Tài liệu Báo cáo khoa học: 3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-c-dependent and mediated by the caspase-3-dependent apoptotic pathway doc

... ª 2009 The Authors Journal compilation ª 2009 FEBS 3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-c-dependent and mediated by the caspase-3-dependent ... ago- nist -induced apoptosis [26,27]. In the present study, we investigated PPARc agonist -induced adipocyte apopto- sis by using 3T3-L1 adip...

Ngày tải lên: 16/02/2014, 09:20

... expressed in the vascular tissues, such as the midrib of the cotyledon, the hypocotyl, and the root vasculature (Fig. 2E), and is upregulated by 100 lm K + (low-K + stress) and downregulated by 100 ... responses, and in the ABA sig- naling and stress -induced ABA biosynthetic pathways, respectively, in Arabidopsis [42–44]. Further studies revealed that CBL1 and...

Ngày tải lên: 14/02/2014, 18:20

... cells, the mutant was able to efficiently kill these cells. These data are consistent with the notion that induction of pyroptosis and apop- tosis by LF occurs through the cleavage of distinct substrates. Results ... others. Collectively, these effects dampen the innate and adaptive immune systems to allow Bacillus anthracis to survive and proliferate in the mammalian host....

Ngày tải lên: 16/02/2014, 09:20

... SJS260, and the creation of the ARE II ⁄ ARE IV double mutant used primers SJS261 and SJS262. The mutagenesis of ARE I and III introduced HindIII restriction sites and so the creation of the pTET- GLO 3¢ ... of the ARE I-AUUUA and ARE III-AUUUA sequences used primers SJS172 and SJS173, and SJS174 and SJS175, respectively. Mutation of the atypical AU-rich sequence...

Ngày tải lên: 16/02/2014, 09:20

... modulate the levels of these proteins: these include the coupling of the toxin to the antitoxin synthesis and the limited degradation of the polycistronic parD mes- senger, which gives rise to ... on the one hand enhances the fluorescence of the internal Trp residue of the toxin, indicating a local distortion in the structure and, on the other hand, inactivates growt...

Ngày tải lên: 18/02/2014, 04:20

... for GST and GST-fused proteins (GST– IST1, GST–IST1MIM, GST–IST1MIM L326D,L353A , GST– CHMP6NT, GST–pHis and GST–MIT–pHis). Expression of GST–IST1, GST–IST1MIM and GST–IST1 L326D,L353A was induced ... not enhance the autolysis of mGFP–calpain 7 in cultured cells. On the other hand, overexpression of FLAG– IST1 DMIM1,2 suppressed the autolysis. This observation might be explained...

Ngày tải lên: 18/02/2014, 04:20

... shock. This is achieved by HSF1 and the his- tone deacetylases HDAC1 and HDAC2 [77]. In heat- shocked cells, HSF1 binds to HDAC1 and HDAC2, and their histone-deacetylase activities increase. The heat -induced ... com- plex, the third helix of the DBD is docked into the major groove, approximately perpendicular to the heli- cal axis of the DNA. The evolutionarily...

Ngày tải lên: 18/02/2014, 04:20

... ATH1_3 and ATH1_4) using ATH1_1 and ATH1_4 as external primers. This final HA- tagged PCR fragment was cloned into the pGEM-T-easy vector and was then excised by SnaBI ⁄ AgeI to replace the SnaBI ... targeting. Together, these results confirmed that trehalase in the vacuole is likely prompted to partial degradation and is not required for cell growth on trehalose. The TM d...

Ngày tải lên: 18/02/2014, 06:20

... post-transcriptional control exerted by miRNAs. miRNAs and the circadian cycle miR-219 is capable of shortening the circadian period by 10–20 min [12]. The exact mechanism by which this miRNA is able to alter the circadian ... experiments, these mutated 3¢UTRs were com- pletely refractory to regulation by the miR-192 ⁄ 194 cluster, indicating the direct suppression o...

Ngày tải lên: 18/02/2014, 06:20

... is not the natural RNA target in Arabidopsis, the Box B sequence should mimic the authentic Arabidopsis targets. On the other hand, APUM-7 was unable to bind to NRE, possibly as a result of the ... all of them (sense and antisense) bound to bait specifically, suggesting the existence of a consensus motif within these 63 distinct transcripts recognized by APUM-2. We therefore...

Ngày tải lên: 18/02/2014, 06:20