Tài liệu Báo cáo khoa học: Chromatin under mechanical stress: from single 30 nm fibers to single nucleosomes pdf

... claim to original French government works 2243 MINIREVIEW Chromatin under mechanical stress: from single 30 nm fibers to single nucleosomes Jan Bednar 1,2,3 and Stefan Dimitrov 4 1 CNRS, Laboratoire ... nucleosomal arrays and 30 nm chromatin fibers. Chromatin samples ‘eligible’ for single molecule experiments All micromechanical experiments applied to...

Ngày tải lên: 14/02/2014, 18:20

... leading to microvillus effacement [67]. Although the precise sequence of events that leads to secretion of translocators is not well understood, it is of note that IpaC has been shown to localize to ... between four and 20 effec- tors with dramatic effects on the target cell, leading, for example, to cytoskeleton rearrangement, membrane disruption or the initiation of apoptosis [1–3...

Ngày tải lên: 14/02/2014, 22:20

... Major virulence factors that contribute to the inflammatory response and to epithelial cell damage have been iden- tified, among them cytotoxin-associated gene protein A [4,5], vacuolating toxin A [6,7], ... specific fatty acids or amides from the environ- ment, either to supply the bacterium with the fatty acids necessary for its metabolism, or to protect and detoxify it from the de...

Ngày tải lên: 16/02/2014, 14:20

... is specifically induced under oxidative stress, we nemed this protein OsiS. Spectrophotometric properties of OsiS To confirm the activity of OsiS predicted from the sequence-alignment data presented ... able to transfer sulfur to apoferredoxin in vitro. The three cysteine desulfurases from E. coli have been found to catalyze abortive transamination reac- tions, which convert the l...

Ngày tải lên: 18/02/2014, 04:20

... ngu' chinh la noi lull gid ky dc cua dan toe cung vo van thong tin van hoa, la tam guOng phan chieu lich su" hinh thanh vung dat, toe ngddi, phddng thdc song va sinh hoat van ... nghiet de tru vdng va ton tai cua nhdng cd dan Thanh Hoa trong sudt chieu dai lich sd ddng nddc va bao ve dat nddc. Trang sudt dam dai "di md cdi" cua lich sd dan toe, "tien...

Ngày tải lên: 18/02/2014, 05:20

... generated by photo- activation is dissociated from opsin and converted to all-trans-retinol by retinol dehydrogenase [6]. The all-trans-retinol is then exported from photoreceptors to the retinal ... RPE65 FEBS Journal 276 (2009) 302 0 303 0 ª 2009 The Authors Journal compilation ª 2009 FEBS 302 9 25 Bernstein PS, Law WC & Rando RR (1987) Isomeri- zation of all-trans-retinoids...

Ngày tải lên: 18/02/2014, 08:20

... adjusted relative to one of the signals, as designated, to calculate the relative band density. Vector description and construction All DmEcR and DmUSP expression vectors and the lucif- erase (and b-galactosidase) ... were purified from an agarose gel excision, and then ligated to create the vector pcDNA3.1 + LdEcRA. The LdEcRB fragment was removed from pBluescriptKS + LdEcRB [31], a...

Ngày tải lên: 18/02/2014, 08:20

... were per- formed at 30 °C. The reaction was initiated by the addition of GTP to a predetermined concentration (0.017–1.7 mm). The assay was monitored photometrically at 310 nm. Reac- tion rates ... apparent 0.160 0.120 0.080 0.040 0.0 240 260 280 300 320 340 360 380 400 Wavelength (nm) Absorbance 0.1 0.5 1 10 50 Time (s) 5 Fig. 6. Optical spectra from a stopped-flow experiment with wi...

Ngày tải lên: 18/02/2014, 11:20

... transcriptional regulation in eukaryotes is poorly understood [1]. Among the most intensively studied transcription factors is heat shock factor 1 (HSF1). HSF1 binding to its response elements in target gene ... quantifying transcription factor–DNA binding. Our goal was to develop a fast and versatile assay for detecting and quantifying transcription factor– EMSA AB TransLISA HSE P B 30...

Ngày tải lên: 18/02/2014, 14:20

... (total) 12020 10539 No. reflections (working set) 11438 9976 No. reflections (test set) 582 563 No. atoms 2702 2706 Resolution (A ˚ ) 30 2.6 (2.67–2.60) 30 2.7 (2.77–2.70) R factor (%) 24.7 (30. 1) ... occurs by binding of the inhibitor to an allosteric site in competition with the activator dCTP [7]. For dCTP deaminase, the mechanism of dTTP regulation is not understood. Only dCTP deaminas...

Ngày tải lên: 18/02/2014, 16:20