... times, and key words and phrases, incidents and facts in the text were noted Primary codes were extracted The codes and data were compared for similarities and differences Categories and sub-categories ... study conception and design, data collection, analysis, revision, editing and manuscript writing HK was involved in the conception and design of study and took an active part in the data analysis ... R: Civil society and the state: Turkey after the earthquake Disasters 2002, 26:120-39 30 Liang NJ, Shih YT, Shih FY, Wu HM, Wang HJ, Shi SF, Liu MY, Wang BB: Disaster epidemiology and medical response...
Ngày tải lên: 25/10/2012, 09:56
... descriptive analysis, which included the frequency and type of emergency The frequency of aircraft diversion was also investigated All data regarding names, ages and the sex of the patient and the name ... physiological and psychological stressor for many individuals, potentially triggering a variety of medical emergencies that may occur on board This can lead to challenging situations for physicians ... Germany) and the European Joint Aviation Authorities (JAA; Cologne, Germany) regulate aviation on the national and continental level They regulate by law the contents of an on-board dispensary and...
Ngày tải lên: 25/10/2012, 10:31
Báo cáo y học: " Derivation and preliminary validation of an administrative claims-based algorithm for the effectiveness of medications for rheumatoid arthritis"
... activity As part of a sensitivity analysis, we restricted the cohort to patients without any of these ICD-9 codes as part of a sensitivity analysis As part of two additional sensitivity analyses, ... potentially limited generalizability of patterns of care in the VHA system and possible dissimilarity in the RA patients it treats compared to other RA populations However, sensitivity and specificity, ... biologic therapy [30] As part of a sensitivity analysis, we modified our gold standard to require patients only to achieve LDA (DAS28 < 3.2) and did not include patients who only achieved some...
Ngày tải lên: 25/10/2012, 10:45
Báo cáo y học: "Aplasia and Agenesis of the Frontal Sinus in Turkish Individuals: A Retrospective Study Using Dental Volumetric Tomograph"
... Passageways and Olfactory Organs in Man Philadelphia: P Blakiston’s Son; 1920 Som PM, cCurtin HD Head and neck imaging In: Som PM, Shugar JMA, Brandwein MS, eds Anatomy and Physiology, 4th ed ... Computed Tomography Findings in Chronic Rhinosinusitis Patients with and without Allergy Asian Pac J Allergy Immunol 2006;24:123-7 Schaeffer J The Embryology, Development and Anatomy of the Nose, ... structures, and to lighten the skull and facial bones [8] The exact drainage system of the frontal sinus depends on its embryologic development [2,13] The drainage usually occurs directly into the...
Ngày tải lên: 25/10/2012, 11:04
Báo cáo y học: " Safety and efficacy of undenatured type II collagen in the treatment of osteoarthritis of the knee: a clinical trial"
... was assessed by capsule count at visits 3, 4, and and review of subject diary Efficacy and Safety Variables Efficacy and safety measurements assessed Adverse events During the study, subjects recorded ... analyzed by MDS Laboratory Services (London, Ontario, Canada) Appropriateness of Measurements The efficacy and safety assessments used in this study were standard for OA and are widely used and ... therapy, joint protection and physiotherapy/occupational therapy agrees to continue these therapies as normal avoiding changes in frequency or intensity and to record therapies in the study diary...
Ngày tải lên: 26/10/2012, 09:48
Báo cáo y học: "Epidemiology and Prevention of Hepatitis B Virus Infection"
... data] Genotypes A and D are also found in other areas of China However, the genotypes E-H have not been reported in China Recently, genotype C/D hybrid was identified in Tibet [33] and genotype B ... Norder H Subtypes, genotypes and molecular epidemiology of the hepatitis B virus as reflected by sequence variability of the S-gene Intervirology 1995;38:24-34 53 Buckwold VE, Xu Z, Chen M, Yen TS, ... or hemodialysis, physicians, dentists, nurses and other healthcare workers, laboratory technicians, intravenous drug users, police, firemen, laundry workers and others who are likely to come into...
Ngày tải lên: 02/11/2012, 11:12
Báo cáo y học: "Risk and Benefit of Drug Use During Pregnancy"
... during pregnancy and Poland sequence: Results of a population-based registry and a case-control registry Teratology 1999 59: 35-38 Hwang SJ, Beaty TH, Panny SR et al Association study of transforming ... women using necessary drug treatments may suffer permanent psychological stress and may be seriously depressed until the end of the pregnancy [44] Fortunately the scientifically proved human teratogenic ... pregnancy, at least in “the first trimester” This unbalanced opinion is amplified by a self defensive attitude of some doctors and is partly understandable They may not be well informed and may exaggerate...
