Tài liệu Báo cáo khoa học: Differentiation stage-dependent preferred uptake of basolateral (systemic) glutamine into Caco-2 cells results in its accumulation in proteins with a role in cell–cell interaction pptx

... FEBS Glutamine incorporation in Caco-2 proteins K. Lenaerts et al. Differentiation stage-dependent preferred uptake of basolateral (systemic) glutamine into Caco-2 cells results in its accumulation ... intestinal injury [35], few data are available on the uptake mechanisms of glutamine by the basolateral membrane of Caco-2 cells. As mention...

Ngày tải lên: 20/02/2014, 01:20

... plot of the binding-inhibition curve was curvilinear, indicative of high-affinity and low-affinity sites for PCSK9 binding on HepG2 cells. Nonlinear regression analysis of the binding data also indicated ... triplicate wells were incubated with 0.5 mL of incubation medium containing 10 lgÆmL )1 of antibody against LDLR for 90 min at 4 °C. The cells were then washed, and the amoun...

Ngày tải lên: 14/02/2014, 14:20

... InsP 6 gradually into InsP 5 , InsP 4 , and the final product – Ins(2,4,6)P 3 and Ins(1,3,5)P 3 – via two alternative pathways [14]. Bacterial BPPs containing two tandemly repeated domains (dual domains) ... was determined using the Bradford assay with BSA as the standard [28]. Phytase activity assay Phytase activity was determined by measuring the amount of phosphate released from InsP...

Ngày tải lên: 14/02/2014, 15:20

... development and use of N-acylsulfonamides and sulfonimides as antagonists of nucleic acid-binding proteins. Database Structural data for the two RNase A complexes are available in the Protein Data Bank ... Functional and structural analyses of N-acylsulfonamide- linked dinucleoside inhibitors of RNase A Nethaji Thiyagarajan 1 , Bryan D. Smith 2, *, Ronald T. Raines 2,3 and K....

Ngày tải lên: 14/02/2014, 22:20

... GCATCAACTTTCAAAAGAT F127E CGAGATAAGGGGACCAG TTAAATCCATGTGCACAG R144E AGAAGATGACACTGAAAG TGAATAAATTTATTTGGATAC I152D CGATCTGAACACACTATTC TAAAATCTTTCAGTGTCAT N154D GGACACACTATTCAACCT AGAATGTAAAATCTTTCAGT K31 1A ... AAGAAAGTAACTGACGACATGGACATGTG CACATGTCCATGTCGTCAGTTACTTTCTT Y55G AGTGTTTTGGGTTTGACATGGATGGCACACTTGCTG CAGCAAGTGTGCCATCCATGTCAAACCCAAAACACT T56V AGTGTTTTGGGTTTGACATGGATTATGTGCTTGCTG...

Ngày tải lên: 15/02/2014, 01:20

... Canyuk B, Focia PJ & Eakin AE (2001) The role for an invariant aspartic acid in hypoxanthine phospho- ribosyltransferases is examined using saturation muta- genesis, functional analysis, and ... hypoxanthine bases, respectively, indicating that Asp137 functions as a catalytic base [12]. However, a tight binding of N7 of the purine base and Asp137 was observed in HPRT in co...

Ngày tải lên: 15/02/2014, 01:20

... quantitative interaction data in terms of the affinity constants (K a ) of a series of lectins for a panel of pyridylaminated glycans obtained by automated frontal affinity chromatogra- phy with ... compared with those of RCA120. Overlapping proteins appearing in the dark gray area of the Venn diagram are thought to be relatively abundant in serum, and are primar...

Ngày tải lên: 16/02/2014, 08:20

... CTGGATTGAAGCGCCCTCGGTTAATC 3¢-RACE Zf5¢tbet-R1 GCTGCCTTTGTTATTTGTAAGCTTCAG 5¢-RACE Zf5¢tbet-R2 GGAAACTTCCTGTCTCATCCAGTG 5¢-RACE Zffoxp3-F1 GGAACACACAGAGGGGATGATA Initial PCR Zffoxp3-R1 CTTCAACACGCACAAAGCAC Initial ... GTCCAGAATATTCAGCCTTTCACC 3¢-RACE Zftbet-F1 CTCCCTCAAACAAACCAGAGTC Initial PCR Zftbet-R1 CACTGGATGAGACAGGAAGTT Initial PCR Zf3¢tbet-F1 CTTCTCCAGGACAGTCCAAAGAGTC 3¢-RACE Zf3¢ tbet...

Ngày tải lên: 16/02/2014, 09:20

... fluorescence staining technique that enables visualization of extracellular and intracellular bacteria in the same cell [25]. The results concurred with the data from the gentamicin-based invasion assay. ... reveal a new aspect of the complex interplay in host–pathogen interactions and demonstrate a physiological role of the deubiquitinat- ing enzyme OTUB1 in Yersinia inv...

Ngày tải lên: 16/02/2014, 15:20

... TAGAATGGCTTTTGAAAAAAATAAAAAAGACAATAAGTTTTATAACCTTCAAATTGCTTTGGC SGA1_D CAGAGAAACAAGCAAAACAAAAAGCTTTTCTTTTCACTAACGTATATGATGGCAAGACAAAAGATGTT SGA1_R TAGAATGGCTTTTGAAAAAAATAAAAAAGACAATAAGTTTTATAACCTCTACAAACTCTGTAAAACTT ATH1_suc2 ... GGGACGTCATACGGATAGCCCGCATAGTCAGGAACATCGTATGGGTACATGGGTTTTTTCTCCTTGACG R1_pLC1 TTTAGAATGGCTTTTGAAAAAAATAAAAAAGACAATAAGTTTTATAACCTGAATTCGAGCTCGTTTAAAC PEP4_D CAGAGAAAC...

Ngày tải lên: 18/02/2014, 06:20