- Khong co dap Un gm iln djch di truyen
2. PHl/ONG PHAP CHAN DOAN BENH DON BAO K* SINH
2.3. Phuang phap kiem tra mau
Kiem tra mau nham phat hien cac ky sinh trung dudng mau nhu: Trypanosoma sp., Piroplasma sp. (Babesia sp.).
* Lay mau de kiem tra
Ddi vdi gia sue (trau, bd, dd, ngua, 1cm), dung keo vo trung cat nhe d dau tai hoac dung kim tiem vd trung choc ngang mot mach mau nho d dau tai sau khi da cat long va sat trung. Ddi vdi nhung dong vat thi nghiem (chudt bach, chuot lang) thi co dinh dau va nam dudi keo manh roi cat chop dudi. Ddi vdi gia cam va chim thi dung kim dam vao tinh mach canh de lay mau.
* Kiem tra mau tuoi
Cho mot giot mau vao giua phien kmh kho, trong va sach, dat mot la kinh len giot mau sao cho giot mau dan mdng ra (cd the nho vao canh la kinh mdt giot dung dich natri citrat 2% de mau cham ddng, dd kiem tra hon). Kiem tra dudi kinh hien vi. do phong dai 10 x 20 hoac 10 x 40.
Phuang phap kidm tra mau tuai phat hien dupe cac loai tidn mao triing cdn song
(Trypanosoma sp.).
Phuang phap kiem tra tieu ban mau nhuom mau la phuong phap thuang dung de phat hien hau het cac loai ky sinh trung ky sinh trong mau (ke ca cac ky sinh trung ky sinh trong huyet tuong va hong cau).
Ky thuat dan mau tren phien kinh: chuan bi nhung phien kinh mai da dugc tay ma bang cach da dugc rira xa phong that sach, ngam vao con 96°, trudc khi dung l^u kho bang khan mem va khong co xa. Chon nhung la kinh kich thudc 2 x 2 cm, cang mong cang tot, ria that phang va nhan. Dat mot giot mau nho len mot dau phien kinh, cach ba phien kinh khoang 1cm. Dat canh cua mot la kinh len giot mau, nghieng 45° vai phien kinh. Khi giot mau da tran ra khap canh cua la kinh thi day la kinh ve phia truac, lam cho mau dugc dan thanh mot lap mong va deu tren phien kinh (mau dan theo la kinh chu khong phai bi la kinh day di). Co dinh tieu ban bang con methanol. De kho tieu ban roi nhuom Giemsa tir 30 - 60 phut (dung dich Giemsa dugc pha theo ty le: dung dich Giemsa ca ban 1 phan, nude cat trung tinh 9 phan). Rira tieu ban duoi voi nude chay nhe, vay va de kho.
Kiem tra tieu ban dudi kinh hien vi bang vat kinh dau, do phong dai 10 x 90 hoac 10 x 100 (co dau Cedre - dau bach duang).
Phuang phap nay phat hien dugc cac ky sinh triing duang mau: Trypanosoma sp., Theileria sp., Babesia sp. (Piroplasma sp., Anaplasma sp.)...