Tài liệu Báo cáo khoa học: Fatty acid desaturases from the microalga Thalassiosira pseudonana pptx

... 273, 19055–19059. Microalgal front-end desaturases T. Tonon et al. 3412 FEBS Journal 272 (2005) 3401–3412 ª 2005 FEBS Fatty acid desaturases from the microalga Thalassiosira pseudonana Thierry ... marine microalgae for the production of oils and fatty acids, in particular the use of algal oils containing long chain polyunsaturated fatty acids (LCPUFAs). The most pr...

Ngày tải lên: 20/02/2014, 01:20

... would cause erroneous interpretation of the stereochemistry in the active site. The only significant differences, apart from the length of the fatty acids, in the active site between C163S–C12 and ... C163 residues in the asymmetric unit. The fatty acid residues were included in the model and refined. The structure is similar to the WT–C8 complex structure, but wit...

Ngày tải lên: 19/02/2014, 08:20

... supporting the existence of a protease that cleaves s-DAPK-1 protein in vivo. The reason why the cleavage band was not observed in this assay may be the rapid degradation of the purified protein from the cell ... band (Fig. 3B). These data suggest that the first two amino acids of the tail are critical for proteolytic susceptibility, and that the third and fourth amino acids...

Ngày tải lên: 18/02/2014, 17:20

... pH. Transcripts derived from the sequences of the tRNA Arg ACG genes from jack bean and soybean were arginylated to only 6–10% of the theoretical acceptance by the arginyl-tRNA synthetases from both jack ... by canavanylation of the jack bean tRNA transcript using arginyl-tRNA synthetase from soybean. The stability of the isolated charged tRNA was compared in the prese...

Ngày tải lên: 18/02/2014, 13:20

... thetase to esterify the 13cROL generated in the reac- tion. Therefore, 13cROL is accumulated in the absence of LRAT in the reaction (Fig. S3B). Under the in vitro assay conditions of the ... the entire thickness of the inner retina. The Mu ¨ ller cell constitutes an ana- tomical link between the retinal neurones and supports their activities by exchanging molecules between...

Ngày tải lên: 14/02/2014, 14:20

... mitochondria during apoptosis. They induce the permeabilization of the mitochondrial membrane with the release of cytochrome c and the formation of the apoptosome [38]. The major pro-apoptotic protein Bax ... mitochondria and then translocate to the nucleus where they induce DNA fragmentation in a caspase-independent manner. Whether apoptosis is induced or not depends on the a...

Ngày tải lên: 14/02/2014, 22:20

... enthalpy is the sum of two terms: the reaction enthalpy, independent of the buffer used in the experiment, and another term representing the contribution of the proton ionization of the buffer, which ... HCl, 1 mM MgCl 2 , 100 mM NaCl. The upper panels show the raw data for the heat effect during the titrations; the lower panels are the binding isotherms. Table 2. Th...

Ngày tải lên: 16/02/2014, 14:20

... 1D). To further define the Meis2d transcriptional AD, we tested two other GBD fusion proteins, which contained either the Meis2 HD and C-terminal region, or just the region C-terminal to the HD. ... for the inhibi- tory effect of the Hth domain, we created a series of GDB fusion proteins (Fig. 4F). Deletion of either the N-terminal 65 or the N-terminal 97 amino acids did not de...

Ngày tải lên: 16/02/2014, 15:20

... allowing the user to select from a list of reactions. The user can then specify the direction of the reaction, and which substrate concentration was varied during the assays. Enzyme kinetics: from ... cap- tures the enzyme concentration used in the assay, allowing the kinetic parameter to be submitted as a k cat value. This decouples the parameter from the enzyme con...

Ngày tải lên: 18/02/2014, 04:20

... A from the com- plex with cathepsin H are shown in cyan (PDB code: 1NB3) [28]. The two structures of stefin A from the complex with cathepsin B are shown in red. The stefin B structure from the ... residues, A49 from the tip of the first binding loop and L73 from the second binding loop, fill the places that the two histi- dines occupy in the native structure. Besi...

Ngày tải lên: 18/02/2014, 04:20