Da bao vf vdi dilm xuit sic nim
OANH MUC CAC HINH
Hinh 1.1. c i u tnic eiia mot phin ni khing huylt thanh 18 Hinh 1.2. So đ cac chuoi cua mot khang huyet thanh 19 Hinh 1.3. Cicd^c t6 cua vi khuan ben canh mgt te bao co till 20
Hinh 1.4. Cic dOc t l tren bj trung hda bdi khang huylt thanh 21 Hinh 1.5. Khang huylt thanh don đng, lien kit vdi mpt epitope d ^ hifu 21
Hinh 1.6. Cic khing huylt thanh da đng, moi khang hw/k thanh lien kit vdi mpt epitope khic nhau 22
Hinh 1.7. Cautaocijahat viriitSdi 25 Hinh 1.8. Hinh minh hpa ciu tnic cua virut Rota 34 Hinh 1.9. S\r nhan I§n ciia virut Rota trong tl bio 39 Hinh 1.10. Hinh inh thd tai Vỉt Nam 40 Hinh l.Ụ Hinh inh din thd tai Vỉt Nam 42 Hinh 2.1. Hinh inh liy miu tmh m?ch tai 49 Hinh 2.2. Hinh inh tiem trong da 50 Hinh 2.3. Hinh inh liy miu dpng mach cinh. 51 Hinh 2.4.ạ CPE (+): dim te bio bj phi vd vach biln thanh tl bio khing l6 (huy hỏi t l bio) 57
Hinh 2.4.b. CPE (-): tl bio van kin 1 Idp, hinh dang, vien tl bio binh thudng (nhu gilng đi chiiung tl bio) 57
Hinh 2.5. Hinh inh virut Rota dudi kinh hien vi huynh quang 67 Hinh 3.1. N^ng dO khing huylt thanh Sdi thu dupe lin 1 gay dap ung miln dich 75
Hinh 3.2. N6ng dO khing huylt thanh Sdi thu dupe d dpt tiem 2 77 Hinh 3.3. Ning dp khing huylt thanh Sdi thu dupe tai lan 3 gay dap iing mien dich 78
Hinh 3.4. Hi§u gii khing huylt thanh B ^ lỉt typ 1 tai cic thdi diem liy miu 82 Hinh 3.6. Miie đ khing huylt thanh Bji lỉt typ 1 cheo typ 3 84 Hinh 3.7. Hi§u gii khing huylt thanh typ 2 sau khi giy miln dich 5 lieu lien tilp 86
Hinh 3.8. N&ng d^ khing huylt thanh B ^ lỉt typ 2 trung hda vdi virut Rota typ 1 87 Hinh 3.9. N^ng dO khing huylt thanh B ^ lỉt typ 2 tnmg hda vdi virut Rota typ 3 89 Hinh 3.10. Hifu gii huyet thanh B ^ lift typ3 trong qua trinh gay dip Omg mien dich 91 Hinh 3.11. Huylt thanh thd typ 3 trung hda virut Rota typ 1 trong qua trinh giy dip iing mien dich 92 Hinh 3.12. Huylt thanh thd typ 3 trung hda virut Rota typ 2 trong qui trinh gay dip iing miln dich 94
Hinh 3.13. Hifu gii khing huyet thanh IgG thd sau 5 lilu giy dip ung miln dich 97 Hinh 3.14. Hifu gii khing huylt thanh IgG thd sau 6 lilu gay dap iing mien dich(M) 100
DAT VAN Bt
Trung t ^ Nghien cuu San xuat Vac xin va Sinh pham Y te hay Trung tam Nghien cuu San xuat vac xin Sabin duoc thinh lap ttr nam 1994 co nhiem vu san xuat va kiem dinh vac xin. Hien nay Trung tam dang san xuit va kiem dinh vie xin Bai liet, vac xin Soi; nghien cuu, san xuat vac xin Rota phong tieu chay cho tre em. He th6ng kilm dinh cua Trung Tam duoc dao tao co ban va chuyen sau phuc vu cong tac kiem dinh chit luong vie xin. Mot trong nhimg nguyen vat Ueu ca ban sir dung danh gia an toan ciia vac xin ban thanh phim trong phong thi ngliiem la huyet thanh khang Soi, khang virut Rota, va khang virut Rotạ Khang huylt thanh nay sur dung cho thu nghiem tim tac nhan ngoai lai trong h6n dich vac xin san xuit. D6ng thoi khang huylt thanh nay cung sir
dyng dk chuin dp hieu gid vie xin Rota va vie xin Bai liet thanh phim. Nhting san phim nay truoc
day du^c cung cap hoac mua tir cac nhi san xuat Nhat, Mỵ Tuy nhien de chu dong trong cong vif c, Trung tam thyc hien de tai voi nhimg muc dich sau:
1. iTng dung va hoan thien qui trinh san xuat huyet thanh dSc hieu virut Sdi, Bai liet. Rota su dyng trong kiem djnh vie xin;
2. Sdn xuit dugc huylt thanh khang virut Soị Bai liet. Rota dat tieu chuan ca so;
3. Danh gia chit lu<;mg huylt thanh ve tinh đc hieu, hieu gia so v6i huyet thanh nh$p ngogi
CHirOfNG Ị TONG QUAN 1.1. Khang nguyen
Khang nguyen la nhung phan tCr 1^ hoac vat la, thucmg la cac protein, kid xam nhap vao co the chii tU co kha nang kich thich co the chu sinh ra cac dap ung mien dich dac hieu ch6ng lai chiing.
