visualization of mobility by atomic force microscopy

... dynamics of supercoil-stabilized DNA cruciforms J Mol Biol 280, 61–72 Lyubchenko, Y.L., Jacobs, B.L., Lindsay, S.M & Stasiak, A (1995) Atomic- force microscopy of nucleoprotein complexes Scanning Microscopy ... effect of deformation on the lateral resolution of atomic force microscopy J Microsc 182, 106–113 Cherny, D.I., Fourcade, A., Svinarchuk, F., Nielsen, P.E., Malvy, C & Delain, E (1998) Analysis of ... chromosome folding by modulating the local structure of DNA regions and the global topology of chromatin domains ACKNOWLEDGEMENTS This work was supported in part by the fund from the Ministry of Education,...

Ngày tải lên: 31/03/2014, 23:20

... were of a functional feature of DC maturation The amount of IL-12 in culture supernatants of BMDCs was assessed by ELISA Compared with the control, LPS Page of promoted significantly secretion of ... the adhesive force of the immature BMDCs treated with 10.0 μg/L of GM-CSF plus 10.0 μg/L of IL-4 (B a) or the mature BMDCs stimulated with 1.0 mg/L of LPS (B b) The measured adhesion force (352.37 ... using atomic force microscopy Biochem Biophys Res Commun 2008, 374:90-94 Wu Y, Hu Y, Cai J, Ma S, Wang X, Chen Y, Pan Y: Time-dependent surface adhesive force and morphology of RBC measured by AFM...

Ngày tải lên: 21/06/2014, 02:20

... nanotubes by wet chemical procedure J Phys D Appl Phys 2010, 43:474013 24 Houzé F, Meyer R, Schneegans O, Boyer L: Imaging the local electrical properties of metal surfaces by atomic force microscopy ... CNTs and substrate Abbreviations AFM: atomic force microscope; AuNPs: gold nanoparticles; CNTs: carbon nanotubes; CP-AFM: conductive probe atomic force microscopy; CVD: chemical vapour deposition; ... precisely the resistance of individual CNTs Conclusions Conducting probe atomic force microscopy in ambient air was used to investigate the local electrical resistance of MWCNTs disposed on thin...

Ngày tải lên: 21/06/2014, 04:20

... doi:10.1186/1477-3155-8-10 Cite this article as: Breitenstein et al.: Immobilization of different biomolecules by atomic force microscopy Journal of Nanobiotechnology 2010 8:10 ... contact force The tip remained in contact for seconds and changed to the next spotting positions by retraction Ink was supplied by a hypodermic needle of Popper & Sons, Inc (N.Y 11040 USA) Microscopy ... substance, regardless of what kind of biotinylated mixture of biomolecules is to be arranged Two completely different species of molecules have been used to demonstrate the sequence of working steps:...

Ngày tải lên: 11/08/2014, 00:22

... composition Different types of LH antenna complexes and RCs from Rhodob sphaeroides have been structurally analyzed by X-ray crystallography, cryoelectron microscopy, and atomic force microscopy (AFM) [3,13–16] ... orientation The tilt of LH2 dimers was further studied by measuring the protruding profiles of each LH2 within the dimer on the basis of the height differences of both sides and the size of LH2’s (Fig ... core complex of Rhodobacter blasticus by in situ atomic force microscopy J Biol Chem 280, 1426–1431 Scheuring S, Goncalves RP, Prima V & Sturgis JN ¸ (2006) The photosynthetic apparatus of Rhodopseudomonas...

Ngày tải lên: 18/02/2014, 18:20

... characterization of supported lipid lms with the atomic force microscope Biochim Biophys Acta 1509, 1441 16 Rinia HA & de Kruijff B (2001) Imaging domains in model membranes with atomic force microscopy ... weights of individual proteins correlate with molecular volumes measured by atomic force microscopy Puăgers Arch 435, 362367 25 Geisse NA, Cover TL, Henderson RM & Edwardson JM (2004) Targeting of ... the atomic force microscope Nat Struct Biol 7, 715718 29 Schneider S, Folprecht G, Krohne G & Oberleithner H (1995) Immunolocalization of lamins and nuclear pore complex proteins by atomic force...

