vector systems for expression of natural products

... strategy for expression of LASV proteins GP1, GP2, and NP in E coli using pMAL vectors Cloning Cloning strategy for expression of LASV proteins GP1, GP2, and NP in E coli using pMAL vectors To ... recombinant protein per gram of cell mass in an environment appropriate for generation of conformationally correct protein LASV NP expression also benefited from the use of vector pMALc2x; however, ... 0.2 g/L of uridine, g/L of glucose, 0.372 mL/L of Dawes Trace 1, 2.14 mL/L of Dawes Trace 2, 0.072 mL/L of Dawes Trace 3, 0.606 mL/L of M calcium chloride dihydrate (CaCl2-2H2O), 0.30 mL/L of 0.43...

Ngày tải lên: 20/06/2014, 01:20

... PCR products up to 350 bp in length when used at a concentration of 4.5% Such gels can be useful for analysis of multiplex PCRs that contain several PCR products For very small products, or for ... analyzed for expression levels within a day followed by selection for future heterologous expression strategies The in vitro expression of proteins can also be used for functional studies of proteins ... template, or a combination of such factors (Chapter 4) In such Analysis, sequencing and in vitro expression of PCR products 89 cases it is often possible to increase the sensitivity of the analysis in...

Ngày tải lên: 25/10/2013, 22:20

... mammalian cells [56], a decrease in the level of expression of Adxp has dramatic consequences for SCC activity Alterations in the level of expression of Adrp in yeast have no effect as described ... system in yeast is sensitive to the levels of expression of these components, similar to that found for mammalian adrenal cells [14] or for in vitro assay systems [55] In yeast, as in transfected ... levels of expression of various proteins (Adxp, Adrp, CYP11A1) involved in the reaction can also affect the efficiency of cholesterol side chain cleavage Indeed, it is apparent that the expression of...

Ngày tải lên: 08/03/2014, 08:20

... overview of catabolic pathways Metabolism of fatty acids Metabolism of glucose (glycolysis) Conversion of pyruvate to acetyl coenzyme A Anabolism of fatty acids from CH3COSCoA Anabolism of glucose ... Condensation of isoprene units Formation of acyclic terpenes -H+ OPP OPP OPP H OPP OPP geranyl OPP OPP farnesyl OPP OPP tail-tail dimerization OPP geranylgeranyl OPP squalene Formation of monoterpenes ... 3-carene Me α- and β-pinene Formation of sesquiterpenes (examples) Me Me bisabolene Me Me Me H Me Me cadinene OPP H Me Me Me Me Me Me OH Me carotol Me Me Formation of diterpenes (examples) Me...

Ngày tải lên: 18/03/2014, 20:20

... solution of 1% PFA in PBS For each donor, we included isotype controls for CD16, CD56 and NKp46 Cell surface expression level of NKp46 is expressed as ratio of the MFI (Mean Fluorescence Intensity) of ... and analysis software ImageJ software [28] was used to conduct as semi-quantitative analysis of expression Mean voxel intensity of bands was used to determine product expression of the reference ... for 2-5 sec and rinsed for Sections were dehydrated and placed in 70% methylated spirit for min, then 90% for min, then 100% for min, and again at 100% for Slides were then placed in xylene for...

Ngày tải lên: 19/06/2014, 22:20

... conditions for the multiplex-nested ARMSPCR were 95°C for followed by 35 cycles of denaturation at 95°C for min, annealing at 55°C for min, DNA extension at 72°C for and a final extension at 72°C for ... the local strains, we performed PCR with the two sets of primers and different combinations of templates, such as one of the vaccine strains with or without the presence of a field isolate The ... established on the basis of the genetic divergence spanning from the intergenic region of the M and F genes to the Fsp region of F gene The level of genetic variation of the F gene between the...

Ngày tải lên: 12/08/2014, 04:20

... Specification for Ethanol Fuel Blends for FlexibleFuel Automotive Spark-Ignition Engines D5842 Practice for Sampling and Handling of Fuels for Volatility Measurement D5949 Test Method for Pour Point of ... Petroleum Products (Automatic Pressure Pulsing Method) D5950 Test Method for Pour Point of Petroleum Products (Automatic Tilt Method) D5985 Test Method for Pour Point of Petroleum Products (Rotational ... D6300 Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants D6708 Practice for Statistical Assessment and Improvement of Expected Agreement...

