the unique extraction method for some of vbic mod

... of the VBIC Model 5.5.2 Extraction Methods for the VBIC Model Parameters 5.6 136 138 Parameter Converting Method from the GP Model to the VBIC Model 142 5.6.1 Converting the GP model to the VBIC ... suitable for converting the parameters of one model to those of another one An example is given for the VBIC model extraction by going through the GP model It is based on the fact that the VBIC model ... definition of the model equations and the extraction of the model parameters is named modeling The main objective of modeling is to find the required model parameters for the related model equations...

Ngày tải lên: 17/09/2015, 17:20

... 3B), these data suggest that these domains are not obligate for the stabilization of the dimers, but rather must contribute to the overall stability of the dimers Crystallographic analysis of the ... activity of each of the mutants is given in parentheses It was determined by setting the Vmax adjusted activity of CD38-WT to 100% and then calculating the percentage activity of each of the mutants ... between the various mutants and is reported as nmol of product formed per minute per arbitrary unit of CD38 Briefly, the total amount of protein per Ba/F3 cell was determined by the Bradford method The...

Ngày tải lên: 23/03/2014, 12:20

... showing immunolocalization of asporin in the human disc The objectives of the present study, therefore, were to determine the localization patterns of asporin within the human and sand rat intervertebral ... asporin in the outer annulus of asporin the human disc Note that there are some cells that not show asporin localization (arrow) (b) The presence of asporin is shown in the inner annulus of the human ... age for the six grade III discs was 45.5 years, and 53 years for the five grade IV discs Asporin immunolocalization The first portion of our study of asporin and the intervertebral disc tested for...

Ngày tải lên: 09/08/2014, 14:20

... essential for the formation of synovial lining structures in vitro and for the development of inflammatory arthritis in mice [14,15] The morphological hallmarks of RA include activation of FLSs; ... contributions AKHE did the major design of the study and the coordination and establishment of the biobank, carried out the experiments, performed the imaging and FACS analysis and drafted the manuscript ... participated in the design of the study and performed the Larsen scoring of radiographs TE and CA included the patients in the study and collected patient data and tissue samples CJ performed the SDS-PAGE...

Ngày tải lên: 12/08/2014, 15:22

... difference in the occurrence of the TT genotype in any of the studied groups (P>0.05) Also the prevalence of the different genotypes did not deviate from the Hardy–Weinberg equilibrium for the control ... 192 Figure The effect of the T allele on the plasma homocysteine in the different studied groups Bar chart illustrating the effect of the T allele on the level of homocysteine in the different ... level of fruits and green vegetables in the diet of the Egyptian population in general However, the lack of correlation between the plasma tHcy and the folate could be explained by the presence of...

Ngày tải lên: 03/11/2012, 09:49

... in the dark glands of H perforatum [12] The presence of emodin in only the dark glands in H perforatum suggests that emodin biosynthesis may take place in the glands [12] The restriction of HpPKS2 ... layers The HpPKS2 transcripts were found to be present in the large cells and also in the some of the innermost flat cells of the nodules In the flower petals of H perforatum, two types of multicellular ... both the large cells and some of the innermost flat cells of the multicellular nodules This indicates that if HpPKS2 is involved in the biosynthesis of hypericins, then at least the early phase of...

Ngày tải lên: 18/02/2014, 18:20

... transactivating module present in the unique N-terminus, (b) binding of the N-terminus by ancillary factors, (c) the lack of a negatively functioning module that is present in the N-terminus of SAF-1 ... from other transcription start sites, and are minor transcription products Nested RT-PCR analysis was performed for further verification of the existence of novel exon 1A in SAF-3 A product of the ... transcriptionally active than SAF-1 These results shed light on the relevance of the generation of multiple distinct functional SAF isoforms, and imply the existence of Transcription factor SAF-3 is...

