basic principles of planar chromatography and its potential for hyphenated techniques

... Thermostated Planar Chromatography 223 Paweł K Zarzycki Part IV HPTLC and its Future to Combinatorial Approach 14 Basic Principles of Planar Chromatography and Its Potential for Hyphenated ... much effort has been devoted to the coupling of high-performance thin-layer chromatography (HPTLC) with spectrometric methods It is because of the robustness and simplicity of use of HPTLC and the ... number of chiral selectors and chiral-stationary phases Notes HPTLC is a most versatile technique and is known for uniformity, purity profile, assay values and precision and accuracy of results...

Ngày tải lên: 06/10/2016, 09:26

... nm/band-pass 465-510 nm for CFP, 488 nm/bandpass 500-530 nm for GFP, 514 nm/ bandpass 525-555 nm for YFP, 561 nm/bandpass 575-630 nm for RFP and mCherry, and 488 nm/band-pass 650-710 nm for chloroplast ... information for identifying the relationship between flower-related genes and tandem repeat sequences Transient transformation is a suitable tool for determining the subcellular localization of ... Dr Yu-Yun Chang for helpful discussions and Dr Heiko Kuhn and Ms Miranda Loney for editing the manuscript This work was supported by the Development Program of Industrialization for Agricultural...

Ngày tải lên: 11/08/2014, 11:21

... and 450 nm Molecular mass information was obtained as described [6], determining the elution volumes of standard proteins and the void volume of the column The Superdex 200 PC 3.2/30 column and ... that Arg206, Asn210 and Ala276 are important for the second-half reaction and are part of the active-site of CoaB; these residues may be directly involved in binding of one of the substrates, ... removed and characterized by its UV spectrum (B, right figure) and MALDITOF mass spectrum (C) as 4¢-phosphopantothenoyl-CMP presence of cysteine methyl ester) Although the structures of PP and 4¢-phosphopantothenoylcysteine...

Ngày tải lên: 19/02/2014, 12:20

... Ribbon representation of the modelled SEA domain of human MUC1 The model consists of three a-helices and six b-strands forming an a ⁄ b sandwich fold The FRPG ⁄ SVVV residues of the MUC1 cleavage ... MUC3SEA and MUC1FDTR ⁄ MUC12SEA glycoproteins showed a major M2-reactive and CT-2 reactive noncleaved glycoform of about 105–120 kDa (Fig 4A,C, MUC3SEA and MUC12SEA) and a minor population of a ... cycles of 94 °C for 30 s, 60 °C for 30 s, 72 °C for with a final extension at 72 °C for The PCR generated fragments were digested with PsiI and DraIII and cloned into the respective sites of the...

Ngày tải lên: 19/02/2014, 18:20

... Their appetite for information and respect for their rights is clear To some extent, they are also aware of the unsatisfactory nature of delay and of cost and that the impact of these practical ... descriptions of their views and experiences in art form This data thus adds a welcome richness to this report and a special debt of gratitude is owed to the Ombudsman for Children’s Office in Ireland for ... the Rights of the Child (UNCRC) and other international standards At its meeting in December 2010, the Committee of Experts mandated to draft the guidelines agreed to consult children and young...

Ngày tải lên: 17/03/2014, 17:20

... ATF-2 and c-Jun can bind to p300 and CBP through multiple domains, with a preference of c-Jun for the N- and C-terminal regions and of ATF-2 for the central region of the coactivators Binding of ... and C2-regions of p300, and to the N- and C2-regions of CBP Thus, the bulk of the interaction between NF-jB and p300/CBP is mediated by the p65 subunit through the N- and C-terminal regions of ... Activity of the indicated combinations of transcription factors pairs (0.5 lg for ATF-2, c-Jun, IRF-3E7 and IRF-7Di; lg for IRF-1; 12 ng for p50 and 18 ng for p65) in the presence or absence of cotransfected...

