what is involved in carrying out routine maintenance on equipment

... ornithine as amidino group acceptors. This is the case in l-arginine:glycine amidinotransferase which only have one substrate-binding site, giving rise to the classical ping-pong mechanism and acceptance ... hydroxylamine-binding site. This binding affects the rate of reaction by factor b, causing the noncompetitive component of the mixed inhibition. In addition, binding of ornithine to this distinct site also alters ... product inhibition study was conducted to further diagnose and confirm the kinetic mechanism of CyrA. Vertebrate l-arginine:glycine amidinotransferase display strong product inhibition by ornithine,...

Ngày tải lên: 06/03/2014, 22:21

... protein kinase) in tomato, are all involved in defense-related signal transduction [11]. In contrast, transposon inactivation of the Arabidopsis group B MPK4 gene conferred enhanced disease resistance ... Thus, this study suggests a role for GhMPK2 in the defense signaling pathways in response to both pathogen infection and oxidative stress. Cotton GhMPK2 is involved in multiple signaling pathways ... roles in ROS homeostasis and that ROS-mediated injury may be effectively alleviated by the induction of GhMPK2. On the one hand, GhMPK2 was strongly induced in response to MV, which can cause continu- ous...

Ngày tải lên: 14/03/2014, 23:20

... spinach [5]. These results indicated that an inser- ted loop region of stromal APX is involved in its hydrogen peroxide-mediated inactivation. Discussion In this study, by using various chimeric APXs between ... have proved the hypothesis that a region in the C-terminal half of stromal APX is involved in its susceptibility to hydrogen peroxide and indicated that this region is in a loop unique to chloroplastic ... [8]. This finding raised the hypothesis that a region within the C-terminal half of the chloroplastic APXs is involved in their susceptibil- ity to hydrogen peroxide. Results Preparation of chimeric...

Ngày tải lên: 16/03/2014, 14:20

... reperfusion. A second factor determining mitochondrial damage upon ischemia ⁄ reperfusion is the increase in cytosolic Ca 2+ concentra- tion, which causes swelling of mitochondria, induction of ... of Ca 2+ increase in combination with H ⁄ Rand extramitochondrial NADH on respiration of brain mitochondria. Rat brain mitochondria (0.5 mgặmL )1 ) were incubated at 30 °C in incu- bation medium. ... respiration (40.0 ± 7.4 vs. 84.5 nmol O 2 ặmin )1 ặmg )1 after NADH addition). Discussion In animal models of stroke, mitochondria are injured upon ischemia ⁄ reperfusion. This injury is characterized by...

Ngày tải lên: 16/03/2014, 22:20

... Scan Execution Powerful new Nessus 5.0 features allow customers to take advantage of real-time scan results, with on- the-fly filtering and sorting, and streamlined results navigation. ... such as, Vulnerability Publication Date, public vulnerability database ID (OSVDB, Bugtraq, CERT Advisory, and Secunia), Plugin type (local or remote), information assurance vulnerability alert ... %Showing%Critical%and%High%Risks% Nessus%5.0%Host%Summary%w ith% 5%Levels%o f%Ri sk%Severity% Copyright â 2002-2012 Tenable Network Security, Inc. 6 ( Nessus%5.0%Plugin%Filterin...

Ngày tải lên: 22/03/2014, 15:21

... GSK3b regulates TLR-mediated cytokine production and inac- tivation of GSK3b by LPS has a negative effect on production of the pro -in ammatory cytokine inter- feron-b [26]. b-Catenin is one of the most important downstream ... b-catenin signal- ing. In other words, LPS inactivates GSK3b through phosphorylation, and the inactivated GSK3b inhibits the degradation of b-catenin, promoting translocation into the nucleus. It is ... changes in GSK3b phosphorylation, using LPS stimulation, in J S. Kim et al. GSK3b-b-catenin is involved in Nox1 expression FEBS Journal 277 (2010) 28302837 ê 2010 The Authors Journal compilation ê...

Ngày tải lên: 22/03/2014, 21:21

... Synoviolin increases the mem- brane localization of huntingtin protein [18], also sug- gesting that Synoviolin is involved in intracellular trafficking. We also found that NCT interacts with Synoviolin (Fig. ... Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Synoviolin is involved in the degradation of nicastrin T. Maeda et al. 5840 ... Synoviolin. Interestingly, amyloid b-protein generation was increased by the overexpression of Synoviolin, although the nicastrin level was decreased. Thus, Synoviolin-mediated ubiquitination is involved...

