Tài liệu Báo cáo khoa học: Differentiation stage-dependent preferred uptake of basolateral (systemic) glutamine into Caco-2 cells results in its accumulation in proteins with a role in cell–cell interaction pptx

... FEBS Glutamine incorporation in Caco-2 proteins K. Lenaerts et al. Differentiation stage-dependent preferred uptake of basolateral (systemic) glutamine into Caco-2 cells results in its accumulation ... intestinal injury [35], few data are available onthe uptake mechanisms of glutamine by the basolateral membrane of Caco-2 cells. As mentioned above, sys-tem L plays a role in glutamine uptake across ... below.Measurement of L -glutamine uptake by Caco-2 cells Glutamine uptake in Caco-2 cells was initiated by addingexperimental medium containing 0.1, 2.0 or 8.0 mml-gluta-mine, trace-labelled with...

Tài liệu Báo cáo khoa học: Afﬁnity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx

... plot of thebinding-inhibition curve was curvilinear, indicative of high-afﬁnity andlow-afﬁnity sites for PCSK9 binding on HepG2 cells. Nonlinear regressionanalysis of the binding data also indicated ... triplicate wells were incubated with 0.5 mL of incubation medium containing 10 lgÆmL)1 of antibodyagainst LDLR for 90 min at 4 °C. The cells were thenwashed, and the amount of bound antibody against ... SR, TaylorCA Jr & Attie AD (1983) A radioiodinated, intracellu-larly trapped ligand for determining the sites of plasmaprotein degradation in vivo. Biochem J 212, 791–800.46 Martinsson...

Tài liệu Báo cáo khoa học: The tandemly repeated domains of a b-propeller phytase act synergistically to increase catalytic efﬁciency doc

... InsP6gradually into InsP5, InsP4,and the ﬁnal product – Ins(2,4,6)P3and Ins(1,3,5)P3–via two alternative pathways [14].Bacterial BPPs containing two tandemly repeateddomains (dual domains) ... wasdetermined using the Bradford assay with BSA as thestandard [28].Phytase activity assayPhytase activity was determined by measuring the amount of phosphate released from InsP6using a modiﬁed ... can increasethe amount of available phosphate by interacting together. Additionally,fusing PhyH-DI to a single-domain phytase appears to be an efﬁcient wayto improve the activity of the latter.IntroductionPhytate...

Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt

... development and use of N-acylsulfonamides and sulfonimides as antagonists of nucleic acid-binding proteins. DatabaseStructural data for the two RNase A complexes are available in the Protein Data Bank ... Functional and structural analyses of N-acylsulfonamide-linked dinucleoside inhibitors of RNase A Nethaji Thiyagarajan1, Bryan D. Smith2,*, Ronald T. Raines2,3and K. Ravi Acharya11 Department ... USAIntroductionUpon catalyzing the cleavage of RNA, RNases operateat the crossroads of transcription and translation.Bovine pancreatic RNase A (EC 3.1.27.5) is the bestcharacterized RNase. A notoriously stable...

Tài liệu Báo cáo khoa học: Active and regulatory sites of cytosolic 5¢-nucleotidase doc

... GCATCAACTTTCAAAAGATF127E CGAGATAAGGGGACCAG TTAAATCCATGTGCACAGR144E AGAAGATGACACTGAAAG TGAATAAATTTATTTGGATACI152D CGATCTGAACACACTATTC TAAAATCTTTCAGTGTCATN154D GGACACACTATTCAACCT AGAATGTAAAATCTTTCAGTK31 1A ... AAGAAAGTAACTGACGACATGGACATGTG CACATGTCCATGTCGTCAGTTACTTTCTTY55G AGTGTTTTGGGTTTGACATGGATGGCACACTTGCTG CAGCAAGTGTGCCATCCATGTCAAACCCAAAACACTT56V AGTGTTTTGGGTTTGACATGGATTATGTGCTTGCTG CAGCAAGCACATAATCCATGTCAAACCCAAAACACTRegulation ... AGAATGTAAAATCTTTCAGTK31 1A CGCGCTGAAAATTGGTAC CCAGTTTTAGTATCCACCG319D GGACCCCTTACAGCA GTGTAGGTACCAATTTTCD39 6A GGCTATTTTCTTGGCTGA AAGCTCTGAAGCTCTTCM436W GTGGATGGGGAGCCTG CCGTAGCACATGTCCAH428D AAGAAAGTAACTGACGACATGGACATGTG...

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... Canyuk B, Focia PJ & Eakin AE (2001) The role foran invariant aspartic acid in hypoxanthine phospho-ribosyltransferases is examined using saturation muta-genesis, functional analysis, and ... hypoxanthinebases, respectively, indicating that Asp137 functions as a catalytic base [12]. However, a tight binding of N7 of the purine base and Asp137 was observed in HPRT in complex with ImmGP, indicating ... ImmGP, indicating a role in transitionstate stabilization during catalysis [17]. A series of mutations were made of the invariantAsp in HPRT from Trypanosoma cruzi. The D137Gmutation in this enzyme,...