Ngày tải lên: 02/11/2012, 11:12
Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"
... centrifuged gently, and the lysate was removed from the beads 100 and placed into a new, siliconized microfuge tube PKC substrate antibody was added at a ratio of 1:200, and the lysate and antibody were ... (Beverly, MA) Claudin-1 antibody (1:1000) was obtained from Zymed Laboratories (San Francisco, CA) Cells were grown to 80% confluency and then harvested on ice as previously described (4) Nuclear and ... immunoprecipitated by the PKC substrate antibody G361 cells have very little claudin-1 and thus, none is precipitated by the PKC substrate antibody, indicating the specificity of this immunoprecipitation...
Ngày tải lên: 03/11/2012, 11:17
Báo cáo y học: " Induction and effector phase of allergic lung inflammation is independent of CCL21/CCL19 and LT-beta
... confirmed by real-time PCR analysis of RNA for IL-2, IL-4, IFNγ, and HPRT, and calculating the IL-4/IFNγ ratios Intranasal challenge was performed on day 14, 15 and 16 (days 0, 1, and after transfer) ... PA) Eosinophils, monocytes/macrophages and lymphocytes were enumerated based on morphology and staining and expressed as percentages of total BAL cells [28,29] Histology Frozen lung sections ... airway hyperreactivity but cause severe airway inflammation J Clin Invest 1999; 103: 175-83 [32] Cohn L, Tepper JS, Bottomly K IL-4-independent induction of airway hyperresponsiveness by Th2,...
Ngày tải lên: 03/11/2012, 11:24
Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf
... one slowly by Dnmt3a or by de novo methylation catalyzed by Dnmt1 and the second one very fast Therefore, this effect can only double the rate of DNA methylation, because every turnover by Dnmt3a ... DNA methylation by cooperation of Dnmt3a and Dnmt1 A 958mer PCR product (20 nM) was methylated using labeled [methyl-3H]-AdoMet by Dnmt1 (80 nM) (j), Dnmt3a (400 nM) (d) and both enzymes present ... methylation of DNA that postulates functional cooperation of Dnmt1 and Dnmt3a Purified Dnmt1 and Dnmt3a enzymes were employed to methylate a 958mer PCR product using labeled [methyl-3H]-S-adenosylmethionine...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: DNA and RNA damage by Cu(II)-amikacin complex docx
... 119, 617– 618 34 Yanagawa, H., Ogawa, Y & Ueno, M (1992) Redox ribonucleosides Isolation and characterization of 5-ydroxyuridine, 8-hydroxyguanosine & 8-hydroxyadenosine from Torula yeast RNA J Biol ... Ami and Cu(II)-Ami complex induced highly specific cleavage in the anticodon loop, at the hipermodified base Y3 7 (wybutine) (Figs and 7) Also other aminoglycosides, as kanamycin A, neomycin B and ... results of the strand-break assay of yeast tRNAPhe in the presence of Ami, Cu(II)-Ami and the Cu(II)-Ami-H2O2 system, performed by means of polyacrylamide gel electrophoresis Strikingly, in the presence...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Human and Drosophila UDP-galactose transporters transport UDP-N-acetylgalactosamine in addition to UDP-galactose doc
... numbers O76865 and O9W4W6) and indicated by the arrowheads The symbol ÔVÕ indicates a potential Nglycosylation site A putative polyadenylation signal is enclosed by a box (B) Hydrophobicity plot of ... mM phenylmethanesulfonyl¯uoride], and the samples were fractionated by electrophoresis on a 12% SDS/polyacrylamide gel The separated polypeptides were electotransferred to a poly(vinylydene di¯uoride) ... pYEX-BESN-transformed YPH500, pYEX-BESN-DmNST-cHA-transformed YPH500, and pYEX-hUGT-cHAtransformed YPH500 Microsomal vesicles (50 lg protein/assay, or in GDP-Man transport assay, 30 lg protein/assay) were incubated...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: Antibacterial and antifungal properties of a-helical, cationic peptides in the venom of scorpions from southern Africa pptx
... spiders and insects Parabutoporin and opistoporin were synthesized chemically and preliminary studies on antibacterial activity showed that the quality and biological activity of native and synthesized ... at 37 °C Controls for zero and 100% hemolysis were determined by buffer and distilled water, respectively moment, hydrophobicity and angle subtended by the positively charged residues in a helical ... Hemolytic activity is influenced more by hydrophobic than by electrostatic interactions, but the most hemolytic peptide in our study is melittin, although mastoparan has a higher hydrophobicity Hydrophobicity...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... the N-acetyl group is stabilized by hydrogen bonds between its carbonyl oxygen atom and the Tyr183 hydroxyl group, and between its amide nitrogen atom and Asp125 In the mutants, the hydrogen bond ... the transition state by hydrogen bonding to the )1 N-acetyl carbonyl oxygen atom This hydrogen bond stabilizes the partially negative charge on the carbonyl oxygen, thereby facilitating Ó FEBS ... (1995) Stereochemistry of chitin hydrolysis by a plant chitinase/lysozyme and X-ray structure of a complex with allosamidin: evidence for substrate assisted catalysis Biochemistry 34, 15619–15623...
