils in our study. The critical melting temperature for this fatty acid ... interaction of DHA with Ab have on Ab-induced cell toxicity. PC12 cells, a tumor cell line derived from rat adrenal gland, were chosen as a cell model, as these cells work very well with...

Ngày tải lên: 07/03/2014, 10:20

... IDH activity c ould be detected in the cell- free extract after ind uction with IPTG. The activity of the recombinant cell- extract (19.5 UÆmg )1 ) was 100-fold higher than that in the host cells ... recombinant E. co li. IDH activity was measured with carboxylation reaction. Step Total protein (mg) Total activity (U) Specific activity (UÆmg )1 ) Yield (%) Purification (fold) Cell-...

Ngày tải lên: 08/03/2014, 10:20