... byEcR in the presence of ligand was maximum in the case of HsRXR and minimum in the case of the Hs–LmRXR chimera, and LmRXR was betweenthese two (Fig. 9). These data suggest that the EcRand ... beenused in the place of USP in all EcR gene switchesdeveloped to date. One ofthe major limitations of cur-rent versions of EcR gene switches is the requirement of micromolar concentrations of ligand ... concentrations of ligandsfor the transactivation of genes and functions wellin vivo. The RXR chimeras containing most of HsRXR and helix 9 and the loops on either side of helix 9 from HsRXR or mutants of...
... critically dependon the other free parameters ofthe model, a property of the used function. On the other hand, this meansthat the other parameters following from the fit of the data cannot be ... to the inherentexperimental errors of our assay. However, the expres-sion of CAT under theinfluenceof any ofthe threeused CSPs resulted in a significant, dramatic decrease of protein synthesis ... terms of codon usage and minimization of possible RNA secondary structures. We verified the difference in the thermodynamics ofthe RNA secon-dary structures by predicting the free energy of the RNA...
... mayreach the point where all ofthe membrane is homoge-neous. The MPRP-C is insensitive to the cholesterolcontent in the membrane, which is in agreement with the insensitivity ofthe peptide to the ... about the interaction of peptides with large unilamellar vesicles(LUV). The fluorescence quantum yield is dependent on the polarity ofthe microenvironment ofthe Trp resi-dues, as well as on the ... The membrane interaction behaviour of MPRP-Cand MPRP-I is independent ofthe membrane phasebut not ofthe presence of charged lipids. Cholesteroldoes not reduce the extent of or potentiate...
... [15]. The second electron rereduces CuAthereby forming the initial two-electron reduced oxidase. In the absence of oxygen or the presence of CO, there is probably a two-electron reduction ofthe ... other factors. None-theless, catalytic activity is still one ofthe few characteristicsthat reflect the actual role ofthe oxidase in the tissues.What is that role? The oxidase catalyzes the ... increase the viscosity ofthe medium; ifthere are large conformational changes that occur in the oxidase during the catalytic cycle, these would be influencedby the increased viscosity. Certainly, there...
... with the adjacent pulse, and the correlation should6 EURASIP Journal on Advances in Signal Processingbe lower. The evaluation oftheinfluenceof jitter (as well of other levels of noise and their ... shimmer increases. On the other hand, the summation of tails in one pulse isnot completely uncorrelated with the summation of tails in the other. For this reason, the estimation of relative pulseamplitudes, ... used in the synthesis. The results for the different methods facing both noisetypes are shown in Figure 1, and the discussion below isfirst centered in the AWGN and later in the effect of the correlation...
... concerning theinfluenceof glycosylation on the protein biophysical properties but also support the hypothesis that fora-CT the global structural dynamics directly influencethe kinetics of enzyme ... some of these phenomena maybe interconnected within the catalytic mechanism of the enzyme.Structural insights into the mechanochemicalnature of a-CT catalysisA more detailed analysis oftheinfluence ... within a cutoff distance (rc¼ 6.0 A˚). The dynamics of the resulting network are then defined by the Ni · Nj Kir-chhoff connectivity matrix of interresidue contacts (G)where the off diagonal...
... the similar bio-chemical and thermostability properties of theseenzymes [7]. Comparisons between the structures of the most thermostable class I XIs from Thermus caldo-philus and Thermus thermophilus ... presence of various metal concen-trations and metal combinations was determined.Figure 7 shows the inactivation courses of apo-TNXIand of TNXI in the presence of 5 mm concentrations of each ofthe ... hyperthermophiles are intrinsically ther-mostable and thermoactive, with optimal temperatures of activity often in excess of 100 °C [1]. Studies focus-ing on the molecular basis for the thermostability...
... lowers the intracellular levels of benzoate and fluoresceinby catalysing an active efflux of these compounds from the cell [2,4]. The inhibitory effects of different organic acids oncells ofthe ... from the Euroscarf collec-tion that lack specific enzymes ofthe aromatic amino acidbiosynthetic pathway. These were then tested for theirsensitivities to both acetate and sorbate. Growth of the wild-type ... several ofthe enzymes of aromaticamino acid biosynthesis therefore hypersensitize yeast toweak organic acid stress.We also investigated the growth of this same series of strains after they had...
... wellas changes in the aerosol and oxidant composition. Theinfluenceof changes in the 15aerosol composition are reflected in the changes ofthe microphysical aging time of BCand POM. The microphysical ... biomassburning.15 The emissions are detailed for the different sectors in Table 1. The evolution of the aerosol emissions for the different experiments are discussed together with the resulting changes in the ... among the aerosol modes, condensation of gas-phasesulfuric acid onto the aerosol surface, the formation of new particles by binary nucle-ation of sulfate, and the water uptake depending on the thermodynamic...
... either from the intensity ⁄ temperature plot ofthe b-sheet band of b2m at 1592 cm)1or from the frequency ⁄ temperature changes of the tyrosine ring vibration ofthe HC at 1514 cm)1 of the ... are due to different packing ofthe protein chainsin crystallo. A comparison ofthe X-ray structuresrevealed, however, that the structures ofthe HC and of b2m ofthe two subtypes appear nearly ... irrespective ofthe sequence ofthe bound peptide and itsbinding mode. They might thus influence antigenic properties ofthe respec-tive HLA-B27 subtype. Furthermore, a decrease in the pH from...