transforming growth factorβ1 gene
Báo cáo y học: "Adverse effects of adenovirus-mediated gene transfer of human transforming growth factor beta 1 into rabbit knees" potx
... clinicalapplications of transforming growth factor-beta (TGF-beta). Growth Factors 1993, 8:1-9.3. Assoian RK, Komoriya A, Meyers CA, Miller DM, Sporn MB: Transforming growth factor-beta in human ... sequence analysis of simian transforming growth factor-beta cDNA. DNA 1987, 6:239-244.5. Miller DA, Pelton RW, Derynck R, Moses HL: Transforming growth factor-beta. A family of growth regulatory peptides.Ann ... trans-forming growth factor.Arthritis Research & Therapy Vol 5 No 3 Mi et al.Research articleAdverse effects of adenovirus-mediated gene transfer of human transforming growth factor beta...
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Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf
... Weber, P.C. (1996) Effects of dif-ferent polyunsaturated fatty acids o n growth- related early gene expression and cell growth. Lipids 31 (Suppl. ), S37–S40.7. Sinclair, A., Attar-Bashi, N. & ... reaction (RT-PCR): a sensitive method to examinebasic fibroblast growth factor-induced expression of th e early growth response gene- 1 (egr-1) in human umbilical arterialendothelial cells. Mol. ... receptorgamma1 gene expression in human aortic smooth muscle c ells.J. Biol. Chem. 277, 26808–26814.27. Yan, Y.X., Nakagawa, H., Lee, M.H. & Rustgi, A.K. (1997) Transforming growth factor-alpha...
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Tài liệu Báo cáo khoa học:Insulin-like growth factor 1 signaling regulates cytosolic sialidase Neu2 expression during myoblast differentiation and hypertrophy doc
... constitutively active form of AKT; DM, differentiating medium; GM, growth medium; IGF-1, insulin-like growth factor 1; IGFBP5, insulin-like growth factor 1-binding protein; IRS-1, insulin receptor substrate ... roleprimarily during embryogenesis and regeneration[28,29], IGF-1 has been reported to be essential formuscle differentiation and hypertrophy [1–3]. Unlikeother growth factors, IGF-1 is able ... C2 myoblasts overexpressingthe insulin-like growth factor 1 binding protein 5 and therefore notresponding to endogenously produced insulin-like growth factor 1.Although Neu2-transfected myoblasts...
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Báo cáo khoa học: Control of transforming growth factor b signal transduction by small GTPases pot
... signaling during transforming growth factor-beta-induced smooth muscle differentiation. J BiolChem 281, 1765–1770.143 Deaton RA, Su C, Valencia TG & Grant SR (2005) Transforming growth factor-beta1-induced ... localization of transforming growth factor-beta in idiopathic pulmonary fibrosis. Am J Respir CellMol Biol 5, 155–162.153 Broekelmann TJ, Limper AH, Colby TV & McDonaldJA (1991) Transforming growth ... essential for transforming growth factor-beta-mediated stress fiber formation. J Biol Chem 276,15362–15368.159 Engel ME, Datta PK & Moses HL (1998) RhoB isstabilized by transforming growth factor...
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Báo cáo khoa học: Insulin like growth factor-1-induced phosphorylation and altered distribution of tuberous sclerosis complex (TSC)1⁄TSC2 in C2C12 myotubes pptx
... or TSC2 have higher mTOR activity, result-ing in a robust cell growth and tumorigenesis[9,10,17,18]. Although it is clear that growth factor-induced activation of Akt blocks TSC1 ⁄ TSC2 inhibi-tion ... control cell growth and sur-vival. Cell 115, 577–590.15 Inoki K, Li Y, Xu T & Guan KL (2003) Rheb GTPaseis a direct target of TSC2 GAP activity and regulatesmTOR signaling. Genes Dev 17, ... during normal Drosophila development. Genes Dev18, 2479–2484.21 Huang J & Manning BD (2008) The TSC1-TSC2 com-plex: a molecular switchboard controlling cell growth. Biochem J 412, 179–190.22...
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Báo cáo Y học: Early growth response-1 gene (Egr-1 ) promoter induction by ionizing radiation in U87 malignant glioma cells in vitro pot
... Site-directed mutagenesisPlasmid pD7egrGFL was generated by exchange of đvenucleotides in CRE2 (nucleotides )71 to )58) fromACGTC t o CTCAT by site directed mutagenesis u sing akit (Stratagene). The ... many downstream genesand thus r egulation of their expression. Target genes of theEgr-1 transcription factor are numerous and include growth factors such as platelet derived growth factor A ... hans-peter.rodemann@uni-tuebingen.deAbbreviations: Egr-1, early growth response-1 gene; PDGF, plateletderived growth factor; bFGF, basic đbroblast growth factor; SRE,serum response element; MAPK, mitogen...
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Báo cáo khoa học: Fragile X-related protein FXR1 controls posttranscriptional suppression of lipopolysaccharide-induced tumour necrosis factor-a production by transforming growth factor-b1 doc
... anti-inflammatorycytokine transforming growth factor-b1 (TGF-b1) ofthe proinflammatory cytokine TNF-a via the inductionof FXR1 is the focus of this article.TGF-b1, a member of the large transforming growth factor-b ... 3Â-UTR contains AU-rich elements(AREs). AREs, which are found in many cytokine,inflammatory gene and oncogene mRNAs, are targetsfor binding proteins that regulate mRNA stability [14].Mice with ... cachectin.Science 234, 470–474.7 Parrillo JE (1993) Pathogenetic mechanisms of septicshock. N Engl J Med 328, 1471–1477.8 Cohen J (2002) The immunopathogenesis of sepsis.Nature 420, 885–891.9 Weil...
