... respectively They are deduced in accordance with the maximal mAb heparin effect on thefunctional activity of TC Arrows show the hypothetical movement ofthe domains after attachment of Cbl, see the ... None ofthe other antibodies recognized the above peptides The smaller synthetic fragments (c, d, e) imitated other segments ofthe CNBr peptide in Fig 4A, but did not inhibit mAb binding to the ... either loss ofthe epitope or an artifact ofthe blotting procedure The location of peptide 1011 along the primary structure of TC is shown in Fig 4A as sequence with a blue solid underline The...
... challenging than for the other oligosaccharides, due to the poor dispersion of signals in the 3.2–4.1 p.p.m region ofthe spectra Although the 1H signals ofthe anomeric protons ofthe 2951lgt5 ⁄ 4D ... to obtain the 13C shift ofthe C1 and C2 resonances of Kdo Examination ofthe 400 ms NOESY spectrum confirmed each ofthe glycosidic linkages for the 2951lgt1 ⁄ 4D oligosaccharide and the terminal ... elucidate the 13C shift ofthe C1 and C2 resonance of Kdo For the highly branched, central a-d-Glc residue C, the locations ofthe H3 ⁄ C3 and H5 ⁄ C5 correlations were conspicuous in the 1H-13C-HSQC...
... hydrolysis of ATP to adenosine triphosphate (ADP) alters the conformation ofthe head ofthe cross-bridge and produces energy for the pulling movement ofthe actin filament towards the centre ofthe ... dynamic NFκB recognition site The study was focused on the dynamics ofthe DNA, rather than the dynamics ofthe C-Rel protein during binding[99] However, the effects of protein dynamics in cell ... promoting the binding of HIV-1 to chemokine receptors on the host cell, such as CCR5 or CXCR4 This activates the gp41 protein and promotes the fusion ofthe HIV outer membrane with the host cell, thereby...
... from their homeland The results of these investigations will be expected to guide the researcher to the next goal ofthe research, which is to examine if there is a presence and existence of a ... couple of these arenas was not possible due to their exclusivity to Malaysian Chinese and also the unwillingness ofthe informants to lead the researcher into these sites While many ofthe informants ... primarily because of their claim of detachment from other Malaysians and that they are not very pre-occupied with the experiential reality of moving to another nation to live and work: The other Malaysian...
... allele and marker, the size ofthe effect ofthe mutant allele on the phenotype, the type of study population, and the penetrances ofthefunctional locus genotypes [23] Furthermore, the power calculations ... to determine the risk of disease before the onset of symptoms would be potentially of great benefit in asthma Theunderstandingof asthma pathophysiology might then enter the realm of clinical ... affect the expression ofthe phenotype Genetic polymorphism arises from mutation Different classes of polymorphism are generally named on the basis ofthe type of mutation from which they result The...
... the C-terminal domain of apoCII interacts with the interface ofthe N- and C-terminal domains of LPL and part ofthe lid surface [32] Because thefunctionalimportanceof LPL’s C-terminal domain ... illustrates the overall saddle shape ofthe dimeric structure and the key heparin-binding residues in the N-terminal domain held coordinately by the two subunits on the top ofthe hollow (Fig 1A) The ... established Of two possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies ofthe chimeric proteins of hepatic lipase and LPL and the tandem repeat approach of LPL...
... indicated by the MD simulations ofthe modelled ternary hGK–Glc– ATP complex In the final structure ofthe simulations, the interactions ofthe adenosine moiety were similar to those observed in the binary ... related to the question of whether ATP binds to the Glc-free enzyme and the possibility of a nucleotide- triggered change in protein conformation In this work, we have studied the interaction of ATP ... Fig The Glc binding isotherm for the mutant L146R GST–hGK The enhancement of ITF was measured at 25 °C with increasing concentrations of Glc in the absence (d) and presence (s) of 2.5 mM ATPcS The...
... isolated from human liver Furthermore, we investigated the speci®city ofthehuman CBG with respect to the glycone and aglycone moieties, and in particular characterized the ef®ciency ofthe enzyme ... derivatives The enzyme catalysed the release of 4NP from six ofthe 11 4-substituted substrates tested and the kinetic parameters for these are presented in Table We detected no measureable release of ... inhibitor of reCBG [the hydrolysis of 4NP-b-Dglucopyranoside (10 mM) was reduced by 98% in the presence of 4NP-b-D-mannopyranoside (10 mM)] Hydrolysis of xenobiotic glycosides by reCBG The ability of...
... subtypes of TPS system have been identified, which differ by the sequences ofthe TPS domains ofthe TpsA proteins and by those of their TpsB transporters [16,18] Nevertheless, the structure ofthe ... to be functionally relevant for other members ofthe superfamily Results Conservation of L6 sequence in the superfamily The VRGY motif is located at the tip of L6 of FhaC, which reaches the periplasm ... 1) Furthermore, the analysis ofthe sequences of a large number of predicted Toc75, Sam50 and Omp85 homologues indicated the presence of a closely related tetrad in the vast majority of these...
