Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
... ª 2008 The Authors Journal compilation ª 2008 FEBS
Molecular modeling and functional characterization
of the monomeric primase–polymerase domain from
the Sulfolobus solfataricus plasmid pIT3
Santina ... tri -functional monomeric primase–polymerase domain encoded by the
plasmid pIT3 from Sulfolobus solfataricus strain IT3 was identified using...
... Apart from
cleavage of the signal peptide and the propeptide,
which happens in the maturation process for every
conotoxin [8], the removal of the two additional resi-
dues Leu and Val at the C-terminus ... elution
conditions, the retention times of SFWT and S
FWT
were clearly not the same as the retention time of
the C-terminal tetrapeptide fragment from...
... mark the
boundary of the predicted substrate-binding pocket,
reveals that some of them are conserved in the inac-
tive isoform (i.e. Trp191 and Phe294). Even though
the precise role of these ... reduced the activity. It
can be hypothesized that these salts might chelate the
metal and efficiently remove it from the protein at
acidic pH.
Both the different sensitivity...
... chromatography. GST
isoforms 1-1, 2-2 were isolated from the C1E2 affinity
fraction; GST isoforms 3-3, 4-4 and 3-6 from the C1E1 frac-
tion; and GST isoforms 5-5 and 6-6 from the C2E1 fraction.
The purified ... for the two
substrates GSH and CDNB, except in the case of the
affinity for GSH of GST3-6 and GST 6-6. The 3-3 isoform
showed the highest affinity for...
... organelle and membrane proteins are synthe-
sized and folded with the assistance of molecular chap-
erones and other folding factors in the endoplasmic
reticulum (ER). In many cases, the process of ... redox-active
CGHC motifs within the a -domain and a¢ -domain.
Instead, they both contain the sequence CPRS in the
a -domain and CMNC or CINC in the a¢ -domain....
... interruption of the geometry
of the recognition helix for either Antp or the TTF-1
HD, the anomalous amide exchange pattern and the
NOE connectivity data of the C-terminal portion of
helix III ... homogeneity of the values of
the overall correlation time calculated from the individual (R
2
⁄ R
1
) ratios
suggested a good degree of isotropy of the global...
... homology
in their kinase domains and have a similar mode of
activation [1].
There are two isoforms of S6 kinase, S6K1 and 2.
Both have highly homologous kinase and kinase
extension domains flanked by the ... Akt and PDK1, mediates S6K activa-
tion [4]. Another major player in the activation of S6K
is the mammalian target of rapamycin, mTor (FRAP)
which senses the level...
... with the
higher inhibitory activity of the cEL peptide and the very
low inhibitory activity of the cYT peptide compared to
other peptides.
The flanking residue of the b-turn, i.e. Tyr3 of the cVY
peptide ... further docking
studies with flexible docking.
During flexible docking, the dihedrals of backbone of the
ligand were kept rigid, whereas the dihedrals of...
... heart. The molecular masses
of the observed polypeptides were in accordance with the
predicted molecular masses of the iPLA
2
c isoforms origin-
ating from translation initiation at each of the ... predicted
from northern blot analyses [26].
Identification of mitochondrial import sequences
in iPLA
2
c and demonstration of their functional integrity
Recent increa...
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denotes the number of words whose sense
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alternative word randomly from words in the same
deepest category of a Japanese thesaurus as the
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