what is involved in carrying out routine maintenance on equipment

Báo cáo khoa học: A novel prokaryotic L-arginine:glycine amidinotransferase is involved in cylindrospermopsin biosynthesis potx

... ornithineas amidino group acceptors. This is the case in l-arginine:glycine amidinotransferase which only haveone substrate-binding site, giving rise to the classicalping-pong mechanism and acceptance ... hydroxylamine-binding site. Thisbinding affects the rate of reaction by factor b, causingthe noncompetitive component of the mixed inhibition. In addition, binding of ornithine to this distinct sitealso alters ... product inhibition study was conducted to furtherdiagnose and conﬁrm the kinetic mechanism ofCyrA. Vertebrate l-arginine:glycine amidinotransferasedisplay strong product inhibition by ornithine,...

Báo cáo khoa học: Cotton GhMPK2 is involved in multiple signaling pathways and mediates defense responses to pathogen infection and oxidative stress doc

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... protein kinase) in tomato, are all involved in defense-related signal transduction [11]. In contrast,transposon inactivation of the Arabidopsis group BMPK4 gene conferred enhanced disease resistance ... Thus, this studysuggests a role for GhMPK2 in the defense signalingpathways in response to both pathogen infection andoxidative stress.Cotton GhMPK2 is involved in multiple signaling pathways ... roles in ROS homeostasis and that ROS-mediated injurymay be effectively alleviated by the induction ofGhMPK2. On the one hand, GhMPK2 was stronglyinduced in response to MV, which can cause continu-ous...

Báo cáo khoa học: An inserted loop region of stromal ascorbate peroxidase is involved in its hydrogen peroxide-mediated inactivation pot

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... spinach [5]. These results indicated that an inser-ted loop region of stromal APX is involved in itshydrogen peroxide-mediated inactivation.Discussion In this study, by using various chimeric APXsbetween ... have proved the hypothesisthat a region in the C-terminal half of stromal APX is involved in its susceptibility to hydrogen peroxideand indicated that this region is in a loop unique tochloroplastic ... [8]. This ﬁnding raised thehypothesis that a region within the C-terminal half ofthe chloroplastic APXs is involved in their susceptibil-ity to hydrogen peroxide.ResultsPreparation of chimeric...

Báo cáo khoa học: Oxidative stress is involved in the permeabilization of the inner membrane of brain mitochondria exposed to hypoxia/reoxygenation and low micromolar Ca2+ Lorenz Schild1 and Georg Reiser2 doc

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... reperfusion. A second factordetermining mitochondrial damage upon ischemia ⁄reperfusion is the increase in cytosolic Ca2+concentra-tion, which causes swelling of mitochondria, inductionof ... of Ca2+increase in combination with H ⁄ Randextramitochondrial NADH on respiration of brain mitochondria. Ratbrain mitochondria (0.5 mgặmL)1) were incubated at 30 °C in incu-bation medium. ... respiration(40.0 ± 7.4 vs. 84.5 nmol O2ặmin)1ặmg)1after NADHaddition).Discussion In animal models of stroke, mitochondria are injuredupon ischemia ⁄ reperfusion. This injury is characterizedby...

What is New in Nessus® v5.0? pptx

Danh mục: An ninh - Bảo mật

... Scan Execution Powerful new Nessus 5.0 features allow customers to take advantage of real-time scan results, with on- the-fly filtering and sorting, and streamlined results navigation. ... such as, Vulnerability Publication Date, public vulnerability database ID (OSVDB, Bugtraq, CERT Advisory, and Secunia), Plugin type (local or remote), information assurance vulnerability alert ... %Showing%Critical%and%High%Risks% Nessus%5.0%Host%Summary%w ith% 5%Levels%o f%Ri sk%Severity% Copyright â 2002-2012 Tenable Network Security, Inc. 6 (Nessus%5.0%Plugin%Filterin...

Báo cáo khoa học: Glycogen synthase kinase 3b and b-catenin pathway is involved in toll-like receptor 4-mediated NADPH oxidase 1 expression in macrophages ppt

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... GSK3bregulates TLR-mediated cytokine production and inac-tivation of GSK3b by LPS has a negative effect on production of the pro -in ammatory cytokine inter-feron-b [26]. b-Catenin is one of the most importantdownstream ... b-catenin signal-ing. In other words, LPS inactivates GSK3b throughphosphorylation, and the inactivated GSK3b inhibitsthe degradation of b-catenin, promoting translocationinto the nucleus. It is ... changes in GSK3b phosphorylation, using LPS stimulation, in J S. Kim et al. GSK3b-b-catenin is involved in Nox1 expressionFEBS Journal 277 (2010) 28302837 ê 2010 The Authors Journal compilation ê...

Báo cáo khoa học: An E3 ubiquitin ligase, Synoviolin, is involved in the degradation of immature nicastrin, and regulates the production of amyloid b-protein doc

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... Synoviolin increases the mem-brane localization of huntingtin protein [18], also sug-gesting that Synoviolin is involved in intracellulartrafﬁcking.We also found that NCT interacts with Synoviolin(Fig. ... Technical support issues arisingfrom supporting information (other than missing ﬁles)should be addressed to the authors.Synoviolin is involved in the degradation of nicastrin T. Maeda et al.5840 ... Synoviolin. Interestingly, amyloid b-protein generation was increased bythe overexpression of Synoviolin, although the nicastrin level was decreased.Thus, Synoviolin-mediated ubiquitination is involved...

