Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

... and in the function of PAKs and sterol biosynthetic proteins in the same pathway(s). Therefore, it is conceivable that the Cdc42p effectors Ste20p and Cla4p may in uence sterol metabolism. Sterols ... that sterol biosynthesis may increase during formation of a mating projection [28]. Furthermore, homologues of oxysterol- binding proteins, a family of pro...

Ngày tải lên: 18/02/2014, 13:20

... ubiquinone-deficient strains and in the cyc1 mutant by measuring mRNA levels of stress-inducible genes and the phosphorylation level of the Spc1 MAP kinase. Induction of ctt1, encoding catalase, and ... Whereas induction of ctt1 + and apt1 occurred in all ubiquinone-deficient strains, induction of gpx1 + was not observed in any of the tested strains (Fig. 6)....

Ngày tải lên: 18/02/2014, 14:20

... line), in the presence of 100 l M neurotoxin ( • , dotted line), in the presence of 200 l M neurotoxin ( , dotted line) and in the presence of neurotoxin and 50 l M dopamine (h, dashed line). Modulation ... 1B shows the pattern seen after the development of the blot. With increase in time of incubation, the intensity of the band for the monomeric pr...

Ngày tải lên: 14/02/2014, 19:20

... significant production of P i in the vicinity of the ATPase within the matrix. CYPD ablation or its inhibition by cyclosporin A increases the rate of respiration stimulated by arsenate in intact mitochondria Regarding ... mediated by a relief of inhibition by cyclophilin D was evident in the form of slightly increased respiration rates during arsenolysis. Howe...

Ngày tải lên: 14/02/2014, 19:20

... with the amino acid arrangement observed in the catalytic site, indicate that C266 may constrain the nicotinamide and or the terminal tyrosine against the flavin. It has been described that the bind- ing ... acids participate in the fine-tuning of enzyme efficiency, modulating the interaction of Y308 and ⁄ or the nicotin- amide with the isoalloxazine. This type o...

Ngày tải lên: 18/02/2014, 17:20

... observed that the addition of ornithine or arginine to the culture medium cannot support the continuous growth of T. cruzi in the defined medium, as proliferation in the presence of these amino acids ... specific for the ADC gene, gave the results predicted. The radioactive bands obtained in the corresponding Southern blot assay (Fig. 3B) could only be explained b...

Ngày tải lên: 19/02/2014, 07:20

... gene expres- sion. In the light of these findings, we wished to determine the ability of the region ( )137 to )99 bp ) of the human cyclin D1 promoter, encompassing the Egr-1-binding site, to activate ... Biochem. 271) 4465 Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the e arliest eff...

Ngày tải lên: 19/02/2014, 16:20

... (83%). All together, recombination induced swapping of amino acids for 94 positions (19% of the residues) in the original LAC3 sequence. The C- and N-termini of the hybrids were 33–37% and 9–26% different, ... chimera, recombination preserved the integrity of domain D1 and the very end part of domain D3, two regions that interact precisely in the natural lacc...

Ngày tải lên: 18/02/2014, 06:20

... in enzyme-inhibitor affinity. In this way, the exact mechanism of endoxylanase inhi- bition by TAXI-I and the specific contributions of the amino acid residues at the interface of the enzyme– inhibitor ... 1A), resulting in the burial of 992 A ˚ 2 of accessible surface area. The imidazole ring of TAXI-I His374 that fits into the active site of the enzyme...

Ngày tải lên: 19/02/2014, 07:20

... available in these machine translations. The general lack of success in relating the judgments to some other possible indices of quality is also not surprising, since these indices, with the exception ... prepa- ration of the final text, and to D. B. Robinson, Jr., L. Rosier, and B. J. Kinsberg for their contributions to selection of passages and preparation o...

Ngày tải lên: 19/02/2014, 19:20