- ; khdng co tdc dung khdng vi sinh vdt kiem dinh
CURCUMA AFF AERUGINOSA ROXB.)
KHOA LUAN TOT NGHIEP HE DAI HOC CHINH QUI
Nganh: Hoa hoc huu co
Can ho Intani; ddn : GS TSKH Phan Tong Sffn
TS Phan Minh Giang
Sinh vien : Nguyen Thi Tue
DlSOC HOC, 2006
Nghien curu ph§n lap va khao sat hoat tinh sinh hoc ciia cac sesquiterpenoid tir than r^
Nghe xanh (Curcuma aff. aeruginosa Roxb., Zingiberaceae)
Phan Minh Giang, Nguyin Thi Tue, Dao Quoc Hiing, Phan Tong Som
Phdng thi nghiem Hod hgc cdc hap chd't thien nhien, Khoa Hda hgc, Trudng Dgi hgc Khoa hgc tu nhiin, Dgi hgc Qud'c gia Hd Ndi
1. Dat van dl
Nghfi xanh (Curcuma aff. aeruginosa Roxb., Zingiberaceae) la loai c^y thao cao tur 1- 2 m. VS mat thuc vat hoc cay ngh6 xanh tuong tu nhu cay ngh^ len d6ng {Curcuma aeruginosa
Roxb., Zingiberaceae) [1]. TXiy nhifin, diim kh^c biSt giGa nghe xanh va nghe ten d6ng la lat
cat ctia than rk nghe xanh th3m hon cua nghfi ten d6ng, hoi nga sang mau xanh muc Cuu Long. Hien nay cay duoc tr6ng b Wh. N6i, Vinh Phue va Hai Phong dl thu than rl lam ihuO'c
chiia cdc benh nhu dau bung v^ an u^ng kh6ng tifiu.
Cdc nghien cdn d^u tien vl th^nh p h ^ hod hoc da xdc dinh duoc su tap trung cAc
sesquiterpenoid trong than rl nghe xanh v6i curdion va zederon c6 ihl la cac thanh phan
chinh [2]. Curdion duoc ihdng bdo 1^ c6 hoai tinh bao ve gan [3]. Cdc nghien cihi nay cho tha'y
triln vong md r6ng cdc nghien cuu hod hpc m6l cdch c6 he th6ng vl cac thanh p h ^ sesquiterpenoid til than rl nghe xanh cung nhu cdc hoat tinh sinh hoc cua chung. Dilu nay dat yeu cdu phdt triln m6l phuong phdp sac k^ ihich hop dl phan lap cdc sesquiterpenoid tu than
rl nghe xanh, dac biet Id cdc thdnh phdn chinh nhu curdion va zederon 6 luong \6t\ dl cho cac
chuyin hod hod hoc vd nghien cihi vl hoat linh sinh hoc. Cdc hoat tinh khdng khuin, khang ndm, chtfng oxy h6a vd gay d6c llbdo cua curdion vd zederon cung da dugc khao sdt va th6ng bdo trong nghien cuu ndy.
2. Phucmg phap nghien curu va dieu kien thuc nghiem
2.1 Phuang phap va thiet bj phan tich, phan lap va khao sat cau true cac hop chat
Sdc k^ Idp m6ng dugc thuc hien tren ban mong trdng sdn Merck AlufoUen DC 60 F254- Cac vei cha't dugc phdi hien bang cdch phun thu6c thi vaniIin/H2S04 1% sau d6 ho nong vd bang
d^n t i ngoai b budc sdng 254 nm. Sac k^' cdt (CC) dugc thuc hiSn dudi trong luc cua dung mdi vdi cha't hd'p phu Id silica gel Merck (cd hat 63-200 \xm).
Ph6 hdng ngoai (IR) dugc do tren may Impact-410-Nicolet FT-IR. Phd khdi luong va cham dien lur (EI-MS) dugc do tren mdy HP-5898 B MS. Ph6 cdng hudng tiic proton ('H-NMI^) va cacbon 13 (^'C-NMR) dugc ghi tren mdy Bruker AV 500. Sac ky khf-kh6i ph6 kef lien hop (GC-MS) dugc thuc hien tren GC HP 5890 II, cdt mao quan HP-5 (30 m x 0,25 mm dudng kinh Irong; Idp phim day 0,25 jim) lien hgp vdi MSD 5989B, chuong trinh nhiet dd 45°C- 150^C, lO'^C/phut, dCtng I phiit, sau dd l"50'C-250'C, 6"C/phut, dung 15 phut. Nhiet dd injector: 250*'C. Nhiet dd detector: 280^C Khi mang: He.
2.2 Phucmg phap thur hoat tinh sinh hoc
2.2.7 Thu hogt tinh khdng khudn vd khdng ndm
Hoat linh khdng khu& va khdng ndm dugc sang loc tren phi^n vi lugng 96 gie'ng. Cac mAu thir cho ke't qua duong tinh dugc danh gia ndng dd lic che' tdi thilu(MIC) tren phi^n vi lugng 96 gilng vdi cdc khdng sinh kilm dinh bao gdm Ampicillin, Tetracyclin va Nystatin [4].
2,2.2 Thu^ hogt tinh chdng oxy hod in vitro
Hoat linh chd'ng oxy hod dugc ddnh gid bdi phuong phdp don gdc 2,2-diphenyM- picrylhydrazyl (DPPH) trong dung dich etanol bao hod [5].
2.2 3 Thu tdc dung gdy ddc tebdo
Tdc dung gay ddc l l bdo dugc thit theo phuctng phdp dang dugc thuc hien tai Vien nghien cuu ung thu Qudc gia Hoa K^ (NCI) [6] vdi 3 dong t l bdo ung thu ngucri Id Hep-G2 (ung thu gan), FI (ung thu mdng tir cung) vd RD (ung ihu mdng tim).
2.3 Nguyen Heu
) i r q c HOC, 2006
20 kg than rl nghe xanh tucfi dugc ria sach, thdi I^t mong, phoi khd trong bdng dam, sd'y d