... different parts ofthe world These reports showed that nucleotide sequence differences between the target region ofthe respective genes and the primer sequences used for typing led to the genotyping ... if there was any mismatch between them Four mismatches were found in the Das G1 primer, 9T1-1, at the 5' end (Fig 2) Due to these mismatches, the Das G1 primer failed to detect most (75%) ofthe ... Gouvea primer, aBT1 Figure Nucleotide mismatches in the primers Nucleotide mismatches in the primers The target sequence is the VP7 gene of G1 Bangladeshi strains The G1 rotavirus VP7 gene specific...
... different parts ofthe world These reports showed that nucleotide sequence differences between the target region ofthe respective genes and the primer sequences used for typing led to the genotyping ... if there was any mismatch between them Four mismatches were found in the Das G1 primer, 9T1-1, at the 5' end (Fig 2) Due to these mismatches, the Das G1 primer failed to detect most (75%) ofthe ... Gouvea primer, aBT1 Figure Nucleotide mismatches in the primers Nucleotide mismatches in the primers The target sequence is the VP7 gene of G1 Bangladeshi strains The G1 rotavirus VP7 gene specific...
... during the process ofthe study The study was approved by the ethical committee ofthe participating institutions, and informed consent was obtained from patients and controls The ages of patients ... severity of CDH was defined from mild instability ofthe femoral head with slight capsular laxity, to moderate lateral displacement ofthe femoral head, without loss of contact ofthe head with the ... The differences in the clinical information between the genotypes were tested using the Mann-Whitney test, the Kruskal-Wallis test and the chi-squared test The linear trend of severity was analysed...
... analysis includes the estimation ofnucleotide diversity, the assessment of linkage disequilibrium structure (LD) and/or the correlation between polymorphisms and the evaluation of selection processes ... collection ofthe genome-wide expected pattern of SNPs To determine whether the effect of interlocus variance (gene sampling) may distort thenucleotide diversity estimates (θW & πT), we re-analyzed the ... and the complete set of accessions pooled together (51 alleles) The diversity estimates (πT and θW) displayed the same pattern independently the loci surveyed aNucleotide polymorphism and nucleotide...
... significant potential to elucidate these individual influences and their interactions Better understandingof these human and non -human elements ofthe system of care ofthe critically ill patient is ... moment, the fundamental resources and systems needed for the care ofthe critically ill patient are (a) staff, (b) the ways that they interact with each other, and (c) the non -human elements of systems ... the study ofthe function of people and teams at a detailed level is somewhat location-specific, and studies need to be scrutinised for transferable lessons Many ofthe themes identified in the...
... compared the proteomes of all three cell types In this study we compare the proteomes of mDCs, moDCs and KG-1 cells, and then attempt to relate this to thefunctional properties of these cells ... analyze the comparability of gels, the densities of spots matched in all three gels were plotted and a correlation coefficient value was calculated The proteome of mDCs showed different levels of ... Table shows the corresponding protein data The factor of difference was calculated by dividing the intensity ofthe protein spot in mDCs by that ofthe corresponding spot in moDCs or KG-1 cells...
... likelihood of distant recurrence The peak risk of recurrence occurs within the first three years after initial treatment ofthe disease with the majority of deaths occurring in the first years On the ... key features ofthe tumor can be identified by studying the bulk ofthe cells that make up the tumor mass The behavior ofthe cancer cells is affected by intrinsic (e.g., levels of transcription ... to a variable extent depending on the growth pattern and synthesis activity ofthe parent cell of origin There is a functional resemblance to the parent tissue The neoplastic cell population is...
... proliferation of NIH 3T3 cells (Aktas et al., 1997) Theimportanceofthe Ras-Raf-MEK-ERK pathway in the regulation of cyclin D1 is undisputed, but some other studies also highlight the requirement of other ... knowledge ofthe regulation of growth and death in cancer cells has expanded enormously This leads to a better understandingof how these chemotherapeutic agents work and change the face of drug ... and before the onset of DNA synthesis Many of these DE genes require de novo protein synthesis for their expression, suggesting that they are regulated by IE genes However, at present there are...
... using MCode commands [39] The speed ofthe stretcher motor was set to mm/s The speed ofthe XYstage was calculated from the speed ofthe stretcher, the stretch amplitude, and the jog distance in x-axis, ... 2.14 The scanning path ofthe substrate The inner part ofthe substrate is 10x10 mm2 However, because ofthe objective geometry and the clamping, the area available for imaging is 7x8 mm2 The ... that the same region of a sample remained in the field of view ofthe camera The user-defined microstep resolution (number of steps that complete one full motor rotation; denoted as “µst”) of the...
... and then ate all ofthe chicken eggs After all the eggs were gone they caught the young girl and left the house for the mountains There was no one in the village because everyone was at the island ... was still practised The monkeys screamed and made lots of noise because they were happy that they had caught the little girl and then they went to the forest The hair ofthe young girl kept getting ... bother us or to eat our crops’ but the monkeys continued to approach her until they reached the house They entered the house, and the young girl began beating them with coals from the fire but the...