Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... number AI563351) was used to design four GAT -specific primers; CLGAT 5a (5¢-GGCATCAACAT- CACAAGCAACAAGTGCAAG-3¢), CLGAT5b (5¢-TCCT- CCATCAATCTGCTTCCATGGACCATC-3¢), CLGAT 3a (5¢-GCTGTGGCTACGAATGAGGCCGCC-3) ... FEBS Purification and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon Kentaro Takahara, Kinya Akashi and Akiho Yokota...

Ngày tải lên: 20/02/2014, 03:20

... Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii Maghil Denis, P. D. Mercy Palatty, N. Renuka Bai and S. ... increase in specific activity. Analysis of the lectin on SDS/PAGE gave a single band at apparent molecular mass of 34 kDa. The binding affinity of th...

Ngày tải lên: 21/02/2014, 00:20

... with an apparent molecular mass of 53 kDa appears as a double band in unboiled samples (lanes A1 and B1). Table 1. N-Terminal sequences of the polypeptides of the purified enzyme. N-Terminal sequen ... with g values at 2.031, 1.994, and 1.951. The resonance started to develop at potentials ‡ 0 mV and was stable at potentials up to +350 mV. The loss and formation of...

Ngày tải lên: 21/02/2014, 03:20

... M, Teramoto T, Kumagaye KY, Nakajima K, Watanabe T, Kawai T, Kawakami Y, Niidome T, Sawada K, Nishizawa Y et al. (1994) Omega-agatoxin- TK containing D-serine at position 46, but not syn- thetic ... China Sea. Sephadex G-25 was purchased from Amersham Biosciences (Uppsala, Sweden), a ZORBAX 300SB-C18 semipreparative column was from Agilent Tech- nologies (Santa Clara, CA, USA), and tr...

Ngày tải lên: 18/02/2014, 17:20

... CTGATCTAGAGGTACCGGATCC 5RACF ATCCTCACGAACAAGCAG 5RACR GATCGCGATGCAGGCCTT FLF1 GGACGACTACAGCGTCTTCAGTAGA FLR1 TCCAAACAGTCAGTTTCTTAACCGT Table 1. Purification of cellulase from abalone Haliotis discus hannai. ... of the cDNA library and cloning of cellulase cDNA was achieved as follows: Total RNA was extracted from 1 g of abalone hepatopancreas by the ganidinium thiocyanate-p...

Ngày tải lên: 20/02/2014, 23:20

... 3¢-RACE Zf3¢stat6-F2 CGGTAGTCAGGAAATCAATGCC 3¢-RACE Zf5¢stat6-R1 CCATGTCTGCAGATGGTCGAGG 5¢-RACE Zf5¢stat6-R2 GGACTGACATTGCTCCAGAGC 5¢-RACE Zf3¢stat6-F3 GCTTCAGTGACTCAGAAATTGG 3¢-RACE Zf3¢stat6-F4 GTCCAGAATATTCAGCCTTTCACC ... CTGGATTGAAGCGCCCTCGGTTAATC 3¢-RACE Zf5¢tbet-R1 GCTGCCTTTGTTATTTGTAAGCTTCAG 5¢-RACE Zf5¢tbet-R2 GGAAACTTCCTGTCTCATCCAGTG 5¢-RACE Zffoxp3-F1 GGAACACACAGAGGGGATGATA Initial...

Ngày tải lên: 16/02/2014, 09:20

... P (2000) The transcriptional co-activator P ⁄ CAF potenti- ates TGF-beta ⁄ Smad signaling. Nucleic Acids Res 28, 4291–4298. 39 Kahata K, Hayashi M, Asaka M, Hellman U, Kitagawa H, Yanagisawa J, Kato ... DeMarco R, Martins EA, Guimaraes PE, Ojopi EP, Paquola AC, Piazza JP, Nishiyama MY Jr, Kitajima JP, Adamson RE et al. (2003) Transcriptome analysis of the acoelomate human parasite Schis...

Ngày tải lên: 18/02/2014, 16:20

... GAGAATTCAAAAGAAAGCGGGGAAGAA Construction of pOpacWh W12 CGAGATCTTGTGGACATGGTCCCGTTTC Construction of pOpacWh S1 CGGAATTCGCTAATGGGAGGAAATCAC Construction of pOpacSh S2 TACAGATCTTTTCGTCTTGCGAATTTC ... of sipW W7 TTGTGTAAAAGTGATGACATCGCC Cloning of sipW W8 GTGATCCCGATTATTCTGTGTGTT Cloning of sipW W9 GGCGATGTCATCACTTTTACACAA Cloning of sipW W10 AACACACAGAATAATCGGGATCAC Cloning of...

Ngày tải lên: 21/02/2014, 03:20

... with a Thermal Cycler Dice Real Time System (TaKaRa Bio Inc.). Forward primers 5¢-CG TTTGAAGGGTGAGGAGGAAAA[FAM]G-3¢ and 5¢-CA CAAGAGAGTTCTGCGATAACCTTG[FAM]G-3¢ (Invi- trogen Corporation) and reverse ... reverse primers 5¢-AAGTAGGCA ACAAAACAACG-3¢ and 5¢-GTTTTCCCGACAATAA- CATGG-3¢ were used for detecting GmPDIL- 3a and GmPDIL-3b, respectively. Primers for quantification of actin mR...

Ngày tải lên: 18/02/2014, 11:20

... ON357, 5¢-TGAAACATCACCAACTAAATC TCCAA-3¢; ON358, 5¢-GACGACTCCATTGTTATAGG AAAAGAGT-3¢; ON359, 5 ¢-GCCGCTGGAGAAACAG CAT-3¢; ON360, 5¢-GCCACCCATTCGTCACAATC-3¢; and ON361, 5¢-ATGCAGAAGGGGATCCGG-3¢. Direct ... overexpression of Epac and mimicked by a cAMP analogue stimulating both PKA and Epac, but not by an Epac -specific cAMP analogue [25]. The differences in H89 sensitivity and po...

Ngày tải lên: 19/02/2014, 18:20