the nonexposed variant of bisphosphonate induced onj
báo cáo hóa học: " Activation of retinal microglia rather than microglial cell density correlates with retinal neovascularization in the mouse model of oxygen-induced retinopathy" ppt
Ngày tải lên :
19/06/2014, 22:20
... in the formation of retinal NV in the later stages of the OIR model In this study, we investigated the temporal and spatial distribution of microglial cells during the different stages of the ... retina, the mean number of microglial cells Page of from the three fields of each counting position (central or peripheral zone, and layer) was calculated Then, the mean and standard error of these ... expressed under the control of the Cx3cr1 promoter They are located in the vitreous (v) near the retina (see arrow heads in the cryosection) In contrast to the microglial cells, they have a larger...
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báo cáo hóa học:" The Edinburgh variant of a talar body fracture: a case report" docx
Ngày tải lên :
20/06/2014, 04:20
... artery supplying the talar body [17,18] The prognosis of talar fractures/dislocations is related to the severity of the injury, length of time before relocation and early fixation The infection ... of the talus: experience of two level trauma centers Foot Ankle Int 2000, 21:1023-9 Higgins TF, Baumgaertner MR: Diagnosis and treatment of fractures of the talus: a comprehensive review of the ... included [19] The majority infections occur after an open fracture which carries a worse prognosis [20] The risk of avascular necrosis of the talar body is related to the type of fracture, with...
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báo cáo hóa học:" The Edinburgh variant of a talar body fracture: a case report" doc
Ngày tải lên :
20/06/2014, 07:20
... artery supplying the talar body [17,18] The prognosis of talar fractures/dislocations is related to the severity of the injury, length of time before relocation and early fixation The infection ... of the talus: experience of two level trauma centers Foot Ankle Int 2000, 21:1023-9 Higgins TF, Baumgaertner MR: Diagnosis and treatment of fractures of the talus: a comprehensive review of the ... included [19] The majority infections occur after an open fracture which carries a worse prognosis [20] The risk of avascular necrosis of the talar body is related to the type of fracture, with...
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Chapter 18 - Lithium Batteries: The Latest Variant of Portable Electrical Energy pdf
Ngày tải lên :
05/07/2014, 07:20
... helium It is the most lightweight of the lightweight metals, the alkali metals According to the rules of chemistry it behaves similarly as the other metals of the same column of the periodic ... with the model of the molecular orbital (MO) and band theory10 The oxidation potential of the electrolyte has to be higher than the potential of the anode (or than the Fermi energy of the anodic ... structure – as long as there is no disorder within the planes The geometry of the edges is either of the ‘arm chair’ or of the ‘zigzag’ type (Figure 18.36) Here the carbon atoms themselves are also...
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Báo cáo y học: "Cartilage oligomeric matrix protein deficiency promotes early onset and the chronic development of collagen-induced arthritis" pdf
Ngày tải lên :
09/08/2014, 13:22
... in the acute stage of the disease [24] Thus, the absence of one cartilage protein can affect how effector molecules of the immune system reach and bind other cartilage proteins To test whether ... 13 14 15 The authors declare that they have no competing interests Authors' contributions HG was responsible for the majority of the practical work and the writing of the manuscript The study ... approved the animal experiments described in this article Induction and evaluation of collagen II -induced arthritis The mice were injected subcutaneously at the base of the tail with 100 μg of rat...
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Báo cáo y học: "Distinct signal transduction processes by IL-4 and IL-13 and influences from the Q551R variant of the human IL-4 receptor alpha chain" docx
Ngày tải lên :
12/08/2014, 18:20
... al ing the unique effects of IL-13 on activated STAT3 The functions of IL-4 and IL-13 are further influenced by some of the IL-4Rα variants These findings may also have implications for the development ... for the common IL-4Rα variants I50V, E375A, S478P and Q551R [15,16] Those individuals bearing the intracellular R551 variant of the IL4α (homozygous R551 = variant) and no other intracellular variant, ... bore the extracellular variant I50V The frequency of individuals showing only the R551 variant and no other polymorphism in IL-4Rα is less than 1% in the German population (unpublished data) As the...
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The Molecular Mechanism of Break Induced Replication
Ngày tải lên :
24/08/2014, 13:24
... Disclaimer Title of Thesis/Dissertation: The Molecular Mechanism of Break Induced Replication For the degree of Master of Science Choose your degree I certify that in the preparation of this thesis, I ... The Molecular Mechanism of Break Induced Replication Major Professor: Anna Malkova DNA double strand break (DSB) is one of the most threatening of all types of ... resolution After invasion of one side of DSB followed by DNA synthesis, the duplex molecule dissociates and re-‐anneals to the other side of the lesion, making crossover...
