... of Mls1p to peroxisomes is advantageousfor growth on oleic acidTwo steps ofthe glyoxylate cycle take place in the cytosol: the splitting of isocitrate into succinate a nd glyoxylate, and the ... con®rming the speci®city ofthe antibody. Application ofthe an tibody to thin se ctions of wild-type cells grown on oleic acid mediumFig. 1. SKL is required to direct Mls1p tothe peroxisomes ... dilutions of cell cultures (BJ19 91, KM12,KM13) to this medium showed that the mls1D mutant wasunable to form a clear zone (Fig. 4A). On the other hand,despite representing a strictly cytosolic...
... human lamin B recep-tor sequences. Microbiology 14 5, 14 43 14 51. 18 . Simos, G., Maison, C. & G eorgatos, S.D. (19 96) Characterization of p18, a component ofthe lamin B receptor complex and a ... through the RS region of LBR to linker DNA of chromatin s eems to b e predominant.Then, the binding is supported by the interaction ofthe NMregion and a protein(s ) other t han HP1 on sperm ... on the addition of a high concentration of atruncated recombinant LAP2 protein tothe cell-freeXenopus egg extract system [10 ]. Further analysis of the regulation mechanism for the binding of...
... life. Discuss with your partner about this.3. Shopping is a waste of time and money. To what extend do you agree with this statement?4. Discuss the advantages and disadvantages of tourism.5. ... space at the furniture superstore.If you ever decide to visit Britain, you will need to know some ofthe basic rules ofthe sport. First of all, remember that you only need one person to make ... discuss the following topic(Students picked up one among the topics given) 1.The best way to travel is by bicycle. To what extend do you agree with this statement?2. Money isthe most important...
... closed without the decision to initiate any other phases. For example, the project is completed or the risk is deemed too great for the project to be allowed to continue. A Guide tothe Project ... risk management on a project. It consists ofthe Risk Management Planning, Risk Identification, Qualitative Risk Analysis, Quantitative Risk Analysis, Risk Response Planning, and Risk Monitoring ... Management? 8 1. 4 The PMBOK® GUIDE Structure 9 1. 5 Areas of Expertise 12 1. 6 Project Management Context 16 Project Life Cycle and Organization 19 2 .1 The Project Life Cycle 19 2.2...
... 3505–3 511 ª 2005 FEBS 3 511 HYPOTHESISDeath inducer obliterator protein 1 in the context of DNAregulationSequence analyses of distant homologues point to a novel functionalroleAna M. Rojas 1, 2, ... function for the DIDO1 long isoform(Figs 3 and S2). It isthe second domain ofthe elonga-tion factor, TFSII [15 ], that stimulates RNA poly-merase II to transcribe through regions of DNA. The structure ... contains the CXXC signaturebetween the two domains, probably as a result of recombination processes; this signature is missing inDIDO1 and SPP1. The absence ofthe CXXC signa-ture distinguishes...
... apoptosis at 10 0 nM,and high levels of apoptosis were measured at concentra-tions of1 nMand 10 nM. Ala31Leu60-IGF-I failed to prevent serum deprivation-induced apoptosis at 1 nMand 10 ... directlyrelated to ability to b ind tothe IGF1R. Previously we haveexpressed a recombinant f orm o f the human IGF1R byfusing the cDNA encoding the receptor ectodomain (resi-dues 1 944) tothe cDNA ofthe ... and access tothe receptor.Having established the re lative binding af®nities of IGF-I,IGF-II and the 14 analogues for rhIGF1R, we related these to their abilities to prevent apoptosis. IGFs...
... LY200729 01, L200629 01, 032 912 , 200529 01) , the Youth Science Foundation of Shandong Province (Nos 2005BS 11 010 ), the Natural Science Foundation of Shandong Province (Nos Q2006F05, Y2005F 11) , the ... by the occurrence of some tidy nanoparticles, are as shown in figure 3e. The effect of SnCl4 addition on the morphology of α-Fe2O3 is obvious, but what isthe existence of Sn? it is ... using a JEOL JEM- 210 0F field emission transmis-sion electron microscope. 3. Results and discussion Figure 1 shows the typical XRD pattern ofthe product. All the reflections ofthe XRD pattern...
... are close enough to hydrogen-bond tothe distal ligand. We have proposedthat, if the amide nitrogen of Gly143 isthe proton donorhydrogen-bonded tothe iron-bound oxygen ofthe ferrichydroperoxide, ... stabilize the negatively chargedoxygen and facilitate the electrophilic addition of the terminaloxygentothea-methene bridge [11 ]. The recentfindings by Liu et al. [17 ] that Gly143 mutants, all of whichhave ... additions of 0 (–), 1. 1, 1. 7, 2.6(ÆÆÆ), and 3.3 eq (spectrum a, - - -) of NADPH, in the presence of NADPH-cytochrome P450 reductase, tothe synthetic verdohaemcomplexed with rHO -1. (B) The spectrum...
... Hemingway 15 2How to make great grilled chicken 15 3How to learn to fly fish 15 5How to prevent identity theft 15 8How to compete at altitude 16 0How to watch the Tour de France 16 1How to get rid of ... speaking 18 3How to save the Earth 18 4How to turn a mountain back into a molehill 18 7How to get rid of all those frequent flyer miles 18 8How to live to 10 0 18 9How to run a democracy 19 2How to ... asecurities for this company. banana peel and the other inChapter 11 . Disregard ourexuberance.5 text.p65 1/ 29/2003, 3:32 PM18 11 How to travel to space 11 Hey, if a codger like former Senator John...
... Sacrifice of Happiness? 10 763. Love, Self-Concern, and Concern for Others 10 964. Eudaemonism and Concern for Others 11 16. Aristotle: Happiness 11 465. Interpreting Aristotle 11 466. Aristotle’s ... describe these criticisms as a series of nails in the coffin of Aristoteliannaturalism. According to one view, the criticisms are so cogent that enlightened moralistsare right to discard the Aristotelian ... trying to reconcile Aristotle with Kant. If we try to explore the history of reflexion on Aristotelian naturalism, we may be able to see whether Sidgwick’sview is right. To clarify some ofthe issues...
... PEP -1- SOD1-His or PEP -1- CAT-His had the similar enzymatic activities as the wildtype SOD1 or CAT.Transduction of PEP -1- SOD1 or PEP -1- CAT into H9c2 cell The subcellular transduction of PEP -1- SOD1 ... 9:73http://www.translational-medicine.com/content/9 /1/ 73Page 6 of 11 Figure 3 Effect of PEP -1- SOD1 and PEP -1- CAT on LDH level and apoptosis rate. (A) Effect of PEP -1- SOD1 and PEP -1- CAT on LDH level. *P <0. 01 vs. control (CTL) ... expression wasfurther increased by the treatment with both PEP -1- Figure 1 Transduction of purified PEP -1- SOD1 or PEP -1- CAT into H9c2 cells. A: purific ation of PEP -1- SOD1-His and PEP -1- CAT-His fusionproteins....