Ngày tải lên: 07/03/2014, 02:20

... 45 °C for 24 h Assessment of the conformation of Ser52Arg neuroserpin The most likely cause for the inactivity of Ser52Arg neuroserpin and inaccessibility of the reactive loop is that the mutant ... polymers of wild-type and Ser49Pro neuroserpin More detailed unfolding experiments were then performed to further assess the conformation of wild-type Fig Characterization of the conformation of Ser52Arg ... and the resistance of the reactive loop to cleavage The lower portion of b-sheet A is filled by unfolding of the F-helix (yellow) Right, the F-helix is displaced by the reactive loop of another...

Ngày tải lên: 07/03/2014, 16:20

... similar to the TG of vertebrates and arthropods [34] Its molecular mass is within the 75–90-kDa range known for the TG of these organisms [34] The most unique property of nTG, not found in other TGs, ... strategy for amplification of nTG cDNA The horizontal bar indicates the nTG cDNA Thick horizontal bars indicate the sequences of PCR-amplified clones The primers used for PCR are given on the right The ... (S) or the immunoprecipitates (IP) was measured, and is expressed as the percentage of the total activity in the 10 lL of concentrated Sup4 (Input) The results shown are the averages of three...

Ngày tải lên: 08/03/2014, 10:20

... dependent on the type of catalyst This could be explained by the effect of the catalyst on the barrier energy to the nucleation of a new step at the growth front of the nanowires (i.e., the liquid/solid ... [11] The dependence of the growth rate on the reactant vapour concentration is not in itself evidence that any of them is the rate-determining step Rather, it simply reflects the dependence of the ... supports the argument that Pt enhances the rate of incorporation of Si from the liquid phase to the lattice The present results suggest that Pt is a good candidate for the VLS growth of Si nanowires...

Ngày tải lên: 16/03/2014, 15:05

... could minimize the total energy of the nanowire because the surface energy of the {1 1} facets is the lowest and the energy of the side surfaces dictates the total surface energy of a SiNW [11] ... by the OAG method without any catalysts As shown in Fig 4a, the side surfaces of the SiNW are made of the {1 1} and {1 0} facets for the nanowire grown along [1 2] direction The presence of those ... array of aligned nanowires The average length of the nanowires was less than lm, while the density of the wires varied with the substrate or deposition temperature The largest density ($400/lm2) of...

Ngày tải lên: 16/03/2014, 15:06

... Ishikawa RTPCR was used to confirm the expression of prosemin mRNA in some cell lines A band of the predicted size (500 bp) of the PCR product was observed for most of the cell lines tested, including ... expressed strongly in the pancreas, but weakly in the prostate and cerebellum (Fig 4) The nucleotide sequences of the cDNA and the cosmid clone are identical A comparison of the cDNA and genomic ... alignment of these proteases shows that 10 of the 12 cysteine residues in the prosemin zymogen are conserved among these proteases Cys41 is predicted to form a disulfide bond with Cys175, as in other...

Ngày tải lên: 16/03/2014, 23:20

... more than 50% of the native enzyme (Fig 1C) The distribution of the KE2 mutant between the two fractions was similar to that of the native enzyme (Fig 1C) In contrast, the activity of the KE2RS2 ... and KM20L2 cells (Fig 4B) The expression of other sPLA2s (IB, IIE and IIF) was undetectable The expression of other enzymes involved in the PGE2biosynthetic pathway in these colon carcinoma cell ... IIF, V and X (left) and for cPLA2a (right) After staining of the gel with ethidium bromide, samples were taken for Southern blotting using cDNA probes for the mixture of these sPLA2s RT-PCR (Fig...

Ngày tải lên: 18/03/2014, 01:20

... vivo, ATP turnover rate therefore being an important determinant of the length of time for which a client remains Hsp90-bound [39–41] The question therefore arises of whether more inefficient Hsp90b ... [3,14,18] We used the yeast two-hybrid (Y2H) system to determine the relative strengths of in vivo interaction of these two MAP kinases with the two isoforms of human Hsp90 In the yeast Hsp90s, ... increase the final biomass yields of PP30[pHSP82], relative to the other strains tested (Fig 6) In addition, at high temperature (37 °C as compared to 30 °C), the presence of the Hsp82 isoform of yeast...

