Tài liệu Báo cáo khoa học: Receptor association and tyrosine phosphorylation of S6 kinases pdf

... involvement of src and PDGFR in tyrosine phosphorylation of S6K further, we studied the effect of inhibitors on tyrosine phosphorylation of S6Ks. As expected, genistein, a broad-range tyrosine kinase ... (A) Deletion of the N-terminus leads to a loss of phos- photyrosine in S6K. Hek293 cells were transiently transfected with WT and truncated mutants of S6K (S6K1, S...

Ngày tải lên: 19/02/2014, 07:20

... dis- tribution of the energy between the overall tumbling and the internal mobility, and is indicative of the degree of internal restraint of each 15 N– 1 H vector motion. In Fig. 4, most of the points ... graph of R 1 , R 2 and { 1 H}– 15 N NOE val- ues along the sequence of the TTF1 HD at 11.7 T and 286 K. Fig. S2. Plot of J(0.87x N )–J(0) correlation from 15 N relax...

Ngày tải lên: 18/02/2014, 16:20

... Pro, Val or Lys, c-carboxylation of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues. It ... structure of conomarphin was similar to those of other conotoxin cDNAs. The cDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 re...

Ngày tải lên: 18/02/2014, 17:20

... and the CFU counted. The per- centage of surviving CFU was calculated by the formula N ⁄ N control · 100, where N and N control were the numbers of CFU obtained after 3 h of incubation with and ... (2006) Secretion of serine peptidase by a clinical strain of Candida albicans: influence of growth conditions and cleavage of human serum proteins and extracellular matrix co...

Ngày tải lên: 18/02/2014, 17:20

... concentration of Rep245 pre- pared in 10 mm sodium phosphate (pH 7.4) was 5 lm. CD spectra were recorded at 60, 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of 1 ... presence of 0.2 mm dNTPs and 0.5 U of Taq DNA polymerase (Promega). To analyze the effect of temperature on primase activity, Rep245 and Rep516 proteins were incubated...

Ngày tải lên: 18/02/2014, 18:20

... software swiss-pdbviewer and visual molecular dynamics [49]. Acknowledgements We thank G. Magni and S. Ruggieri for helpful com- ments and suggestions and T. Begley (Department of Chemistry and ... electrophoresis of the PCR product indicated the presence of two distinct bands between 1000 and 1100 bp of roughly the same extent (not shown). Clo- ning and nucleotide seque...

Ngày tải lên: 19/02/2014, 02:20

... catalytic proper- ties of HpSDH and the effects of pH and temperature on HpSDH. The results show that HpSDH has a k cat of 7.7 ± 0.9 s )1 , K m of 0.148 ± 0.028 mm and k cat ⁄ K m of 5.2 · 10 4 m )1 Æs )1 toward ... shikimate, k cat of 7.1 s )1 and K m of 0.182 mm toward NADP, k cat of 5.2 s )1 and K m of 2.9 mm toward NAD. The optimum pH of the enzyme activity i...

Ngày tải lên: 19/02/2014, 05:20

... bonds between NH of Thr5 and CO of Arg2, as well as NH of Arg2 and CO of Thr5. Structure of cyclic hexapeptides – cYT peptide. The NMR data of the cYT peptide wer e indicative of its flexible nature. ... structure of peptide cER consists of two b-turns, located at the N- and C-termini. A comparison of the b-turn structure of cER with the similar region in the crysta...

Ngày tải lên: 19/02/2014, 13:20

... Fragments of HSQC and HMBC spectra of compound 1. Labels illustrate assignment of the amide linkage between the amino group of Asp and the carboxyl group of GalA residue E. Scheme 1. The structures of ... min), dried and analyzed by GC-MS with the standards prepared from D -GalN and (R)- and (S)-2-BuOH. The absolute configuration of L -aspartic acid was deter- mined by...

Ngày tải lên: 19/02/2014, 16:20

... Jingyue Yang 1,2 and Richard W. Gross 1,2,3,4 1 Division of Bioorganic Chemistry and Molecular Pharmacology, and Departments of 2 Medicine, 3 Chemistry and 4 Molecular Biology and Pharmacology, ... tissue w hich will further the understanding of the role of iPLA 2 sinenergy storage and utilization disorders of lipid metabolism such as diabetes, obesity and atherosclero...

Ngày tải lên: 19/02/2014, 16:20