time pcr assay using 5 ht2a 5 htt and insr

... RTQ -PCR vs the previous real -time PCR assay To compare the ultra sensitive RTQ -PCR assay with the previously reported test, HBV-DNA derived from 25 randomly selected samples was quantified using ... occasions (50 %), while IU/mL were detected on out of occasions ( 25% ) The 95% and 50 % detection end-point of the assay were 22.2 and 8.4 IU/mL respectively Performance of ultra sensitivity RTQ -PCR assay ... sensitive RTQ -PCR and the previous test was: log10 HBV-DNA (copies/mL) with the ultra sensitive RTQ -PCR = -0 .53 1 + 1.015x log 10 (copies/mL) with the previous real -time PCR test The 95% CI for the...

Ngày tải lên: 12/08/2014, 04:20

... non-cytotoxic concentrations of ribavirin (62 .5 μg/ml, 1 25 μg/ml, 250 μg/ml, 50 0 μg/ml and 750 μg/ml) The cytotoxicity assay was carried out using MTT assay (Chemicon) Virus yield from the supernatants ... forming assays [12], and serial diluted accordingly to the plaque forming units (PFU) per ml One-step SYBR greenbased RT -PCR was optimized and carried out using the SYBR Green Quantitative RT -PCR ... method as the gold standard for virus detection The real -time PCR assays have many advantages comparing to conventional PCR including simplicity, rapidity, sensitivity, and low Ho et al Virology...

Ngày tải lên: 12/08/2014, 04:21

... temperature was 52 °C Fluorescent quantitative real -time PCR standard curve establishment The FQ -PCR amplification curves and the corresponding fluorescent quantitative real -time PCR standard curve ... TaqMan real -time PCR method Results Development and optimization of FQ -PCR and conventional PCR Following the optimization of FQ -PCR, final concentrations of primers each of 0.3 μmol/L and probe ... 0.3, 0.4 and 0 .5 μmol/L), probe con- http://www.virologyj.com/content/6/1/71 centrations (0. 05, 0.1, 0.2, and 0.3 μmol/L) and MgCl2 concentrations (2, 4, and mmol/L) The conventional PCR was performed...

Ngày tải lên: 12/08/2014, 04:21

... temperature was 52 °C Fluorescent quantitative real -time PCR standard curve establishment The FQ -PCR amplification curves and the corresponding fluorescent quantitative real -time PCR standard curve ... TaqMan real -time PCR method Results Development and optimization of FQ -PCR and conventional PCR Following the optimization of FQ -PCR, final concentrations of primers each of 0.3 μmol/L and probe ... 0.3, 0.4 and 0 .5 μmol/L), probe con- http://www.virologyj.com/content/6/1/71 centrations (0. 05, 0.1, 0.2, and 0.3 μmol/L) and MgCl2 concentrations (2, 4, and mmol/L) The conventional PCR was performed...

Ngày tải lên: 12/08/2014, 04:21

... đoạn quang 95oC phút 95oC 10 phút Giai đoạn - Bƣớc 95oC 25 giây 95oC 15 giây - Bƣớc 62oC 30 giây 60oC phút - Bƣớc 72oC 45 giây Số chu kỳ 50 Giai đoạn 72oC x 45 4oC phút 4oC 49 x 50 Cài đặt phần ... xuôi 0 ,5 µM - Mồi xuôi 0 ,5 µM - Mồi ngược 0 ,5 µM - Mồi ngược 0 ,5 µM - DNA mẫu 10 - 50 ng - Taq polymerase 1,0 U 5U/µl - DNA mẫu 10 - 50 ng - Nước cất siêu Tổng thể tích - Nước cất siêu 25 μl 25 μl ... (GeneScan) - Mồi xuôi - Mồi ngược 0 ,5 µM - DNA mẫu 0 ,5 µM - DNA mẫu - Nước cất siêu 50 ng - Nước cất siêu Tổng thể tích 25 μl Tổng thể tích 50 ng 48 25 μl 49 Đặc điểm 35S master mix Các master mix dùng...

