Ngày tải lên: 07/09/2013, 13:48
Tài liệu Báo cáo " The meaning and structure of a science fiction story: a sysyemic functional analysis " doc
... declarative declarative declarative declarative declarative declarative declarative declarative declarative declarative declarative declarative declarative declarative declarative ... declarative declarative declarative declarative declarative declarative declarative declarative declarative declarative declarative imperative declarative declarative ability/neg. ... anaphoric anaphoric anaphoric exophoric anaphoric exophoric anaphoric anaphoric anaphoric anaphoric exophoric anaphoric anaphoric anaphoric anaphoric exophoric anaphoric anaphoric...
Ngày tải lên: 12/02/2014, 20:20
Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc
... a ⁄ b domain than in the a domain [20]. This is also reflected in the rigidity of the protein. The atomic displacement factors of the PhyK structure are smaller for the a ⁄ b domain than for the ... coli phytase, glucose- 1-phosphatase and human prostatic-acid phosphatase. The polypeptide chain is organized into an a and an a ⁄ b domain, and the active site is located in a positively charged ... substrate-free AppA the C a atoms are 2.41 A ˚ apart, whereas for the substrate-free PhyK and the substrate-loaded AppA the averaged distance is only 1.87 A ˚ . Distinct conformational changes were observed...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf
... because of the absence of the catalytic subunit ISP (Table 1). Figure 3A shows that a band of approximately 500 kDa was also found in this mutant strain when the mitochondrial membranes were ana- lyzed ... dimerization of the bc 1 complex at this stage (i.e. just after the addition of ISP and Qcr10p). On the other hand, in the transition from the 500 kDa band to the 670 kDa band, a structural rearrangement of ... were of analytical grade. Yeast strains and growth media The genotypes and sources of the S. cerevisiae strains are described in Table 2. The ISP deletion strain was prepared in accordance with the...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... that the calcium-binding event of lower affinity is attributable to the catalytic site of PLDa2, as found for PLDb from A. thaliana [39]. The decrease in PLD activity at concentrations above the ... Journal 274 (2007) 2630–2640 ª 2007 The Authors Journal compilation ª 2007 FEBS 2633 from the ratio of the forward scattering intensity of the sample and that of the molecular mass standard BSA. The ab ... 2B). Reconstruction of the overall shape of the PLDa2 from the X-ray scattering data was achieved by the ab initio modeling program dammin [24]. The superpo- sition of the 10 calculated models (Fig. 3) shows a longish...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: ˚ The 1.8 A crystal structure of a proteinase K-like enzyme from a psychrotroph Serratia species docx
... SPRK also has an aspar- tic acid residue at position at 200, and with the same conformation as in the two other structures, but Asp200 forms a salt bridge to Lys253 (Ala253 and Asn250 in PRK and ... [18]. The biophysical characteri- zation of SPRK did not reveal the classical cold adap- ted features [19], but still initial comparative studies showed that the catalytic turnover was at least ... reported. Results and discussion Crystallization, data collection and refinement Crystals of the catalytic domain of the Serratia prote- inase K (SPRK) grew within a few days to thin hexa- gonal plates growing...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: "The Utility of a Graphical Representation of Discourse Structure in Spoken Dialogue Systems" ppt
... system. Also, their underlying task (air travel domain) is much simpler than our tutoring task. In addition, the SIH is not always available and users have to activate it manually. Other visual ... hints/de- scribes the answer, each discourse segment has an additional purpose annotation that is displayed when the segment is part of the visible horizon. Auto-collapse. To reduce the amount of infor- mation ... also have an equivalent in a new domain (e.g. displaying the recognized user answer can be the equivalent of the correct answers). Other NM usages can also be imagined: e.g. reducing the length...
Ngày tải lên: 20/02/2014, 12:20
Báo cáo khoa học: Structure of Streptococcus agalactiae serine⁄threonine phosphatase The subdomain conformation is coupled to the binding of a third metal ion pptx
... Hospital and Regional Medical Center, Seattle, WA, USA Protein phosphatases are primarily classified on the basis of the type of the amino acid they dephosphory- late, serine ⁄ threonine phosphatases ... fused to an EF-hand motif, and human STP (HsSTP), which has an additional 8 kDa a- helical domain at the C-terminus [3,4]. The sizes of the catalytic domains of both PPP and PPM families are well ... kinase and adenylosuccinate syn- thase, but not in S. agalactiae CovR ⁄ CsrR. The motif is located at the surface of the SaPPase (Rantanen, unpublished), and superposition of the prolines allows superposition...
