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Báo cáo khoa học: The role of residues R97 and Y331 in modulating the pH optimum of an insect b-glycosidase of family 1 pdf

Báo cáo khoa học: The role of residues R97 and Y331 in modulating the pH optimum of an insect b-glycosidase of family 1 pdf

... result from the modifications in the interactions between the catalytic glutamates and the residues R97 and Y331, indicating that these residues play a role in the modulation of the Sfbgly50 pH optimum. Sequence ... m M Bicine buffer 7.0–8.5). The pH stability of Sfbgly50 was checked by incubation in the same buffers for a time equal to the assay time and then determining the activity remaining at the optimum ... [10,12]. Nevertheless, it is not possible to assume that the same is occurring in the mutant R97M, because there is no data on the interactions between the substrate and the arginine residue. Finally, another...

Ngày tải lên: 23/03/2014, 15:21

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Báo cáo khoa học: The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B1 and B2 bradykinin receptors potx

Báo cáo khoa học: The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B1 and B2 bradykinin receptors potx

... by the amount of internalized agonist [20]. As the internalization is expressed in percentage of total binding, decreasing the binding affinity of the Role of helix 8 and C-termini in bradykinin ... 2004 FEBS The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B 1 and B 2 bradykinin receptors Alexander Faussner 1 , Alexandra Bauer 1 , Irina Kalatskaya 1 , ... addition of scintillation fluid. Nonspecific binding was determined in the presence of 5 lm unlabeled agonist and subtracted from the total binding to calculate the spe- cific binding. Internalization of...

Ngày tải lên: 07/03/2014, 16:20

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Báo cáo khoa học: Use of lithium and SB-415286 to explore the role of glycogen synthase kinase-3 in the regulation of glucose transport and glycogen synthase pdf

Báo cáo khoa học: Use of lithium and SB-415286 to explore the role of glycogen synthase kinase-3 in the regulation of glucose transport and glycogen synthase pdf

... of GSK3 (using Li or SB-415286) appears to be sufficient for inducing activation of GS in muscle and fat cells, and that inhibition of the kinase potentiates insulin action in muscle of insulin-resistant ... increases in glucose uptake and glycogen synthesis allowing for more effective ÔchannellingÕ of glucose into glycogen in response to insulin. However, one of the potential difficulties in interpreting ... phosphatidylinositol 3-kinase, p70, S6 kinase and glycogen synthase kinase-3 activity in L6 muscle cells: evidence for the involvement of the mammalian target of rapamycin (mTOR) pathway in the L -leucine-induced up-regulation...

Ngày tải lên: 08/03/2014, 08:20

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Báo cáo Y học: The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase pdf

Báo cáo Y học: The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase pdf

... phenylalanine was therefore mutated to Ala, Leu, Trp or Tyr. These mutations may in uence the shape and volume of the acyl binding pocket and thereby alter the binding mode and affinity of the enzyme ... PAA and derivatives thereof, while maintaining the hydrophobicity of the binding site. To test the effect of the mutations on the specificity for phenylacetylated substrates, the steady-state kinetic ... k cat represents the rate of acylation and K m equals the binding constant of the substrate to the free enzyme. The results then indicate Ó FEBS 2002 Role of active-site phenylalanines in penicillin acylase...

Ngày tải lên: 17/03/2014, 23:20

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Báo cáo khoa học: Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability potx

Báo cáo khoa học: Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability potx

... unsure of the mechanism of DNA-induced stabilization of the protein, and whe- ther it is related to the normal RNA-binding function of coat protein, as RNA binding had no effect. The increased ... understood, and we have investigated these questions in this work. We sought to determine the role of protein–RNA and protein–protein interactions in virus stability, measuring the effects of urea, GdnHCl ... against all the forms of denatura- tion we tested. This is consistent with the observation of increased stability of the single-chain version of the wild-type dimer [30] and is reminiscent of the...

Ngày tải lên: 30/03/2014, 11:20

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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pptx

báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pptx

... visualized the mineralizing cells with 2% Alizarin Red. After rinsing in distilled water and air drying the samples, we mounted the cov- erslips on microscope slides using Fluoromount and examined and ... result in loss of ambulation or limb. Here we describe the sequential roles of FGF-2 in inducing gene expression, cell growth and BMP-2 in gene expression and mineralization of bone. Materials and ... BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP- 2 on fold increase in abundance of mineralization-related gene expression. Mineralizing...

Ngày tải lên: 20/06/2014, 04:20

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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pdf

báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pdf

... Li 4 Abstract Introduction: The difficulty in re-growing and mineralizing new bone after severe fracture can result in loss of ambulation or limb. Here we describe the sequential roles of FGF-2 in inducing ... visualized the mineralizing cells with 2% Alizarin Red. After rinsing in distilled water and air drying the samples, we mounted the cov- erslips on microscope slides using Fluoromount and examined and ... BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP- 2 on fold increase in abundance of mineralization-related gene expression. Mineralizing...

Ngày tải lên: 20/06/2014, 07:20

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Báo cáo khoa học: "The role of Bcl-xL and nuclear factor-kB in the effect of taxol on the viability of dendritic cells" ppsx

Báo cáo khoa học: "The role of Bcl-xL and nuclear factor-kB in the effect of taxol on the viability of dendritic cells" ppsx

... rationale for the use of taxol in DC immunotherapy and insights into the development of combinational DC therapies with other anticancer drugs that do not harm the viability or activity of DCs. Acknowledgments ... cells, producing major cytokines involved in immune responses. Importantly, taxol increased the expression of the anti-apoptotic protein Bcl-xL, and increased the mobilization of p65 subunit ... jooh@jejunu.ac.kr The role of Bcl-xL and nuclear factor- κ B in the effect of taxol on the viability of dendritic cells Mi-Hyoung Kim 1 , Hong-Gu Joo 1,2, * 1 Laboratory of Veterinary Pharmacology, College of...

Ngày tải lên: 07/08/2014, 23:22

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