Ngày tải lên: 23/03/2014, 04:20

... activation (Fig. 6B). The timing of Sin3 binding is therefore consistent with a role for Sin3 in repression of CLN transcription in the G 1 phase. A B C D E F G Fig. 6. Dissociation of Sin3 from ... Cdc28 kinase were involved in the inactivation or repression of Sin3 ⁄ Rpd3-dependent histone deacetylation at the CLN1,2 promoters. These data raise several questions concerning the regulation of ... (Fig. 7). Discussion In this study, we provide evidence that SIN3 is involved in the correct timing of G 1 cyclin expression in Saccharomyces cerevisiae at the G 1 –S phase transi- tion. We have...

Ngày tải lên: 23/03/2014, 05:22

... mechanism. In conclusion, this is the first report documenting the modulation of the endo- cannabinoid system by estrogen in the brain under pathologic conditions, leading to the suggestion that ... neonatal rats exposed to an intrastriatal microinjection of NMDA [41]. This is consistent with our data, documenting that systemic administration of SR141716 results in a significant reduction ... activation on ischemic dam- age. We found that administration of the CB1 recep- tor antagonist SR141716 (3 mgặkg )1 , intraperitoneal), 15 min before MCAo, resulted in a significant reduc- tion in...

Ngày tải lên: 23/03/2014, 07:20

... modifications, to examine the translocation of PKCh and PKCd during the TCDD-induced apoptosis mechanism. In brief, L-MAT cells growing exponentially in RPMI 1640 containing 5% (v ⁄ v) fetal bovine ... lymphoblastic T-cell line. In this report, we provide the following evidence that the protein kinase C (PKC)h activity is func- tionally involved in the AhR-independent signal transduction mechanism that ... or PKCh) is involved in the TCDD-induced apoptosis of L-MAT cells. The incubation of L-MAT cells with 20 nm TCDD resulted in morphological changes characteristic of apoptosis upon staining with...

Ngày tải lên: 23/03/2014, 13:20

... that a gene involved in squamous cell differentiation can be effectively induced by PMA using AP-1 binding sites, and its expression is inhibited by PKC inhibitors [61]. This is also consistent with ... was significantly inhibited by protein kinase C (PKC) inhibitor, indicating the involvement of NF-jB and AP-1 transcription factors in this process, while protein kinase A (PKA) inhibitor had only a modest inhibitory ... measured in apoptotic conditions, treating cells with carboplatin, 5-fluorouracil, irinotecan, mitomycin or paclitaxel in clini- cally achievable concentrations, in various dose–time combinations....

Ngày tải lên: 23/03/2014, 21:20

... result in modification/truncation of the N-terminal portion only. Indeed, on HNBB modification (50-fold excess HNBB at pH 8.5), the N-terminal portion of the protein is truncated, which was confirmed ... d.p.m. Protein analysis Protein concentration was determined on the basis of Bradford’s principle [24] using the Bio-Rad Coomassie plus kit. Standard curves were established using BSA. Protein samples ... N-terminal sequence. In mouse m5C- MTase, the N-terminal domain is > 1000 amino acids [11,12]. Within the N-terminal domain, a DNA replication foci-targeting domain has been identified in mouse...

Ngày tải lên: 24/03/2014, 00:21

... these regions, the N2A region and the M line, are involved in the transmission of mechanical signals to signaling path- ways. In the M line, mechanical stimulation activates the interaction of a protein ... strongly suggest that, once cleaved, CARP interaction with the region N2A is reinforced. CARP interacts with titin- N2A using a region situated in its second ankyrin repeat (Fig. 1B) [18]. Bio-informatics ... calpain 3 was investigated. A direct, in vitro digestion of CARP by calpain 3 using recombinant proteins could not be attempted because calpain 3 is inactivated during purifi- cation [45]. In skeletal...

Ngày tải lên: 29/03/2014, 21:20

... muscle cell line. A PGF 2a -response element was located between )146 and )125 in the 5Â-anking region containing a consensus binding site for myocyte enhancer factor 2 (MEF2), to which binding of ... documented in VSMC has been somewhat con- troversial. MEF2A was reported to be involved in an angiotensin II-induced vascular hypertrophy [15], and in inducing the expression of monocyte chemo- attractant ... modulation of VSMC; (b) the promoter region of the NOX1 gene contained a consensus MEF2-binding site that confers the responsiveness to PGF 2a ; (c) stimulation with PGF 2a enhanced the binding of...

Ngày tải lên: 30/03/2014, 03:20

Ngày tải lên: 31/03/2014, 01:20

Ngày tải lên: 31/03/2014, 09:20

Ngày tải lên: 19/06/2014, 22:20

Ngày tải lên: 19/06/2014, 22:20

Ngày tải lên: 20/06/2014, 01:20