... wild-type and tRNA (lane 2,0.12 lM; lane 3, 0.3 lM; lane 4, 0.6 lM; lane 5, 1.2 lM, lane 6,2.4 lM); lanes 7–10, R347A and tRNA (lane 7, 0.12 lM; lane 8,0.3 lM; lane 9, 0.6 lM; lane 10, 1.2 ... flexibility of the methanogenic-type seryl-tRNAsynthetase active site and its implication for specificsubstrate recognitionSilvija Bilokapic1, Jasmina Rokov Plavec1, Nenad Ban2 and Ivana Weygand-Durasevic11 ... Kmvalues for serylation wereestimated for the expressed tRNAs and for in vitrotranscribed tRNACGAas a reference. The Km and kcatvalues for both isoacceptors were similar and compara-ble...