the main products derived from the blue protocol
Q5AR1 VIRAL SAFETY EVALUATION OF BIOTECHNOLOGY PRODUCTS DERIVED FROM CELL LINES OF HUMAN OR ANIMAL ORIGIN
Ngày tải lên :
04/12/2017, 04:16
... with BSE with the regulatory authorities The scope of the document covers products derived from cell cultures initiated from characterised cell banks It covers products derived from in vitro ... is the sum logarithm of the reduction factors of the individual steps It represents the logarithm of the ratio of the virus load at the beginning of the first process clearance step and at the ... to the specific treatment and it is used in the context of the understanding of the biology of the virus and the nature of the manufacturing process Actual results will vary according to the...
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Tài liệu Báo cáo khoa học: Different modes of dipeptidyl peptidase IV (CD26) inhibition by oligopeptides derived from the N-terminus of HIV-1 Tat indicate at least two inhibitor binding sites doc
Ngày tải lên :
20/02/2014, 11:20
... over 120 s The DP IV concentration in the assay was obtained from the graph of initial velocity vs the inhibitor concentration as the intersection of the regression line with the x-axis The DP IV ... for the Dixon plot where Ki is the binding constant of the inhibitor to the noncompetitive site on the enzyme, whilst a is the factor relating the difference in affinity of the inhibitor for the ... sites at the enzyme was reflected in the parabolic behavior of the Dixon plot (Fig 2A), which therefore was not suitable for the determination of the Ki value From the Hill-plot, binding of the inhibitor...
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Báo cáo khoa học: Study of synthetic peptides derived from the PKI55 ppt
Ngày tải lên :
07/03/2014, 05:20
... of the a, b1 and b2 isoforms [13] The present study aimed to identify peptides derived from the amino acid sequence of the PKI55 protein to be used as pharmacological tools The effects of the ... inhibition of the PKC a, b1, b2, c, d, e and f isoform enzyme activity in the presence of the PKI55 protein and the derived peptides 5, and 9, all tested at a concentration of lM The data are the mean ... bands (Fig 5) The lack of an effect on the a, b2 and f isoforms is also shown in Fig Discussion In the present study, selected peptides derived from the amino acid sequence of the PKI55 protein...
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PRINCIPLES FOR THE RISK ANALYSIS OF FOODS DERIVED FROM MODERN BIOTECHNOLOGY
Ngày tải lên :
13/03/2014, 21:56
... recognised that there are other applications of product tracing These applications should be consistent with the provisions of the SPS and TBT Agreements The application of product tracing to the areas ... knowledge and other information relevant to risk analysis 30 Recognizing the rapid pace of development in the field of biotechnology, the approach to safety assessments of foods derived from modern ... measures for foods derived from modern biotechnology should be proportional to the risk, based on the outcome of the risk assessment and, where relevant, taking into account other legitimate factors...
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Báo cáo khoa học: Small peptides derived from the Lys active fragment of the mung bean trypsin inhibitor are fully active against trypsin pptx
Ngày tải lên :
15/03/2014, 09:20
... the present article, we describe the cDNA cloning of MBTI, and the gene expression of a 35-residue peptide and its mutant both derived from the long chain of the Lys active fragment of MBTI The ... several BBIs from other leguminous dicotyledons (Fig 2) The MBTI gene (GeneBank accession number AY251011) was then amplified from total genomic DNA, demonstrating that there is no intron in the genomic ... letters The sequence of the signal peptide is shaded, and the following eight residues comprise the putative prosequence The sequence corresponding to the 80-residue mature protein is in bold The...
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc
Ngày tải lên :
17/03/2014, 10:20
... in the N-terminal domain [31] that binds the FAD-cofactor [17], one can speculate that the N-terminal domain of NifL might enter the lipid bilayer and contact the quinones dissolved within the ... measurement at 450 nm of the MBP-NifL reduction The open arrows indicate the time period during which the absorbance decreases due to NifL reduction by electrons derived from the inside-out vesicles ... (RAS48) and the nuoCD mutant strain of UN4495 (RAS47) carrying a chromosomal nifK¢¢lacZ fusion (A) Synthesis rates of b-galactosidase from the nifHDK promoter were determined from the slope of these...
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Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx
Ngày tải lên :
31/03/2014, 09:20
... °C The eluate containing enterokinase was added again to the column The operation was repeated three times which took about 10 The cleavage yield was eluted from the GSH gel in the water The ... cDNA The yield from the initial screening of the cDNA library with the 32P-labeled cDNA probe was about 260 positive clones On the final screening, 25 clones were selected on the basis of the strength ... corresponding to the mature toxin region as primers, and the BmKIM genomic DNA as template for PCR, the nucleotide sequence obtained was the same as the cDNA Therefore, there is only one intron in the sequence...
