the structure of a diary entry

Ngày tải lên: 01/04/2014, 12:20

Ngày tải lên: 12/08/2014, 17:20

Ngày tải lên: 07/09/2013, 13:48

... declarative declarative declarative declarative declarative declarative declarative declarative declarative declarative declarative declarative declarative declarative declarative ... declarative declarative declarative declarative declarative declarative declarative declarative declarative declarative declarative imperative declarative declarative ability/neg. ... anaphoric anaphoric anaphoric exophoric anaphoric exophoric anaphoric anaphoric anaphoric anaphoric exophoric anaphoric anaphoric anaphoric anaphoric exophoric anaphoric anaphoric...

Ngày tải lên: 12/02/2014, 20:20

... a ⁄ b domain than in the a domain [20]. This is also reflected in the rigidity of the protein. The atomic displacement factors of the PhyK structure are smaller for the a ⁄ b domain than for the ... coli phytase, glucose- 1-phosphatase and human prostatic-acid phosphatase. The polypeptide chain is organized into an a and an a ⁄ b domain, and the active site is located in a positively charged ... substrate-free AppA the C a atoms are 2.41 A ˚ apart, whereas for the substrate-free PhyK and the substrate-loaded AppA the averaged distance is only 1.87 A ˚ . Distinct conformational changes were observed...

Ngày tải lên: 16/02/2014, 09:20

... because of the absence of the catalytic subunit ISP (Table 1). Figure 3A shows that a band of approximately 500 kDa was also found in this mutant strain when the mitochondrial membranes were ana- lyzed ... dimerization of the bc 1 complex at this stage (i.e. just after the addition of ISP and Qcr10p). On the other hand, in the transition from the 500 kDa band to the 670 kDa band, a structural rearrangement of ... were of analytical grade. Yeast strains and growth media The genotypes and sources of the S. cerevisiae strains are described in Table 2. The ISP deletion strain was prepared in accordance with the...

Ngày tải lên: 18/02/2014, 08:20

... that the calcium-binding event of lower affinity is attributable to the catalytic site of PLDa2, as found for PLDb from A. thaliana [39]. The decrease in PLD activity at concentrations above the ... Journal 274 (2007) 2630–2640 ª 2007 The Authors Journal compilation ª 2007 FEBS 2633 from the ratio of the forward scattering intensity of the sample and that of the molecular mass standard BSA. The ab ... 2B). Reconstruction of the overall shape of the PLDa2 from the X-ray scattering data was achieved by the ab initio modeling program dammin [24]. The superpo- sition of the 10 calculated models (Fig. 3) shows a longish...

Ngày tải lên: 19/02/2014, 02:20

... SPRK also has an aspar- tic acid residue at position at 200, and with the same conformation as in the two other structures, but Asp200 forms a salt bridge to Lys253 (Ala253 and Asn250 in PRK and ... [18]. The biophysical characteri- zation of SPRK did not reveal the classical cold adap- ted features [19], but still initial comparative studies showed that the catalytic turnover was at least ... reported. Results and discussion Crystallization, data collection and refinement Crystals of the catalytic domain of the Serratia prote- inase K (SPRK) grew within a few days to thin hexa- gonal plates growing...

Ngày tải lên: 19/02/2014, 07:20

... system. Also, their underlying task (air travel domain) is much simpler than our tutoring task. In addition, the SIH is not always available and users have to activate it manually. Other visual ... hints/de- scribes the answer, each discourse segment has an additional purpose annotation that is displayed when the segment is part of the visible horizon. Auto-collapse. To reduce the amount of infor- mation ... also have an equivalent in a new domain (e.g. displaying the recognized user answer can be the equivalent of the correct answers). Other NM usages can also be imagined: e.g. reducing the length...

Ngày tải lên: 20/02/2014, 12:20

... Hospital and Regional Medical Center, Seattle, WA, USA Protein phosphatases are primarily classified on the basis of the type of the amino acid they dephosphory- late, serine ⁄ threonine phosphatases ... fused to an EF-hand motif, and human STP (HsSTP), which has an additional 8 kDa a- helical domain at the C-terminus [3,4]. The sizes of the catalytic domains of both PPP and PPM families are well ... kinase and adenylosuccinate syn- thase, but not in S. agalactiae CovR ⁄ CsrR. The motif is located at the surface of the SaPPase (Rantanen, unpublished), and superposition of the prolines allows superposition...

Ngày tải lên: 07/03/2014, 09:20

... GLU H44 7A forward (5¢- GCAAGTCATTT TGGATGCTATTAATGATGATGGCTC-3¢), GLU H44 7A reverse (5¢- GAGCCATCATCATTAATAGCATCCAAAA TGACTTGC-3¢); GLU T46 2A forward (5¢- GAACAACTT AACAGATATGCCGGTTATT CCACCGGT ... as a template. The following oligonucleotides were used: GLU R1 5A forward (5¢-ATTCAAACTATAAAGTTGACGCAA CTGACTTGGAAACCTTC-3¢), GLU R1 5A reverse (5¢- GAAGGTTTCCAAGTCAGTTGCGTCAACTTTATAGTT TGAAT-3¢); ... GLU T46 2A reverse (5¢- GGCACCGGTGGAATAACCGGCA TATCTGTTAAGTTGTTC-3¢); GLU H44 7A, D45 0A for- ward (5¢-GCAAGTCATTTTGGATGCTATTAATGCTG ATGGCTCCTTGAATGAAC-3¢), GLU H44 7A, D45 0A Fig. 7. Adsorption to raw...

