Báo cáo khoa học: "Joint Identification and Segmentation of Domain-Specific Dialogue Acts for Conversational Dialogue Systems" doc

... Linguistics Joint Identification and Segmentation of Domain-Specific Dialogue Acts for Conversational Dialogue Systems Fabrizio Morbini and Kenji Sagae Institute for Creative Technologies University of Southern ... 7.5 (with a maximum of 34, and minimum of 1), and the average length of utterances with multiple dialogue acts is 15.7 (max- imum of 66, min...

Ngày tải lên: 07/03/2014, 22:20

... regulation of specific herbicide- regulated gene expression. Results Identification of herbicide-regulated genes Two forward and reverse SSH libraries were made from pooled digestive glands and gills of ... and digestive gland of eight control and eight exposed oysters at days 0, 7, 15, 21 and 30 days using a method based on extraction in guani- dium isothiocyanate [80]. Fo...

Ngày tải lên: 30/03/2014, 15:20

... Tuovinen for the fruitful critical reading of the article. J.B. thanks Mark Dopson and Siv Sa ¨ a ¨ ffor practical help, Arunas Leipus for help in protein chromatography and Henrik Larsson for helpful ... production of p-nitrophenol was measured spectrophotometrically at 405 nm. One unit of the acid phosphatase activity was defined as the amount of protein which catalysed the...

Ngày tải lên: 07/03/2014, 14:20

... expression vector under the control of the T7 promoter, and coexpression of oraE and oraS genes was carried out in E. co li. Meanwhile, the extent of the involvement of AdoCbl or PLP in the in vivo folding process ... temperatures: 95 °C for 30 sec, 50 °C for 1 min, and 72 °Cfor4min. Finally, the reaction was maintained at 72 °Cfor5min. The PCR products were gel-purified, res...

Ngày tải lên: 30/03/2014, 15:20

... in ovary, testis and brain, the most significant expression of the WFIKKN gene is observed in adult pancreas, liver and thymus. In view of the presence of WAP-, Kazal-, Kunitz- and NTR-modules ... (Bubendorf, Switzerland). p-Nitrophenyl- p-guanidinobenzoate was a product of Fluka (Buch, Switzerland). Cloning and expression of the second Kunitz-type protease inhibitor module...

Ngày tải lên: 31/03/2014, 01:20

... of formation of these two states should be almost equal. The lifetime of each of the conformational states, and therefore the energy barrier between them, is ... were obtained for more than 99% of the 1 H, 13 C and 15 N atoms of the protein backbone, and for more than 78% of the side chain atoms. The main set of backbone u and w dihedral angles was calculated ... m...

Ngày tải lên: 06/03/2014, 11:20

... 25 °C. Extraction and preparation of mycelia for MALDI-TOF analysis A few micrograms of fungal mycelia were suspended in acetonitrile ⁄ methanol ⁄ water (1 : 1 : 1), and 1 lL of the sus- pension ... mass shifts of 8 Da of reduced and unreduced forms, the analysis of knockout mutants, and comparison with protein databases. Strain-speci- fic processing was observed in the c...

Ngày tải lên: 19/02/2014, 02:20

... for amplifications of 3¢-portions of Psrp-2 (P2-1/ PR) and Psrp-3 (P3-1/PR), and 50–57 °C for amplification of 3¢-portion of Psrp-4 (P4-1/PR). Here, for example, P2- 1/PR stands for PCR amplified ... to the 5¢ and 3¢ ends of cDNA inserts (see Materials and methods for further information), and both strands of the cDNAs obtained were completely sequenced. The nucle...

Ngày tải lên: 17/03/2014, 09:20

... of ether bonds with HI and subsequent reduction of the formed iodides was performed as described previ- ously [18]. Isolation of ladderane lipids For isolation of lipids the extract (54 mg) of ... University of Amsterdam, the Netherlands 3 Department of Microbiology, Institute of Water and Wetland Research, Radboud University Nijmegen, the Netherlands Recently, identific...

Ngày tải lên: 23/03/2014, 15:20

... lLof8 M urea and incubated at 37 °C for 1 h. To the solution, 60 lL of 0.2 M Tris/HCl buffer (pH 9.0) and 5 pmol of lysyl endopeptidase were added, and the m ixture was incubated at 37 °C for ... was dissolved in the standard buffer (pH 6.5) and dialyzed overnight against the same buffer. The enzyme s olution was kept at 60 °C for 30 min in the presence of 0.1 M D -asparta...

Ngày tải lên: 07/03/2014, 16:20