the role of teachers schools and communities in quality education
Báo cáo khoa học: The role of residues R97 and Y331 in modulating the pH optimum of an insect b-glycosidase of family 1 pdf
Ngày tải lên :
23/03/2014, 15:21
... result
from the modifications in the interactions between the
catalytic glutamates and the residues R97 and Y331,
indicating that these residues play a role in the modulation
of the Sfbgly50 pH optimum.
Sequence ... m
M
Bicine buffer 7.0–8.5). The
pH stability of Sfbgly50 was checked by incubation in the
same buffers for a time equal to the assay time and then
determining the activity remaining at the optimum ... [10,12].
Nevertheless, it is not possible to assume that the same is
occurring in the mutant R97M, because there is no data on
the interactions between the substrate and the arginine
residue.
Finally, another...
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Báo cáo khoa học: The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B1 and B2 bradykinin receptors potx
Ngày tải lên :
07/03/2014, 16:20
... by the amount of internalized agonist
[20]. As the internalization is expressed in percentage
of total binding, decreasing the binding affinity of the
Role of helix 8 and C-termini in bradykinin ... 2004 FEBS
The role of helix 8 and of the cytosolic C-termini in
the internalization and signal transduction of B
1
and B
2
bradykinin receptors
Alexander Faussner
1
, Alexandra Bauer
1
, Irina Kalatskaya
1
, ... addition of scintillation fluid. Nonspecific binding
was determined in the presence of 5 lm unlabeled agonist
and subtracted from the total binding to calculate the spe-
cific binding.
Internalization of...
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Báo cáo khoa học: Use of lithium and SB-415286 to explore the role of glycogen synthase kinase-3 in the regulation of glucose transport and glycogen synthase pdf
Ngày tải lên :
08/03/2014, 08:20
... of GSK3 (using Li or SB-415286)
appears to be sufficient for inducing activation of GS in
muscle and fat cells, and that inhibition of the kinase
potentiates insulin action in muscle of insulin-resistant ... increases in glucose uptake and glycogen synthesis
allowing for more effective ÔchannellingÕ of glucose into
glycogen in response to insulin. However, one of the
potential difficulties in interpreting ... phosphatidylinositol
3-kinase, p70, S6 kinase and glycogen synthase kinase-3 activity in
L6 muscle cells: evidence for the involvement of the mammalian
target of rapamycin (mTOR) pathway in the
L
-leucine-induced
up-regulation...
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Báo cáo Y học: The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase pdf
Ngày tải lên :
17/03/2014, 23:20
... phenylalanine was therefore
mutated to Ala, Leu, Trp or Tyr. These mutations may
in uence the shape and volume of the acyl binding pocket
and thereby alter the binding mode and affinity of the
enzyme ... PAA and derivatives thereof, while maintaining
the hydrophobicity of the binding site.
To test the effect of the mutations on the specificity for
phenylacetylated substrates, the steady-state kinetic ... k
cat
represents
the rate of acylation and K
m
equals the binding constant of
the substrate to the free enzyme. The results then indicate
Ó FEBS 2002 Role of active-site phenylalanines in penicillin acylase...
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Báo cáo khoa học: Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability potx
Ngày tải lên :
30/03/2014, 11:20
... unsure of the mechanism
of DNA-induced stabilization of the protein, and whe-
ther it is related to the normal RNA-binding function
of coat protein, as RNA binding had no effect.
The increased ... understood, and we have investigated these
questions in this work. We sought to determine the
role of protein–RNA and protein–protein interactions
in virus stability, measuring the effects of urea,
GdnHCl ... against all the forms of denatura-
tion we tested. This is consistent with the observation
of increased stability of the single-chain version of the
wild-type dimer [30] and is reminiscent of the...
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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pptx
Ngày tải lên :
20/06/2014, 04:20
... visualized the mineralizing
cells with 2% Alizarin Red. After rinsing in distilled
water and air drying the samples, we mounted the cov-
erslips on microscope slides using Fluoromount and
examined and ... result in loss of
ambulation or limb. Here we describe the sequential roles of FGF-2 in inducing gene expression, cell growth and
BMP-2 in gene expression and mineralization of bone.
Materials and ... BMP-2, the yin and yang of mineralization:
Contrast of effect of 24 hours of treatment with FGF-2 or BMP-
2 on fold increase in abundance of mineralization-related gene
expression. Mineralizing...
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- 0
báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pdf
Ngày tải lên :
20/06/2014, 07:20
... Li
4
Abstract
Introduction: The difficulty in re-growing and mineralizing new bone after severe fracture can result in loss of
ambulation or limb. Here we describe the sequential roles of FGF-2 in inducing ... visualized the mineralizing
cells with 2% Alizarin Red. After rinsing in distilled
water and air drying the samples, we mounted the cov-
erslips on microscope slides using Fluoromount and
examined and ... BMP-2, the yin and yang of mineralization:
Contrast of effect of 24 hours of treatment with FGF-2 or BMP-
2 on fold increase in abundance of mineralization-related gene
expression. Mineralizing...
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- 0
Báo cáo khoa học: "The role of Bcl-xL and nuclear factor-kB in the effect of taxol on the viability of dendritic cells" ppsx
Ngày tải lên :
07/08/2014, 23:22
...
rationale for the use of taxol in DC immunotherapy and
insights into the development of combinational DC
therapies with other anticancer drugs that do not harm the
viability or activity of DCs.
Acknowledgments
... cells, producing major
cytokines involved in immune responses. Importantly,
taxol increased the expression of the anti-apoptotic protein
Bcl-xL, and increased the mobilization of p65 subunit ... jooh@jejunu.ac.kr
The role of Bcl-xL and nuclear factor-
κ
B in the effect of taxol on the
viability of dendritic cells
Mi-Hyoung Kim
1
, Hong-Gu Joo
1,2,
*
1
Laboratory of Veterinary Pharmacology, College of...
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- 0
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- 2005 the role of teachers schools and communities in quality education a review of the literature academy for educational development global education center
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