Ngày tải lên: 22/02/2014, 04:20
Tài liệu Báo cáo Y học: Structural and biochemical characterization of neuronal calretinin domain I– II (residues 1– 100) Comparison to homologous calbindin D28k domain I–II (residues 1 –93) pdf
... Extracellularly expressed CR I–II using Pichia pastoris was purified by DEAE chromatography [32] and the proteins eluted were desalted by dialysis The resulting protein solutions were lyophilized to dryness ... apo- and calcium-bound domains are essential to understand fully the differences in CR I–II and Calb I–II structure and properties Are the functions of CR and Calb distinct? A large body of physiological ... stereochemical investigation of the role of lysine-32 in the phenylpyruvate tautomerase activity catalyzed by macrophage migration inhibitory factor Biochemistry 38, 16024–16033 44 Morris, G & Barjat,...
Ngày tải lên: 22/02/2014, 07:20
Tài liệu Báo cáo Y học: Characterization and regulation of yeast Ca2+-dependent phosphatidylethanolamine-phospholipase D activity docx
... 1-Acyl-2-[6-N-(7-nitrobenzo-2-O-1,3-diazol-4-yl)-amino]caproyl-glycero-3-phosphorylcholine (C6-NBD-PtdCho) and 1-acyl-2-[6-N-(7-nitrobenzo-2-O-1,3-diazol-4-yl)-amino]-caproyl-glycero-3-phosphorylethanolamine ... extract and Bactopeptone (YP) containing 2% dextrose]; YPA (YP containing 0.05% glucose and 2% potassium acetate); and YPG (YP containing 3.5% galactose) Phospholipase D assays Spo14p/Pld1p and PtdEtn-PLD ... lysis on membrane and cytosolic PtdEtn-PLD activity Yeast cells were lysed in the presence of EGTA (1 mM; left) or CaCl2 (10 mM; right) and the membrane and cytosol fractions were separated by...
Ngày tải lên: 22/02/2014, 07:20
Tài liệu Báo cáo Y học: Presence and regulation of the endocannabinoid system in human dendritic cells potx
... Widen, R & Friedman, H (1985) The effect of delta-9-hydrocannabinol and 11-hydroxy-delta-9-tetrahydrocannabinol on T-lymphocyte and B-lymphocyte mitogen response J Immunopharmacol 7, 451–466 Klein, ... antibody at a dilution of : 400 for CB1 polyclonal antibody, : 250 for CB2 polyclonal antibody, and : 200 for FAAH polyclonal antibody A control was made in the same conditions using the CB1 polyclonal ... Biosynthesis, uptake, and degradation of anandamide and palmitoylethanolamide in leukocytes J Biol Chem 272, 3315–3323 Pestonjamasp, V.K & Burstein, S.H (1998) Anandamide synthesis is induced by...
Ngày tải lên: 22/02/2014, 07:20
Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver potx
... Long Template PCR System, Staphylococcus aureus V8 protease, N-[N-(L-3-trans-carboxyrane-2-carbonyl)-L-leucyl]-agmantine (E-64), leupeptine, phenylmethylsulfonyl ¯uoride (PMSF), and thesit were ... were examined by ¯uorescence microscopy and the images were acquired by using a Spot-2 cooled camera (Diagnostic Instruments) Sterol D14-reductase assay D14-SR activity was assayed in microsomes ... Biochim Biophys Acta 1437, 80±92 26 Cleveland, D.W., Fischer, S.G., Kirschner, M.W & Laemmli, U.K (1977) Peptide mapping by limited proteolysis in sodium dodecylsulfate and analysis by gel electrophoresis...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc
... other 2Cys-Prx enzymes previously described in yeast, mammals and plants, Ch-Prx1 displayed antioxidant and peroxidase activities The enzyme could reduce hydrogen peroxide as well as alkyl hydrogen ... puri®ed by using the nucleospin extract kit (Macherey±Nagel), then digested by NcoI and BamHI, and cloned into the pET-8c vector Screening of a cDNA library A kgt11 cDNA library of Chlamydomonas ... extract and extensive washing, was a major protein of 21 kDa (Fig 1) This protein was further puri®ed to homogeneity by HPLC and digested with trypsin The tryptic peptides were separated by HPLC and...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase ppt
... dried by RAPIDRY gel-dry system (ATTO, Tokyo, Japan) for 90 at 80 C and used for autoradiography ATP and ADP were separated and detected by thin layer chromatography (TLC) with a previously described ... onto lanes and 2, respectively The subunits AclA and AclB of wild type and H273A Cl-ACL were dissociated by hydroxyapatite column chromatography AclB eluted with 10100 mM KPB (lanes and 6), while ... phosphorylated carbonyl carbon of citryl phosphate, and the cleavage of the resulting citryl-CoA to acetyl-CoA and oxaloacetate In our previous report, we could not detect citrate synthase activity...
Ngày tải lên: 08/03/2014, 22:20