1.1.1, Tinh sinh miin dich va tinh khdng nguyen
Tinh sinh miln dich vi tinh khang nguyen la hai pham tru hen quan din nhau nhung khac hinnhaụ
Tinh sinh mien djch Id kha nang kich thich sinh ra dap ting miln dich dich thi hoac dap iing mien djch qua trung gian te bao dac hieu voi khang nguyen:
Te bdo B + Khang nguyen ® Ddp Omg miln dich dich thi
Te bao T + Khdng nguySn ® Ddp iing miln dich qua trung gian tl bao
Voi y nghia nay chiing ta co the goi cdc khdng nguyen duoi mgt tSn khde chinh xac hon la chat sinh miin djch.
Tinh khdng nguySn Id kha nang kit h<?p mot cdch dac hifu ciia khdng nguygn v6i cac san phdm cuoi ciing cua cdc dap iing tren (tiic la voi khdng huylt thanh trong dap iing miin dich dich the ho§c cdc thu the ciia te bdo lympho T danh cho khang nguyen trong đp img miln dich qua trung gian ti bdo).
MEIC dii tat cd cdc phdn tit co tinh sinh mien djch thi deu co tinh khang nguyen. Nhung ngugrc l£u khong phdi bat ký m^t phan tii ndo co tinh khdng nguyen thi ciing diu co tinh sinh miin dich. M$t so phan tu dupe gpi la hapten co tinh khang nguyen nhimg bdn than chiing khong co kha ndng kich thich sinh ra m^t đp iing mien dich dac hifụ Noi mot cdch khac cac hapten co tinh khdng nguyen nhung kh6ng co tinh sinh miin djch. Khi hapten dugc gin voi m^t protein thich hgp ndo d6 thi phirc hgp hapten-protein nay lai tro nSn co tinh sinh mien dich, va dap iing miin dich do phiic hgp ndy kich thich sinh ra chii yeu la chong lai va mang tinh dac hieu voi phin hapten; phan tu protein gin voi hapten nhu vay dugc ggi la protein tdị Nhu vdy, phiic hgp hapten-protem tdi la chit sinh mien dich hoan chinh voi hai yeu cau can va du Id tinh khdng nguyen (do hapten cung cip) vd tinh sinh mien djch (do protein tai cung cap). Rat nhieu chat quan trgng vl phuong difn mien djch hgc bao gom thuoc, cdc hormon peptide vd cdc hormon steroid co the hoat dgng nhu cac hapten.
1.1.2. Cdcyiu to anh hinmg den tinh sinh mien dich
1.1.2.1. NhQng tinh chat cua ban thSn kh^ng nguyen anh hu-ong den tinh sinh miln djch 2
Co bon dac diem cua chit sinh mien dich gop phin quy6t dinh tinh sinh miin dich ciia no
do la tinh la, kich thuac phdn tie, thanh phin vd tinh khong thudn nhdt ve phuong dien hod hoc, va kha ndng gidng hod dl co ttie dugc xir ly va trinh dien cimg voi mot phan tir MHC tren mang tl (adsbygoogle = window.adsbygoogle || []).push({});
bdo trinh difn khdng nguyen hoac tl bao ciia ca thi bi biln đị