Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... presence of full-length DEC1 As DEC1 lacking the basic region interfered with full-length DEC1 for the transcriptional suppression in the absence of CLOCK/BMAL1 [9], we examined whether DEC1Dbasic ... for DEC1 suppressive activity against CLOCK/BMAL1-induced transcription. In addition, the N-terminal region of DEC1, including the bHLH domain, interacted with t...

Ngày tải lên: 19/02/2014, 16:20

... FEBS 4773 of Q A – in PSII centers that are affected by the nonsatu- rating concentration of inhibitor [7,8]. The E m of Q A is raised in the presence of DCMU in the Q B pocket, making it energetically ... to the reduction of the PQ pool. Results As reported in the literature, the I step of the O–J–I–P fluorescence transient cannot be clearly distinguished...

Ngày tải lên: 19/02/2014, 05:20

... [14,20]. Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pairs [19], which is reminiscent of the structure ... removal of the N-terminal helices 1 and 2 while leaving the interacting a-helix 8 intact, may destabilize the proteins leading to spurious protein–prote...

Ngày tải lên: 20/02/2014, 11:20

... certain Trp residues in the C-terminus of HpPex5p to the dansyl cap in the PTS1 peptides, the distance between the dansyl cap in L2 and the three tryptophan residues in the model were measured. The values ... structure of the N-terminal domain of the human homologue, solved at 2.2 A ˚ resolution [11]. The obtained model covers residues 254–568 of HpPex5p,...

Ngày tải lên: 21/02/2014, 00:20

... simi- larly docked into the saccharide-binding site of Calsepa (RCSB Protein Data Bank code 1OUW) [28]. Cartoons showing the docking of Man ⁄ MeMan in the mannose-bind- ing sites of the lectins were drawn ... the N-ter- minus as well as the histidine tag at the C-terminus of the recombinant lectin apparently do not in uence the biological activity of Orysata, si...

Ngày tải lên: 14/03/2014, 23:20

... Most likely, the main players in deter- mining the sensitivity of dopaminergic neurons are altered dopamine homeostasis and a-synuclein misre- gulation. The analysis reported in the present study highlights ... proteins and allows us to enrich functional categories associated with the combination of factors analyzed in the present study (i.e. dopamine together with a...

Ngày tải lên: 15/02/2014, 01:20

... by xanthine oxidase was followed indirectly by measuring the increase in absorbance at 550 nm resulting from cyto- chrome c being reduced by superoxide in the course of the reaction. The principle of ... Interestingly, the position of the glutamate ligand was not conserved in the amino acid sequence, relative to the cupin core motifs that contribute the three his...

Ngày tải lên: 18/02/2014, 06:20

... o-NAC. The actual binding site of serotonin cannot be inferred from these results. Although serotonin can bind to the active site binding locus (W82), binding to PAS cannot be ruled out. If serotonin ... matching the observations obtained (Table 1). The binding of tyramine to D70G, in the presence of o-NPA, is weaker (9.1-fold) than binding to the wild-type enzyme, and ind...

Ngày tải lên: 18/02/2014, 17:20

... saturating in our experiments, when evaluating the impact of the muta- tions from the kinetic data, a change in the K m value can be interpreted as an effect on substrate binding, and a change in ... bromovinyl group may interact with the amino acids lining this space. Binding of BVDU to Dm-dNK-Y70W compen- sates for the loss of one hydrogen bond to 3¢-OH by a tighter...

Ngày tải lên: 19/02/2014, 02:20

... Waals ’ interactions and stacking of aromatic side chains with the porphine core of the porphyrins, as observed in the jacalin–H 2 TPPS interaction, most likely favor the binding of porphyrins to ... that binding of the same porphyrin to jacalin is mediated by a combination of hydrogen bonding and nonpolar inter- actions, including aromatic stacking interactions between...

Ngày tải lên: 19/02/2014, 16:20