the various parts of earth biosphere are connected and

Báo cáo khoa học: The signature amidase from Sulfolobus solfataricus belongs to the CX3C subgroup of enzymes cleaving both amides and nitriles Ser195 and Cys145 are predicted to be the active site nucleophiles pot

Báo cáo khoa học: The signature amidase from Sulfolobus solfataricus belongs to the CX3C subgroup of enzymes cleaving both amides and nitriles Ser195 and Cys145 are predicted to be the active site nucleophiles pot

... shows that the members of this second putative triad are in the correct relative orientation. In our model, the Oc atom of Ser171, the amino group of Lys96 and the thiol group of Cys145 are sufficiently close ... bonds of differ- ent substrates [1]. These proteins all share the typical a ⁄ b hydrolase fold and can be grouped on the basis of their catalytic site and preferred substrate [2,3]. The enzymes of ... mutagenesis and their activity assayed with the colorimetric method of Berthelot, appropriately modified for the assay con- ditions of the archaeal enzyme. This assay is based on the measurement of the...

Ngày tải lên: 07/03/2014, 21:20

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Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf

Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf

... calculated from the standard errors associated with the means of the measured values (i.e. by calculating the square root of the sum of the associated individual errors squared). The maximum percentage ... aB-crystallin (i.e. the effect of the additive on the aggregation of the target protein is inde- pendent and different from that of the chaperone). The standard errors associated with this theoretical ... further enhanced by it (Fig. 1C). The sig- nificant decrease in the amount of precipitation in the presence of high concentrations of Arg-HCl in the absence of the chaperone precluded analysis of the...

Ngày tải lên: 18/02/2014, 16:20

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The Health Benefits of Physical Activity for Girls and Women ppt

The Health Benefits of Physical Activity for Girls and Women ppt

... over the age of 65 are less active now than at the end of the eighties. Older women are the least active of all age and sex groups. In fact, middle-aged men and women, along with men over 65, are ... The exercise of daily life: Towards an understanding of the meaning and practice of physical activity in the daily lives of low-income single mothers. Masters Thesis. University of British Columbia: ... of the programs and policies related to physical activity and the health of girls and women are generally outside of the formal health care system. It is our intention to consider diversity, the...

Ngày tải lên: 05/03/2014, 13:20

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Báo cáo khoa học: Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx

Báo cáo khoa học: Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx

... ([6] and references therein), suggesting the presence of detect- able amounts of PMLA synthetase-specific mRNA. The absence of PMLA and polymalatase in other cell types could be the result of cell ... substitutions, eight of them located in the first two b-strands of the N-terminal half of NKA48, upstream of the homodimer interface and accessible for interactions with other macromolecules. It ... affiliated with PMLA metabolism and ultimately to identify the syn- thetase gene, SSH was used with mRNA of the plas- modium as the tester and mRNA of amoebae as the driver. The amoebae strain chosen...

Ngày tải lên: 07/03/2014, 12:20

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Báo cáo khoa học: The oxidative effect of bacterial lipopolysaccharide on native and cross-linked human hemoglobin as a function of the structure of the lipopolysaccharide A comparison of the effects of smooth and rough lipopolysaccharide ppt

Báo cáo khoa học: The oxidative effect of bacterial lipopolysaccharide on native and cross-linked human hemoglobin as a function of the structure of the lipopolysaccharide A comparison of the effects of smooth and rough lipopolysaccharide ppt

... comparison of the effects of rough andsmoothLPSsofE. coli, both in the presence and absence of EDTA (Fig. 1). The rate of oxidation was increased in the presence of the LPSs of both the smooth and rough ... measure of the rate of oxidation of oxyHb. RESULTS The effect of pH on the auto-oxidation of cross-linked Hb was studied. The rate of decrease of the concentration of oxyHb increased as the pH ... effective binding of Hb. The effects of the various LPSs on the oxidation rate of cross-linked Hb can be compared in Figs 1 and 2. The relative rates both in the presence and absence of EDTA are: smooth...

Ngày tải lên: 08/03/2014, 10:20

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A review on the cd content of rice daily cd intake and accumulation in the kidneys

A review on the cd content of rice daily cd intake and accumulation in the kidneys

... populations. Foods and beverages are sampled and bought in representative retail shops in the area and analyzed for the element. The cadmium content of the same food item is averaged and multiplied ... content in soil and rice is low within the same area but high between different areas 26) . Daily cadmium intake There are four ways of estimating of daily cadmium intake. The first is another meal ... zinc, and copper levels in the kidneys and liver of the inhabitants of north-eastern Poland. Pol J Occup Med Environ Health 1993; 6: 133–141. 41) / Orlowski C, Piotrowski JK, Kubów M. The levels of cadmium,...

Ngày tải lên: 15/03/2014, 23:11

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Báo cáo khoa học: The use of synthetic linear tetrapyrroles to probe the verdin sites of human biliverdin-IXa reductase and human biliverdin-IXb reductase potx

Báo cáo khoa học: The use of synthetic linear tetrapyrroles to probe the verdin sites of human biliverdin-IXa reductase and human biliverdin-IXb reductase potx

... reduce the three other biliverdin isomers IXb,IXd and IXc. Bili- verdin-IXb reductase (BVR-B) catalyses the reduction of only the IXb,IXd and IXc isomers of biliverdin. Therefore, the activity of ... suggest that they may inter- act with the ‘roof’ and ‘floor’ of a hypothetical ‘verdin binding site’, with the nicotinamide ring forming part of the floor. If the bound conformation is helical, there would ... (B) The BVR-B reaction was initiated by the addition of hBVR-B (18.5 lg) and the spectra recorded over a period of 20 min. Fig. 2. The structures of the open chain and locked tetrapyrroles. The...

Ngày tải lên: 16/03/2014, 02:20

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Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy pot

Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy pot

... study the secondary structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also to study the effect of thermal unfolding in the presence and the absence of MgATP. ... esti- mation of the bandwidth and the intensity of the bands making up the amide I or IÂ region. Thereafter, curve tting was performed and the heights, widths and positions of each band were optimized ... When the cat-f spectra obtained in the absence (Fig. 4A) and in the presence (Fig. 4B) of MgATP were compared the widening of the overall amide IÂ contour was less in the presence of MgATP and the...

Ngày tải lên: 16/03/2014, 13:20

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Báo cáo khoa học: Principles behind the multifarious control of signal transduction ERK phosphorylation and kinase/phosphatase control potx

Báo cáo khoa học: Principles behind the multifarious control of signal transduction ERK phosphorylation and kinase/phosphatase control potx

... amplitude and duration of signaling are controlled by the kinases or phosphatases in the system, and whether they are controlled differ- entially by some or all of them. On the basis of the antagonistic ... how the second kinase in the cascade determines the time dependence of the activity of the third kinase, we varied the V max of the second kinase reaction and recalculated the concentration of the ... such as the MAPK cascades, are often composed of kinase and phosphatase pairs. But how important are the kinases and phosphatases relative to each other? Are all combinations of the control of signal...

Ngày tải lên: 16/03/2014, 18:20

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