Ngày tải lên: 07/03/2014, 09:20
Báo cáo khoa học: Structure of the complex of a yeast glucoamylase with acarbose reveals the presence of a raw starch binding site on the catalytic domain doc
... GLU H44 7A forward (5¢- GCAAGTCATTT TGGATGCTATTAATGATGATGGCTC-3¢), GLU H44 7A reverse (5¢- GAGCCATCATCATTAATAGCATCCAAAA TGACTTGC-3¢); GLU T46 2A forward (5¢- GAACAACTT AACAGATATGCCGGTTATT CCACCGGT ... as a template. The following oligonucleotides were used: GLU R1 5A forward (5¢-ATTCAAACTATAAAGTTGACGCAA CTGACTTGGAAACCTTC-3¢), GLU R1 5A reverse (5¢- GAAGGTTTCCAAGTCAGTTGCGTCAACTTTATAGTT TGAAT-3¢); ... GLU T46 2A reverse (5¢- GGCACCGGTGGAATAACCGGCA TATCTGTTAAGTTGTTC-3¢); GLU H44 7A, D45 0A for- ward (5¢-GCAAGTCATTTTGGATGCTATTAATGCTG ATGGCTCCTTGAATGAAC-3¢), GLU H44 7A, D45 0A Fig. 7. Adsorption to raw...
Ngày tải lên: 07/03/2014, 12:20
Inside the Structure of Defined Contribution/401(k) Plan Fees: A Study Assessing the Mechanics of the ‘All-In’ Fee pot
... In the report, Exhibit 3 plots the impact of average account balance and number of plan participants on the ‘all-in’ fee for a variety of combinations of average account balance and number of ... assets to arrive at the ‘all-in’ fee as a percentage of plan assets. Also, each plan’s total dollar fee amount was divided by total participants in the plan to arrive at the ‘all-in’ fee as ... further analysis found that a core specification that allowed average account balances and number of participants (both in logs) to affect fees as a percentage of assets each had separate and...
Ngày tải lên: 07/03/2014, 13:20
Báo cáo khoa học: Unravelling the functional interaction structure of a cellular network from temporal slope information of experimental data docx
... excluding the INS at x i obtained at Step 3 from the ANS at x i and thereby we can identify the true interaction structure at x i (Supplementary material, Supplementary mathematical descriptions, Theorem ... however, only applicable to the case when for each node there are as many parameters as the number of overall network nodes and these parameters do not directly affect the corresponding node. The fundamental ... chosen n parameter perturbations. All these approaches are based on parameter perturbation experiments which are, however, not always achievable in many practical cases. In this paper, we therefore...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: The oxidative effect of bacterial lipopolysaccharide on native and cross-linked human hemoglobin as a function of the structure of the lipopolysaccharide A comparison of the effects of smooth and rough lipopolysaccharide ppt
... to an increase in the rate of the initial fast phase, i.e. oxidation of the a chains. The rates of oxidation are reduced in the presence of chelators of heavy metal cations in most cases. An ... 3A. The rate of auto-oxidation of cross-linked Hb was greater than that of Hb A 0, both in the presence and absence of EDTA, as has been observed previously [17]. In addition, the rates of auto-oxidation ... binding of the Hb tetramer to the LPS molecule makes the heme cavity of the a chains more accessible to a water molecule which can then accelerate the displacement of the protonated superoxide anion,...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo Y học: Electrostatic properties of the structure of the docking and dimerization domain of protein kinase A IIa doc
... demonstrate the presence of positive charge. Figure 4I shows the bottom face (same as in Fig. 4C) of a van der Waals model of the best structure of RIIa D/D. The central part of this face appears ... two basic sites, the N-terminal amide and His2. Aspartic acids Asp27 and Asp30 show lower mean pK a values than their model pK a values. This may be a general property of Asp residues, as it has ... To examine the electrostatic character of the structure of RIIa D/D we have predicted mean apparent pK a values, based on Poisson–Boltzmann electrostatic calculations, using an ensemble of calculated...
Ngày tải lên: 08/03/2014, 22:20
Báo cáo khoa học: "SOFTWARE TOOLS FOR THE ENVIRONMENT OF A COMPUTER AIDED TRANSLATION SYSTEM" pptx
... factors : the dispersal of infor- mation and the obscurity of the coding. In ARIANE-78, the lexical data base may reside on much more 50 files, for a given pair of language. This data base ... part of another file, make permutations or transpositions of several parts of a file, and receive suggestions for the translation of a word displayed in a win- dow. Moreover, the system can ... always done by the translator. THAM consists of a set of functions programmed in the macro language associated with a powerful text editor. These functions help the translator and improve his...
Ngày tải lên: 17/03/2014, 19:21
Báo cáo khoa học: Structure of a trypanosomatid mitochondrial cytochrome c with heme attached via only one thioether bond and implications for the substrate recognition requirements of heme lyase potx
... compilation ª 2009 FEBS 2831 of the alanine of the AXXCH motif (Ala25) (in green) and the unsaturated vinyl group of the heme (cyan) are separated by 3.41 A ˚ (as compared with a typical thioether ... 72% similarity. The structures are remarkably similar overall, and in the details of the heme attachment and heme position. The rmsd between the structure of C. fasciculata cytochrome c and S. ... Crystallographic data were collected at beamline IO2 at Diamond Light Source, UK, and we acknowledge the support of T. Sorensen at Diamond and A. Labrador at the ESRF, France. References 1 Barker...
Ngày tải lên: 23/03/2014, 04:21
Báo cáo khoa học: The crystal structure of a hyperthermostable subfamily II isocitrate dehydrogenase from Thermotoga maritima pdf
Ngày tải lên: 23/03/2014, 10:21