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Báo cáo y học: Identification of an element within the promoter of human selenoprotein P responsive to transforming growth factor-b ppt
... arylhydrocarbonreceptor expression by transforming growth factor-b1. Mol.Pharmacol. 59, 716 724.22. Doăhr, O. & Abel, J. (1997) Transforming growth factor-beta 1coregulates mRNA expression ... of a Smad bindingelement (SBE) in the JunB promoter that acts as a transforming growth factor-beta, activin, and bone morphogenetic protein-inducible enhancer. J. Biol. Chem. 273, 21145–21152.17. ... 491]. Nature 394, 909–913.28. Roberts, A.B. & Sporn, M.B. (1990) The transforming growth factor-bs. In Peptide Growth Factors and Their Receptors I (Sporn,M.B. & Roberts, A.B., eds),...
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transforming growth factor-beta protocols
... 142: Transforming Growth Factor-` ProtocolsEdited by: P. H. Howe â Humana Press Inc., Totowa, NJDetection of TGF-` in Body Fluids and TissuesAndrew H. LimperIntroduction Transforming growth ... to platelet type ` transforming growth factor. Science 226, 705–707.41. Kojima, S. and Rifkin, D. B. (1993) Mechanism of retinoid-induced activation oflatent transforming growth factor-` in ... (1997) Transforming growth factor-` signal-ing in epithelial cells. Pharmacol. Ther. 75, 21.16. Jin, G. and Howe, P. H. (1997) Regulation of clusterin gene expression by trans-forming growth...
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báo cáo hóa học:" Enhanced serum concentrations of transforming growth factor-beta1 in simple fatty liver: is it really benign?" pot
... diagnosis.Measurements: Serum concentrations of transforming growth factor-beta1 and ferritin.Results: High concentrations of transforming growth factor-beta1 were noticed in patients suffering ... and collagen deposition. Transforming growth factor-beta1 released by hepatic stellate cells during chronic liver injury plays a critical role in liverapoptosis and fibrogenesis.Objective: To ... apoptosis through thegeneration of reactive oxygen species. Hepatology 2008,47:1983-93.20. Hasegawa T, Yoneda M, Nakamura K, Makino I, Terano A: Plasma transforming growth factor-beta1 level...
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Báo cáo hóa học: " Epidermal growth factor receptor gene copy number in 101 advanced colorectal cancer patients treated with chemotherapy plus cetuximab" doc
... were generated via a computer withQuips genetic workstations and imaging software (Vysis).EGFR gene was visualized as a red signal and the CEP 7was visualized as a green signal. EGFR gene ... DataPatterns FISH Patients %Increased EGFR gene copy number in >50% of cells56/101 56EGFR gene copy number<40% of cells28/101 28EGFR gene amplification 4 4Not evaluable 13 12 Campanella ... the epidermal growth factor receptor by immunohistochemestry. J Clin Oncol 2005, 23(9):1803-1811.18. Moroni M, veronese S, Benvenuti S, et al.: Gene copy number for epidermal growth factor...
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Báo cáo hóa học: " The emerging role of insulin-like growth factor 1 receptor (IGF1r) in gastrointestinal stromal tumors (GISTs)" pdf
... Insulin-like growth factor-I activates gene transcription programs strongly associated with poor breast cancerprognosis. J Clin Oncol 2008, 26:4078-85.33. Donovan EA, Kummar S:Role of insulin-like growth ... tumor growth by promoting invasion and survival. Oncogene2010, 29:251-62.5. Kappel CC, Velez-Yanguas MC, Hirschfeld S, Helman LJ: Humanosteosarcoma cell lines are dependent on insulin-like growth ... Houghton PJ, Daughaday WH, Helman LJ:Insulin-like growth factor II acts as an autocrine growth and motilityfactor in human rhabdomyosarcoma tumors. Cell Growth Differ 1990,1:325-31.18. Zhan S, Shapiro...
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báo cáo hóa học: " Effect of anti-inflammatory agents on transforming growth factor beta over-expressing mouse brains: a model revised" potx
... mice overexpressing transforming growth factor-beta 1. Am J Pathol 1995, 147:53-67.4. Wyss-Coray T, Lin C, Sanan DA, Mucke L, Masliah E: Chronic over-production of transforming growth factor-beta1 ... patho-genesis and effects on the ageing and Alzheimer brain. NeurolRes 2003, 25:611-616.44. Wyss-Coray T, Lin C, Sanan DA, Mucke L, Masliah E: Chronic over-production of transforming growth ... http://www.jneuroinflammation.com/content/1/1/11Page 2 of 17(page number not for citation purposes)Background Transforming growth factor-β1 (TGF-β1) is a multifunc-tional cytokine implicated in developmental processes,immune...
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Nghiên cứu tạo dòng, biểu hiện và thu nhận hIGF 1 (human insulin like growth factor 1) từ escherichia coli
... Plasmid phIGF-1 Plasmid pET22b PCR thu nhận gene higf-1 Cắt gene bằng cặp enzyme NdeI và BamHI Cắt plasmid pET22b bằng cặp enzyme NdeI và BamHI Nối gene mục tiêu vào plamid bằng phản ứng nối ... gene mã hóa cho T7 RNA polymerase là enzyme phiên mã của T7 promoter. Chủng này được sử dụng cho các hệ thống vetor biểu hiện gene dưới sự kiểm soát của T7 promoter [5]. Dựa vào đặc điểm gene ... bằng cặp mồi T7pro/ T7ter để kiểm tra vị trí chèn của gene higf-1 trên plasmid và bằng cặp mồi 5’-NdeI-higf1/ T7ter để kiểm tra chiều của gene mục tiêu. Thành phần phản ứng: MiliQ 14l ...
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