factor the sum of cubes calculator

Ngày tải lên: 07/08/2014, 08:20

Ngày tải lên: 07/08/2014, 08:20

Ngày tải lên: 21/06/2014, 01:20

Ngày tải lên: 07/08/2014, 06:22

Ngày tải lên: 07/08/2014, 08:22

... enhance- ment of the expression of the tumour suppressor gene p53 [5]. Because of the important roles of KLF4 on the above three cell types, we postulated the novel effect of KLF4 in atherogenesis. Scavenger ... antibody. (B) Recruitment of KLF4 to the binding element of the SR-BI promoter region. The ChIP assay was used to detect the binding of KLF4 to the SR-BI promoter. The cross-linked protein-DNA ... found that the promoter of SR-BI contained multiple putative KLF4 binding sites. However, the direct effect of KLF4 on the expression of SR-BI remains unknown. Here, the expression of KLF4 in...

Ngày tải lên: 18/02/2014, 04:20

... associated with the generation of heterochromatin, and reduced the binding of HSF1. These results show that HSF4 promotes the DNA-binding activity of another mem- ber of the HSF family, through modulating the ... development of the lens and maintenance of the olfactory epithelium [102]. The first evidence for a developmental function of HSF4 was provided by population genetic studies where mutations of the Hsf4 ... because phosphorylation of serine 303 is a prerequisite for sumoylation, which inhibits the transactivation capac- ity of HSF1 [59–61]. The phosphorylation-dependent sumoylation of HSF1 provided the first example of...

Ngày tải lên: 18/02/2014, 04:20

... minimize the FXa cofactor activity of the reaction intermediate, FVa int . This facilitated the determination of loss of FVa cofactor activity during the initial stage of inactivation and minimized the ... for investigation of the interaction between APC and FVa. However, given the general abundance of heparin-like structures on the surface of the vascular bed, and the lack of good estimates of local concentrations ... indicating that the stimulatory effect of heparin on the cleavage at Arg306 is also observed in the presence of FXa. Theoretically, the addition of heparin may induce the formation of a binary FXa–FXa...

Ngày tải lên: 19/02/2014, 13:20

... polyamine in the regulation of apop- tosis [21,22]. Thus, the possibility that the antiviral effect of spermine is mediated by induction of apop- tosis was analyzed. For this purpose, the effect of spermine ... components of the innate host defense, which act through engagement of specific cell surface receptors and trigger the acti- vation of the janus kinase (JAK) ⁄ signal transducer and activator of transcription ... signaling pathway. Induction of the IFN-stimulated gene (ISG) factor (ISGF)-3 [ISGF3c(IRF-9) ⁄ STAT1 ⁄ STAT2] transcrip- tion factor mediates the induction of a network of Keywords antiviral response;...

Ngày tải lên: 07/03/2014, 21:20

... support of this hypothesis: the dramatic impairment of the oxidative folding in vi- tro induced by the G274D mutation, the misfolding of the G274S and G274A mutants, the impossibility of rescuing the ... whether the fraction of the mutated Jagged-1 that is presented at the cell surface is correctly folded. The results obtained in the present study suggest the opposite. There are several lines of ... point towards the interior of the domain. In other words, any amino acid other than glycine would require a substantial rearrangement of the backbone to reorient the side chain. The experiments...

Ngày tải lên: 16/03/2014, 00:20

... phosphory- lation of the C-terminal SSXS-motif of the R-Smad. Based on the diverse mechanism of Smad activation by either TGF-b1 or NGF, specific subsets of target genes might be induced. The potential of NGF ... 33342 for 2 min and the cells were analysed by confocal microscopy. The projection of multiple sections is seen on the left for each panel to visualize the morphology of the cells. The middle row ... activate the Smad pathway independently of TGF-b might be of special importance in regulating the expression of genes that are essential for the development and function of neuronal cells or other NGF-sensitive...

Ngày tải lên: 16/03/2014, 16:20

... a result of the loss of energetic contributions of alanine- substituted residues rather than the effect of such mutations on protein structure. In summary, the fact that the combined loss of free energy ... accompanying the binding of TRE duplex to various constructs of the Jun-Fos heterodimer were obtained from the fit of a one-site model, based on the binding of a ligand to a macromolecule using the law of ... accompanying the binding of TRE duplex to various constructs of the Jun-Jun heterodimer were obtained from the fit of a one-site model, based on the binding of a ligand to a macromolecule using the law of...