Báo cáo khoa học: Sin3 is involved in cell size control at Start in Saccharomyces cerevisiae Octavian Stephan and Christian Koch ppt

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... activation (Fig. 6B). The timing of Sin3binding is therefore consistent with a role for Sin3 in repression of CLN transcription in the G1phase.ABCDEFGFig. 6. Dissociation of Sin3 from ... Cdc28 kinase were involved in the inactivation or repression ofSin3 ⁄ Rpd3-dependent histone deacetylation at theCLN1,2 promoters. These data raise several questionsconcerning the regulation of ... (Fig. 7).Discussion In this study, we provide evidence that SIN3 is involved in the correct timing of G1cyclin expression in Saccharomyces cerevisiae at the G1–S phase transi-tion. We have...

Báo cáo khoa học: Modulation of the endocannabinoid system by focal brain ischemia in the rat is involved in neuroprotection afforded by 17b-estradiol pdf

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... mechanism. In conclusion, this is theﬁrst report documenting the modulation of the endo-cannabinoid system by estrogen in the brain underpathologic conditions, leading to the suggestion that ... neonatal rats exposedto an intrastriatal microinjection of NMDA [41]. This is consistent with our data, documenting that systemicadministration of SR141716 results in a signiﬁcantreduction ... activation on ischemic dam-age. We found that administration of the CB1 recep-tor antagonist SR141716 (3 mgặkg)1, intraperitoneal),15 min before MCAo, resulted in a signiﬁcant reduc-tion in...

Báo cáo khoa học: Protein kinase Ch activity is involved in the 2,3,7,8tetrachlorodibenzo-p-dioxin-induced signal transduction pathway leading to apoptosis in L-MAT, a human lymphoblastic T-cell line potx

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... modiﬁcations, to examine the translocationof PKCh and PKCd during the TCDD-induced apoptosismechanism. In brief, L-MAT cells growing exponentially in RPMI 1640 containing 5% (v ⁄ v) fetal bovine ... lymphoblastic T-cell line. In this report, we providethe following evidence that the protein kinase C (PKC)h activity is func-tionally involved in the AhR-independent signal transduction mechanismthat ... or PKCh) is involved in the TCDD-induced apoptosis of L-MATcells.The incubation of L-MAT cells with 20 nm TCDDresulted in morphological changes characteristic ofapoptosis upon staining with...

Báo cáo khoa học: Lipid droplet and milk lipid globule membrane associated placental protein 17b (PP17b) is involved in apoptotic and differentiation processes of human epithelial cervical carcinoma cells pptx

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... that a gene involved in squamous celldifferentiation can be effectively induced by PMA usingAP-1 binding sites, and its expression is inhibited by PKCinhibitors [61]. This is also consistent with ... wassigniﬁcantly inhibited by protein kinase C (PKC) inhibitor,indicating the involvement of NF-jB and AP-1 transcriptionfactors in this process, while protein kinase A (PKA)inhibitor had only a modest inhibitory ... measured in apoptotic conditions, treating cells with carboplatin,5-ﬂuorouracil, irinotecan, mitomycin or paclitaxel in clini-cally achievable concentrations, in various dose–timecombinations....

Báo cáo Y học: The N-terminus of m5C-DNA methyltransferase Msp I is involved in its topoisomerase activity docx

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... result in modiﬁcation/truncation of the N-terminalportion only. Indeed, on HNBB modiﬁcation (50-foldexcess HNBB at pH 8.5), the N-terminal portion of theprotein is truncated, which was conﬁrmed ... d.p.m.Protein analysisProtein concentration was determined on the basis ofBradford’s principle [24] using the Bio-Rad Coomassie pluskit. Standard curves were established using BSA. Proteinsamples ... N-terminal sequence. In mouse m5C-MTase, the N-terminal domain is > 1000 amino acids[11,12]. Within the N-terminal domain, a DNA replicationfoci-targeting domain has been identiﬁed in mouse...

Báo cáo khoa học: A new pathway encompassing calpain 3 and its newly identiﬁed substrate cardiac ankyrin repeat protein is involved in the regulation of the nuclear factor-jB pathway in skeletal muscle pdf

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... theseregions, the N2A region and the M line, are involved in the transmission of mechanical signals to signaling path-ways. In the M line, mechanical stimulation activatesthe interaction of a protein ... stronglysuggest that, once cleaved, CARP interaction with theregion N2A is reinforced. CARP interacts with titin-N2A using a region situated in its second ankyrinrepeat (Fig. 1B) [18]. Bio-informatics ... calpain 3 wasinvestigated. A direct, in vitro digestion of CARP bycalpain 3 using recombinant proteins could not beattempted because calpain 3 is inactivated during puriﬁ-cation [45]. In skeletal...

Báo cáo khoa học: Myocyte enhancer factor 2B is involved in the inducible expression of NOX1⁄ NADPH oxidase, a vascular superoxide-producing enzyme ppt

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... muscle cell line. A PGF2a-response element waslocated between )146 and )125 in the 5Â-anking region containing aconsensus binding site for myocyte enhancer factor 2 (MEF2), to whichbinding of ... documented in VSMC has been somewhat con-troversial. MEF2A was reported to be involved in anangiotensin II-induced vascular hypertrophy [15], and in inducing the expression of monocyte chemo-attractant ... modulationof VSMC; (b) the promoter region of the NOX1 genecontained a consensus MEF2-binding site that confersthe responsiveness to PGF2a; (c) stimulation withPGF2aenhanced the binding of...