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báo cáo hóa học:" Comparative study on the immunogenicity between an HLA-A24-restricted cytotoxic T-cell epitope derived from survivin and that from its splice variant survivin-2B in oral cancer patients" pot
Ngày tải lên :
18/06/2014, 15:20
... splicing variants was shown only in the thymus [22] We then analyzed the survivin expression in the protein level In all the oral cancer cell lines examined in the present study, wild type survivin ... detected in all the cases by immunostaining Though there were some differences in the staining intensity among the cases, we couldn't find any correlation between the staining intensity and the CTL induction ... participated in the design of the study and performed the evaluation of the data TT helped to draft the manuscript SI contributed to the HLA-A24-binding assay and CTL induction from PBMCs AM, AY and...
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báo cáo hóa học: " Development and validation of a preference based measure derived from the Cambridge Pulmonary Hypertension Outcome Review (CAMPHOR) for use in cost utility analyses" doc
Ngày tải lên :
18/06/2014, 19:20
... six items These six items were combined into four domains such that two domains had three levels and the other two domains had two levels The items were selected by re-analysis of the responses ... indicating that the model is a good fit for the data For the random effects model, the results were similar in that all the coefficients had the expected negative sign but differed from the mean level ... is valued on the same scale using the same variant of the same valuation technique, as was the case for the CAMPHOR and EQ-5D models, then the valuations should be comparable [25] The valuation...
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Báo cáo hóa học: "Research Article A Dependent Multilabel Classification Method Derived from the k-Nearest Neighbor Rule" ppt
Ngày tải lên :
21/06/2014, 05:20
... allows us to rank the different labels according to their scores For an instance x, the higher the rank of a label ω, the larger the value of the corresponding score f (x, ω) Note that the multilabel ... Over the 11 subproblems, the number of categories varies from 21 to 40 and the instance dimensionality varies from 438 to 1.047 Table shows the statistics of the different subproblems within the ... evaluates the ranking quality of the different possible labels [6, 10] Let rank f (·, ·) be the ranking function derived from f and taking values in {1, , Q} For each instance xi , the label with the...
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Báo cáo toán học: "On the Modes of Polynomials Derived from Nondecreasing Sequences" pdf
Ngày tải lên :
08/08/2014, 12:23
... of the ratio monotonicity of the Boros-Moll polynomials [3] Let M∗ (P, d) and M ∗ (P, d) denote the smallest and the greatest mode of P (x + d) respectively Our main result is the following theorem, ... denote the n-th derivative of fk (x) Our proof of Theorem 1.1 relies on the fact that fk (x) has at most one real zero on (n) (0, +∞) In fact, the derivative fk (x) of order n ≤ m − k has the same ... establish this property by induction on n the electronic journal of combinatorics 18 (2011), #P1 2 Proof of Theorem 1.1 To prove Theorem 1.1, we need the following three lemmas Lemma 2.1 For any...
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Báo cáo y học: "Subtyping patients with heroin addiction at treatment entry: factor derived from the Self-Report Symptom Inventory (SCL-90)" ppt
Ngày tải lên :
08/08/2014, 23:21
... against them or exploit them with unpredictable consequences They think they are not respected by their workmates or are disapproved of because of their own views They get the impression that others ... others not sympathise with them or approve of their behaviour, or even show explicit hostility towards them They feel uneasy when they find other people staring at them or simply in speaking ... have moments when they cry or throw objects with the aim of breaking them or smashing them into pieces, and suffer from outbursts of rage They often get into arguments and feel the urge to push,...
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Báo cáo khoa học: "Branchiness of Norway spruce in northeastern France: predicting the main crown characteristics from usual tree measurements" doc
Ngày tải lên :
08/08/2014, 23:22
... measured: the diameter at breast height the nearest mm), the total height (to the nearest 10 cm), the age at the stump (to the nearest to yr, depending on age), the height to the first live branch, the ... increases from the top of the tree to a point that is near to the level of maximum lateral extension of the crown; and ii) that it then decreases towards the base of the tree The size of the branches ... scale of the annual shoot to the top of the stem (in m); RX= 100 (X/H) = relative distance from the upper bud scale scars of the annual shoot to the top of the stem (in %); NGU = No of the growth...
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Báo cáo y học: "Novel splice variants derived from the receptor tyrosine kinase superfamily are potential therapeutics for rheumatoid arthritis" pdf
Ngày tải lên :
09/08/2014, 10:23
... variants, derived from the extracellular domains of the 21 type receptor genes, were confirmed to be novel – with variants from the c-Met protooncogene being the most diverse (Table 1) Sequences of the ... activity in the particular disease model In vivo screening of the ASV demonstrated clear differential effects Among them, ASV derived from VEGFR1 and Tie1 were found to be the most potent The effect ... following the manufacturer's instructions For PCR amplification, gene-specific PCR primers were selected The forward primers flanked the start codon The reverse primers were selected from the transmembrane...
