whose molecular function is still undefined

... Identifica- tion of a new isoform of the human estrogen receptor- alpha (hER-alpha) that is encoded by distinct tran- scripts and that is able to repress hER-alpha activation function 1. EMBO J 19, ... signals were seen in other tis- sues, including testis (Fig. 2A). In comparison, very low levels of the AR transcript were detected in heart, as compared to liver or testis (Fig. 2B). The transcript levels ... fluorescent brain brain testis testis liver liver prostate prostate lung lung trachea trachea muscle muscle breast breast heart heart kidney kidney uterus brain testis liver prostate lung trachea muscle breast heart kidney uterus uterus AR45 S9 A B C AR Fig. 2. Analysis of tissue distribution of AR45 by RT-PCR analysis of human RNA. First-strand cDNA from the indicated organs was used as...

Ngày tải lên: 07/03/2014, 16:20

Ngày tải lên: 18/06/2014, 16:20

Ngày tải lên: 20/06/2014, 08:20

... field? Distribution of molecules is given by distribution functions. We will consider two such distribution functions: • Distribution on the height (or potential energy) in a gravitational field • Distribution ... : The function P(v) = gives the Maxwell-Boltzmann distribution function of molecular speeds. Remark that the Maxwell-Boltzmann distribution function depends on temperature. This dependence is shown ... C V = 3R  The Maxwell-Boltzmann distribution function of molecular speeds P(v) = 4/22/2008 19 The function is known as the Maxwell-Boltzmann velocity distribution function. In a velocity diagram,...

Ngày tải lên: 17/01/2014, 04:20

... alone is delivered to the vacuole by endocytosis, where it is hydrolyzed by the resident Ath1. However, this model requires the identification of a trehalose endocytosis process and this is difficult ... that trehalase in the vacuole is likely prompted to partial degradation and is not required for cell growth on trehalose. The TM domain is indispensable for Ath1 function Previous studies have ... Arabidop- sis. Plant Physiol 125, 1086–1093. 30 Frison M, Parrou JL, Guillaumot D, Masquelier D, Francois J, Chaumont F & Batoko H (2007) The Ara- bidopsis thaliana trehalase is a plasma...

Ngày tải lên: 18/02/2014, 06:20

... TM sequence. Consistent with this, our analysis of cellular membranes washed with high-salt solution establishes that RNF13 is indeed an integral membrane protein. This is consistent with data ... that it is local- ized to the cytosol (data not shown). RNF13 expression is higher in adult than in embryonic tissues Genome sequencing suggests that RNF13 is ubiqui- tously expressed. This is supported ... it has a TM domain. A recent study established that RNF13 is an E3 ubiquitin ligase whose expression is increased in pancreatic ductal ade- nocarcinoma tissues, suggesting that the protein may participate...

Ngày tải lên: 18/02/2014, 08:20

... gene expression, and protein function. A particular aspect that is special to the BioMillenium series of conferences (this has been the 10th in this series) is the emphasis on new and emerging technologies; ... cell masses. Pericellular proteolysis is hence a prerequisite for expansive tumor growth, collective invasion and large-scale tissue reshaping. In contrast, single-cell dis- semination associated with ... interferon, but the exchange rate in unstimulated cells is more than 10 times lower. In this context Georgyi Los (Madison, WI, USA) and Marjeta Urh (Madison, WI, USA) described the use of a fusion between...

Ngày tải lên: 19/02/2014, 02:20

... characteristic of this kinase (Fig. 4D) [28]. Comparison of the expression profiles of the human ER and POLDIP3 genes The array of polyA + RNA isolated from 61 different human adult tissues, seven ... conferring resistance to zeocin was transformed into the yeast strain L40 [MATa his3D200 trp1-901 leu2-3112 ade2 LYS2::(4- lexAop-HIS3) URA3::(8lexAop-lacZ) GAL4]. Five independ- ent zeocine-resistant ... gene has been iden- tified in eukaryotic organisms ranging from protists to plants to humans with the exception of fungi, in which this gene has not been isolated so far [1–5]. Moreover, it seems...

Ngày tải lên: 19/02/2014, 05:20

... target genes whose transcription is STAT2-dependent but ISGF3-independent Melissa M. Brierley 1 , Katie L. Marchington 1 , Igor Jurisica 2 and Eleanor N. Fish 1 1 Department of Cell and Molecular ... the existence of a collection of GAS-regulated target genes whose expression is IFN-inducible and independent of ISGF3 but highly dependent on the STAT2 DNA binding domain. This report is the ... genes. The c-fos gene was examined in this system as an example of a GAS-driven gene whose IFN-inducibility is independent of both ISRE and STAT2. Our analysis confirmed previous findings [21] that...

Ngày tải lên: 19/02/2014, 07:20

... this panel, which is supposed to debate the necessity of continuing work in this area rather than to debate technical issues in the area. And yet if this problem has been solved, where is ... male or female". This same system, when asked for all the Michigan doctors and Pennsylvania dentists, produced a list of all the people who were either doctors or dentists and who lived in ... who lived in either Michigan or Pennsylvania. This is the state of our art? Instead of assuming that the problem is one of using typed English to access and/or update a file or files in a...