Ngày tải lên: 20/10/2014, 17:17

... DEVELOPMENT OF LIVE BACTERIAL SYSTEMS FOR PRESENTATION OF DENGUE EDIII TO THE MUCOSAL IMMUNE SYSTEM LAM JIAN HANG B.SC (HONS), NUS A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... problems is crucial for the success of this subunit vaccine approach 1.5 LIVE BACTERIAL VECTORS FOR ANTIGEN DELIVERY 1.5.1 Rationale for using a live bacterial vector Live bacterial vectors that express ... SUITABILITY OF EDIII AS A SUBUNIT VACCINE CANDIDATE 4.2 146 BORDETELLA PERTUSSIS AS A LIVE VECTOR FOR THE PRODUCTION AND MUCOSAL DELIVERY OF DENV2 EDIII 4.3 147 LACTOCOCCUS LACTIS AS A LIVE VECTOR FOR...

Ngày tải lên: 09/09/2015, 08:18

... Application of ABPP for Target ID of Natural Products 17 1.2.5 Application of ABPP for Imaging of Protein Activities 20 Chapter 2: Activity-Based Proteome Profiling of Potential Cellular Targets 24 of ... 16 1.2.4 Application of ABPP for Target ID of Natural Products Natural products, and their derivatives and mimics, have rendered an enormous contribution to the treatment of diseases such as infentions ... of bioactive small molecules and natural products Several excellent reviews[5] have been published recently in the field of ABPP describing the variety of probes and breadth of applications of...

Ngày tải lên: 09/09/2015, 18:54

... unsuccessful but fortunately the supply of (+)‑discodermolide could be obtained through total synthesis.64,65 1.2.5 Synthesis of Natural Products The main problems of developing drugs from natural products ... of diseases, it is unlikely that there will ever be a single cure for cancer Today, natural products or their derivatives account for about half of the drugs that are used for the treatment of ... Microbes as Sources of Antitumor Agents 14 1.2.4 Anticancer Drugs from Marine Sources 16 1.2.5 Synthesis of Natural Products 17 1.2.6 Semisynthesis and Total Synthesis of Natural Products 18 1.2.7...

Ngày tải lên: 10/09/2015, 15:49

... effective components in natural products Hence, the major aim of this thesis is to expand the analytical applicability of CE in the analysis of natural products The range of natural products is quite ... have been used for the separation of different groups of alkaloids 1.3 Scope of the research Natural products are interesting to researchers because of their inestimable potential to offer a vast ... the development of pharmaceutical industry relies closely on the understanding of natural products Plenty of drugs, such as Penicillin, taxal, morphine, were developed from natural products The...

Ngày tải lên: 11/09/2015, 14:27

... staff of Department of Chemistry for NMR, MS and XRD analysis, and Ms Chua Ling Lih for the technical support for the biological part of my project I also want to thank National University of Singapore ... all of you, for your love, understanding and encouragement Contents Summary I List of Tables III List of Figures IV List of Schemes V Chapter General introduction 1.1 Introduction 1.2 Natural Products ... N H N O HO (42) CNS 2013 1.3 Biosynthesis of Natural Products There are a huge number of natural products in nature, and they differ greatly in terms of their structures, characteristics and functions...

Ngày tải lên: 03/10/2015, 20:33

... 8.67 — — NATURAL SYSTEMS FOR WASTEWATER TREATMENT TABLE Performances of some well known constructed wetlands 11/18/2005 10:44:18 AM NATURAL SYSTEMS FOR WASTEWATER TREATMENT Cost Cost is often a ... the type of treatment system for a particular application Unfortunately, the availability of reliable cost data for wetland treatment systems is limited The cost of wetland treatment systems varies ... 738 NATURAL SYSTEMS FOR WASTEWATER TREATMENT by pumping or under-drain systems for recovery of treated water, (3) natural treatment with groundwater moving...