Ngày tải lên: 28/07/2014, 04:21

... mẫu sản phẩm real -time PCR cho kết real -time PCR [+] làm tinh để thu nhận sản phẩm PCR với thử nghiệm tinh sản phẩm PCR (PCR clean-up kit) Promega sản xuất (Cat #A9281) Sản phẩm PCR sau tinh điện ... 4492 25. 38 409,173 Unkn 4483 30. 75 13,027 4488 N/A Unkn 4482 N/A Unkn 4487 31.82 6 ,55 4 Unkn 4490 27.61 Unkn 4486 30.2 18 ,54 2 Unkn 4480 N/A Unkn 44 85 N/A 4494 30.79 Unkn 4484 26. 95 149, 353 Unkn ... phương pháp RT TQ real -time PCR mà công ty Nam Khoa phát triển sử dụng rộng rãi nhiều phòng thí nghiệm lâm sàng có trang bị real -time PCR Trong phương pháp RT TQ real -time PCR này, nồng độ HCV...

Ngày tải lên: 01/08/2014, 03:21

... 5- FU + oxa 93 ( 75- 111) 7 .5 Gy 54 (29-80) × 2 .5 Gy 39 (20 -58 ) × 2 .5 Gy + 5- FU 95 (81-110) 7 .5 Gy + oxa 23 ( 15- 31) × 2 .5 Gy + oxa 91 (84-99) 7 .5 Gy + 5- FU + oxa 29 (0 -57 ) 0.007 Conclusion In conclusion, ... × 2 .5 Gy 114 (111-116) Gy + 5- FU 80 (66- 95) × 2 .5 Gy + 5- FU 102 (94-111) Gy + oxa 101 (94-108) × 2 .5 Gy + oxa < 0.0001 102 (91-112) Gy + 5- FU + oxa 88 (77-98) × 2 .5 Gy + 5- FU + oxa 93 ( 75- 111) ... chemotherapy dose A 5- Fluorouracil (5FU), B Oxaliplatin Each data point stands for the mean of each group and error bars represent +/- SD [17], compared to Gy and higher in the present study Using only...

Ngày tải lên: 09/08/2014, 10:20

...    lˆ0 LÀ1 ˆ je…l†jjeÀj!l j lˆ0 LÀ1 ˆ je…l†j: 5: 5:6† lˆ0 As shown in (3 .5. 3), D ˆ 2ÀB : je…l†j 5: 5:7† L Á 2ÀB : 5: 5:8† Thus Equation (5. 5.6) becomes jE…!†j This bound is too conservative ... EXPERIMENTS USING THE TMS320C55X Table 5. 1 } } 227 (continued ) n‡‡; x [ ˆ (int)((0x7fff*temp)‡0 .5) i] ; 5. 6.1 Experiment 5A ± Implementation of Block FIR Filter The difference equation (5. 2.1) and Example ... (a) (b) Figure 5. 25 Input and output signals of Experiment 5A: (a) input signals in the frequency (top) and time (bottom) domains, and (b) output signals in the frequency (top) and time (bottom)...

Ngày tải lên: 23/10/2013, 22:15

... 5: Creating and Manipulating Trees Using DOM Lab 5: Changing a DOM Tree ! Exercise 1: Using DOM with Inline Data ! Exercise 2: Using DOM with an External File ! Exercise 3: Manipulating DOM and ... completion Module 5: Creating and Manipulating Trees Using DOM 19 ! Run your DOM application from the command line Click Start, and then click Run Type cmd, and then click OK to open a command prompt ... 6.0 and is effectively a new language For more information about Visual Basic NET, see Course 255 9A, Introduction to Visual Basic NET Programming Module 5: Creating and Manipulating Trees Using...

