Tài liệu Báo cáo khoa học: Substrate specificity of the pseudouridine synthase RluD in Escherichia coli doc

... residues in 16S rRNA to pseudouridine [9]. We have analyzed the substrate specificity of the pseudouridine synthase RluD in vivo by using 23S rRNA variants. Single point mutations were intro- duced ... enzymes, including RluD. RluD belongs to the RluA family [2]. Binding of RluA to one of its substrates, tRNA Phe anticodon stem-loop, induces reorganization of...

Ngày tải lên: 18/02/2014, 16:20

... further examined in order to determine the type of inhibition and the K i using brassinin (0.10–0.40 mm) as a sub- strate in the presence of inhibitor (0.10, 0.20 and 0.40 mm). The kinetics of inhibition ... BHAb. The mixture was incubated at 23 °C for 45 min in the presence of increasing concentrations of brassinin (0–1 m M). The curves obtained were fitted to t...

Ngày tải lên: 18/02/2014, 14:20

... enzymes remains to be elucidated. Several studies have highlighted the role of the N-ter- minal domain of UGTs in substrate specificity, and many lines of evidence indicated that it may contain the major ... together, the results of this study are consis- tent with the notion that residue 33 is involved in the interactions of the enzyme with the substrate in...

Ngày tải lên: 23/03/2014, 09:21

... proteins bind their cognate DNA-bind- ing site, they generally bind to other DNA-binding cofactors [10–12]. Meis2 is a member of the TALE superfamily of HD proteins, which are characterized by the ... by the interaction of Pbx1 with the Hth domain of Meis2. Target- ing of the Hth domain to DNA suggests that it is not a portable trans- acting repression domain. However, th...

Ngày tải lên: 16/02/2014, 15:20

... complex. The amino acid residues that determine the zymo- genicity of free FVIIa are as interesting as those involved in the TF-induced allostery. Amino acid resi- dues in FVIIa that are involved in the ... of these FVIIa variants is presumably linked to a more stable burial of the N-terminus of the protease domain in the activation pocket of the activation d...

Ngày tải lên: 18/02/2014, 08:20

... residue 283 is involved in DNA binding. However, residues 272 and 276 are close to DNA binding. The other four residues are further away, but at the same side of the protein, indicating a possible ... procedures. In the local minimiza- tion, the sum of the energy terms was minimized locally in the direction in which the total energy gradient was the steep- est....

Ngày tải lên: 18/02/2014, 11:20

... environments of BPA in the ligand-binding pocket of the ERRc. The proximity of each amino acid residue (within a distance of 5 A ˚ ) to BPA is shown in the boxes depicting the a -heli- ces. The portrait ... activity of the latter and to reverse the deactivating inverse agonist activity of 4-hydroxytamoxifen. However, the intrinsic binding mode of bisphenol...

Ngày tải lên: 18/02/2014, 16:20

... conserved in the sequence of saccharopepsin, the validity of this inter- pretation was examined by determination of inhibition constants for the interaction of the N-terminally exten- ded inhibitors ... ‘remote’ attachment of residues 35–81 from its own C-terminal segment, cause only minor perturbation of the inhibi- tory potency intrinsic to the N-terminal segment....

Ngày tải lên: 19/02/2014, 00:20

... identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS Torsten Neuhof 1 , Ralf Dieckmann 1, *, Irina S. Druzhinina 2 , Christian P. Kubicek 2 , Tiina Nakari-Seta ¨ la ¨ 3 , ... of successive deletions of terminal amino acid residues of all six hydrophobins providing this single match. A rapid means of identifying peaks corresponding to hyd...

Ngày tải lên: 19/02/2014, 02:20

... the anion-linked structural changes occurring in the protein. Thus, if the mutated residues seem to exert no relevant effect on protein stability, they play a role in shaping the pathway of the ... charges bind to spe- cific sites of horse cytochrome c [19,27]. To determine whether divalent anions bind to the same sites, we introduced some mutations within the site-contai...

Ngày tải lên: 19/02/2014, 05:20