Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc

... microdomains of the plasmamembrane (caveolae), intracellular organelles, ligand-independent activation of growth factor- and cytokinereceptors and autocrine stimulation of signal transduc-tion by release ... under-lying the RVI, and thereby increase hepatocyte shrinkage by hyper-osmolarity. Mutual amplification of swelling and oxidative stressmay be limited by the hepatocyte’s antioxidant and volume-regula-tory ... the regulation of metabolism, gene expression and the susceptibilityto different kinds of stress [6]. For example, the inhibi-tion of autophagic proteolysis by insulin, glutamine and ethanol in...

... identification of new metabolites of vitamin D3 made by the action of P450scc willenable us to proceed with further biological testing of these compounds and the investigation of whether thispathway ... Miller2 and Andrzej Slominski41 School of Biomolecular, Biomedical and Chemical Sciences, The University of Western Australia, Crawley, Australia2 Department of Pharmaceutical Sciences, College of ... hydroxylgroups are present at positions 20 and 23. Three minor products of D3metabolism by P450scc were identified by MS and by examining their sub-sequent metabolism by P450scc. These products were 23-hydroxyvitaminD3,...

... mixingtimes of 80 ms and 40 ms, respectively [53,54].Analysis and modeling of sequences and structuresWe used Chothia’s scheme for the numbering of the amino-acid sequences of immunoglobulins and ... fragments and the viral antigen (serotype 1)were measured by a competition ELISA in solution and their kinetics of interaction by surface plasmon resonance. The diversity and junction resi-dues of ... CDR3 loops of VH and VLcontain the residues of diversity and junction,encoded by the D segment, and the VH-D, D-JH, and VL-JLjunctions [17]. They are located at the center of the antibody...

... filtered by a minimum size of 100 amino acid residues and flanked by start and stop codons. These homology searches resultedin nearly 1000 redundant amino acid sequences which werefollowed by an ... in annotation of new sequences and model the unique motifs of a family. The patterns weregenerated by the program pratt version 2.1 [55,56]. prattwas run on sequences from each of the MGST1-3, ... °C and the extension prod-uct was PCR amplified by the addition of sense primer and 2.5 units of Taq polymerase (Pharmacia Biotech). The tem-perature cycles were 95 °C, 1 min, 55 °C, 1 min and...

... work was supported by the Thailand ResearchFund for Young Researchers (TRG-4580058), by agrant from Suranaree University of Technology (SUT-1-102-46-48-06), and by a grant from the German Aca-demic ... 2005)doi:10.1111/j.1742-4658.2005.04753.xThe enzymatic properties of chitinase A from Vibrio carchariae have beenstudied in detail by using combined HPLC and electrospray MS. Thisapproach allowed the separation of a and b anomers and ... judged by the patterns of the product formation, the release of dimers, trimers and tetramers from the hexamer wasconsidered to result from direct action of the enzymes.On the other hand, the...

... Carbohydrate,glycolysisLactococcuslactisModeling Pi explicitly and regulation of pyruvate kinase (by Pi and FBP),PFK (by PEP) and GAPDH (by NADH) are critical to describeobservations (rapid increase in PEP,Pi and gradual decrease ... withregulation of pyruvate decarboxylase, a reversiblealcohol dehydrogenase, and drainage of pyruvate. Using the method of time rescaling in the extendedmodel, the description of thetransient ... produced by the oxidativePPP accounts for at most 44% of the NADPH and 22% of totalreductant needed for fatty acid synthesis Stoich ss, fitEXCEL NG Single time point of metabolites measured by GC-MS...

... withbinding of both MOF and Tip60 (equivalent to Esa1in yeast NuA4) [124,130]. However, in human cellsdepletion of MOF, but not Tip60, results in reducedglobal levels of AcH4-K16 and defective ... acetylation and deacetyla-tion, and transient recruitment of remodellers and transcription factors.A cycle of transcription commences with the recruit-ment of transcription factors and co-factors ... these advanceshave been accompanied by a relative decrease in thenumber of studies aimed at gaining an understanding of the structural conformation of chromatin, and thechanges in chromatin structure...

... grants from the Scientific Fund of the Ministry of Education,Science and Culture of Japan (G.T.); the Program forPromotion of Fundamental Studies in Health Sciences of the National Institute of ... expression of estrogen receptor-a(ERa) is autoregulated by miR-18a, -19b and -20b,which in turn are upregulated by the activation of ERa. This mechanism of regulation provides a widerange of coordinated ... upstream from the site of initiation of miR-155 transcription [19]. We previ-ously reported that simulation with nerve growth fac-tor induced the expression of miR-221 and miR-222 inPC12 cells, and...

... deepening our understanding of diseases.Materials and methodsTyping of miRNAs by positional relationship tomRNA transcriptsInformation about the localization and strand direction of 939 miRNAs, ... nonhistone pro-teins: p53 and Myo-D are targeted by HDAC-1,whereas Bcl-6, Stat3 and YY1 are targeted by HDAC-2. By regulating both histone and nonhistone proteins,HDACs 1 and 2, classified as class ... Targets of mature miR-124 include the3¢ UTR of CDK6, an oncogene. Epigenetically mask-ing of miR-124 induces activation of CDK6 and conse-quent phosphorylation of Rb at serine residues 807and...