vi— expression of mucin genes in gallstone disease

... change in expression of IL-10 at dpi was 28.8-fold higher (P = 0.03) In contrast, the expression of IL-5 mRNA in the bursa of birds infected with the H strain increased continuously, peaking at ... their role in the pathogenesis of such infections has not yet been extensively studied Of the seven genes examined in this study, the levels of expression of IFN-g, IL-2 and IL12P40 genes in the ... expression during IBDV infection Infection with IBDV resulted in transcriptional changes of mRNA encoding IFN-g, IL-2 and IL-12p40 during the acute phase of the disease Differences in cytokine expression...

Ngày tải lên: 11/08/2014, 21:21

... role of the N-terminal part of the kinase, containing a PAS domain, was established in signal transduction between the RH and the kinase [6,7] Addition of H2 to HupUV before or during the incubation ... single point mutation in the helix–turn–helix DNA binding motif of PpsR, rendering it inactive [26] Comparison of HupSL regulations and the functional roles of HupTUV in other T roseopersicina ... transcript level of the hupTUV genes is below the detection limit or is missing in T roseopersicina Mutagenesis and homologous expression of the hupT and hupTUV genes In- frame deletion mutagenesis was...

Ngày tải lên: 16/03/2014, 23:20

... points following infection with varying MOIs Analysis of recombinant protein production in these samples will indicate how much virus is needed to obtain maximal yield after a given time of infection ... with the intended use (in vitro, in vivo, structural biology) The design of a suitable expression vector for an expression system of choice, also including a potential "tagging" of the protein to ... as neomycin or hygromycin, positive transfectants can be selected in media containing the corresponding antibiotic Induction of gene amplification using glutamine synthetase22 or dihydrofolate...

Ngày tải lên: 11/04/2014, 09:41

... The use of fenofibrate was related to significant decreases in the expression in circulating leukocytes of CX3CL1 and CCR2 genes and a significant increase in the expression of SDF-1 Page of (page ... We did not find any significant effect of fenofibrate in the MCP-1/CCL2 gene expression However, there was a significant decrease in the gene expression of the CX3CL1/ Fractalkine in patients ... change in the expression of chemokine receptors CX3CR1 and CXCR4 but the expression of CCR2 was significantly decreased in patients treated with fenofibrate (U-Mann-Whitney, p = 0.016; Figure 1B) Of...

Ngày tải lên: 10/08/2014, 05:21

... regulator of viral gene expression and essential for virion production (Gruffat et al., 2002) SM protein can activate intronless genes expression and inhibit expression of intron-containing genes ... activating expression of some but not all intronless genes (Ruvolo et al., 2001) Except regulation of viral gene expression, SM protein can increase expression of some cell genes, such as some interferon-stimulated ... In contrast to the majority of cellular genes, many EBV genes expressed during lytic cycle are intronless, and SM may therefore be important in enhancing expression of other lytic EBV genes In...

Ngày tải lên: 05/10/2015, 22:32

... Cotto et al Expression of prion genes in the developing zebrafish Fig Alignment of the conserved sequence motifs of the C-terminal domain among members of the PrP family Amino acid numbering starts ... be of interest because in terms of function, the fish intestine is highly regionalized both in the larva and in the adult [38] The posterior segment of the fish intestine is the absorption site of ... routing of PrPSc in the early steps of infection Our observations of a prp2 hybridization signal in the posterior intestine of zebrafish larvae call for evaluation of the potential prions uptake of...

Ngày tải lên: 19/02/2014, 16:20

... dissemination of SARS-CoV By slowing down expression of IFNs and their antiviral genes in the infected tissue cells, the virus buys time during the initial, critical phase of infection in order ... production of chemokines by the infected tissue cells Results Virus-induced cytokines not only play a significant role in host defense, but also in immunopathogenesis Investigations of the cytokine profiles ... rapid rise in virus titers during the initial stage of disease, caused by the suppression of antiviral cytokines, as well as the progressive infiltration of immune cells into the infected lungs,...

