using in vitro cell culture models to evaluate implant surfaces

... growth factor PCL; PCL/PLGA solvent evaporation In vitro Indomethacin PCL melt-dispersion In vitro Nitrofurantoin Insulin PCL PCL solvent evaporation solvent evaporation In vitro In vitro Gadzinowski ... PCL/2,2-bis(2oxazoline) Techniques Vitro/ Vivo Researchers In vitro In vitro Pitt et al.42; Buntner et al.43,44 Martini et al.45 Guerra et al.46 both Limin et al.47 In vitro Barbato et al.48 In vitro Cha ... toxin or pollutant) Recognition proteins include antibodies, avidin, streptavidin, Protein A, Protein G, cell membrane receptors (e.g., integrins), and many others.1–3 The process may be used in...

Ngày tải lên: 28/11/2015, 13:53

... conserved inverted repeat in the 3'-noncoding region of the S segment seems to be involved in recombi- nation events, leading, however, to the deletion of the hairpin-forming sequences (A Plyusnin, ... pre-passaged recombinant virus, however, presented no real match to the original cell adapted variant, TUL02, it terms of fitness Taking into account that the in situ formed recombinant S RNA disappeared ... weaker due to the fact that two non-canonical G:U base pairs presented in the plus-sense RNA occur as nonpairing C/A bases in the minus-sense RNA Interestingly, in Puumala hantavirus, a hairpin-like...

Ngày tải lên: 18/06/2014, 22:20

... conserved inverted repeat in the 3'-noncoding region of the S segment seems to be involved in recombi- nation events, leading, however, to the deletion of the hairpin-forming sequences (A Plyusnin, ... pre-passaged recombinant virus, however, presented no real match to the original cell adapted variant, TUL02, it terms of fitness Taking into account that the in situ formed recombinant S RNA disappeared ... weaker due to the fact that two non-canonical G:U base pairs presented in the plus-sense RNA occur as nonpairing C/A bases in the minus-sense RNA Interestingly, in Puumala hantavirus, a hairpin-like...

Ngày tải lên: 20/06/2014, 04:20

... temperature The cells were again washed and resuspended in ml saline and subjected to flowcytometry Cells collected using flow cytometry on a FACS were analyzed using FlowJo software by gating on the ... (n = 15) included in this study by density gradient centrifugation using the Ficoll Histopaque method BCG vaccine (Moscow Strain) was obtained from Serum Institute of India, Pune and stored at ... 4-8°C Prior to use, vaccine was reconstituted in sterile saline After counting, the cells were cultured in RPMI-1640 medium keeping the concentration at × 105 cells/well and were induced with...

Ngày tải lên: 11/08/2014, 08:21

... like to stop smoking if it was easy? YES †Not Sure _NO † _ NO Q13 If yes, are you intending to stop smoking within the next month? YES †NO † † YES Q12 Are you intending to stop smoking ... you try to stop smoking GOOD THINGS you enjoy it it's relaxing it stops stress it breaks up the day it relieves boredom it's sociable it stops weight gain it stops you craving BAD THINGS it's ... letters to show increased intention to quit (for example, say in their initial questionnaire that they did not intend to quit, but say in the followup questionnaire that they did intend to quit)...

Ngày tải lên: 23/03/2014, 19:20

... Effect of4 Figure inhibitors on in vitro processing Effect of inhibitors on in vitro processing Various concentrations of protease inhibitors were added to the in vitro processing assay for hr ... Effect of inhibitors on in vitro processing Inhibitor Name Concentration Inhibit Cleavage Metalloproteinase Aspartic acid proteinase Serine proteinase Cysteine proteinase EDTA Pepstatin PMSF E-64 ... serine protease inhibitor PMSF all failed to inhibit the cleavage reaction indicating that the enzyme responsible for cleavage is a cysteine protease Interestingly the cysteine protease inhibitors...

Ngày tải lên: 19/06/2014, 08:20

... Effect of4 Figure inhibitors on in vitro processing Effect of inhibitors on in vitro processing Various concentrations of protease inhibitors were added to the in vitro processing assay for hr ... Effect of inhibitors on in vitro processing Inhibitor Name Concentration Inhibit Cleavage Metalloproteinase Aspartic acid proteinase Serine proteinase Cysteine proteinase EDTA Pepstatin PMSF E-64 ... serine protease inhibitor PMSF all failed to inhibit the cleavage reaction indicating that the enzyme responsible for cleavage is a cysteine protease Interestingly the cysteine protease inhibitors...

