... the low pressure (Fig 2A) and high pressure (Fig 2B) forms of apo-, Mg2+and Mn2+-enolase The low pressure form is fully dimeric; based on previous experiments, we assume that the high pressure ... and high pressures The values of D2 were used to calculate Kd for dissociation of the dimer into monomers, assuming that the low pressure value of D2 is that of dimeric enzyme and the high pressure ... identical spectra at low pressure, they not have the same high pressure spectra; this is most apparent for the parameter, D1 With the Mn2+ enzyme, D1 decreases very slightly at high pressure; with the...
Ngày tải lên: 19/02/2014, 16:20
... rich chemistry, temperature and high pressure prevailing around the deep-sea vents offer a plausible environment for the emergence of life [35] High hydrostatic pressures (up to 200 MPa) were ... the hairpin ribozyme subjected to high hydrostatic pressures is completely recovered upon returning to atmospheric pressure, indicating that the effects of pressure are fully reversible, as was ... steady state studies of enzymatic reactions at high pressure: tests with aspartate transcarbamylase Anal Biochem 187, 258–261 51 Heremans K (1982) High pressure effects on proteins and other biomolecules...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: High-pressure effects on horse heart metmyoglobin studied by small-angle neutron scattering pdf
... 000 g during at 15 C prior to the SANS experiments High- pressure cell During the SANS experiments the protein solutions were contained in a high- pressure cell made of stainless steel with two parallel ... function of pressure, P moderate pressures near 300 MPa induce subtle structural rearrangements of the protein matrix whereas higher pressures, near GPa, lead to unfolding Pressure may also induce changes ... Heremans, K., Frank, J., Masson, P & Balny, C (1996) High- pressure eects on protein structure and function Proteins 24, 8191 Heremans, K (1982) High- pressure eects on proteins and other biomolecules...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo Y học: Substrates modulate the rate-determining step for CO binding in cytochrome P450cam (CYP101) A high-pressure stopped-flow study pdf
... solution at the beginning indicating that P420 was not formed during the high- pressure stopped-flow experiment The high- pressure stopped-flow apparatus used is interfaced with the Aminco DW2 spectrometer ... (see below) Ó FEBS 2002 High- pressure stopped-flow for P450 CO binding (Eur J Biochem 269) 2991 Three shots at each pressure were taken and fitted The kon values for the same pressure were averaged ... 20 21 22 23 24 25 26 27 28 hemeproteins High- pressure study J Am Chem Soc 112, 6880– 6886 Unno, M., Ishimori, K., Ishimura, Y & Morishima, I (1994) High- pressure flash photolysis study of heme...
Ngày tải lên: 08/03/2014, 23:20
How and Why does the Efficiency of Regional Innovation Systems Differ? doc
... an easily accessible location characterized by relatively high density of population and economic activity A center has a relatively high rank in the spatial hierarchy In contrast, regions in ... location Based on these arguments, one may expect innovation activities to operate at a higher level and with higher productivity at the center as compared to the periphery Therefore, a certain degree ... case studies suggest that spatial clustering or density of innovation activities does not necessarily lead to a higher level of cooperation between the firms or research institutions in a particular...
Ngày tải lên: 16/03/2014, 03:20
WHY DOES FEMINISM MATTER TO AESTHETICS? potx
... puzzles in aesthetics from a feminist perspective; this answer does not so much alleviate as exacerbate my concern I began by wondering why feminism was not included in Lamarque’s essay given that ... to The British Journal of Aesthetics The provocative question this lack raises is why there have been so few Why feminists find it difficult to see their work as suitably 'philosophic' to submit ... reasons First, her essay does not make heavy use of technical terminology popular in contemporary continental philosophy She makes passing reference to Heidegger, but she does rely on more recent...
Ngày tải lên: 16/03/2014, 18:20
WHY DOES THE WORLD STAY GREEN? Nutrition and survival of plant-eaters doc
... constantly striving to get enough of them As a result they can produce few viable young, Why does the world stay green? and most of those they produce soon starve And they will die whether or not others ... the key 88 Chapter Plagues, outbreaks and the tyranny of weather Weather’s dramatic effects 93 93 Successful reproductive strategies 100 Weather can affect food quality 104 Afterword 111 Further ... enable them to photosynthesise Why then does the combined impact of all the many animals that feed on plants not make any impression on their number and volume? Why is it that, with the exceptions...
