transcription electroporation and generation of infectious bvdv
Báo cáo khoa học: " Molecular characterization and phylogenetic analysis of the complete genome of a porcine sapovirus from Chinese swine" pdf
Ngày tải lên :
12/08/2014, 04:21
... mM) of each primer, 10 μL of template and adding sterilize H2O to 50 μL The reaction was done with the following profile: Activation of DNA polymerase at 95°C for min, followed by 35 cycles of ... project XH was the leader of the project SY and WZ amplified the complete genome and analyzed the genome profile QS and FH went on the sequence alignment All authors read and approved the final ... large polyprotein; and ORF2 encoding a small basic protein (VP2) of unknown function B Schematic of the conserved nucleotide sequence motifs at the 5' termini of the genomic and predicted subgenomic...
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Báo cáo khoa học: Modified PCR methods for 3¢ end amplification from serial analysis of gene expression (SAGE) tags doc
Ngày tải lên :
07/03/2014, 00:20
... II: 22 lL of H2O, 15 lL of m NaClO4, and 38 lL of 2-propanol Precipitation mixture III: 64 lL of a 100% ethanol ⁄ m NaAc mixture (25 : 1), lL of glycogen (20 mgÆmL)1; Roche), and 10 lL of H2O Master ... polymerase (5 UÆlL)1), and ddH2O up to a total volume of 28.6 lL Precipitation mixture I: lL of glycogen (20 mgÆmL)1; Roche, Indianapolis, IN), 15 lL of 7.5 m NH4Ac, and 84 lL of 100% ethanol Precipitation ... (Ptag) and UP-II Step PCR product isolation and sequencing After visualizing specific PCR product on an agarose gel, excise and purify individual bands, and cloned into T-vectors Step Verification of...
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Báo cáo hóa học: " Comparative analysis of full genomic sequences among different genotypes of dengue virus type 3" pdf
Ngày tải lên :
20/06/2014, 01:20
... dataset consisting of a total of 168 sequences and the prM and partial E gene (705 nt) dataset of a total of 195 sequences were used for phylogenetic analysis A complete list of the sequences along ... to be of the same length and sequence as the prototype strain H87 in this study DENV-3 Viral Sequence and Phylogenetic analysis A total of 25 complete genomic sequences of DENV-3 strains and one ... the Thailand isolates into genotype II Genotype III of DENV-3 consists of the strains from Sri Lanka, India, Africa and Samoa that were recently introduced into Central and South America and caused...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
Ngày tải lên :
20/02/2014, 01:20
... deletion mutants of the 3¢ terminus of the minus-strand RNA but deletions were made on the basis of the structure of the 5¢UTR of the plus-RNA, the structure of the 3¢-end of the minusstrand RNA being ... 3873 Binding and replication of 3¢-end of HCV minus RNA T Astier-Gin et al Fig Secondary structure of the 3¢ terminal sequences of HCV minus-strand RNA Model structures of domain I (A) and domain ... Binding and replication of 3¢-end of HCV minus RNA Fig Secondary structure of RNA mutated in the SL-A1 and SL-B1 domains Only the secondary structure of the 151 nt from the 3¢-end of the minus-strand...
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Báo cáo khoa học: "Phrasal Analysis of Long Noun Sequences" pptx
Ngày tải lên :
08/03/2014, 18:20
... must keep in mind that the choice of domain greatly influences the areas of emphasis and interest in language analysis N G P is capable of handling several forms of noun groups that we have not ... understanding The knowledge PHRAN has of the language is stored in the form of pattern-concept pairs (PCPs) The linguistic component of a pattern-concept pair is called a phrasal pattern and describes ... involved in the recognition of long noun sequences, and in Section we present our proposed solution and describe its implementation Sections and are devoted to related work and to our conclusions,...
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Báo cáo khoa học: Molecular analysis of the interaction between cardosin A and phospholipase Da Identification of RGD/KGE sequences as binding motifs for C2 domains pdf
Ngày tải lên :
30/03/2014, 11:20
... terms of charge and is positioned at the tip of a loop and is therefore reminiscent of RGD sequences present in integrin-binding molecules because of its exposed location On the basis of these structural ... superimposition of the 3D structures of kistrin [32] and cardosin A (Fig 8) The importance of both motifs and in particular their basic residues was further emphasized by the complete lack of interaction ... examination of the X-ray structure of cardosin A (PDB code 1B5F) revealed also a KGE motif at the tip of a loop protruding away from the core of the protein This amino-acid motif mimics RGD in terms of...