Ngày tải lên: 20/06/2014, 01:20

... nucleotide binding site (NBS) domain adjacent to the Nterminus and a leucine-rich repeat (LRR) domain involved in the host recognition of pathogen-derived elicitors NBS-LRRs constitute one of the ... protein sequence motifs in predicted Lolium NBS domains aMotifs listed in the order of occurrence in the NBS domain of putative perennial ryegrass R genes Perennial ryegrass motifs were named in ... at a mean of 2.5 per primary template sequence, with a range from 1– 12 A total of 102 distinct putative R gene sequences (corresponding to 99 NBS-containing genes and receptor kinase genes) were...

Ngày tải lên: 12/08/2014, 03:20

... proteins indeed changes the expression of apoptosis profile of host cells, which probably results in the carcinogenesis of liver cells Materials and methods Cell lines and isolation of RNA HepG2 ... the expression of cIAP2 was obviously higher in the HepG2.215, a cell line expressing HBV, than in the HepG2, a cell line absence of HBV (Figure 1A, 1&2) The up-regulation of cIAP2 expression in ... important regulators of cell apoptosis, it is possible that HBV infection might alter the cIAPs gene expressing of liver cell, finally instigating the carcinogenesis of the cells it infected This is...

Ngày tải lên: 02/11/2012, 11:17

... bacteria in these conditions The time courses of NH4+-N and NO2+3 N in batch experiment are shown in Fig Gradual decrease in NH4+-N and increase in NO2+3 N were observed just after the beginning of ... gene of AOB belonging to the ß subclass of Proteobacteria The thermal profiles included an initial denaturing step at 95°C for 10 followed by 45 cycles of denaturation at 95°C for 20 s, annealing ... of amoA mRNA in Run should be related to the fact that 40% of ammonia remained at the end of batch experiment Small decrease in the amount of amoA mRNA in Run was observed due to low dosage of...

Ngày tải lên: 05/09/2013, 10:15

... and eosin staining We examined the expression of these proteins in a cancer-free layer of squamous epithelial cells, carcinoma in situ (CIS) and invasive cancer, all of which were observed in the ... uterine cervix Although it remains to be determined how enhanced expression of Mcm proteins affects DNA replication in cancer cells, the following findings suggest a possible involvement of Mcm in ... comparing the amounts of Mcm in total cellular proteins and chromatin-bound proteins, approximately one-third of total Mcm protein was found to be recovered in the chromatin-bound fraction in HeLa...

Ngày tải lên: 20/02/2014, 23:20

... under the control of the GAL10 promoter The induction of the yeast, by galactose containing media, results in overexpression of GAL4, in turn inducing an increase of the expression of the OR gene ... level of I7 receptor expression is obtained in induced strains at 15 °C while the levels of Golf and Ga15 not change significantly In addition, the protein levels of I7 OR, Golf and Ga15 in strains ... function of increasing amount of total proteins in membrane fractions from I7 yeast strain induced at 15 °C, and from yeast expressing SSTR2 as a control activity By taking advantage of structural...

Ngày tải lên: 07/03/2014, 16:20

... protein metabolism in these cells Histone is rich in the basic amino acids, lysine and arginine, but contains no cysteine Protamine, which is replaced for hitstone via transit proteins during ... protein and the mRNA were faint Effects of inhibitors of cGCS and GR on primary cultured testicular cells To investigate the contribution of de novo synthesis and the recycling of GSH to the intracellular ... Thus, a high expression of GR in Sertoli cells can be fully confirmed Changes of GR expression in testes during sexual maturation To examine relationships between the expression of GR with sexual...