Ngày tải lên: 22/03/2014, 19:20
Does High Public Debt Consistently Stife Economic Growth? A Critique of Reinhart and Rogoff pptx
... country-years in the highest public debt/GDP category) has a major effect on the computed average in the highest public debt/GDP category It reduces the average growth for New Zealand in the highest public ... real growth in the high public debt category is +2.2 percent per year compared to the −0.1 percent per year published in RR The actual growth gap between the highest and next highest debt/GDP categories ... the highest public debt/GDP category with only 71 country-years of data: 25 years of Belgium were dropped in addition to the 14 already All of these cases would contribute observations to the highest...
Ngày tải lên: 23/03/2014, 20:20
Báo cáo Y học: Fluorescence study of the high pressure-induced denaturation of skeletal muscle actin pdf
... ATP-G-actin [20] High pressure apparatus High pressure devices used for this study consisted of a thermostated high pressure vessel equipped with sapphire windows and a pump capable of raising pressure ... then reached a plateau On a further increase in pressure, it decreased gradually in a relatively lower pressure range and steeply in a higher pressure range At 400 MPa it dropped almost to the ... function of pressure time at various pressures The solid lines show the best curve ®t of a ®rst order kinetics The experimental conditions were the same as in Fig The to represent the pressure intensities...
Ngày tải lên: 24/03/2014, 00:21
Báo cáo Y học: High pressure-induced changes of biological membrane Study on the membrane-bound Na+/K+-ATPase as a model system pdf
... 220 MPa or higher It was con®rmed that the excitation spectra of BIPM and FITC not change under these high pressures Hydrogen±tritium exchange under high pressure Effects of high pressure on ... The buffer used in the high pressure experiments were 40 mM Tris/HCl, pH 7.4, to minimize the pressure- induced pH changes [25] In situ spectroscopic measurements under high pressure Absorbance and ... increasing pressures are shown in Fig 2A The value for kmax was shifted from 341 to 345 nm with increasing pressure, up to 220 MPa, and then decreased for pressures of 220 MPa or higher, being...
Ngày tải lên: 24/03/2014, 00:21
Báo cáo khoa học: Charging of tRNA with non-natural amino acids at high pressure potx
... we used a high- pressure technique High pressure is currently used in many areas of biotechnology Its mechanism of action includes the decisive role of water structure [26,27] High pressure allows ... aminoacylation reaction of tRNA at high pressure remains unknown Recently, we obtained new information about the conformation of tRNA at elevated pressure [26] It is known that high pressure lowers the pH ... nucleophilic attack (Fig 10) The entire process is induced by high pressure and does not proceed without it We showed that high pressure influences the conformation of tRNA because of rearrangements...
Ngày tải lên: 30/03/2014, 10:20
báo cáo hóa học:" Modified femoral pressuriser generates a longer lasting high pressure during cement pressurisation" pot
... controlled and reduced cement leakage the pressure drops more gradually allowing the high pressure to be retained for longer The time with high cementation pressure (250 kPa) was about twice that ... conventional pressuriser tested here can reach a high pressure, nearly 300 kPa (2256 mmHg), but due to cement leakage did not retain this pressure The changes of pressures depend of how the pressurisers ... cement pressurisation lead to increased cement penetration [6,7,11-16] while constant high pressure benefits high viscosity cement penetration whether in a constrained cavity such as the femur...
Ngày tải lên: 20/06/2014, 07:20
Báo cáo hóa học: " Landmine Detection and Discrimination Using High-Pressure Waterjets" potx
... signal produced by the impingent waterjet is characteristically different for different types or classes of objects [9, 10] Our objective is to show the potential of using the sound produced by a high- pressure ... CONCLUSIONS The goal of this study was to show the potential of using the sound produced by the impact of a high- pressure waterjet to detect and classify buried landmines Previous work had shown ... to set off a landmine If needed, even less force can be used by decreasing the pressure or nozzle size Depending on pressure, nozzle diameter and firing time, the waterjet can penetrate up to 12...
