... accumulation of oxidative stress in various ubiquinone-deficient strains and in the cyc1 mutant by measuringmRNA levels of stress-inducible genes and the phosphorylation level of theSpc1 MAP kinase. Induction ... Eugene,OR, USA). Cells were suspended in 10 mm Hepes (pH 7.4)containing 5% glucose and MitoTracker Red FM at a finalconcentration of 100 nm. After 15 min of incubation at roomtemperature, cells ... Induction of ctt1, encoding catalase, and apt1, encodinga 25 kDa protein, but not that of gpx1, encoding glutathione peroxidase,was indistinguishable in four ubiquinone-deficient mutants, indicating...