Ngày tải lên: 10/12/2013, 16:15

... available), or black 3 .5 dB/km at 850 nm 1 .5 dB/km at 1300 nm 50 0 MHz.km at 850 nm (OFL) 50 0 MHz.km at 1300 nm 0.2 55 0 m at 850 & 1300 nm 86 m at 850 nm OM3 Specification 50 /1 25 μm Fibre a) b) Sheath ... Requirements 15 5.2.2 Reference Setting (Zeroing) Methods for LSPM Testers on MMF and SMF 15 Class D Tender Spec ADC KRONE 06 050 8 Page 5. 2.3 5. 2.4 5. 2 .5 5.2.6 Use of Mandrels or Coils (for ... Warranty

Ngày tải lên: 21/12/2013, 13:15

... out of hand! After two minutes they open their eyes and describe and discuss what they heard, first with their neighbor and then with the class as a whole Note: Both the simple past tense and continuous ... banking, tourism, technology, diplomacy and scientific research And as Crystal, 1997 said: “ today, we are concerned with global ecology and global economy And English has been described as a global ... prerequisite and difficult condition for all teachers They toss in their sleep about how to teach children well and get them really actively involved in all the classtime The present time left no...

Ngày tải lên: 27/12/2013, 20:26

... interfaces = 192.168.220.100/ 255 . 255 . 255 .0 \ 134.213.233.110/ 255 . 255 . 255 .0 # Debug logging information log level = log file = /var/log/samba.log.%m max log size = 50 debug timestamp = yes [data] path ... explicitly using NET USE or by mapping a drive letter to it using Windows Explorer It simply prevents information in the browse list from being retrieved by any client that requests it 5. 1 .5. 5 auto ... Workstation 3 .51 16 Windows 98 Windows 95 Windows 3.1 for Workgroups Following that, each computer on the network is assigned a separate value according to its role, as shown in Table 5. 2 Table 5. 2: Computer...

Ngày tải lên: 26/01/2014, 08:20

... = 755 force user = joe force group = accounting delete readonly = yes 5. 3.2 File and Directory Permission Options The options for file and directory permissions are summarized in Table 5. 5; each ... each option is then described in detail Table 5. 5: File and Directory Permission Options Option Parameters Function Default Scope Table 5. 5: File and Directory Permission Options Option Parameters ... most 755 : [data] path = /home/samba/data browseable = yes guest ok = yes writeable = yes directory mask = 755 Alternatively, you can also force various bits with the force create mode and force...

Ngày tải lên: 26/01/2014, 08:20

... automatically detect kernel oplocks and use them if present At the time of this writing, this feature is supported only by SGI Irix 6 .5. 2f and later However, Linux and FreeBSD support are expected ... upper-case characters, followed by a dot, and three more uppercase characters This was known as the 8.3 format, and was a huge nuisance Windows 95/ 98, Windows NT, and Unix have since relaxed this problem ... cause collisions This is called name mangling, and Samba deals with this in a manner that is similar, but not identical to, Windows 95 and its successors 5. 4.1 The Samba Mangling Operation Here is...

Ngày tải lên: 26/01/2014, 08:20

... for each gene and used as the standard sample in the subsequent assay Each standard curve consisted of six serial dilutions (1, 1 /5, 1/ 25, 1/1 25, 1/6 25, and 1/31 25) of the selected standard cDNA ... AMY2A -1.22 0.87 3.68 ** 0.68 2 .50 * 1.90 2.91 * 1.36 BEX1 3. 25 5.69 7.36 ** 3.08 -0.17 2.86 4.72 * 5. 72 CDH1 1. 85 3.70 ** 1.87 3. 85 ** -0.41 5. 20 0.48 -0.70 CSTA 2 .54 ** 4.79 ** -1.06 0.80 0.09 ... of 19 candidate genes by qPCR Using qPCR, we quantified the expression of the 19 candidate genes in the same eight test cell lines (A549, ABC-1, EBC-1, LK-2, LU 65, LU99, STC 1, RERF-LCMA) and the...