Tài liệu Báo cáo khoa học: A strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics ppt

... quantitative interaction data in terms of the afﬁnity constants (K a ) of a series of lectins for a panel of pyridylaminated glycansobtained by automated frontal afﬁnity chromatogra-phy with ... compared with those of RCA120. Overlapping proteins appearing in the dark gray area of the Venn diagram are thought to be relatively abundant in serum,and are primary candidates for further validation. ... a common glycome, arguing for a commonorigin. Nat Chem Biol 5, 244–250.16 Kuno A, Itakura Y, Toyoda M, Takahashi Y, YamadaM, Umezawa A & Hirabayashi J (2008) Development of a data-mining...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... CTGGATTGAAGCGCCCTCGGTTAATC 3¢-RACEZf5¢tbet-R1 GCTGCCTTTGTTATTTGTAAGCTTCAG 5¢-RACEZf5¢tbet-R2 GGAAACTTCCTGTCTCATCCAGTG 5¢-RACEZffoxp3-F1 GGAACACACAGAGGGGATGATA Initial PCRZffoxp3-R1 CTTCAACACGCACAAAGCAC Initial ... GTCCAGAATATTCAGCCTTTCACC 3¢-RACEZftbet-F1 CTCCCTCAAACAAACCAGAGTC Initial PCRZftbet-R1 CACTGGATGAGACAGGAAGTT Initial PCRZf3¢tbet-F1 CTTCTCCAGGACAGTCCAAAGAGTC 3¢-RACEZf3¢tbet-F2 CTGGATTGAAGCGCCCTCGGTTAATC ... 3¢-RACEZf3¢stat6-F2 CGGTAGTCAGGAAATCAATGCC 3¢-RACEZf5¢stat6-R1 CCATGTCTGCAGATGGTCGAGG 5¢-RACEZf5¢stat6-R2 GGACTGACATTGCTCCAGAGC 5¢-RACEZf3¢stat6-F3 GCTTCAGTGACTCAGAAATTGG 3¢-RACEZf3¢stat6-F4 GTCCAGAATATTCAGCCTTTCACC...

Tài liệu Báo cáo khoa học: Post-translational modiﬁcation of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx

... ﬂuorescence staining technique that enablesvisualization of extracellular and intracellular bacteria in the same cell [25]. The results concurred with thedata from the gentamicin-based invasion assay. ... reveal a new aspect of thecomplex interplay in host–pathogen interactions anddemonstrate a physiological role of the deubiquitinat-ing enzyme OTUB1 in Yersinia invasion. OTUB1 as a potential ... subsequently inhibitsGaq-mediated signalling pathways including RhoAactivation and cytoskeletal rearrangements in the hostcell [15]. In addition, a crystallography-based studyrevealed that YpkA mimics...

Tài liệu Báo cáo khoa học: The Saccharomyces cerevisiae vacuolar acid trehalase is targeted at the cell surface for its physiological function docx

... TAGAATGGCTTTTGAAAAAAATAAAAAAGACAATAAGTTTTATAACCTTCAAATTGCTTTGGCSGA1_D CAGAGAAACAAGCAAAACAAAAAGCTTTTCTTTTCACTAACGTATATGATGGCAAGACAAAAGATGTTSGA1_R TAGAATGGCTTTTGAAAAAAATAAAAAAGACAATAAGTTTTATAACCTCTACAAACTCTGTAAAACTTATH1_suc2 ... GGGACGTCATACGGATAGCCCGCATAGTCAGGAACATCGTATGGGTACATGGGTTTTTTCTCCTTGACGR1_pLC1 TTTAGAATGGCTTTTGAAAAAAATAAAAAAGACAATAAGTTTTATAACCTGAATTCGAGCTCGTTTAAACPEP4_D CAGAGAAACAAGCAAAACAAAAAGCTTTTCTTTTCACTAACGTATATGATGTTCAGCTTGAAAGCPEP4_R TAGAATGGCTTTTGAAAAAAATAAAAAAGACAATAAGTTTTATAACCTTCAAATTGCTTTGGCSGA1_D ... ATGATGATGATAACAAAGGAGCTACAATCAAGGAAATTGTTCTCAATGATCGGATCCCCGGGTTAATTAAR1_ATH1 ATCCAAACTTATAATATTAAAAAAAGCGCTACTTATATGCATCATTTCATGAATTCGAGCTCGTTTAAACATH1_pUG36_D GCACTAGTATGAAAAGAATAAGATCGCTTTATH1_pUG36_R...

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