Ngày tải lên: 21/02/2014, 20:20

... nucleus of embryonic cells during the progression of embryogenesis. This finding is consistent with nTG being involved in the histone dimerization reaction. We have shown that the treatment of ... Ikemagi, unpublished results). The acetylation of Lys5 of histone H4 competes with the TG-catalyzed histone dimerization reaction because a n acetylated lysine re sidue is not a functional amine ... unique property of nTG, not found in other TGs, is that its distribution is confined to the nucleus. Nuclear localization of TG has been reported in the studies on tissue TG [8,9]. A nuclear transport protein,...

Ngày tải lên: 08/03/2014, 10:20

... acquired in the data-depen- dent acquisition analysis or to build an inclusion list with the light and heavy precursor masses for cross-linked candi- date peptides analysis. NanoLC-LTQ-Orbitrap data ... (2004) [URE3] prion propagation is abolished by a mutation of the primary cytosolic Hsp70 of budding yeast. Yeast 21, 107–117. 6 Savistchenko J, Krzewska J, Fay N & Melki R (2008) Molecular chaperones ... Ure2p is at the origin of the [URE3] trait in the baker’s yeast Saccharomyces cerevisiae [1,2]. The propagation of the prion element [URE3] is highly dependent on the expression of a number of molecular...

Ngày tải lên: 15/03/2014, 00:20

... for useful discussions. This work was partially supported by funds from the Spanish Ministry of Education (BIO2007-63458 to MP). J.B. is a recipient of a pre- doctoral fellowship from the Spanish Ministerio ... with eukaryotic hosts. As such, the comparison with BPTP is expected to genuinely display the effects of evolution in lmwPTPs of two phylogenetically distant organisms. The very similar fold of YwlE ... Gaussian distribution. The newly gener- ated relaxation rate data set is fitted to the applied model. The process is repeated 100 times. The final error in the dis- sociation constants is equal...

Ngày tải lên: 16/03/2014, 02:20

... variant displays two free Cys residues (Cys15 and Cys74), whose participation in intermolecular disulphide bonds gives rise to higher-order covalent multimers [11]. Data regarding the molecular ... presented here Cys15 is located on the tip of the N-terminal CCP domain. This latter location is consistent with the low steric hindrance required for the formation of inter-chain disulphide bonds ... quaternary structure consists of a dimer cova- lently linked by a disulphide bond between Cys15 resi- dues of each a-chain. Accordingly, the molecular model of dimeric HPT1 is formed through a tip-to-tip arrangement...

Ngày tải lên: 23/03/2014, 06:20

... subsequently used to isolate membrane fractions. Mutant or wild-type a1 subunits were isolated from this mixture by immunoprecipitation with an anti-a1 immunoglobulin. As shown in Fig. 3, it is apparent Fig. ... subunit is known to influence the interactions of the enzyme w ith K + and because Ser894 is within a sequence known to interact with the b subunits, it is possible that mutations of this amino ... Scheiner-Bobis, G. (2001) A hybrid between Na + ,K + -ATPase and H + ,K + -ATPase is sensitive to palytoxin, ouabain, and SCH 28080. J. Biol. Chem. 276, 2608–2615. 27. Jaisser, F., Canessa, C.M., Horisberger,...

Ngày tải lên: 23/03/2014, 13:20

... proteins. Keywords: GRP94; HtpG; molecular chaperone; dimer; client binding. The 90-kDa heat shock protein (HSP90) is a ubiquitously distributed molecular chaperone and is an essential protein in eukaryotic ... possesses two distinct client- binding sites [14,15]: one of them is located in the N-terminal domain and its activity is modulated by ATP Correspondence to T. K. Nemoto, Division of Oral Molecular Biology, ... charged region is truly indis- pensable for client binding or simply present adjacent to the client-binding site with the result of being affinity-labeled with the client peptide. This issue is now under...

Ngày tải lên: 23/03/2014, 20:22

... not GSt alone, was able to bind cytokeratin 18. Human tissular distribution To examine the human tissue distribution of p76 RBE ,a Multiple Tissue Expression (MTE TM ) Array representing a Fig. ... p76 in the uterus is restricted to the endometrium tissue and is not detectable in myometrium or uterine cervix. DISCUSSION Thyrotropin, via the adenylyl cyclase/cAMP pathway, is the most important ... [23]. This screening was aimed at isolating new proteins whose expression was regulated by the thyrotropin-dependent pathway. Analysis of the amino acid sequence of p76 RBE revealed that it is homologous...

Ngày tải lên: 23/03/2014, 21:20

Ngày tải lên: 30/03/2014, 20:20

Ngày tải lên: 31/03/2014, 08:20

Ngày tải lên: 12/05/2014, 16:08