Ngày tải lên: 20/06/2014, 04:20

... gas to produce coating with thickness ranging from 40-70 microns (nominal 50 microns) The coating had a minimum total crystallinity of 65% The minimum HA fraction in the crystalline phase was 90% ... coating may contribute more to osseointegration The differences in findings underscore the need for additional research to better understand the processes that influence osseointegration Osseointegration ... parameters may also be important in determining potential for osseointegration Osseointegration was only measured using one histomorphometric parameter (bone-toimplant contact) We did not measure...

Ngày tải lên: 20/06/2014, 04:20

... nanoparticles interfered with the intracellular trafficking of the MTT formazan vesicles in HeLa cells and astrocytes, resulting in an overestimation of cytotoxicity when compared to flow cytometry [11] In ... were compared to a calibration curve carried out using bovine serum albumin, and the protein concentration was determined.a Cell membrane integrity analysis Cells were seeded into a 96-well plate ... resulted in a similar increase in cellular uptake compared to the uncoated nanoparticles The cytotoxicity of the polymer-coated nanoparticles was determined using three commonly used cytotoxicity...

Ngày tải lên: 20/06/2014, 20:20

... nanoparticles interfered with the intracellular trafficking of the MTT formazan vesicles in HeLa cells and astrocytes, resulting in an overestimation of cytotoxicity when compared to flow cytometry [11] In ... were compared to a calibration curve carried out using bovine serum albumin, and the protein concentration was determined.a Cell membrane integrity analysis Cells were seeded into a 96-well plate ... resulted in a similar increase in cellular uptake compared to the uncoated nanoparticles The cytotoxicity of the polymer-coated nanoparticles was determined using three commonly used cytotoxicity...

Ngày tải lên: 21/06/2014, 17:20

... pattern, juvenile potentialities during a period that shortens with increasing ontogenic development, the maximum intensity corresponding to bud-break This opinion is in total accordance with Krenke’s ... potentialities, including rooting abilities, as the juvenile clone This rejuvenation has been maintained for more than yr for in vitro as well as for outdoor cultivated rooted cuttings In addition, ... 1987) Taking into account this physiological parameter susceptible of fluctuating greatly under outdoor conditions (Monteuuis and Gen- to be determinant in successful meristem culture of ensuring...

Ngày tải lên: 09/08/2014, 02:21

... scaling exponent for skin, 0.96 The scaling exponent for the mass of the gastrointestinal tract is also close to However, histological examination reveals a metabolically active layer of cells ... References As explained in the PVFCP model, pressure in capillaries necessarily falls as the rate of blood flow in a tissue decreases (assuming that the level of constriction in veins draining the tissue ... midpoint of the values of bi because values of ki are not available for certain tissues For scaling of the brain and the epithelial tissues of the skin and gastrointestinal tract, the midpoint...

Ngày tải lên: 13/08/2014, 23:20

... ta th tính u n s phát sinh hình thái c a mô nuôi c y, ng b sung vào môi ng nuôi c y nhóm ch u ti t sinh tr ng th c v t auxin cytokinin N u t l auxin/cytokinin th p s phát sinh hình thái theo h ... Lin CM (1981) Genotypic differences in plant production in anther culture of rice In: Chang WC (ed) Proc Symp Plant cell tissue culture Acad Sin, Taipei, pp 199-203 10 Chen CC, Lin MH (1976) Induction ... application in crop improvement In: H Han and H.Y Yang (Eds.) , Haploids of Higher Plants In Vitro, China Academic Publishers, Beijing/Springer-Verlag, Berlin, pp 118136 14 Proceedings of the...

