... processes of heat treatment and kieselguhr filtration MF has the advantages of being simple to perform, of maintaining the chemical balance of the brine and of eliminating filter aids In the brine treatment ... is one of the largest water-using industries In the industry, water is used as an ingredient, for initial and intermediate cleaning of the product, and as a key agent in the sanitation of the ... bovine serum albumin, immunoglobulins, lactoferrin, transferrin, and some minor proteins and peptides (see also Table 2.2) For example, b-lactoglobulin can be used in emulsification, foaming and...
Ngày tải lên: 02/04/2014, 15:04
... region of 35 kDa (Fig 4B, right) This finding is in agreement with the lack of incorporation of ISP into the 500 kDa bc1 sub-complex and clearly underlines the importance of Qcr9p for ISP binding ... that Qcr6p and Qcr9p are not required for Bcs1p binding and that the binding of ISP and Qcr10p is subsequent to that of Bcs1p Previous studies have suggested that the insertion of ISP into the ... protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Dulley JR & Grieve PA (1975) A simple technique for eliminating interference by detergents in the Lowry method of...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: N-terminal extension of the yeast IA3 aspartic proteinase inhibitor relaxes the strict intrinsic selectivity ppt
... saccharopepsin, the validity of this interpretation was examined by determination of inhibition constants for the interaction of the N-terminally extended inhibitors with saccharopepsin The potencies of ... Changes in other locations, including the ‘remote’ attachment of residues 35–81 from its own C-terminal segment, cause only minor perturbation of the inhibitory potency intrinsic to the N-terminal ... important in restricting the activity of S cerevisiae IA3 to saccharopepsin as its sole target proteinase were introduced into the S castellii sequence in place of the intrinsic M18 ⁄ K22 pair, the inhibitory...
Ngày tải lên: 19/02/2014, 00:20
Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf
... 100 Interestingly, the absence of subunit also resulted in an increase in the ratio of intermediate to mature cytochrome c1 and a disappearance of the intermediate form of the Rieske protein At ... detectable in the CKL57 strain We not know whether the lack of immunoreactivity in the latter strain was due to the inability of the truncated protein to insert into and be stable in the inner mitochondrial ... Core and core proteins were significantly reduced in both mutant strains, being 33 and 29%, respectively, in the W303–1B q° strain (Fig 4A,C) and 57 and 53%, respectively, in the CKL57 strain (Fig...
Ngày tải lên: 19/02/2014, 12:20
Báo cáo khoa học: Observation of a chaotic multioscillatory metabolic attractor by real-time monitoring of a yeast continuous culture doc
... mechanism in the induction and maintenance of oscillations in continuous cultures of budding yeast Biotechn Bioeng 36, 453–459 16 Ghosh AK, Chance B & Pye EK (1971) Metabolic coupling and synchronization ... amount of information about point i + k we have if we know point i [46] The mutual information curve is shown in Fig The oscillations in the mutual information are due to the strong 4-min mode of ... period of 19.1 ± 0.9 points, or 3.82 ± 0.19 Construction of a time-delay embedding requires both a delay and an embedding dimension The first minimum in the mutual information curve was used to...
Ngày tải lên: 07/03/2014, 10:20
Báo cáo khoa học: Structure of the complex of a yeast glucoamylase with acarbose reveals the presence of a raw starch binding site on the catalytic domain doc
... mutations of the amino acids proposed to be involved in binding of the surface acarbose caused reduction of enzyme adsorption on starch, proving that these amino acids are involved in starch binding ... remote ligand binding site Fig A space filling model showing the complex of glucoamylase with acarbose Both acarbose molecules are in yellow Tyr464 is in green, Asp450 in red and Arg15 in blue The ... side-chains The structure of the starch binding domain of A niger glucoamylase was solved by NMR in its native state [10] and in a complex with b-cyclodextrin [11] Crystal structures of an intact...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Characterization of recombinant forms of the yeast Gas1 protein and identification of residues essential for glucanosyltransferase activity and folding pot
... multidomain proteins [28], or for the sequential and independent folding of the single domains – assuming a vectorial mode of folding for Gas1p In any case, the replacement of C74 with a serine could ... glycosylation profile of the proteins was examined Aliquots of medium containing the recombinant proteins were treated with Endo H and analysed by Western blotting using anti-Gas1p immunoglobulin (Fig 2C) ... transferase domain (GluTD), a cystine-rich region containing a motif of eight cysteines (C370–C462) and a serine-rich region in which 28 serines are clustered in a region between residues S485 and S525...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: The GxxxG motif of the transmembrane domain of subunit e is involved in the dimerization/oligomerization of the yeast ATP synthase complex in the mitochondrial membrane doc
... G19L and C28S were introduced by a PCR mutagenesis procedure [24] into a plasmid pRS313 bearing an insert encoding the wild TIM11 gene and the kanr gene The strains containing modified versions of ... EMBL) and Saccharomyces cerevisiae (P81449, Swiss-Prot) are in bold The numbering of the yeast subunit e begins at the initiating methionine The star indicates the position of the unique cysteine ... devoid of Cys28 (not shown), thus indicating the involvement of Cys28 in the formation of the adduct The 21.4-kDa band corresponded to a homodimer of subunit e resulting from the formation of a...