Ngày tải lên: 23/06/2014, 01:20
Báo cáo khoa học: "Hydraulic conductance of root and shoot measured with the transient and dynamic modes of the high-pressure flowmete" ppsx
... pressurisation of root systems [6], the root pressure probe [18], the potometer [29], the negative pressure flow system for root sections [14], the high pressure flowmeter (HPFM) [25, 27] Each method ... recording flow and pressure; k is calculated from the linear regression slope of flow versus applied pressure It should be noticed that xylem vessels are refilled by the high pressure water perfusion ... the high pressure flow meter in crop plants, J Exp Bot 51 (2000) 823–828 [25] Tyree M.T., Patino S., Bennink J., Alexander J., Dynamic measurements of root hydraulic conductance using a highpressure...
Ngày tải lên: 08/08/2014, 14:21
Báo cáo khoa học: "Whole shoot hydraulic resistance in Quercus species measured with a new high-pressure flowmeter" pps
... to the highpressure flowmeter described below, all cut surfaces were shaved with a razor blade to remove blockage of cut vessels by cell-wall fragments formed by the initial cuts The high- pressure ... a differential pressure gauge like that used in a lowpressure flow meter described by Tyree (1983) However, that would have eliminated the main advantages of the present high- pressure flow meter, ... a pressure reservoir (R) Water contained in the reservoir was distilled water filtered through an 0.1 μm filter The water was placed under pressure by compressed air, controlled with a pressure...
Ngày tải lên: 08/08/2014, 23:22
Báo cáo y học: " What is MRI bone oedema in rheumatoid arthritis and why does it matte" ppt
... examined TNF-transgenic mice using a high- resolution Tesla MRI scanner They described the presence of bone oedema and correlated this histologically with a highly cellular infiltrate within the ... oedema with current theories of the immunopathogenesis of RA Hirohata and colleagues [23], in a highly accessed article published in Arthritis Research and Therapy in early 2006, described a study ... cells that were abnormally sensitive to tumor necrosis factor (TNF)α [24] were found to express high levels of the nuclear factor kappa B (NFκB) transcription factor, contrasting with cells from...
Ngày tải lên: 09/08/2014, 08:23
Báo cáo y học: "Revisiting the pathogenesis of podagra: why does gout target the foo" docx
... slower walking velocity, reduced step and stride length, reduced peak plantar pressure under the hallux, and higher mid-foot pressure- time integrals compared to controls The authors postulate that ... REVIEW JOURNAL OF FOOT AND ANKLE RESEARCH Open Access Revisiting the pathogenesis of podagra: why does gout target the foot? Edward Roddy Abstract This invited paper provides a summary of a keynote ... http://www.jfootankleres.com/content/4/1/13 Firstly, does an association exist between the disease states of gout and nodal generalised OA? These two conditions share the common risk factor of obesity...
Ngày tải lên: 10/08/2014, 21:24
Báo cáo khoa học: "What does high NT-proBNP mean in septic shock patients? A part of the puzzle" pdf
... NT-proBNP level with cardiac dysfunction In a seminal paper, Charpentier and coworkers [5] reported higher BNP levels in septic patients with altered left ejection fraction In the study by Mokart ... upregulation of BNP and NT-proBNP secretion, whereas cardiac dysfunction could contribute to persistent high NTproBNP levels A limitation of the study by Mokart and coworkers is the lack of serial measurements...
Ngày tải lên: 13/08/2014, 03:20
ASTM D129 Standard Test Method for Sulfur in Petroleum Products (General High Pressure Decomposition Device Method)
Ngày tải lên: 20/10/2014, 17:16
Báo cáo y học: " High blood pressure, antihypertensive medication and lung function in a general adult population"
... antihypertensive medication irrespective of high blood pressure Furthermore, an effective blood pressure treatment and an ineffective blood pressure treatment, meaning high blood pressure despite the use of ... high blood pressure Furthermore, the multivariable regression analysis revealed a significant interaction between gender and high blood pressure This might possibly indicate that high blood pressure ... medical treatment have a higher underlying blood pressure compared to effectively treated subject Moreover, the effects of high blood pressure and antihypertensive medication are highly correlated A...
Ngày tải lên: 25/10/2012, 10:45
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