Ngày tải lên: 22/03/2014, 16:20

... Mutation of the octamer in the iNOS and G-CSF promoters results in a reduction of more than 90% in the LPS-induced pro- moter activities of these genes [13,30], suggesting the essential role of the ... might also reduce the expression of these genes. We therefore tested the effect of rapamycin on the LPS-induced expression of iNOS and G-CSF. Fig. 3A shows that detectable levels of iNOS and G-CSF ... inhibition of the LPS-induced increase in iNOS and G-CSF protein levels, supporting the essential role of mTOR. Forced expression of Oct-2 using the pCG–Oct-2 plasmid overcame the inhibitory effect of...

Ngày tải lên: 22/03/2014, 17:20

... packing of TM1 with the other helices, causing a slight shift of TM1 relative to the rest of the bundle (Fig. 3A). At the same time, the main kinks in the TM helices of rhodopsin (e.g. the functionally important ... patterns of homo-oligomeric cross- linking for C5aR and AT 1 R-YFP. Because the rate of cross-linking depends on the proximity of the side groups, the flexibility of the structures containing the cysteine ... determine whether the decrease in binding affinity in the other chimeras was the result of protein instability and misfolding or was merely a byproduct of the localization of chimeras in the ER, cells...

Ngày tải lên: 23/03/2014, 04:21

... respectively, of the receptor [19,22]. The b sheet of EGF may bind to domain I of ErbB-1, and the other surface of the ligand binds to domain III of the receptor. Recently, Gly441 of the ErbB-1 ... of the receptors was confirmed by immunoblotting of the cell lysates (Fig. 1B). As often observed for membrane proteins, the bands were rather broad in the blots, due to the heterogeneity of the ... due to the saturation of ERK activation resulting from the amplifying effect of the signaling cascade. DISCUSSION In the present study, by using chimeric receptors, the contributions of the extracellular...

Ngày tải lên: 24/03/2014, 03:21

... modulate the sensi- tivity in the low micromolar range because the IC 50 value of the dephosphorylated wild-type HRI was increased to 2.4 lm. On the other hand, the IC 50 value (5.5 lm) of the dephosphorylated ... located at the dimer interface and is thus trans-phosphorylated by the kinase domains of other subunits of HRI (Fig. 5B). Unfortunately, because of the low ion score (i.e. a measure of how well the ... other phosphorylation sites in HRI and to establish the contribution of the autophosphorylation process to heme sensing and catalysis. The results obtained should aid the clarifica- tion of the...

Ngày tải lên: 28/03/2014, 23:20

... (designated R4) in the construct F2 resulted in the abolishment of 80% of the relative luciferase activity, despite the presence of exon 1 and the upstream sequence (Fig. 3A). A further deletion ... subsets of genes via the recognition of distinct groups of the con- sensus jB site [30]. In this code, the palindromic 5¢- GGGAATTCCC-3¢ jB sequence of Rnf33 was shown to tolerate a wider range of ... in construct F3 confirmed the importance of R4, albeit with possible further contribution outside the R4 sequence. When one to three copies of the 305-bp R4 sequence were cloned in either the forward or...

Ngày tải lên: 29/03/2014, 00:20

... 71. Considering the location of the N-terminal extremity of DNter2 on the structure of CARP and the presence of the potential cleavage sites, we identified the localization of the cleavage site ... that the loss of one NLS in the C-terminal fragment affects the nuclear transport of this form of CARP, although the sensitiv- ity of the methods we used could not confirm this hypothesis. The ... controlled by the activity of calpain 3: the higher the calpain 3, the more important the sarcomeric retention of CARP. This regulation would occur through cleavage of the N-terminal end of CARP by the...

Ngày tải lên: 29/03/2014, 21:20

... (http://www.rsb.info.nih.gov/ nih-image/), the relative fluorescence intensity of the PTPCs was determined as the intensity of the individual cells relative to the mean pixel fluorescence for the entire somata (S) of the brain. ... analysis of many constructs, enabling the identification of the cis-elements in vivo. Furthermore, the system is highly reproducible and can be set up within 2 weeks of con- struction of the recombinant ... (v[PT ⁄ MEFi]) of the BmMEF2 gene. To obtain v[PT ⁄ MEFs], the BmMEF cDNA corresponding to the open reading frame was inser- ted downstream of the PTTH promoter. The PCR product of the BmMEF2 cDNA...

Ngày tải lên: 30/03/2014, 20:20

... the assessment of the SeP mRNA expression and the measurement of luciferase activity exist. For example, whereas in the first case the species assessed is the mRNA (i.e. the respective cDNA) of ... As the CAGA box at position 21811 is located at the very margin of the known SeP promoter sequence, the 5 0 flanking bases do not represent the context of the genomic promoter but the sequence of ... TGF-b-responsive element in the promoter of the SeP gene. In addition, we found the expression of SeP mRNA being attenuated by overexpression of Smad 3 and 4 which mimics activation of the TbR by TGF-b....

Ngày tải lên: 31/03/2014, 23:20