Ngày tải lên: 23/03/2014, 07:20

... mature form progressively appeared and accumulated in the supernatants at chase times of 60–120 Because at T0 only the 70 kDa form is present it must be the predecessor of the 80 kDa soluble form The ... immunoprecipitation with the mAb V.1 At T0, the cells contained an immature 70 kDa form of GPV which did not appear to progress to a higher molecular mass form during the 180 of chase On the other hand, an ... the recent and future development of mouse strains mutated in the GPIb–V–IX complex and the availability of efficient culture systems for megakaryocyte precursors [24,25] The requirement for the...

Ngày tải lên: 23/03/2014, 13:20

... in multiple species However, the dn/ds ratio is higher for exon 10 (0.40) than for the other exons (0.13) As the ds value is similar for exon 10 (0.62) and the other exons (0.56), this difference ... In addition to the relatively high value of dn/ds for exon 10, it contains most of the (S/T)PXX repeats found in both mNARG2 (seven of 11) and hNARG2 (four of six) The prevalence of this motif ... for signaling, but rather modulate the transmission of signals through the Wnt/b-catenin pathway by regulating proteins that are critical for signaling We hypothesize that NARG2, similar to the...

Ngày tải lên: 23/03/2014, 13:20

... kb) Reference to the Stanford Radiation Hybrid Map of this region of chromosome 19 gives the most likely order of D19S418/siglec-10/D19S425 with a cytogenetic location of 19q13 The marker D19S418 ... a portion of the transmembrane domain The PCR conditions were: 95 8C for min, followed by 30 cycles of 95 8C/56 8C/72 8C, for 30 s each Table PCR primers used to make probes for Northern blots ... then allowing the red blood cells to settle for 30 The supernatant was harvested and centrifuged for at 500 g The cells were then resuspended in 0.2 Â NaCl/Pi to induce red blood cell lysis for...

Ngày tải lên: 24/03/2014, 04:21

... Materials and methods The results are expressed as the mean ± SEM percentage of G2 for (A) and (B); as the mean ± SEM arbitrary units relative to actin for (C); and as the mean ± SEM for (D) the low ... 20 for h They were then serially incubated with rabbit anti-PHB serum (1 : 650) for h, MitofluorTM Red 589 for 20 min, anti-rabbit FITC (1 : 650) for h and Hoechst 33342 for The cells were then ... 200 lL dimethyl sulfoxide to dissolve the formazan The cells were left for 30 at room temperature The reduction of MTT to formazan was quantified by measuring the absorbance at 540 and 630 nm using...

Ngày tải lên: 29/03/2014, 08:20

... levels for the diagnosis of cancer (EGC) was 67% and 71% (cut-off value of 5.5 ng/ml), and 71% and 67% (cut-off value of ng/ml) The sensitivity and specificity of serum CEA levels for the diagnosis ... (cut-off value of ng/ml) for the detection of cancer (EGC), and 70% and 64% (cut-off value of ng/ml) for the detection of Page of 11 (page number not for citation purposes) Journal of Translational Medicine ... from the greater curvature of the antrum and the midpoint of the greater curvature of the gastric body via endoscopic biopsy In the patients with cancer two specimens were obtained from the cancerous...

Ngày tải lên: 18/06/2014, 15:20

... responsible of some of the sperm collection and viability analysis NG participated in the design of the study, and revised the manuscript FG participated in the design of the study, and revised the manuscript ... in the cytoplasm of some spermatocytes and that of early and late spermatids [13] The flagella of the spermatozoa in the lumen of the seminiferous tubules was also found to be immunopositive for ... protein The FS is a unique cytoskeletal structure surrounding the axoneme and outer dense fibers and defines the extent of the principal region of the sperm flagellum Despite a number of proteins...

Ngày tải lên: 18/06/2014, 15:20