Ngày tải lên: 07/03/2014, 12:20

... In the report, Exhibit 3 plots the impact of average account balance and number of plan participants on the ‘all-in’ fee for a variety of combinations of average account balance and number of ... assets to arrive at the ‘all-in’ fee as a percentage of plan assets. Also, each plan’s total dollar fee amount was divided by total participants in the plan to arrive at the ‘all-in’ fee as ... further analysis found that a core specification that allowed average account balances and number of participants (both in logs) to affect fees as a percentage of assets each had separate and...

Ngày tải lên: 07/03/2014, 13:20

... excluding the INS at x i obtained at Step 3 from the ANS at x i and thereby we can identify the true interaction structure at x i (Supplementary material, Supplementary mathematical descriptions, Theorem ... however, only applicable to the case when for each node there are as many parameters as the number of overall network nodes and these parameters do not directly affect the corresponding node. The fundamental ... chosen n parameter perturbations. All these approaches are based on parameter perturbation experiments which are, however, not always achievable in many practical cases. In this paper, we therefore...

Ngày tải lên: 07/03/2014, 21:20

... to an increase in the rate of the initial fast phase, i.e. oxidation of the a chains. The rates of oxidation are reduced in the presence of chelators of heavy metal cations in most cases. An ... 3A. The rate of auto-oxidation of cross-linked Hb was greater than that of Hb A 0, both in the presence and absence of EDTA, as has been observed previously [17]. In addition, the rates of auto-oxidation ... binding of the Hb tetramer to the LPS molecule makes the heme cavity of the a chains more accessible to a water molecule which can then accelerate the displacement of the protonated superoxide anion,...

Ngày tải lên: 08/03/2014, 10:20

... demonstrate the presence of positive charge. Figure 4I shows the bottom face (same as in Fig. 4C) of a van der Waals model of the best structure of RIIa D/D. The central part of this face appears ... two basic sites, the N-terminal amide and His2. Aspartic acids Asp27 and Asp30 show lower mean pK a values than their model pK a values. This may be a general property of Asp residues, as it has ... To examine the electrostatic character of the structure of RIIa D/D we have predicted mean apparent pK a values, based on Poisson–Boltzmann electrostatic calculations, using an ensemble of calculated...

Ngày tải lên: 08/03/2014, 22:20

... compilation ª 2009 FEBS 2831 of the alanine of the AXXCH motif (Ala25) (in green) and the unsaturated vinyl group of the heme (cyan) are separated by 3.41 A ˚ (as compared with a typical thioether ... 72% similarity. The structures are remarkably similar overall, and in the details of the heme attachment and heme position. The rmsd between the structure of C. fasciculata cytochrome c and S. ... Crystallographic data were collected at beamline IO2 at Diamond Light Source, UK, and we acknowledge the support of T. Sorensen at Diamond and A. Labrador at the ESRF, France. References 1 Barker...

Ngày tải lên: 23/03/2014, 04:21

... Reykjavik, Iceland), and were refined by varying the pH of the buffer and the concentration of the precipi- tant. Prior to data collection, a crystal was transferred into Paratone-N (Hampton Research) ... used as a search model. The cns program [19] was used for refinement against the 20–2.5 A ˚ intensity data. A ran- domly selected portion of the diffraction data (5.0%) was used to calculate the ... kinetic parameters The kinetic parameters were determined at various concen- trations of the substrate, tamarind seed xyloglucan (Dainip- pon Sumitomo Pharma, Osaka, Japan), in 50 mm sodium acetate...

Ngày tải lên: 23/03/2014, 05:22

... D36N-1 5’-CATCCTTCCCTATCTC AACATCCAGCTGGTTTACT-3’ D341N-1 5’-AAGGGGAGAACTC AACGGAACACCGGAGG-3’ D389N 5’-CACTCTCGAAGAGTTCATA AACGAAGTGAAGAAGAATCTC-3’ 5’-GAGATTCTTCTTCACTTCGT TTATGAACTCTTCGAGAGTG-3’ M. Karlstro ¨ m ... 5’-CCTGGAGAAATCGATCA TGAGCTTCGCTCAGTCGTG-3’ 5’-CACGACTGAGCGAAGCT CATGATCGATTTCTCCAGG-3’ F205M 5’-AAAAGGTCGACATCTGG ATGGCGACGAAAGACACGATC-3’ 5’-GATCGTGTCTTTCGTCGC CATCCAGATGTCGACCTTTT-3’ D36N ... based on the C a -atom coordinates and secondary structural assignments made using the program dssp [72]. Surface and volume calculations and analysis of noncovalent interactions Ion pairs and...

Ngày tải lên: 23/03/2014, 10:21

Ngày tải lên: 23/03/2014, 13:20

Ngày tải lên: 24/03/2014, 04:21