Ngày tải lên: 16/03/2014, 02:20

... presence of molecules For instance, morphology of organic molecules can be easily influenced by the tip shape that has comparable dimensions to the molecules [18] and by the forces exerted by the ... film Appl Phys Lett 2004, 85:3626-3628 Page 10 of 10 12 Moreno-Herrero F, Perez M, Baro AM, Avila J: Characterization by atomic force microscopy of alzheimer paired helical filaments under physiological ... West P: Atomic Force Microscopy New York: Oxford University Press; 2010, ISBN: 978-0199570454 14 Kühle A, Sørensen AH, Zandbergen JB, Bohr J: Contrast artifacts in tapping tip atomic force microscopy...

Ngày tải lên: 21/06/2014, 04:20

... of the in-situ electron beam-induced selforganization of gold nanoclusters in SiO2 J Phys D 2009, 42:075304 17 Ruffino F, Grimaldi MG: Atomic force microscopy study of the growth mechanisms of ... developed by Sigsbee [42] Ostwald ripening is regulated by the vapor pressure at the surfaces of the cluster, P(R), depending on the curvature of the surface and it is driven by the minimization of ... radius l of a depletion zone at time t is simply the atomic diffusion length: l D st (3) The time dependence of the cluster growth expressed by Equation is determined by the dimensionality of the...

Ngày tải lên: 21/06/2014, 05:20

... a sample of vertical SiNWs on n-type Si wafer with diameters in the range of 50-100 nm The length of wires after planarization was estimated around μm Conductive-probe atomic force microscopy ... electrical properties of metal surfaces by atomic force microscopy with conducting probes Appl Phys Lett 1996, 69:1975 13 Yu L, Rocai Cabarrocas P: Initial nucleation and growth of in-plane solidliquid-solid ... tenths of SiNWs An example of cross-sectional profiles involving SiNWs is illustrated in Figure The conducting wires are easily put in evidence with a decrease of the local resistance by several...

Ngày tải lên: 21/06/2014, 06:20

... described, and Page of 13 the final concentration of Ad was determined using ε 414 = 9.8 mM-1 cm-1 [23] The purity of the Ad preparation was estimated by determining the relative absorbance of the protein ... μl of obtained solution were immediately deposited onto the freshly cleaved mica surface and left for For visualization of the individual Ad and AdR protein molecules, μl of 1.0 μM solution of ... by use of N5 Submicron Particle Size Analyzer (Beckman Coulter, Inc) The principle of registration is based on measuring the interference pattern of light scattered on particles in solution by...

Ngày tải lên: 21/06/2014, 11:20

... by the AFM images reported in Fig 1: a for the sample with a thickness of nm b of nm, c of 14 nm, d of 20 nm, e of 26 nm, f of 32 nm of Au respectively The continuous lines represent the fit by ... dimension of the cross section through the data, which in this case equals Figure reports for each power spectra the fit by Eq of the linear region (continuous lines) The values of ci were obtained by ... roughness r for each sample by the corresponding AFM images using the XEI software In particular, the value of r for each sample was calculated by averaging the values obtained by five lm AFM images...

Ngày tải lên: 22/06/2014, 01:20

... pathology of human sperm by atomic force microscopy Fertil Steril 2001, 75:961-965 Joshi NV, Medina H, Osuna JA: Ultrastructural pathology of varicocele spermatozoa by using atomic force microscopy ... mapping of living cells by atomic force microscopy Biophys J 1998, 74:1564-1578 Rudd RE, McElfresh M, Baesu E, Balhorn R, Alleny M, Belak J: Modeling of the Deformation of Living Cells Induced by Atomic ... readout of flash x-ray images of living sperm in water by atomic- force microscopy Science 1991, 252:691-693 Allen MJ, Lee C, Lee JD IV, Pogany GC, Balooch M, Siekhaus WJ, Balhorn R: Atomic force microscopy...

Ngày tải lên: 11/08/2014, 00:22

... Molecular Force Spectroscopy Techniques Chapter Atomic Force Microscopy and Single-Molecule Force Spectroscopic Techniques Ever since the invention of the atomic force microscope (AFM) in 1986 by Binning ... Mechanism of Cytoadherence of P falciparum IRBCs 13 1.3 Objective and Scope of This Thesis 24 1.3.1 Objectives 24 1.3.2 Scope of Project 25 CHAPTER ATOMIC FORCE MICROSCOPY ... ATOMIC FORCE MICROSCOPY STUDY OF MALARIA INFECTED RED BLOOD CELLS LI ANG (B S., FUDAN UNIVERSITY, CHINA) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MACHANICAL...