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Báo cáo y học: "RANKL increases the level of Mcl-1 in osteoclasts and reduces bisphosphonate-induced osteoclast apoptosis in vitro" pps
Ngày tải lên :
09/08/2014, 14:20
... the loss of Mcl-1 preceded the appearance of morphological and biochemical features of apoptosis and occurred in the absence of changes in the level of Bcl-2 or Bcl-xL In addition, the loss of ... osteoclasts in cultures in the absence of BPs RANKL protects osteoclasts from the anti-resorptive effects of bisphosphonates The effect of RANKL on the morphology of BP-treated rabbit osteoclasts ... Mcl-1 The ability of RANKL, together with other factors such as TNF- [23], to rescue osteoclasts from the pro-apoptotic effects of BPs may account for the apparent lack of effectiveness of BPs...
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Tài liệu Báo cáo khoa học: Salt-induced formation of the A-state of ferricytochrome c – effect of the anion charge on protein structure docx
Ngày tải lên :
19/02/2014, 05:20
... coordinated form The reduced effect of Cl– reflects the different affinities of the two anions for the protein [11,17] We also monitored the effect of sulfate on the perchlorate -induced A-state ... comparison of the kinetic behavior of the mutants with that of the acid-denatured protein in the reaction with sulfate (Fig 9B) As mentioned above, at 395 nm the (sulfate -induced) refolding kinetics of ... been marked, for the sake of image clarity the positively charged clusters located on the protein surface [11] The distribution of lysines around the heme crevice at the ‘front’ of the molecule is...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
Ngày tải lên :
19/02/2014, 13:20
... independent of the thermodynamic and kinetic properties of the binding process and is solely based on the detectability of the peptides of interest We have used this method to show that binding of fosfomycin ... function of temperature [13] The heat capacity change (DCp) was obtained from the slope of Kirchoff’s plots (DH vs T) Analysis of the experimental DCp with DCp calculated from the change of solvent ... replacement of R120 by lysine reduces the probability of forming the fully closed form of the protein The results obtained with the K22V/R120K double mutant protein point toward a central role of arginine...
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Báo cáo khoa học: Binding of ATP at the active site of human pancreatic glucokinase – nucleotide-induced conformational changes with possible implications for its kinetic cooperativity doc
Ngày tải lên :
06/03/2014, 00:20
... indicated by the MD simulations of the modelled ternary hGK–Glc– ATP complex In the final structure of the simulations, the interactions of the adenosine moiety were similar to those observed in the binary ... Fig The Glc binding isotherm for the mutant L146R GST–hGK The enhancement of ITF was measured at 25 °C with increasing concentrations of Glc in the absence (d) and presence (s) of 2.5 mM ATPcS The ... ATP-binding site in the MD simulated model structure of the binary hGK–ATP complex and the domain motion induced by ATP binding to the hGK apoenzyme (A) Close-up view of the ATPbinding site in the MD simulated...
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Báo cáo khoa học: SAF-3, a novel splice variant of the SAF-1/MAZ/Pur-1 family, is expressed during inflammation pptx
Ngày tải lên :
07/03/2014, 02:20
... in the unique N-terminus, (b) binding of the N-terminus by ancillary factors, (c) the lack of a negatively functioning module that is present in the N-terminus of SAF-1 [26], or (d) all of the ... addition, methylation of the cytosines of CpG islands in the promoter or the first exon has been shown to affect the rate of transcription [33], and ever since the clear demonstration of a causal relationship ... matches the translation initiation site consensus sequence as determined by Kozak [25] (Fig 2B) The second exon of SAF-3 (exon 1C) starts at around the middle of the first exon of SAF-1/SAF-2 The...
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Báo cáo khoa học: Inhibition of PI3K/Akt partially leads to the inhibition of PrPC-induced drug resistance in gastric cancer cells pdf
Ngày tải lên :
07/03/2014, 03:20
... exhibiting IR; 3, 50–75% of the tumor cells exhibiting IR; 4, > 75% of the tumor cells exhibiting IR The overall score was the multiplication of the two The scores were then classified as negative ... operating at various steps in the cytotoxic activity of the drug, from a decrease of drug accumulation in the cell to abrogation of apoptosis induced by the chemical substance Often several different ... immunoreactivity (IR) The staining was semiquantitatively evaluated by assigning a score for the intensity of the IR and for the proportion of cells positively stained The intensity of IR (intensity...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
Ngày tải lên :
07/03/2014, 21:20
... distribution of VBARP is independent of the cycling stage of the cells Identification of biological function(s) of VBARP using a siRNA assay To examine the role of endogenous VBARP in the regulation of ... further confirming the specificity of this interaction Based on these results we were able to demonstrate the formation of a complex between Vpr and VBARP Characterization of VBARP variants The ... cell lines of different lineages for the presence of the above three isoforms (Fig 3B) Results indicated that VBARP-L is present in most of the tested cell lines with varying amounts with the highest...
