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Risks Involved in the Use of Herbal Products
... exists in the antioxidant and otherbioactivities of flavonoids, which are powerful in assays conducted in vitro; the 14 Risks Involved in the Use of Herbal Products 353commonly found in over -the- counter ... to inhibit phase I and induce phase II carcinogen metabolizing enzymesthat initiate carcinogenesis. They also inhibit the promotion stage of carcinogen-esis by inhibiting oxygen radical-forming ... consistent in classifying the antioxidant capacity of the polyphenol-rich beverages in descending order: Punica granatum L. (pomegranate) 14 Risks Involved in the Use of Herbal Products 361ephedrine...
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Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc
... model of the a-carbanion/enamine intermediate of the substrate indole-3-pyruvate with ThDP in the active site of IPDC was built based on the three-dimensional structure of the corresponding intermediate ... involving the C-terminal part of the polypeptide chain (Fig. 7). In the latter, differences in the conformation of the loop betweenFig. 6. Stereo picture of the model of the a-carbanion/enamine intermediate ... presence of DNA-bindingproteins involved in regulation of the gene expression of indole-3-pyruvic acid decarboxylase, a key enzyme in indole-3-acetic acidbiosynthesis in Azospirillum lipoferum...
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Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf
... additional genes encoding other proteinsimplicated in the biosynthesis of the aminonucleoside moiety of A201A. Here we report the sequencing of a total of 6946 bp, which include the ata genes most ... atranscription terminator. The resulting fragment onlycontains the initial 67 bp and the final 68 bp of the pur4coding sequence. It was inserted in plasmid pGM9. The resulting construct was introduced, ... shown)[33,34]. In contrast, its similarity to type III PKSs, which lack this domain, is scant. This domain promotes the binding of the acyl-CoA initiation unit to the ketosynthetasedomain of the PKSs...
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Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt
... at the final stage of starch synthesis in the absence of concomitant amylopectin synthesis (reviewed in [41]). In vitrosynthesis of amyloseBecause in Chlamydomonas amylose synthesis remainsactive ... from the results listed in Table 1 that most, if not all, of the amylose issynthesized in vivo when the rate of amylopectin synthesisbecomes minimal. At these moments the rates of amylopec-tin ... rate of amylopectin synthesis. The increase in crystallinity wit-nessed during these first 12 h is in line with the high ratesobserved for amylopectin synthesis. However between 12and 58 h the...
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Báo cáo khoa học: Identification of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt
... catalytic site of GalNAc-T1, might be involved in the catalytic function of the enzyme.To investigate whether the cysteine residues modified byPCMPS are involved in the binding of UDP-GalNAc,we ... mutant proteins was performed asdescribed in Fig. 8. No change in pH was observed during the reaction.This indicates that the inactivation of the mutants was not due toacidification of the incubation ... cysteine residues not involved in the formation of a disulfide bond), by cysteine-specific labeling, to study the mechanistic involvement of the conserved cysteine residues in the function of GalNAc-T1....
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Báo cáo khoa học: Oxidative stress is involved in the permeabilization of the inner membrane of brain mitochondria exposed to hypoxia/reoxygenation and low micromolar Ca2+ Lorenz Schild1 and Georg Reiser2 doc
... degree of protection by ADP sug-gests that the mitochondrial injury is caused by open-ing of the mPTP. There is evidence that ADP inhibitsopening of the mPTP by occupying binding siteslocated in ... consumption was analysed. The corresponding values are shown in Fig. 1. After 15 min of incubation in the presence of 3.5 lm Ca2+ in an air atmosphere, state 3 respiration of brain mitochondria was ... reperfusion in brain.There is a growing body of information concerning the mechanism of ROS generation by the respiratorychain in mitochondria. Complex I and complex IIIwere identified as generators of...
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Báo cáo khoa học: Factors involved in the assembly of a functional molybdopyranopterin center in recombinant Comamonas acidovorans xanthine dehydrogenase pot
... of XDH requires the incorporation of the MPT center, placement of the terminal sulfur ligand on the Mo, and the incorporation of two [2Fe)2S] centers and anFAD into the recombinant enzyme. Therefore, ... of the level of culture aerationon the expression of recombinant protein. Raising the cellular redox potential by increasing aeration levels isexpected to reduce the level of cysteine and increase ... in R. cap-sulatus. The protein product of xdhC appears to exhibit aredox role in maturation of the XDHABCby eliminating the dependence of its functional expression on the redox state of the...
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Báo cáo khoa học: RNA-binding properties of HCF152, an Arabidopsis PPR protein involved in the processing of chloroplast RNA pdf
... in the UV-crosslinkingcompetitive assays, as shown in D. The IC50 of each RNA was determined by plotting the data (D). The location of the high affinitybinding site in the Dd120 RNA defined in ... only one, the atpF intron, while CRS2 is involved in the splicing of nine of the 10 chloroplast group IIB introns [6,15,37]. The expression of CRS2 in E. coli together with the corresponding RNA ... splicing products of the petB intron could be that the protein binds to the UTR region between psbH and petB, and to domain IV of the petB intron. The binding of the homodimer of HCF152 in this region...