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Báo cáo sinh học: " The incidence of polyploidy and mixoploidy in early bovine embryos derived from in vitro fertilization" ppt
Ngày tải lên :
09/08/2014, 18:22
... Ovaries from randomly chosen slaughtered cows were collected at the local slaughterhouse within h (30-40 ovaries) and then transported to the laboratory in saline solution at 30-37 °C They were then ... blastomere from a diploid line was analysed The triploidy and tetraploidy observed in the present study were diagnosed mainly on the basis of a single metaphase so it is possible that these embryos ... (Merck, Germany) The oocytes were then incubated in the droplets at 39 °C in The COCs ECS + 10 were humid 5% C0 Sperm atmosphere for 26 h treatment Frozen sperm was processed according to the method...
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Báo cáo y học: "A vertebrate case study of the quality of assemblies derived from next-generation sequence" doc
Ngày tải lên :
09/08/2014, 22:24
... total, 81.0% of the novel sequences from the Illumina/SOAP assembly and 48.1% from the 454/Newbler assembly matched recently sequenced BACs We found 16.3% of the novel sequences from the Illumina/SOAP ... Mbp), derived from the same DNA source as the reference, the reference assembly covered 98.4% of total bases, while the NGS assemblies covered 96.0% (454/Newbler) and 95.6% (Illumina/SOAP) of the ... if the percentage identity (95%) and length of the mapped portion was over the indicated cutoff The alignment length cutoffs shown are 90%, 50%, and 20% Blue represents the reference, red the...
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báo cáo khoa học: " Gene-based SSR markers for common bean (Phaseolus vulgaris L.) derived from root and leaf tissue ESTs: an integration of the BMc series" docx
Ngày tải lên :
11/08/2014, 11:21
... represented the main axis of the neighbor joining tree upon which two of the wild accessions then showed a divergence from the cultivated genotypes The Argentinean accession G19892 grouped within the ... Across all the EST sequencing sets the percentage of ESTs containing SSRs varied from 3.5 to 11.9% with the highest number found in the first sequencing of the leaf library and the least in the second ... sequenced from the DOR364 root tissue libraries: these being 862 from the HP library (GenBank entries, HS103978-HS104836) and 953 from the LP library (GenBank entries, HS103028-HS103977) Clones from...
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Báo cáo y học: " Peptides derived from the HIV-1 integrase promote HIV-1 infection and multi-integration of viral cDNA in LEDGF/p75-knockdown cells" doc
Ngày tải lên :
12/08/2014, 04:20
... complex The complex was then incubated with either the indicated peptide or BSA at the designated IN:peptide (or BSA) ratios The amount of bound LEDGF/p75 was then determined (C) Same as (B) but the ... stimulate the enzymatic activity of the IN itself in vitro, and consequently the integration of viral cDNA in virus infected cells [25] In the current work we show that in the presence of the INr ... cells Furthermore, a relatively high degree of viral cDNA integration was observed in these cells following their incubation with the INr and INS peptides These results indicate that the previously...
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Báo cáo y học: " A 176 amino acid polypeptide derived from the mumps virus HN ectodomain shows immunological and biological properties similar to the HN protein" pdf
Ngày tải lên :
12/08/2014, 04:20
... defined on the basis of the nucleotide sequence of the MuV SH gene [6,10,21] Furthermore, two important mumps outbreaks were recently reported, one in 2005 in the UK, and the other in 2006 in the USA ... compared to the activity in MuV-infected cells Because the main receptor binding domain of the MuV HN protein has been proposed to be located at the same site as the neuraminidase activity [34], the ... Furthermore, the sera neutralized both MuV strains to the same extent, even though these strains belonged to a different MuV genotype The first one was the B genotype, and the last one was the...
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Báo cáo y học: "Characterization of monocyte/macrophage subsets in the skin and peripheral blood derived from patients with systemic sclerosis" pps
Ngày tải lên :
12/08/2014, 14:22
... the ordinate *P < 0.05 as compared with the value in cells from healthy controls (c) The left panel shows the representative pattern of CD204 histogram The number of peaks was counted as in the ... Taken together, the presence of the CD14brightCD163+CD204+ monocyte subset may be characteristic in SSc PBMCs Discussion Our study revealed three major findings First, in the skin from SSc patients, ... depicts the summary of results, comparing percentages of CD14 positive cells in PBMCs (shown on the ordinate) from healthy controls and SSc patients *P < 0.05 as compared with the value in cells from...
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