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Báo cáo hóa học: " Research Article Convergence Theorems for Partial Sums of Arbitrary Stochastic Sequences" pdf
Ngày tải lên :
21/06/2014, 07:20
... sequences of independent random variables are the particular cases of this paper In addition, the main theorems of this paper extend the main results by Wang and Guo in 2009, Qiu and Yang in 1999, and ... case of Theorem 2.1 when the random |x|p variables Xn are replaced by Xn /an and φn x Theorem 2.3 Let {Xn , n ≥ 1} be a sequence of arbitrary random variables Let Fn and F0 {Ω, Φ}, n ≥ Let φn and ... A, Journal of Inequalities and Applications Proof It is a special case of Theorem 2.8 when ξn |x|p /np/2−1 log n p−2 log n, cn n, φn x |x|p , and ϕn x |x|p here, we set ϕ1 x Proofs of Theorems...
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Báo cáo hóa học: " Research Article Mean-Square Performance Analysis of the Family of Selective Partial Update NLMS and Affine Projection Adaptive Filter Algorithms in Nonstationary Environment" doc
Ngày tải lên :
21/06/2014, 08:20
... (n)} and independent of the x(k) for all k and of the d(k) for k < n T (·) : transpose of a vector or a matrix, Derivation of SPU Adaptive Filter Algorithms Tr(·): trace of a matrix, Different ... vector of filter coefficients is partitioned into K blocks each of length L and in each iteration a subset of these blocks is updated For this family of adaptive filters, the matrices C(n) and W(n) ... the and blocks each of length L on the diagonal and the positions of 1’s on the diagonal determine which coefficients should be updated in each iteration The positions of blocks (N blocks and N...
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Period 9 UNIT 2: CLOTHING Lesson 3: Listening I. Aims of the lesson : - By the end of the lesson, potx
Ngày tải lên :
03/07/2014, 19:20
... Pre - listening *New words: - Listen and guess - announcement: the the meanings New words: (translation): Thông báo of the words from - - missing: (synonym of lost): Thất T's eliciting announcement: ... lạc - an entrance: (synonymy of a door) - fair: (n) Hội chợ Lối vào - an entrance: Lối vào - a doll: (realia) búp bê Go to the board - a doll: (n) búp bê Cheek: R O R and rewrite the vocabs * Pre- ... questions - Ask students to look at the pictures - Look at the Guessing on page 16 and answer the following pictures and questions answer the ? What are these? questions ? What is this? These are...
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Giáo án lets go 1 - UNIT 3: LET’S LEARN Week: 21 Period: 42 I. Objectives: By the end of the ppt
Ngày tải lên :
23/07/2014, 15:20
... objects,chararct ers - Listen and show on the characters - Listen and choral repetition Asking groups a crayon, crayons; a pencil case, pencil cases; a table, tables; ask and a cassette, cassettes; ... Jenny: Scott: 22: pageIt’s a pencil case Andy: What are these? They’re cassettes Kate: Devidin g class into 02 groups Showin g teacher’ s cards answer - Listen and choral repetition - Group A: What ... notebooks.answer Vocabulary Practice: What’s this? It’s a crayon - Listen and show on the objects, page 23 - Listen and choral Hanging repetition the wall chart What are these? They’re caryons...
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English Language Proficiency Tests-Advanced Level''''sarchiveAt the End of the Day (3) pptx
Ngày tải lên :
25/07/2014, 07:20
... think it's time for me to hit the sack, which need be no of the softness of the mattress criterion critique criticism critical 3 .And very important people are not described as simply going ... dignified dignify dignitary 5 .Of course should your guests be to go home to their own beds uncertain unclear ungrateful unwilling 6 .and you have a strong desire to be of them and go to sleep riddance ... the best thing is to drop off or nod off in the middle of the conversation restrictions inhibitions prohibitions cprohibitions 10 .And if all else go upstairs and come down again in your pyjamas...