Ngày tải lên: 24/11/2014, 01:08

... Strain -to- strain variation in Blastocystis induced drop in Caco-2 TER 4.3.2 Blastocystis ST-7 (B) induces an increase in Caco-2 epithelial permeability to FITC conjugated Dextran 4.3.3 Blastocystis ... and Infection, June 18th-21st 2009, Toronto, Canada xxii Haris Mirza & Kevin SW Tan Intestinal epithelial barrier disruption by Blastocystis subtype-7 in an in vitro model 2nd International Singapore ... intestinal and non-intestinal symptoms are associated with Blastocystis infections 1.6.1 Intestinal symptoms Blastocystosis frequently presents with diarrhea and abdominal pain (Tan, 2008; Tan...

Ngày tải lên: 10/09/2015, 15:53

... NF-κB in intestinal epithelial T84 cells following exposure to Blastocystis ratti WR1 Fig 6.8 Representative micrographs showing nuclear translocation 159 of NF-κB in intestinal epithelial HT29 cells ... human secretory immunoglobulin A (SIgA) by the intestinal protozoan Blastocystis Research work presented in Combined Scientific Meeting 2005, Singapore xviii CHAPTER 1: INTRODUCTION 1.1 INTRODUCTION ... it to as Blastocystis infections Clinical symptoms attributed to Blastocystis infections include recurrent watery diarrhea, mucous diarrhea, vomiting, abdominal cramps and flatulence Blastocystis...

Ngày tải lên: 11/09/2015, 09:11

... possible induction of cell stress in the skin by WÆkg)1 GSM-900 exposure for 48 h No apoptosis was induced in either skin cell type, in agreement with reports of other in vitro studies concluding ... corresponding to WÆkg)1 in the medium under GSM-900 protocol (1 ⁄ time slot) was 16 WÆkg)1 Exposure took place at 37 ± 0.1 °C with continuous temperature recording in the incubator A twin incubator ... GSM-900 and cell stress in skin models S Sanchez et al expression under stress conditions has been reported in a number of cell types, including skin cells The major HSPs expressed in the skin [5]...

Ngày tải lên: 07/03/2014, 11:20

... culture of infected hepatocytes To succeed in obtaining HCV infection in primary human hepatocytes, an existing model of hepatitis B virus infection was used to determine optimal infection conditions ... al In vitro HCV infection models mutations Furthermore, it should be kept in mind that, in this system, hundreds of RNA copies per cell are present, in contrast to 5–50 copies in infected hepatocytes ... ascertained using systems closer to HCV physiology Infection of primary cell cultures and cell lines Parallel to the development of this replication system, intensive research has aimed to discover...

Ngày tải lên: 16/03/2014, 05:20

... proteins including CD59, clusterin, vitronectin, C1-inhibitor, C4-binding protein, decay-activating factor, and Factor H, which inhibit different steps in the complement cascade All of these regulatory ... proteins, including in vitro protection against excitotoxicity [51,52] and Aβ-induced neurotoxicity [53], as well as anti-apop- totic effects [54,55] Further, C1q, the first complement protein to ... systemic administration of the excitotoxin kainic acid [108,109], or injection of the neurotoxin colchicine into the dorsal hippocampus [109] Surgical transection of the perforant pathway increased...

Ngày tải lên: 19/06/2014, 22:20

... during the culture Next, we wanted to see if either proinflammatory histone deacetylase inhibitor TSA or anti-inflammatory NF-κB inhibitor helenalin could influence the LPS-induced response Interestingly, ... dying cells and living cells, we used standard Nissl staining and the Live/dead-cytotoxicity kit L-3224 (Molecular Probes), respectively, according to the manufacturer's protocol LDH leakage to ... importance to pay attention to the factors that contribute to the consistency of in vitro models used to mimic in vivo situations In the present study, we used hippocampus tissue slices in serum-free culture...

Ngày tải lên: 19/06/2014, 22:20

... IFN-inhibiting domains in the P and V proteins of Location of IFN-inhibiting domains in the P and V proteins of MeV P indicates P protein, V indicates V protein, NTD indicates shared amino terminal ... (indicator cells) was transfected with μg pG1NT7 using TransIT-LT1 (Mirus Bio) 18 hours after transfection, indicator cells were detached with trypsin (Invitrogen) and added to the wells containing ... Maisner A: A single amino acid change in the cytoplasmic domains of measles virus glycoproteins H and F alters targeting, endocytosis, and cell fusion in polarized Madin-Darby canine kidney cells J...

Ngày tải lên: 20/06/2014, 01:20