transforming growth factorβ1 gene

Ngày tải lên: 09/08/2014, 08:22

Ngày tải lên: 11/08/2014, 08:21

Ngày tải lên: 11/08/2014, 08:22

... clinical applications of transforming growth factor-beta (TGF-beta). Growth Factors 1993, 8:1-9. 3. Assoian RK, Komoriya A, Meyers CA, Miller DM, Sporn MB: Transforming growth factor-beta in human ... sequence analysis of simian transforming growth factor-beta cDNA. DNA 1987, 6:239-244. 5. Miller DA, Pelton RW, Derynck R, Moses HL: Transforming growth factor-beta. A family of growth regulatory peptides. Ann ... trans- forming growth factor. Arthritis Research & Therapy Vol 5 No 3 Mi et al. Research article Adverse effects of adenovirus-mediated gene transfer of human transforming growth factor beta...

Ngày tải lên: 09/08/2014, 01:21

Ngày tải lên: 09/08/2014, 10:22

... Weber, P.C. (1996) Effects of dif- ferent polyunsaturated fatty acids o n growth- related early gene expression and cell growth. Lipids 31 (Suppl. ), S37–S40. 7. Sinclair, A., Attar-Bashi, N. & ... reaction (RT-PCR): a sensitive method to examine basic fibroblast growth factor-induced expression of th e early growth response gene- 1 (egr-1) in human umbilical arterial endothelial cells. Mol. ... receptor gamma1 gene expression in human aortic smooth muscle c ells. J. Biol. Chem. 277, 26808–26814. 27. Yan, Y.X., Nakagawa, H., Lee, M.H. & Rustgi, A.K. (1997) Transforming growth factor-alpha...

Ngày tải lên: 19/02/2014, 16:20

Ngày tải lên: 09/08/2014, 10:21

Ngày tải lên: 12/08/2014, 11:22

... constitutively active form of AKT; DM, differentiating medium; GM, growth medium; IGF-1, insulin-like growth factor 1; IGFBP5, insulin-like growth factor 1-binding protein; IRS-1, insulin receptor substrate ... role primarily during embryogenesis and regeneration [28,29], IGF-1 has been reported to be essential for muscle differentiation and hypertrophy [1–3]. Unlike other growth factors, IGF-1 is able ... C2 myoblasts overexpressing the insulin-like growth factor 1 binding protein 5 and therefore not responding to endogenously produced insulin-like growth factor 1. Although Neu2-transfected myoblasts...

Ngày tải lên: 19/02/2014, 06:20

... signaling during transforming growth factor- beta-induced smooth muscle differentiation. J Biol Chem 281, 1765–1770. 143 Deaton RA, Su C, Valencia TG & Grant SR (2005) Transforming growth factor-beta1-induced ... localization of transforming growth factor- beta in idiopathic pulmonary fibrosis. Am J Respir Cell Mol Biol 5, 155–162. 153 Broekelmann TJ, Limper AH, Colby TV & McDonald JA (1991) Transforming growth ... essential for transforming growth factor-beta- mediated stress fiber formation. J Biol Chem 276, 15362–15368. 159 Engel ME, Datta PK & Moses HL (1998) RhoB is stabilized by transforming growth factor...

Ngày tải lên: 07/03/2014, 01:20

... or TSC2 have higher mTOR activity, result- ing in a robust cell growth and tumorigenesis [9,10,17,18]. Although it is clear that growth factor- induced activation of Akt blocks TSC1 ⁄ TSC2 inhibi- tion ... control cell growth and sur- vival. Cell 115, 577–590. 15 Inoki K, Li Y, Xu T & Guan KL (2003) Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling. Genes Dev 17, ... during normal Drosophila development. Genes Dev 18, 2479–2484. 21 Huang J & Manning BD (2008) The TSC1-TSC2 com- plex: a molecular switchboard controlling cell growth. Biochem J 412, 179–190. 22...

Ngày tải lên: 15/03/2014, 11:20

... Site-directed mutagenesis Plasmid pD7egrGFL was generated by exchange of đve nucleotides in CRE2 (nucleotides )71 to )58) from ACGTC t o CTCAT by site directed mutagenesis u sing a kit (Stratagene). The ... many downstream genes and thus r egulation of their expression. Target genes of the Egr-1 transcription factor are numerous and include growth factors such as platelet derived growth factor A ... hans-peter.rodemann@uni-tuebingen.de Abbreviations: Egr-1, early growth response-1 gene; PDGF, platelet derived growth factor; bFGF, basic đbroblast growth factor; SRE, serum response element; MAPK, mitogen...

Ngày tải lên: 17/03/2014, 17:20

... anti-inflammatory cytokine transforming growth factor-b1 (TGF-b1) of the proinflammatory cytokine TNF-a via the induction of FXR1 is the focus of this article. TGF-b1, a member of the large transforming growth factor-b ... 3Â-UTR contains AU-rich elements (AREs). AREs, which are found in many cytokine, inflammatory gene and oncogene mRNAs, are targets for binding proteins that regulate mRNA stability [14]. Mice with ... cachectin. Science 234, 470–474. 7 Parrillo JE (1993) Pathogenetic mechanisms of septic shock. N Engl J Med 328, 1471–1477. 8 Cohen J (2002) The immunopathogenesis of sepsis. Nature 420, 885–891. 9 Weil...