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Báo cáo khoa học: The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III fromArabidopsis thaliana pdf
... confirmed the results obtained in the pull-down assays, showing that there is a physicalinteraction between the N- and C-terminal domains of SSIII in vitro.Mapping of the CD-binding region in the N-terminal ... D3 domains,respectively, but not the individual starch-binding domains, are involved in the interaction with the catalytic domain. We also determined that the resi-dues W366 and Y394 in the D2 ... acids of the N-terminalregion responsible for SSIII regulation. We foundevidence indicating that two regions, D(316–344) in the D2 domain and D(495–535) in the D3 domain, are involved in the interaction...
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Báo cáo khoa học: Cell death-inducing DFF45-like effector, a lipid droplet-associated protein, might be involved in the differentiation of human adipocytes pdf
... using quantitative PCR. The resultsrevealed that the mRNA level of adipophilin was not changed, the mRNA levels of perilipin and FABP were decreased and the mRNA level of PPARc was increased in ... subcellular compartments other than lipid droplets. The expression of CIDEC is elevated during the differentiation of adipocytesTo gain further insight into the roles of CIDEC in adipocyte differentiation, ... analyses of the relativelevels of the indicated proteins after wes-tern blotting (as in D) were carried out. Simi-lar experiments were performed five timesand the intensity of the individual...
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Báo cáo khoa học: An E3 ubiquitin ligase, Synoviolin, is involved in the degradation of immature nicastrin, and regulates the production of amyloid b-protein doc
... ⁄ ))]. These results indicatethat a functional deletion of Synoviolin causes a change in the intracellular trafficking of NCT.Effect of Synoviolin on the production of AbNCT is one of the essential ... Synovi-lin-mediated ubiquitination also regulates the traffick-ing of NCT within the Golgi compartment, because the maturation of the sugar modification of the pro-tein occurs within the Golgi ... nicastrin was co-immuno-precipitated with Synoviolin, strongly suggesting that nicastrin is the substrate of Synoviolin. Interestingly, amyloid b-protein generation was increased by the overexpression...
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Báo cáo khoa học: A new pathway encompassing calpain 3 and its newly identified substrate cardiac ankyrin repeat protein is involved in the regulation of the nuclear factor-jB pathway in skeletal muscle pdf
... binding totitin by improving the accessibility of the titin-bindingdomain. Interestingly, the importance of CARP inter-action for its function was recently demonstrated in aFig. 3. Calpain ... [3,14,15]. Two of theseregions, the N2A region and the M line, are involved in the transmission of mechanical signals to signaling path-ways. In the M line, mechanical stimulation activates the interaction ... NF-jBpathway and an increase in muscle fiber apoptosis [8]. The participation of NF-jB signaling in the pathogen-esis of LGMD2A is therefore an interesting possibility. The findings obtained in the present...
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Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt
... residues were individually mutated. (C) The spa-cial proximity of active site residues in the L-domain and connectingregion II which are potentially involved in the binding of D-glucose in the super-open ... binding; (b) to identify the activesite residues involved in the binding of Glc to the super-open state (Fig. 1C) of this two domain [large(L) and small (S)] enzyme, and thus the site of initia-tion ... pre-diction of hinge regions in the closed conformation(PDB identity: 1v4s), the highest score was obtainedfor residues in two of the regions connecting the L- and the S-domains [i.e. in connecting...
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Báo cáo khoa học: Towards understanding the functional role of the glycosyltransferases involved in the biosynthesis of Moraxella catarrhalis lipooligosaccharide ppt
... conse-quently the b-d-Gal was found in the terminal positionbecause the a-d-Gal found in the wild-type 2951 oligo-saccharide was missing. What was most surprising,however, was that mutation of the lgt4 ... shifts of the corresponding remainingring protons. Again, assignment of the Kdo residue was possible from examination of TOCSY correla-tions in the 120 ms1H-13C-HSQC-TOCSY spectrumfrom the ... conspicuous in the 1H-13C-HSQC spectrum.Examination of the anomeric13C line of residue C in the 120 ms1H-13C-HSQC-TOCSY spectrumrevealed the location of the remaining ring protons.CD...
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Báo cáo khoa học: Geranylgeranyl reductase involved in the biosynthesis of archaeal membrane lipids in the hyperthermophilic archaeon Archaeoglobus fulgidus potx
... Engineering, Graduate School of Engineering, Tohoku University, Miyagi, Japan The structure of membrane lipids is the most strikingcharacteristic of the Archaea (one of the three domains of ... DGGGPS). The mixture was incubatedat 55 °C for 1 h and then used directly in the GGR assay as the substrate mixture that mainly contains DGGGP. Tosynthesize the substrate mixtures containing mainly ... the protein arose from the translation from Met at the 24th position of the expec-ted full-length of the archaeal enzyme (except for the polyhistidine-tag). In contrast, a distinct protein of slightly...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25