Ngày tải lên: 23/03/2014, 13:20

... activity of this enzyme [10] Thus, Sec14p and its homologues appear to form a network of proteins involved in the regulation of phospholipid metabolism Materials and methods Strains and culture ... 2003 nucleotides upstream of the STOP codon of the ORF of interest and on the 3¢ end, 65–70 nucleotides of the genomic region downstream of the ORF of interest Transformants were recovered based ... secretion of GroPCho, overproduction of Sfh2p and Sfh4p significantly decreased the release of GroPCho into the medium As phospholipase B1 (Plb1p) is mainly responsible for the cleavage of PtdCho and...

Ngày tải lên: 23/03/2014, 18:20

... therapies for pancreas cancer and are an exciting class of compounds for potential treatment of these malignancies Methods Sigma receptor ligands Sigma-2 specific ligands SV119, SV95, and fluorescent ... split and preincubated for more than 24 hours (Panc-02) and 48 hours (CFPAC-1, AsPC-1, and Panc-1) to maintain their growth conditions SV119 and SW120 were dissolved in DMSO, and gemcitabine and ... achieving an early diagnosis and the failure of surgery, radiation and chemotherapy In fact, only 15% of patients are eligible for surgical resection at the time of diagnosis [3] Even after radical...

Ngày tải lên: 18/06/2014, 15:20

... subjects before death The DSM-IV axis I diagnosis of MDD and BD was established in consensus meetings of two psychiatrists (JS and HB) using all available information from interviews and clinical ... Illustrations of QUIN-immunoreactive cells from the left sACC of a depressed suicidal patient and a control case and the locations of the analyzed regions of interest (sACC, aMCC and pACC) Depressed ... this area could lead to a greater understanding of the pathophysiology of depressive disorders and pave the way for identification of novel biomarkers and therapeutic strategies targeting specific...

Ngày tải lên: 19/06/2014, 22:20

... etiology of autism and its commonly related psychiatric conditions Page of subjects enrolled in the study (25 autistic male patients and 16 age and gender matched controls) had written informed ... treated standard solution of K + chloride in Bovine albumin equivalent to mol/L Page of Table Caspase3, IL6, TNFa, Ca2+, Mg2+, Na+ and K+ concentrations and Ca2+/Mg2+ and Na+/K+ ratios in plasma of ... concentrations in the hair of autistic population and that of Krey and Dolmetsch [53] in which they proved that some forms of autism are caused by failures in activity-dependent regulation of neural development...

Ngày tải lên: 19/06/2014, 22:20

... discarded and the column was washed with 700 μl of buffer RW1 (supplied in kit) for 15 s at 20,000 rpm Two additional washes were performed with 500 μl of buffer RPE Hughes-Fulford and Li Journal of ... Sandra Spurlock for the data plate reading and data analysis of Table We would like to thank Tammy Chang for her thoughtful comments and suggestions during this work and Joe Meissler, Tara Candelario, ... mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc Fgf-2, on the other hand, significantly...

Ngày tải lên: 20/06/2014, 04:20

... discarded and the column was washed with 700 μl of buffer RW1 (supplied in kit) for 15 s at 20,000 rpm Two additional washes were performed with 500 μl of buffer RPE Hughes-Fulford and Li Journal of ... Sandra Spurlock for the data plate reading and data analysis of Table We would like to thank Tammy Chang for her thoughtful comments and suggestions during this work and Joe Meissler, Tara Candelario, ... mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc Fgf-2, on the other hand, significantly...

Ngày tải lên: 20/06/2014, 07:20

... between each band of the LRMS image and HR images to sharpen the former It models the relationship between the pixels of each band of the HRMS zb, the PAN image x and the corresponding band of the LRMS ... s PAN and s b are the standard deviation of the PAN and MS image band b, respectively This technique ensures that the mean and standard deviation of PAN image and MS bands are within the same ... characteristics of the PAN image match those of each band of the MS image or match those of a Page of 22 transformed image based on the MS image Unfortunately, this is not usually the case [16], and those...

