- Thu hoi:
Thdi gian nghien ciiru: 12 thang Thdi gian bat dau: 3/2006 Thdi gian bat dau: 3/2006 Thdi gian ket thuc; 3/2007
Ten cac can bo phoi hop nghien curu:
D6 tai CO sir tham gia cua NCS. Le Huyen Tram, ThS. D6 Quoc Hung,
CN. Nguyen Thi TuS, Phdng thf nghiem Hoa hoc cac hop chat thien nhien, Khoa Hoa hoc, Trudng Dai hoc Khoa hoc tu nhien Khoa Hoa hoc, Trudng Dai hoc Khoa hoc tu nhien
So dang ky de tai
Ngay:
So chiing nhan dang ky ket qua nghien cuu: ket qua nghien cuu:
Bao mat:
a. Ph6 bien rong rai: x b. Ph6 bien han che: b. Ph6 bien han che: c. Bao mat:
Tom tat ket qua nghien cufu:
1. Da tien hanh sang loc lua chon cay Nghe xanh (Curcuma aff. aeruginosa
Roxb., Zingiberaceae), mot cay thuoc mdi duac phat hien, dang duoc trong
dai tra, va duoc sir dimg trong Y hoc dan toe d^ dieu tri cac benh nha dau bung va tang cudng tieu hoa lam doi tuong nghien cihi cua de tai. Da tien hanh giam dinh thirc vat va thu thap nguyen lieu thirc vat phiic vu cho nghien cihi.
2. Da xay dung duoc cac qui trinh chiet va phan tach chon loc de dieu che cac
ph^n chiet co hoat tfnh sinh hoc, /z-hexan (hitu sua't 3,14% so vdi luong
nguyen lieu kho), etyl axetat (0,6%) va /i-butanol (0,14%).
3. Mot phucmg phap phan lap sac ky thfch hop da duoc phat trien de nghien ciJu mot each he thong ve hoa hoc va hoat tfnh sinh hoc ciia phdn chiet /(-hexan tir
than rl Nghe xanh. Bang phuong phap nay cac hop cha't thanh phdn chfnh
curdion va zederon da duoc phan lap vdi cac hieu su^t phan lap tuong img la 0,1% va 0,05% so vdi luong nguyen li^u khd ban ddu. Phuong phap nay cung
thfch hgp di phat tri^n thanh mgt phuong phap phan lap lugng ldn cac hgp
cha't cd hoat tfnh sinh hgc cao la curdion va zederon, cung nhu cac che pham dugc chtia cac nhdm sesquiterpenoid khac nhau.
4. Cac ket qua nghien ciJu cac hgp chat de bay hoi cua Nghe xanh da xac dinh dugc tdng so' 23 hgp chS^t (chii yeu la cac sesquiterpenoid) vdi P-elemen - mot hgp cha't cd hoat tfnh chdng ung thu la thanh phdn chfnh cua cac sesquiteipen hydrocarbon, va curzerenon, curcumol, germacron va curdion la cac thanh phdn chfnh ciia cac sesquiterpenoid chiia oxy, trong sd dd curzerenon da dugc
sir dung lam thudc lam sang cho ung thu da va c6 tir cung.
5. Cac ket qua nghien ctiu cac hgp cha't phan cue hon da ddn den viec phan lap
mirdi bdn hgp chat tinh khiet tir than re Nghe xanh, curdion , axit palmitic,
zederon, yO^sitosterol, stigmasterol, 13-hydroxygermacron, aeruginolacton, guaianediol, isoprocurcumenol, procurcumenol, aerugidiol, aeruginofuran, zedoarofuran va aeruginon. Nhu vay cac hgp cha't dugc phan lap cd ca'u true cua cac sesquiterpenoid, cac steroid va axit beo.
Cac sesquiterpenoid aeruginolacton va aeruginofuran la cac hgp chat hiiru ca
thien nhien mdi, va aeruginon la hgp chat lan dau tien dugc phan lap tu thien nhien.
6. Trong cac thir nghiem theo huang khang khuan va khang nam cac phdn chiet n-
hexan, etyl axetat va //-butanol da dU(:*c danh gia la cd hoat tfnh khang nam. Chiing
deu thi hien lac dung khang Aspergillus niger, Candida albicans va Fusarium
oxysporum d miic MIC tir 25-200 ^g/ml. Cac phan tach cd dinh hudng khang
k h u ^ va khang ndm da ddn den vice phan lap cac hoat chat la curdion va zederon. - Curdion va zederon (cac MIC 25 ^lg/m]) cd thd dugc phat tricn thanh cac tac
nhan chdng benh thdi re d thuc vat gay ha\ ndm mdc Fusarium oxysporum.
gay benh cho ngudi tucfng iing la Aspergillus niger (MIC 50 |ig/ml) va
Candida albicans (MIC 25 |ag/ml).
- Curdion (MIC 50 |ag/ml), phdn chiet etyl axetat (MIC 200 |ig/ml) va phdn chiet /2-butanol (MIC 50 |ig/ml) cd hoat tfnh khang vi khuan
Staphylococcus aureus. Chung cd the la nhimg ddi tugng nghien ciiu thii vi
cho hudng tim kiem cac hgp chat cd hoat tfnh khang Staphylococcus
aureus khang methicillin (MRSA) va vancomycin (VRSA).
7. Da phan lap va xac dinh dugc cac hgp cha't cd hoat tfnh bao ve gan ti'ong than
rt Nghe xanh nhu furanodien, germacron, curdion, 13-hydroxygermacron va
aerugidiol. Furanodien va 13-hydroxygermacron cung la cac hgp chat da dugc chiing td la cd tac dung lie che sir san sinh cua nito oxit (NO).
8. Trong cac thii nghiem ve hoat tfnh chdng oxy hoa (don gdc tu do) va gay dgc te bao ddi vdi ba ddng te bao ung thu ngudi la Hep-G2, Fl va RD, ca curdion va zederon deu khong the hien cac hoat tfnh dang chu y.
9. Cac ke't qua nghien cihi cua de tai da ddn den sir chuan bi cong bd hai bai bao
tren cac tap chf cua Viet Nam Dugc hpc va qudc te Chemical and
Pharmaceutical Bulletin, Mot sinh vien da tdt nghiep va ba sinh vien nam thii
3 da hoan thanh nghien ciiu khoa hgc trong khuon khd cua de tai nay.
Kien nghi ve quy mo va doi tugng ap dyng nghien ciiu:
Cac ke't qua nghien cihi cua de tai nay cd the dugc phat trien trong cac nghien ciiu dieu che cac che pham dugc va cac tac dung cua chung trong viec bao ve gan va dieu tri cac benh viem.
Ho ten
Chu nhiem de lai
Phan Minh Giang
Thii truang co quan chii tri dc tai
i}il} I/LUI tdf
Chu tich Hoi dong danh gia chinh thuc
/r^h.' Jl'u / 4 /h'
Thii truong co quan