Ngày tải lên: 22/03/2014, 21:21

... arylhydrocarbon receptor expression by transforming growth factor-b 1 . Mol. Pharmacol. 59, 716 724. 22. Do ă hr, O. & Abel, J. (1997) Transforming growth factor-beta 1 coregulates mRNA expression ... of a Smad binding element (SBE) in the JunB promoter that acts as a transforming growth factor-beta, activin, and bone morphogenetic protein- inducible enhancer. J. Biol. Chem. 273, 21145–21152. 17. ... 491]. Nature 394, 909–913. 28. Roberts, A.B. & Sporn, M.B. (1990) The transforming growth factor-bs. In Peptide Growth Factors and Their Receptors I (Sporn, M.B. & Roberts, A.B., eds),...

Ngày tải lên: 31/03/2014, 23:20

... 142: Transforming Growth Factor- ` Protocols Edited by: P. H. Howe â Humana Press Inc., Totowa, NJ Detection of TGF-` in Body Fluids and Tissues Andrew H. Limper Introduction Transforming growth ... to platelet type ` transforming growth factor. Science 226, 705–707. 41. Kojima, S. and Rifkin, D. B. (1993) Mechanism of retinoid-induced activation of latent transforming growth factor-` in ... (1997) Transforming growth factor-` signal- ing in epithelial cells. Pharmacol. Ther. 75, 21. 16. Jin, G. and Howe, P. H. (1997) Regulation of clusterin gene expression by trans- forming growth...

Ngày tải lên: 11/04/2014, 07:14

... diagnosis. Measurements: Serum concentrations of transforming growth factor-beta1 and ferritin. Results: High concentrations of transforming growth factor-beta1 were noticed in patients suffering ... and collagen deposition. Transforming growth factor-beta1 released by hepatic stellate cells during chronic liver injury plays a critical role in liver apoptosis and fibrogenesis. Objective: To ... apoptosis through the generation of reactive oxygen species. Hepatology 2008, 47:1983-93. 20. Hasegawa T, Yoneda M, Nakamura K, Makino I, Terano A: Plasma transforming growth factor-beta1 level...

Ngày tải lên: 18/06/2014, 15:20

... were generated via a computer with Quips genetic workstations and imaging software (Vysis). EGFR gene was visualized as a red signal and the CEP 7 was visualized as a green signal. EGFR gene ... Data Patterns FISH Patients % Increased EGFR gene copy number in >50% of cells 56/101 56 EGFR gene copy number <40% of cells 28/101 28 EGFR gene amplification 4 4 Not evaluable 13 12 Campanella ... the epidermal growth factor receptor by immunohistochemestry. J Clin Oncol 2005, 23(9):1803-1811. 18. Moroni M, veronese S, Benvenuti S, et al.: Gene copy number for epidermal growth factor...

Ngày tải lên: 18/06/2014, 16:20

... Insulin-like growth factor-I activates gene transcription programs strongly associated with poor breast cancer prognosis. J Clin Oncol 2008, 26:4078-85. 33. Donovan EA, Kummar S: Role of insulin-like growth ... tumor growth by promoting invasion and survival. Oncogene 2010, 29:251-62. 5. Kappel CC, Velez-Yanguas MC, Hirschfeld S, Helman LJ: Human osteosarcoma cell lines are dependent on insulin-like growth ... Houghton PJ, Daughaday WH, Helman LJ: Insulin-like growth factor II acts as an autocrine growth and motility factor in human rhabdomyosarcoma tumors. Cell Growth Differ 1990, 1:325-31. 18. Zhan S, Shapiro...

Ngày tải lên: 18/06/2014, 16:20

... mice overexpressing transforming growth factor-beta 1. Am J Pathol 1995, 147:53-67. 4. Wyss-Coray T, Lin C, Sanan DA, Mucke L, Masliah E: Chronic over- production of transforming growth factor-beta1 ... patho- genesis and effects on the ageing and Alzheimer brain. Neurol Res 2003, 25:611-616. 44. Wyss-Coray T, Lin C, Sanan DA, Mucke L, Masliah E: Chronic over- production of transforming growth ... http://www.jneuroinflammation.com/content/1/1/11 Page 2 of 17 (page number not for citation purposes) Background Transforming growth factor-β1 (TGF-β1) is a multifunc- tional cytokine implicated in developmental processes, immune...

Ngày tải lên: 19/06/2014, 22:20

... Plasmid phIGF-1 Plasmid pET22b PCR thu nhận gene higf-1 Cắt gene bằng cặp enzyme NdeI và BamHI Cắt plasmid pET22b bằng cặp enzyme NdeI và BamHI Nối gene mục tiêu vào plamid bằng phản ứng nối ... gene mã hóa cho T7 RNA polymerase là enzyme phiên mã của T7 promoter. Chủng này được sử dụng cho các hệ thống vetor biểu hiện gene dưới sự kiểm soát của T7 promoter [5]. Dựa vào đặc điểm gene ... bằng cặp mồi T7pro/ T7ter để kiểm tra vị trí chèn của gene higf-1 trên plasmid và bằng cặp mồi 5’-NdeI-higf1/ T7ter để kiểm tra chiều của gene mục tiêu. Thành phần phản ứng: MiliQ 14l ...

Ngày tải lên: 25/07/2014, 08:29