Ngày tải lên: 11/09/2015, 14:34

...   1.1.2 Atomic force microscopy In the 1980s, a series of microscopy with similar principles were invented, and they  formed the family of scanning probe microscopy (SPM). As a member of this family,  ... physical contact with the cell surface and apply a force on it. Cells are soft and are  easily deformed by these forces applied. The effect of these forces on image quality  was examined by scanning the same area at increasing forces. The cell was further  ... TABLE OF CONTENTS ii SUMMARY v LIST OF FIGURES vii LIST OF TABLES ix CHAPTER INTRODUCTION 1.1 Background 1.1.1 Cancer and metastasis 1.1.2 Atomic force microscopy 1.1.3 Anti-cancer properties of...

Ngày tải lên: 30/09/2015, 14:23

... Akiba, U., and Fujihira, M (2000) Chemical force microscopy of microcontact-printed self-assembled monolayers by pulsedforce-mode atomic force microscopy Ultramicroscopy 82, 203–212 Imaging Methods ... (1995) Improvement of thermally induced bending of cantilevers used for atomic force microscopy Scanning 17, 117–121 Imaging Methods in AFM 13 Imaging Methods in Atomic Force Microscopy Davide ... hydration forces in electrolyte solutions with an atomic force microscope Biophys J 60, 1438–1444 23 Vinckier, A and Semenza, G (1998) Measuring elasticity of biological materials by atomic force microscopy...

Ngày tải lên: 11/04/2014, 02:07

... schematic of the scratch direction (b) The AFM images of the typical grooves scratched at 10 μN of tip force, μm/s of speed (c) The cross-section profiles of the grooves at the position as indicated by ... number of scratch or scan cycle (a) The AFM images of the scratches at 10 μN of applied force and μm/s of scratch speed in the forward scratch (b) Correlation of d and Wf data with numbers of scratching ... Nanolithography of single-layer graphene oxide films by atomic force microscopy Langmuir 2010, 26:6164-6166 Versen M, Klehn B, Kunze U, Reuter D, Wieck AD: Nanoscale devices fabricated by direct machining of...

Ngày tải lên: 21/06/2014, 00:20

... the curvature radius of *35 nm, the force constant of 34 N m-1, and the resonance frequency of 350 kHz, respectively The sample was a p-GaAs(100) wafer with the resistivity of 10 X cm and root-mean-square ... quantitative account of the growth kinetics, in Fig 2b, the growth rate of oxide nanodots is plotted as a function of electric field strength The initial growth rate of oxide nanodots induced by the CNT-attached ... comparison The results show that, in both cases, the height of the oxides increases by a factor of *1.1 Similar tendency of enhancement in the height of oxide structures under AC conductions was observed...

Ngày tải lên: 22/06/2014, 01:20

... previously unaware of, are determined by the properties of the single elements of such ensembles II Scanning Tunneling Microscopy It is of particular interest to understand the images of biological ... Tunneling Microscopy Atomic Force Microscopy Force Spectroscopy Photonic Force Microscopy References 13 21 30 The Atomic Force Microscope in the Study of Membrane Fusion and Exocytosis Bhanu P Jena ... Cells Identification of New Plasma Membrane Structures Involved in Exocytosis Future of AFM in the Study of Live Cells References 33 35 37 39 47 48 Atomic Force Microscope Imaging of Cells and Membranes...

Ngày tải lên: 27/06/2014, 08:20

... Akiba, U., and Fujihira, M (2000) Chemical force microscopy of microcontact-printed self-assembled monolayers by pulsedforce-mode atomic force microscopy Ultramicroscopy 82, 203–212 Imaging Methods ... (1995) Improvement of thermally induced bending of cantilevers used for atomic force microscopy Scanning 17, 117–121 Imaging Methods in AFM 13 Imaging Methods in Atomic Force Microscopy Davide ... hydration forces in electrolyte solutions with an atomic force microscope Biophys J 60, 1438–1444 23 Vinckier, A and Semenza, G (1998) Measuring elasticity of biological materials by atomic force microscopy...

Ngày tải lên: 27/06/2014, 08:20

... previously unaware of, are determined by the properties of the single elements of such ensembles II Scanning Tunneling Microscopy It is of particular interest to understand the images of biological ... Tunneling Microscopy Atomic Force Microscopy Force Spectroscopy Photonic Force Microscopy References 13 21 30 The Atomic Force Microscope in the Study of Membrane Fusion and Exocytosis Bhanu P Jena ... Cells Identification of New Plasma Membrane Structures Involved in Exocytosis Future of AFM in the Study of Live Cells References 33 35 37 39 47 48 Atomic Force Microscope Imaging of Cells and Membranes...

Ngày tải lên: 27/06/2014, 17:20