Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx

... investigated by studying the functional effects of deleting the motif in EF -Ts, both in vivo and in vitro. The motif was deleted in endogenous EF -Ts in E. coli strain UY211 by gene replacement, and the phenotype ... show the positions of the protruding coiled-coil motif (residues 187–226) and the C-terminal module. The terminal positions of...

Ngày tải lên: 21/02/2014, 00:20

... by xanthine oxidase was followed indirectly by measuring the increase in absorbance at 550 nm resulting from cyto- chrome c being reduced by superoxide in the course of the reaction. The principle of ... against superoxide. These proteins did not consume detectable amounts of O 2 when one of the following substrates was offered to a protein solution containing 7 lm metal...

Ngày tải lên: 18/02/2014, 06:20

... only critical for the stability of the dimer, it may also be involved in maintaining the integrity of the active site. These results clearly suggest that the dimer interface in the Y74W* mutant ... necessarily involves movement of proximal side chains. A cascade of side chain movements might then be expected to in uence the precise positioning of the functional...

Ngày tải lên: 18/02/2014, 11:20

... processed into buforin-I and fixed to the mucous surface of epithelial cells to prevent the entry of bacteria into the epithelial layer and their invasion into the lower parts of the gastro- intestinal ... 4). The bactericidal effects of H4-(86–100) and ciprofloxacin were completed within 30 min, as incubation of the cells for a longer period (2 h) in the presence...

Ngày tải lên: 18/02/2014, 14:20

... be the predominant transcript in the adult intestine and in the developing embryo, whereas Mxi1-SRb transcripts predominate in the adult liver and kidney [12]. In our initial description of Mxi1-SRa ... that the PRD may be involved in regu- lating other functional domains of Mxi1 (e.g. the SID or the bHLH ⁄ LZ) and ⁄ or in the recruitment of other activ- it...

Ngày tải lên: 18/02/2014, 16:20

... and the increase in the size of the side chain may create steric hindrance for the substrate, making the base moiety of the sub- strates bind in a nonoptimal conformation. Another interesting ... ion and arginines of the Lid-region sta- bilize the negative phosphates of this state. The most striking effect of mutation of arginine to histidine is an almost 200...

Ngày tải lên: 19/02/2014, 02:20

... to bind to the membrane of cells. Inhibitory effects of VVA1 on the hemolytic and cytotoxic activity of VVA2 The effects of VVA1 on the hemolytic activity of VVA2 were studied by incubation of ... ratio of 2) inhibited VVA2 binding to the cell membrane (Fig. 3, panels e–h). These results strongly suggest that VVA1 inhibits the cytotoxicity of VVA2 by preventing...

Ngày tải lên: 19/02/2014, 06:20

... region, including the PAS-B domain of BMAL1, is required for the binding to DEC1, Fig. 4. Identification of the domain of D EC1 that interacts with BMAL 1. Interactions of various DEC1 mutants with ... suppressive activity of DEC1 is also required for the interaction with BMAL1. Binding of DEC1 mutants to a CACGTG E-box To e xamine the binding ability o f DEC1 mutants to...

Ngày tải lên: 19/02/2014, 16:20

... Llinas M (1988) Lysi- ne ⁄ fibrin binding sites of kringles modeled after the structure of kringle 1 of prothrombin. Proteins 3, 85–96. 20 Thorsen S (1975) Differences in the binding to fibrin of native ... specifically inhibit the LBS function of either K1 [34] or K4 [35] and have used them to analyse, in functional isolation, the role of these LBS on plas- min(ogen) b...

Ngày tải lên: 20/02/2014, 01:20

... [14,20]. Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pairs [19], which is reminiscent of the structure ... important functional role, such as the correct positioning of the SAP motifs in relation to the rest of the protein as well as for the stabili...

Ngày tải lên: 20/02/2014, 11:20