Ngày tải lên: 15/02/2014, 04:20

... activity from series of Ad.CA-AMPK concentrations (5, 10, 25 and 50 moiÆcell)1) Some cells were double infected with Ad.MCD (5 moi) and Ad.CA-AMPK ( 25 moi) to study the effect of overexpressed AMPK ... subsequently collected and stored at )80 °C For AMPK and ACC assays, cells were lysed in buffer containing 50 mM Tris-base, 250 mM mannitol, mM EDTA, mM EGTA, 50 mM NaF, 5. 0 mM NaPPi, mM dithiothreitol, ... (Potter-Elvehjem, between 0.10 and 0. 15 mm clearance), and homogenized by 10– 15 up and down strokes while revolving at 50 0 r.p.m Homogenate was then transferred to a microfuge tube, and sucrose was added...

Ngày tải lên: 16/03/2014, 16:20

... chain reaction (PCR) and quantitative real -time PCR have been recently developed and employed for the detection and quantitation of JCV [ 151 9] In clinical specimens, real time- PCR has proven to ... 95 C, followed by 40 cycles of 95 C for 10 sec and 60°C for 15 sec and the amplification fluorescence was read at 60°C at the end of the cycle Real -time PCR amplification data were analyzed using ... 106X - 350 714 and Y = × 106X + × 107 and the r2 were 1.0 and 0. 95 for JCV(Mad1) virus stocks I and II, respectively Page of (page number not for citation purposes) Virology Journal 2006, 3:3 and...

Ngày tải lên: 19/06/2014, 08:20

... chain reaction (PCR) and quantitative real -time PCR have been recently developed and employed for the detection and quantitation of JCV [ 151 9] In clinical specimens, real time- PCR has proven to ... 95 C, followed by 40 cycles of 95 C for 10 sec and 60°C for 15 sec and the amplification fluorescence was read at 60°C at the end of the cycle Real -time PCR amplification data were analyzed using ... 106X - 350 714 and Y = × 106X + × 107 and the r2 were 1.0 and 0. 95 for JCV(Mad1) virus stocks I and II, respectively Page of (page number not for citation purposes) Virology Journal 2006, 3:3 and...

Ngày tải lên: 20/06/2014, 04:20

... solution of singular systems with delay, Control Theory and Application, 15( 4), 54 2 -55 0 Mao, X., (1997) Comments on ”Improved Razumikhin-Type Theorem and its Applications”, IEEE Trans Automat Control, ... Debeljkovic, Lj D., A Jacic, M Medenica, (20 05) Time Delay Systems Stability and Robustness, Faculty of Mechanical Engineering, University of Belgrade, ISBN 86-7083 -50 4 -5 Debeljkovic, D Lj., M P Lazarevic, ... results on singular time delayed system stabilty, Proc ACC 05, June, 8-10 20 05, Portland (Oregon) , CD-Rom Debeljkovic, Lj D., S B Stojanovic, M B Jovanovic, S A Milinkovic, (20 05. c) Further results...

Ngày tải lên: 20/06/2014, 07:20

... dCTP, 1. 25 U Taq DNA polymerase, and 4 .5 mM MgCl2 Real -time PCR reactions were run as follows: 95 C for 10 and 45 cycles of 95 C for 15 s and 60°C for 40 s For a standard curve, serial dilutions ... 17.69 0.7498 4.2 1 05 24.49 0. 258 9 1. 05 24.03 0 .55 97 2.3 103 30.98 0.2092 0 .59 30.22 0 .56 32 1.9 SD, standard deviation; CV, Coefficient of variation using a TaqMan real -time PCR that detected a ... conventional PCR and real -time PCR for PCV2 positive samples Conventional PCR Real time PCR Subtotal + - + 3 - 0 Total +, positive; -, negative Table Comparison between conventional PCR and real -time PCR...

Ngày tải lên: 11/08/2014, 21:21