Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx

... Ó FEBS 2004 Complex transcriptional and translational regulation of iPLA 2 c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization David ... presence of dual- competing subcellular localization s ignals in discrete isoforms of iPLA 2 c ;and (g) demonstrate t he functional integrity...

Ngày tải lên: 19/02/2014, 16:20

... occur for Ile38 in the hydrophobic core or for residues close to this core (i.e. Lys21 and Gln22, neighboring Phe20; Tyr25, neighboring Leu26), as well as for His52 (Fig. 1), because of slow conformational ... are obtained for N-terminal and C-terminal residues, for the loop, and partially for helix III, pointing to large- amplitude motions. Higher generalized order pa...

Ngày tải lên: 18/02/2014, 16:20

... Cys-free conotoxin and originated from C. marmoreus. D-Phe13 in conomarphin In order to obtain more material for structural studies, conomarphin was chemically synthesized using stan- dard Fmoc–l-amino acids. ... superimposed structures of conomarphin (red) and L-Phe13-conomarphin (gray). The side chain of L-Phe13 might cause spatial hindrance to the side chain of Lys9 and H...

Ngày tải lên: 18/02/2014, 17:20

... 2–6 for trappin-2, lane 8 for elafin). (B) Effect of class-specific protease inhibitors pepstatin (Peps., inhibitor of aspartyl proteases), AEBSF (inhibitor of serine proteases), E64 (inhibitor of ... activity of trappin-2 is independent of its intrinsic inhibitory activity and that trappin-2 and its uninhibitory mutant are bactericidal because fewer surviving CFU were prese...

Ngày tải lên: 18/02/2014, 17:20

... 51–55. Supporting information The following supporting information is available: Fig. S1. The in uence of (A) divalent cations, (B) pH and (C) temperature on Rep245 polymerase activity. This supporting ... supporting information can be found in the online version of this article. Please note: Blackwell Publishing are not responsible for the content or functionality of any suppo...

Ngày tải lên: 18/02/2014, 18:20

... developing therapeutic leads for treating disorders associated with increased levels of quinolinate and ⁄ or picolinate. Experimental procedures PCR amplification and cloning of ACMSD isoforms Human ... restored the bacterial protein [21,33]. Interestingly, the same difficulty in stripping off the metallocofactor from the protein has been described for murine adenosine deaminase...

Ngày tải lên: 19/02/2014, 02:20

... responsible for binding substrate and NADP cofactor. The detailed structural information might expedite the discovery of novel SDH inhibitors and further of antimicrobial agents, though few SDH inhibitors ... growth of H. pylori with MIC of 16, 16, and 32 lgÆmL )1 , respectively. This current work is expected to favor better understanding the features of SDH and provide us...

Ngày tải lên: 19/02/2014, 05:20

... and autophosphorylates. The generated phosphotyrosine sites constitute binding sites for a variety of down- stream proteins with SH2 domains. In order to deter- mine the signaling pathways resulting in ... cytoplasmic and nuclear isoforms, which originate from different translational start sites. Nucleocytoplasmic shuttling has been shown for both cytoplasmic forms of...

Ngày tải lên: 19/02/2014, 07:20

... of penicillamine and cysteine to form a disulfide bond will increase the number of amino acids in the peptide. The information obtained from truncating the peptides will also be useful in the design of ... interaction. Thus, in terms of inhibition of cell adhesion, cVY has more potential since it may directly interrupt the binding mech- anism of CD2–CD58. Inhibition of ce...

Ngày tải lên: 19/02/2014, 13:20

... cm) and Sephadex G -15 columns (1.6 · 80 cm) in pyridinium-acetate buffer, pH 4.5 (4 mL of pyridine and 10 mL of AcOH in 1 L of water), and the eluate was monitored by a refractive index detector. Configuration ... (zymosan a nd heat-killed Staph. aureus for TLR2; PolyIC for TLR3; E. coli Re595 LPS for TLR4; R848 for TLR7; and CpG O ligo ( 2 l M ) for TLR9), and t...

Ngày tải lên: 19/02/2014, 16:20