Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

... crossover intermediate is therefore crucial for the integrity and maintenance of DNA in all organisms including mitochondrial DNA (mtDNA). Mitochondrial DNA amounts to about 15% of the DNA content in ... base-pairs in the centre of the junctions [19]. Detailed understanding of the functional role of these interactions would require the crystal str...

Ngày tải lên: 20/02/2014, 11:20

... mBmal1 cDNA as a template. The cDNAs thus obtained were subcloned into the mammalian two-hybrid vector pBIND (Promega) for expression of the GAL4 DNA- binding domain (DNA- BD) fusion protein. Luciferase ... C-terminal region, including the PAS-B domain of BMAL1, is required for the binding to DEC1, Fig. 4. Identification of the domain of D EC1 that interacts with BMAL 1. I...

Ngày tải lên: 19/02/2014, 16:20

... serine prote- ase located in the amino-terminal domain of NS3. Ser- ine protease activity of NS3 requires NS4A, a protein consisting of 54 amino acid residues, to form a stable complex with the ... factors, including IFN regulatory fac- tor-3 (IRF-3) and kinases, including the Tank-binding kinase 1 (TBK-1) and inhibitor of j B kinase e (IKK-e) [28–31]. Although IRF-3 is locate...

Ngày tải lên: 18/02/2014, 16:20

... by xanthine oxidase was followed indirectly by measuring the increase in absorbance at 550 nm resulting from cyto- chrome c being reduced by superoxide in the course of the reaction. The principle of ... catalysis, ligand binding, and the role of storage proteins, the cupins constitute one of the most diverse superfamilies of proteins known. They have been descri...

Ngày tải lên: 18/02/2014, 06:20

... in the size of the side chain may create steric hindrance for the substrate, making the base moiety of the sub- strates bind in a nonoptimal conformation. Another interesting feature concerning ... ion and arginines of the Lid-region sta- bilize the negative phosphates of this state. The most striking effect of mutation of arginine to histidine is an almost 200...

Ngày tải lên: 19/02/2014, 02:20

... Llinas M (1988) Lysi- ne ⁄ fibrin binding sites of kringles modeled after the structure of kringle 1 of prothrombin. Proteins 3, 85–96. 20 Thorsen S (1975) Differences in the binding to fibrin of native ... specifically inhibit the LBS function of either K1 [34] or K4 [35] and have used them to analyse, in functional isolation, the role of these LBS on plas- min(ogen)...

Ngày tải lên: 20/02/2014, 01:20

... show the positions of the protruding coiled-coil motif (residues 187–226) and the C-terminal module. The terminal positions of the deletion of the coiled-coil motif and the insertion of the linker ... rates in the presence of kirromycin The effect of kirromycin on the growth of GRd.tsf was investigated by measuring growth rates of cultures in LB conta...

Ngày tải lên: 21/02/2014, 00:20

... excluded all residues involved in the binding of the metal cofactor. ApeSODs in different forms were superimposed with the least square fit of Ca atoms of the residues in the range 10–200. ApeSOD ... focus on the coordination of the metal cofactor in the active site as well as the changes it experiences in response to different metal cofactors. Finally, by c...

Ngày tải lên: 14/02/2014, 22:20

... [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo assembly of the metallocofactor of C. ammoniagenes R2F is ... the increase of the concentration of the organic radical in response to added manganese indicates an obligatory role of this metal during in vivo...

Ngày tải lên: 15/02/2014, 01:20

... around the Thr-binding site is missing in TtAKb-free, and that b-strands surrounding the Thr-binding site show outward shifts in the absence of Thr, with 12° rotation of the ACT2 domain from the fixed ... stick model. Both ACT domains forming an effector-binding unit of the front of the dimer are indicated. Site 1 and site 2 of the effector-binding unit on the f...

Ngày tải lên: 18/02/2014, 08:20