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English Language Proficiency Tests-Advanced Level''''sarchiveAt the End of the Day (3) docx
Ngày tải lên :
25/07/2014, 11:21
... think it's time for me to hit the sack, which need be no of the softness of the mattress criterion critique criticism critical 3 .And very important people are not described as simply going ... dignified dignify dignitary 5 .Of course should your guests be to go home to their own beds uncertain unclear ungrateful unwilling 6 .and you have a strong desire to be of them and go to sleep riddance ... the best thing is to drop off or nod off in the middle of the conversation restrictions inhibitions prohibitions cprohibitions 10 .And if all else go upstairs and come down again in your pyjamas...
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Báo cáo y học: "Expression pattern analysis of transcribed HERV sequences is complicated by ex vivo recombination" ppsx
Ngày tải lên :
13/08/2014, 05:22
... HML-2 proviruses http://www.retrovirology.com/content/4/1/39 Generation of recombinants in the course of reverse transcription of RNA and subsequent PCR is a well known phenomenon Retroviral reverse ... other species and other classes of repetitive elements Conclusion RT and PCR experiments will probably be instrumental to examine presence of a recently proposed replicating and infectious HERV-K(HML-2) ... instances of replicating and infectious HML-2 variants Besides, our findings may be taken as a reminder of potential pitfalls when investigating repetitive sequences Methods Generation of HML-2...
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Báo cáo y học: " Analysis of 14 BAC sequences from the Aedes aegypti genome: a benchmark for genome annotation and assembly" docx
Ngày tải lên :
14/08/2014, 07:21
... of over-prediction, genesplitting and incorporation of random and transposon-based ORFs into gene models Masking of repeat sequences before gene prediction reduced the number of gene models and ... regions, and orthologous and synSummary of each BAC clone Additionalpresence of of the assembly and annotation of each BAC Acknowledgements The authors would like to thank R Bruggner and E.O Stinson ... online version of this paper Additional data file contains detailed descriptions of the assembly and annotation of each BAC clone, the presence of replicated regions, and orthologous and syntenic...
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End of the Tether
Ngày tải lên :
06/11/2012, 17:32
... him of this kind of fame The piercing of the Isthmus of Suez, like the breaking of a dam, had let in upon the East a flood of new ships, new men, new methods of trade It had changed the face of ... seas and the very spirit of their life; so that his early experiences meant nothing whatever to the new generation of seamen In those bygone days he had handled many thousands of pounds of his ... for the land, and by -and- by three palms would appear on the sky, tall and slim, and with their disheveled heads in a bunch, as if in confidential criticism of the dark mangroves The Sofala would...
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TCHON AV9 HKII PERIOD 3 ADVERB CLAUSES OF REASON
Ngày tải lên :
20/07/2013, 01:28
... should you to……………… the environment ? ( keep / protect / prevent / reduce) ……………… is one of the cause of flood ( Forest / Forestry / Deforestation / Forests) We …………… up our neighborhood when ... ………………………………………………………… …………………………………… Tom is trying to learn English He wants to travel to England ………………………………………………………… …………………………………… My class was happy We won the football match last Saturday ... conservationist arrived ( cleaned/ cleaning/ are cleaning/ were cleaning) …….are used to kill inserts and weeds so as to help crops grow better.( Pesticides / Dynamite/ Treasures / Creatures) III/ Put...
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DESIGNING AN END OF YEAR ENGLISH OBJECTIVE TEST FOR 1ST YEAR NON MAJOR ENGLISH STUDENTS OF THE ACADEMY OF FINANCE
Ngày tải lên :
07/09/2013, 13:19
... interpreted and evaluated them in terms of frequency distribution, central tendency and dispersion More carefully, each test item was also analyzed and interpreted in terms of level of difficulty and ... there are two components of test reliability: the performance of candidates from occasion to occasion, and the reliability of the scoring Therefore, he suggests several ways of achieving consistent ... numbers of candidates Meanwhile, in a subjective test, candidates must think of what to say and then express their ideas as well as possible Since subjective tests allow for much greater freedom and...
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