tach chiet dn khoang 90 - 120 phut.
So vai th iet ke ban d~u (Iy ta mal 00 g x 10m, sall do 700 g x 10m, 12000 g x 10m nhtl bang 3), quy trinh t6i uti cho ket qua tot. Eli~u nay co the c1L(<;IC giai thich nhL( sau: 0 toc dO 700 g, mot 50' vi khllan c1a bi I~ng, VI v~y, vi~c bo c~n la'y ntlac nOi (; bL(6c nay co th~ lam mat vi khuan, dan Mn ket qua PCR dtlong tfnh yeu ho~c am tinh.
oVi~t Nam, day 103 nghien cuu d~u tien va 103 mot trong 50' ft cac nghien cliu tren the giGi. de mot trong 50' ft cac nghien cliu tren the giGi. de
c~p Mn va'n de nay.
V. KET lU~N
Ky thu~t tach chiet ADN clla Shigella va EIEC
tr~(c tiep tv phan da aUQc phat tri~n thanh congo Ket qua ella nghien cC(u oa dong gop vao vi~c ch~n doan sam va hi~lI qua b~nh tieu chay.
TAl lI~U THAM KHAO
1, Begum D, and M.P. Jackson. Direct detection of Shiga - like toxin - producing Escherichia tion of Shiga - like toxin - producing Escherichia col i in ground beef using the polymerase chain reaction. Mol Cell Probes, 1995.9(4): p. 259 - 64.
2. Beutin L., S. Zimmermann, and /K. Gleier. Rapid detection and isolation of shiga - like toxin Rapid detection and isolation of shiga - like toxin (verocytotoxin) - producing Escherichia coli by direct testing of individual enterohemolytic colo nies from washed sheep blood agar plates in the VTEC - RPLA assay. J Clin Microbiol, 1996. 34 (11): p. 281 2 - 4.
3. Nguyen T.V., et al. Detection and
characterization of diarrhecigenic Escherichia coli
from young children in Hanoi, Vietnam. J Clin Microbiol, 2005.43(2): p. 755 - 60 .
4. Paton A. W. and J.e. Paton. Direct detection and characterization of Shiga toxigenic detection and characterization of Shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA, and saa. J Clin Microbiol, 2002. 40(1): p. 271 -4.
5. Paton, A.W. and J.e. Paton. Direct detection of Shiga toxigenic Escherichia coli detection of Shiga toxigenic Escherichia coli strains belonging to serogroups 0111, 0157, and 0113 by multiplex PCR. J Clin Microbiol, 1999.
37(10): p. 3362 - 5.
6. Ramotar K., et al. Direct detection of verotoxin - producing Escherichia coli in stool verotoxin - producing Escherichia coli in stool
samples by PCR. J Clin Microbiol, 1995. 33(3): p. 519 - 24.
7. Sethabutr 0., et aI., Detection of PCR products of the ipaH gene from Shigella and products of the ipaH gene from Shigella and enteroinvasive Escherichia coli by enzyme linked 108
TCNCYH 55 (3) -2008
::'I-;:~_ immunosor~ent assay. Diagn Microbial Infect Dis, genic Escherichia coli DNA during course of
;' ;. 2000.37(1). p. 11 - 6 infection. JClin Microbiol, 199.').33 (.')): p. 10.')4 - 9. . ~"
. "{:.
·r 8. Stacy - Phipps,S., J.J. Mecca, and J.B.
Weiss. Multiplex PCR assay and simple prepara
Nghien cuu dlrqc thl/c hi~n t~i BQ mon Vi sinh y hQc, D9i hQc Y H3 NQi ttl thang 7 de'n
tion method for stool specimens detect enterotoxi thang 6 nJm 2007. Cd quan tai trq: BQ Y te'
Summary
DEVELOPMENT OF A METHOD fOR DIRECT DNA EXTRACTION Of SHIGEllA AND EIEC fROM fECAL SAMPLE
A method for direct DNA extraction which is simple, fast and efficient is very important in rapid
i
I diagnosis of bacterial pathogens from fecal samples. Objective: development of a method for direct
I extraction of shigella and EIEC DNA from fecal sample. Methods: EIEC ATCC 43893, E. coli ATCC