Ngày tải lên: 20/06/2014, 22:20

... moisture levels of 20% (g·g–1) and 40% (g·g–1), whereas they were clearly separated at soil moisture of 60% (g·g–1) For a given elevation and its associated vegetation type, potential mineralizable ... temperature is maintained at 20°C For the given vegetation type of Changbai Mountain, soil C and N mineralization rate, potential mineralizable C (C0) and potential rate of initial C mineralization ... properties of the natural reserve on northern slope of Changbai Mountain Research of Forest Ecosystem, 2: 196–206 (in Chinese) Cole L., Buckland S.M., Bardgett R.D (2008): Influence of disturbance and...

Ngày tải lên: 07/08/2014, 10:21

... useful for further better theoretical understanding of the relation between measurable parameters of the heat field and sap flow and for methodical aspects of sap flow techniques MATERIALS AND METHODS ... Displacement of the hot spot out of its original position violates one of the main principles of the HFD method (constant power supply to the heater) and thus sap flow data calculated after switching off ... abrupt separation of the water source from its sink This simultaneously released the soil-root resistance and increased the gradient of water potential between leaves and the plane of cutting Also...

Ngày tải lên: 08/08/2014, 01:22

... petraea) is widely distributed in plain forests all over France and represents one of the major species used for timber production Even if lowland forests of western Europe are submitted to temperate ... moderate soil drought on the rate of CO assimilation and the amount of soluble and insoluble carbohydrates in leaves of 4-year-old saplings of Q petraea The aim of these experiments was to assess ... determinations were partly performed at the Université Nancy I, Laboratory of Forest Physiology, France The authors thank P Dizengremel and D Gérant for their help and for having provided laboratory...

Ngày tải lên: 08/08/2014, 18:21

... basis of genome size relative to a standard plant of known ploidy MATERIALS AND METHODS The plant material was leaves of in vitro cloned plantlets of Q petraea (Matt) Liebl, Q pubescens Willd and ... are the first for the genus Quercus and the Fagaera Therefore, family The values are typical for higher plants This type of data can be useful in planning molecular procedures for DNA polymorphism ... polymorphism analysis or the study of genetic architecture of genomes by RFLP and PFGE (choice of rarely cleaving restriction enzymes) or RAPD (choice of primers), and in speciation studies (Godelle...

Ngày tải lên: 08/08/2014, 18:21

... plants of Q robur, Q petraea and Q cerris, purchased in root trainers, were potted for use in 1992 and 1993 Two groups of 10 plants each, chosen from of the species, were droughted for periods of ... conditions A useful measure of drought susceptibility is, therefore, to define the relationship between loss of hydraulic conductivity and water potential (Tyree and Sperry, 1989) Unfortunately, this method ... using the method of Sperry et al (1988a) Each leaf was cut from its stem under degassed water and its petiole excised from the base of the leaf lamina before cutting to a length of 20 mm All operations...

Ngày tải lên: 08/08/2014, 19:21

... at the 40-year NE stand, the 70-year stand and the 110-year stand Predawn water potentials of small twigs of the trees at the 140-year, 40year, 40-year boggy and 40-year NE stand were measured ... 11.7 ± 1.0 mg g Therefore two ) -1 of the 40-year-old stands (40-year stand and 40-year boggy stand) and the three older stands were, concerning the nitrogen concentration of the 3-year-old needles, ... The Mg of the 40-concentrations 2+ year boggy stand and the 110-year stand are significantly different (P < 0.05) from the Mg of the 40-year-concentrations 2+ stand, the 40-year NE stand and the...

Ngày tải lên: 09/08/2014, 04:20

... Professor at Queen Mary and Westfield, UK Meghnad Desai is Professor of Economics and Director of the Centre for Study of Global Governance at the London School of Economics Sheila Dow is Professor ... the University of Ottawa Colin Rogers is Associate Professor and Dean of the School of Economics at the University of Adelaide, Australia Malcolm Sawyer is Professor of Economics and Leeds University ... Deputy Director of the Financial Markets Group and the Norman Sosnow Professor of Banking and Finance at the London School of Economics Augusto Graziani is Professor at the University of Rome, ‘La...

Ngày tải lên: 09/08/2014, 19:22