Ngày tải lên: 10/08/2014, 20:20

... RAGE expression in FLS cultures in our experiments and may be relevant to the over -expression of RAGE on RA synovial tissues However, the exact mechanism of RAGE over -expression in the milieu of ... Elliston K, Stern D, Shaw A: Cloning and expression of a cell surface receptor for advanced glycosylation end products of proteins J Biol Chem 1992, 267:14998-15004 Hofmann MA, Drury S, Fu C, Qu W, Taguchi ... pathway of IL-17-induced RAGE production and expression, we tested the effect of Act-1 shRNA on RAGE production We produced Act-1 shRNA and confirmed the inhibitory effect of Act-1 shRNA on Act-1 expression...

Ngày tải lên: 12/08/2014, 17:22

... boundaries, etc.) Examples of open systems are: groundwater systems, social systems, as well as most of the natural systems Four types of variables characterize a model of a system (Kramer and De ... validation of numerical models of natural systems is impossible This is because the natural systems are never closed and model results are always non-unique The openness of natural systems is ... maximum values of the uncertainty range of factor xi, respectively The symbol p denotes the number of levels chosen for each factor For the sake of technical convenience, each element of vector X is...

Ngày tải lên: 06/11/2012, 10:35

... three main formats according to the informants One format of the interview guide is for officers from eight governmental agencies who are the subjects of the overall case The second format aims ... Office of the Basic Education Commission (OBEC); iv Office of the Non-formal and Informal Education (ONIE); v Vocational Education Commission (VEC); NSTDA (2008) Memorandum of understanding for ... is that the transformation to sustainable forms of energy in rural areas of developing countries may take time and need a lot of efforts Even if the energy is provided for free of charge, there...

Ngày tải lên: 05/09/2013, 16:10

... of the cable length by the instrument Notes on the use of hand-held test devices for installation of the KRONE Cat.6 KM8 products December 2001 Page of 23 IDEAL LT8600LT The IDEAL LT8600 performs ... on the determination of the cable length by the instrument Notes on the use of hand-held test devices for installation of the KRONE Cat.6 KM8 products December 2001 Page of 23 MICROTEST OMNIScanner2™ ... on the determination of the cable length by the instrument Notes on the use of hand-held test devices for installation of the KRONE Cat.6 KM8 products December 2001 Page of 23 Agilent WireScope...

Ngày tải lên: 21/12/2013, 18:15

... and the Institute of Medicine The members of the committee responsible for the report were chosen for their special competences and with regard for appropriate balance Support for this project ... president of the National Academy of Sciences The National Academy of Engineering was established in 1964, under the charter of the National Academy of Sciences, as a parallel organization of outstanding ... driven by the imperative of reliably providing short-term weather information, is itself a union of heretofore separate civilian and military programs It is a marriage of convenience to eliminate...

Ngày tải lên: 17/02/2014, 07:20

... operation The Michigan Department of Natural Resources (MI DNR) is responsible for the management of the Michigan state forestlands on an area of 1.6 Mio (about 21% of the state’s timberland) DNR ... the State Forest System are largely noncontiguous tracts of forest that are scattered throughout the landscapes of the Northern Lower Peninsula of Michigan and all of Upper Peninsula of Michigan ... Chain of Custody Surveillance Audit of SCS for 2006: Michigan DNR made up a substantial portion of the stand stocking There does not appear to be a concerted effort by DNR to manage only for aspen...

Ngày tải lên: 17/02/2014, 21:20

... potential for improving the performance of the system A simple case study was also recently conducted on the effects of saturations to MIMO systems where potential for improvement in the performance ... was described above, then x(t)e BA,C for all t and for all u(t) Proof: The proof of this Lemma follows from the construction of X(t) //// Page 13 Theorem 3.4: For the system (3.11)-(3.12) with X(t) ... control signals For multivariable systems, a major problem that arises (because of saturations) is the fact that control saturations alter the direction of the control vector For example, let...

Ngày tải lên: 18/02/2014, 10:20