Bang 7. Ciich Q,inh gij muc of! (+J cua slw phfJ.'m peR
Tinh chat bang ADN di~n di tren Danh gia
th<;lch ke't qua
Kh6ng co bang ADN (-J
Sang ADN mo hoae eha'm hat
(+)
lhallh hang
Bang ADN nha ho~e vLla, nhin r6 (++J
Bang ADI'J lan, c1jm Ilhin rAt ro (+++J
TCNCYH 56 (4) -2008
III. KET QUA
1. Ket qua kie'm tra cae chung DEC trong nghien cuu
Cac chClng DEC sCI d~lng trong nghien cClu clLfQe kiem tra bang llu6i dy, phan I~p (I'~CPLJ, xac cli nh tinh cha-t sinh v~t hoa hoc (SVHH) va PCR.
Bang 2. K~t qua kiem tra cae ehUng vi khuan slt d/',mg trong nghien cc.ru
.. -.- - - -
STT Chung DEC m5u NCPL - SVHH Ket qua PCR
ETEC ATCC 35401 E. coli eltS, eSIA (+)
2 EHEC ATCC 43890 E. coli vtl, eaeA (+)
··_-----. _P- - - --- -
3 EHEC ATCC 43889 E. coli v12, eaeA (+)
._ - -
4 EPEC ATCC 4388 7 E. coli eaeA, bfpA (+)
5 EIEC ATCC 43893 E. coli lal (+)
- - - - - - -
6 EAEC 97R E. coli pCVD (+)
- ... _--------.- -
7 E. cali ATCC 11 775 E. coli (-) voi 13 e~p m6i cJ~e hieLi eha DEC - - - - - -
Ke'l qua abang 2 cllo thay, cac chting DEC Sti' d(lng Irong nghlen nfu cl,i c/trqc kle'm tra vii clting vol
len cua no.
2. Ket qua ki~m tra cac mIiu phan
PCR kiem tra 10 maLi phan ehoket qLla am tinh, cae maLi phan nay khong co DEC.
3. Phat trien, hoim thi~n quy trinh tach chiet tn!c tiep ADN cua vi khuan tU phan tn!c tiep ADN cua vi khuan tU phan
3. 1. TOI u'u hoa Iu'q'ng nu'O'c ca't hail tan
200mg phan
- 200 mg mau phan c1a ehuan bl eha vaa cac
tube nlwa chVJ 1, 2, 3, 4 va 5 ml mloc ea't. uk
tren may lac c1e'n khi phan duac haa tan thanh
mot huyen dlch.