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Báo cáo khoa học: Mitochondrial transcription factor A overexpression and base excision repair deficiency in the inner ear of rats with D-galactose-induced aging pdf
Ngày tải lên :
14/03/2014, 23:20
... harboring the common deletion By applying the specific probe, we can even detect the presence of the common deletion in the inner ear in the control group The proportions of the common deletion in the ... Zhong et al Increased mRNA level of TFAM induced by D-Gal Fig Effect of D-Gal on the amount of the mitochondrial common deletion in the inner ear The percentages of the mitochondrial common deletion ... protein level of TFAM induced by D-Gal To further understand the expression of TFAM protein in the inner ear, western blot analysis was performed The protein levels of TFAM in the inner ear of d-Galtreated...
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Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot
Ngày tải lên :
16/03/2014, 12:20
... indicated by the numbers on the right side The migration of the protein markers is indicated by pencil marks on the membrane The arrow indicates the position of the wild-type and N130D variants of human ... activity of the bacterial strain used to express the recombinant enzymes was excluded by the DEAE– cellulose chromatographic step of the purification protocol and the immunological properties of the ... charge introduced at position 130 The relevance of the negative charge at position 130 for the altered properties of the N130D variant was supported by the effects of introducing a glutamine residue...
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Báo cáo khoa học: Characterization of the dimerization process of a domain-swapped dimeric variant of human pancreatic ribonuclease pdf
Ngày tải lên :
16/03/2014, 13:20
... expression of the partial heat capacity of the protein relative to the heat capacity of the protein in the native state the monomerization of the dimer), and the latter process precludes the study of the ... residues of one subunit and Arg104 residues of the other [8] In this dimer, the relative positions of the two subunits would prepare the molecule for the swapping of the N-terminal domain while, in the ... corresponding to the Kd of DPM8 Thermal unfolding of monomeric PM8 Fig Kinetic analysis of dimerization of the human pancreatic ribonuclease variant, PM8 (A) Aliquots of the monomeric form of PM8 (MPM8)...
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Báo cáo khoa học: Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding ppt
Ngày tải lên :
16/03/2014, 19:20
... temperature dependence of the ellipticity at 220 nm in the range of 25–85 °C; the temperature was continuously changed at a rate of 0.5 °CÆmin)1 Tm (temperature at the midpoint of the thermal transition) ... family of proteins with a high degree of sequence identity (Fig 1C) Most of the differences among them involve the exposed regions, mainly the NH2-terminal b-hairpin that is the subject of this ... FEBS colonize the respiratory tract of the host leading to the pathological events [14] Considering the above arguments and the structural characteristics of the NH2terminal b-hairpin of ribotoxins,...
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Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx
Ngày tải lên :
16/03/2014, 23:20
... significantly in the presence or absence of the b1B subunit, whereas, under the same conditions, the rate of inactivation was increased in the presence of the b1 subunit The effects of b1B were further assessed ... that the intracellular segment of the b1 subunit is required for the interaction with the a subunit, probably a crucial step for the regulation of channel properties The tissue distribution of the ... from that of the rat b1A subunit Therefore, a RACE-PCR technique was applied to clone a novel, splicing variant of the human b1 subunit To perform RACE-PCR of a novel C terminus of the human...
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Báo cáo khoa học: Calcium-induced activation and truncation of promatrix metalloproteinase-9 linked to the core protein of chondroitin sulfate proteoglycans pot
Ngày tải lên :
17/03/2014, 10:20
... prevent the appearance of the released forms of MMP-9 from the complex The arrowhead shows the border between the stacking and separating gels, and the arrow shows the position of the 72 kDa form of ... removed from the sample, demonstrating that the CS-chains were not involved in the calcium -induced processing and release of the gelatinase from the complex The CaCl2 -induced conversion of the MMP-9/CSPG ... the gelatinase, as well as the cABC degradation of the CS-chains of the PG The presence of 4.5 M urea in the proMMP-9/CSPG preparation had no effect on the position of the two main gelatinase bands...
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