Ngày tải lên: 31/03/2014, 01:20
Báo cáo Y học: Interaction of the GTS1 gene product with glyceraldehyde3-phosphate dehydrogenase 1 required for the maintenance of the metabolic oscillations of the yeast Saccharomyces cerevisiae pdf
... Determination of the binding site of Gts1p in the 97 C-terminal residues of GAPDH3 (GAPDH3-C97) using N-terminal and C-terminal deletion mutants (A), and mutants with modifications in the zinc finger ... genes indicate DNAs inserted in- frame downstream of the DNA-binding domain of GAL4 For prey, a cDNA encoding GAPDH3-C97 inserted downstream of the activation domain of GAL4 was used Open boxes and ... the binding sites were located in both the N-terminal and C-terminal portions of Gts1p, as truncation of either portion resulted in a significant loss of binding activity (Fig 1A) Keeping in mind...
Ngày tải lên: 31/03/2014, 21:21
cadaver dog handbook forensic training and tactics for the recovery of human remains
... recent increase in interest and demand for training and handling information, as well as for standardization and professionalization of training and handling methods The misapplication and incorrect ... Guidelines for Training and Practice Training Scenarios Ongoing Training Regime Frequently Asked Questions Final Note and Caution CHAPTER Training Materials Creating and Handling Training Aids Scent ... of understanding scent, canine olfaction, and the ABC’s of training and training equipment Chapters and are focused on the forensic context, including the demands of professionalism and the legal...
Ngày tải lên: 03/06/2014, 01:36
state university of new york press adorno the recovery of experience oct 2007
... understanding of conceptual life Diamond illustrates this in terms of a contrast between grasping a concept in the sense of “knowing how to group things under that concept,” and in the sense of “being ... Bergson in Adorno scholarship is, I believe, one of the main reasons why the idea of spiritual experience has remained undiscovered The understanding of conceptual thinking as a form of domination ... idea of giving voice to suffering in terms of a destruction of the illusory, ideological claim of unity of thought and thing The claim of such a unity is ideological, Adorno believes, because...
Ngày tải lên: 11/06/2014, 12:47
differentiation of brewing yeast strains by pyrolysis
... ation of brewery yeast strains by DNA fingerprinting J Inst Brewing 101, 75–78 Timmins, E M and Goodacre, R (1997) Rapid quanu titative analysis of binary mixtures of Escherichia coli strains using ... brewing strains of yeast by DNA fingerprinting Lett Appl Microbiol 22, 90–94 Windig, W., Haverkamp, J and Kistemaker, P G (1983) Interpretation of sets of pyrolysis mass spectra by discriminant analysis ... between strains was very dependent on the restriction enzyme used, and no enzyme was successful in causing obvious banding for differentiating lager strains from ale strains This genotypic analysis...
Ngày tải lên: 12/06/2014, 11:18
báo cáo hóa học: " Recovery of visual fields in brain-lesioned patients by reaction perimetry treatment" potx
... VF size of – 7° Reading training had a similar effect: In 34% of hemianopic patients who participated in reading training, an average VF enlargement of 5.4° was obtained after 15–24 training sessions, ... Activities of daily living The ability to perform visual related activities of daily living (ADL) such as reading, avoiding obstacles, orienting in space, walking, riding a bike, manipulating with things ... = 13) of patients were treated binocularly since that type of treatment was reported to be less exhausting with respect to maintaining fixation and keeping attention than monocular training To...
Ngày tải lên: 19/06/2014, 10:20
báo cáo hóa học: " Apolipoprotein E isoform-dependent dendritic recovery of hippocampal neurons following activation of innate immunity" pot
... brain injury, and HIV-encephalitis [1] Confounding a clear interpretation of the contribution of innate immune activation to neurodegeneration in human diseases and their corresponding animal models ... some bacteria and viruses [34] It also modulates inflammation in cell culture and in vivo models of brain injury, where an apoE mimetic therapeutic peptide has been shown to reduce CNS inflammation ... DT, Colton CA, Vitek MP: Apolipoprotein E-derived peptides ameliorate clinical disability and inflammatory infiltrates into the spinal cord in a murine model of multiple sclerosis J Pharmacol Exp...