Ngày tải lên: 17/03/2014, 17:20

... diagram of the fusion of cells producing individual protein units of a multisubunit protein Three transfected CHO-K1 cell lines producing either IgA, J-chain or SC, respectively, were fused in two ... thereby eliminate the positional effect [30] of chromosomal integrating vectors However, for stable expression of multiple genes over time, the usefulness of such episomal vector is limited, mainly ... were fixed and stained with an anti(human J-chain) Ig to verify the presence of intracellular J-chain A further attempt to directly quantify the amount of intracellular J-chain was avoided, as...

Ngày tải lên: 18/03/2014, 01:20

... factor) Interleukin 1, beta Mitogen-activated protein kinase 14 Mitogen-activated protein kinase 13 Mitogen-activated protein kinase Mitogen-activated protein kinase Mitogen-activated protein kinase ... Mitogen-activated protein kinase Rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant) Rho-associated, coiled-coil containing protein kinase Ras-related C3 botulinum toxin substrate ... enzymes that in uence transcription by selectively deacetylating or acetylating the e-amino groups of lysine located near the amino termini of core histone proteins Acetylation of p65 at lysines 218,...

Ngày tải lên: 22/03/2014, 16:20

... or a combination of both (Fig 1) In the following, such in vivo models are described which address certain aspects of membrane topology, folding, intracellular targeting and trafficking of PrP The ... mediate an interaction of PrPC with accessory proteins, such as transmembrane proteins involved in PrP-induced signaling Alternatively, deletion of the HD could indirectly affect intermolecular interactions ... basolaterally, indicating that this domain acts as a dominant sorting signal Vice versa, Dpl or PrPC lacking the HD were found mainly at the apical surface of MDCK cells [36] An interesting activity of PrPC...

Ngày tải lên: 30/03/2014, 09:20

... may be minimizing the risk of insertional mutagenesis In addition to the potential for intracellular mislocalisation of protein, the addition of 2A peptide [[17] additional amino acids in this ... to link two genes transcribed from a single promoter within recombinant viral vectors However, there are a number of limitations using IRES elements, including their size and variability in expression ... revealed inconsistent levels of expression of individual transgenes within the expression cassette From our data summarized in Table 2, it is clear that the relative levels of MGMT and eGFP expression...

Ngày tải lên: 19/06/2014, 08:20

... may be minimizing the risk of insertional mutagenesis In addition to the potential for intracellular mislocalisation of protein, the addition of 2A peptide [[17] additional amino acids in this ... to link two genes transcribed from a single promoter within recombinant viral vectors However, there are a number of limitations using IRES elements, including their size and variability in expression ... revealed inconsistent levels of expression of individual transgenes within the expression cassette From our data summarized in Table 2, it is clear that the relative levels of MGMT and eGFP expression...

Ngày tải lên: 20/06/2014, 04:20

... variety of different groups of sepsis mediators: inflammatory cytokine interleukin-6 (IL-6), anti-inflammatory cytokine interleukin-10 (IL-10), chemokine interleukin-8 (IL-8), intercellular adhesion ... threshold, to promote and maintain rolling interactions [28] In our study, expression of L-selectin was depressed in the HT group This finding is consistent with previous findings and suggests that ... Annals of Intensive Care 2011, 1:44 http://www.annalsofintensivecare.com/content/1/1/44 in trauma and sepsis; these effects however have not yet been convincingly shown in clinical studies [5,6] In...

Ngày tải lên: 20/06/2014, 23:20

... the sequence of CaMV35S promoter which located infront of AtAOS2 gene in pCAMBIA1201/AtAOS binary vector (Fig 3) PCR was carried out according to the PCR Amplification Kit by using HiPi PCR Premix ... level of endogenous JA in rice, we try to over express AOS genes by transforming AtAOS2 gene to the rice genome Fig The octadecanoid pathway leading to jasmonic acid (JA) biosynthesis in plants ... Dr.Eunsun Kim) In this study, a set of PCRs was carried out to detect the hygromycin resistance gene, CaMV35S promoter (the promoter located in front of the AtAOS2 gene), and the AtAOS2 gene in the transgenic...

Ngày tải lên: 06/08/2014, 18:21