Ngày tải lên: 19/06/2014, 22:20
Báo cáo hóa học: " Recovery of divergent avian bornaviruses from cases of proventricular dilatation disease: Identification of a candidate etiologic agent" pptx
... the presence of ABV and clinically confirmed PDD in follow-up blinded PCR screening of a set of additional PDD cases and controls, with ABV was detected in 71% of PDD cases and none of the controls ... recovery, and drafting the manuscript, KF participated in array analysis, developed pipeline for ultra high throughput sequence analysis, and participated in design of filtering and iterative BLAST analysis, ... assisted in the selection of the PDD case/control specimens from Florida and participated in review of clinical and histological status of cases and controls included in the study, DG and JLD...
Ngày tải lên: 20/06/2014, 01:20
Báo cáo hóa học: " Blind recovery of k/n rate convolutional encoders in a noisy environment" pdf
... optimal encoders used in the following section dedicated to the analysis and performances study of our blind identification method Analysis and performances In order to gain more insight into the performances ... triangular matrix (Gl) through use of the Gauss Jordan Elimination Through Pivoting adapted to GF(2): Gl = Al Rl Bl (23) where Al is a row-permutation matrix of size (L × L) and Bl is a matrix of size ... deficiency of the matrix, Rna, is such that ≤ Z(na ) ≤ (n − k) Page of 16 G Sicot, S Houcke, Theoretical study of the performance of a blind interleaver estimator, in Proceedings of the ISIVC,...
Ngày tải lên: 20/06/2014, 22:20
Báo cáo hóa học: " Research Article Recovery of Myocardial Kinematic Function without the Time History of External Loads" ppt
... requirement of complexity and accuracy of the model by introducing uncertainties into the biomechanical model In the following subsections, a deterministic the finite-element representation of the ... by using the MRI-SPAMM tagging technique and a deformable model from images Inspired by the work [33] of input estimation in the inverse heating problem without the time history of external input, ... computation and estimate 3D myocardial kinematic function using a small number of principal modal components in [25] In spite of the potential advantage of computational speed in [25], the effect of infarction...
Ngày tải lên: 21/06/2014, 19:20
Báo cáo hóa học: " Research Article A Sharing-Based Fragile Watermarking Method for Authentication and Self-Recovery of Image Tampering" potx
... coherence time Tcoh , and is statistically independent of the fading gain in other intervals of duration Tcoh During an interval Tcoh , several packets are transmitted, as indicated in Figure Packets ... gains γARQ of (max retransmission) and (max retransmissions), respectively In the case of RS erasure coding, overheads of 10%, 20%, and 40% yield diversity gains γRS of (i.e., no diversity gain), ... exploited to increase the PHY layer diversity by means of frequency-interleaving and coding across the subcarriers of an OFDM block) The detailed analysis of this case is beyond the scope of this...
Ngày tải lên: 21/06/2014, 22:20
Báo cáo hóa học: " Research Article A Simplified Constant Modulus Algorithm for Blind Recovery of MIMO QAM and PSK Signals: A Criterion with Convergence Analysis" ppt
... or imaginary part), as opposed to CMA The major advantage of SCMA is its low complexity compared to that of CMA and MMA Because, instead of using both real and imaginary parts as in CMA and MMA, ... proposed [18–20] This algorithm, instead of minimizing the dispersion of the magnitude of the equalizer output, minimizes the dispersion of the real and imaginary parts separately; hence the ... purpose, we use the signal to interference and noise ratio (SINR) defined as gkk SINRk = , =k g k T ∗ + wk R b wk , (74) M SINRk , SINR = M k=1 where SINRk is the signal-to-interference and noise...
Ngày tải lên: 22/06/2014, 06:20
Báo cáo nghiên cứu khoa học: "USE OF IMMOBILIZED YEAST CELL IN ALCOHOL FERMENTATION FROM MOLASSES" ppsx
... 1.5381 and b is 6.0895 Using the slope and intercept function of Microsoft Excel to determine a and b, and RSQ function determines R2 = 0.9866 Note: The test samples were diluted by 103 Table 3.2 and ... are softer and more disintegrative than Alco3.0% The evolution of carbon dioxide causes an internal mechanical loading on the beads, which led to disintegration of most of these calcium alginate ... mass of free yeast and Alco3.0% Fermentative opt pH and opt t0 of free yeast and Alco3.0% are similar that are pH = and t = 300C 3.5.Determination of optimal fermentable time of free yeast and...
Ngày tải lên: 22/07/2014, 03:20