the influence of discourse

... by EcR in the presence of ligand was maximum in the case of HsRXR and minimum in the case of the Hs–LmRXR chimera, and LmRXR was between these two (Fig. 9). These data suggest that the EcR and ... been used in the place of USP in all EcR gene switches developed to date. One of the major limitations of cur- rent versions of EcR gene switches is the requirement of micromolar concentrations of ligand ... concentrations of ligands for the transactivation of genes and functions well in vivo. The RXR chimeras containing most of HsRXR and helix 9 and the loops on either side of helix 9 from HsRXR or mutants of...

Ngày tải lên: 07/03/2014, 12:20

... critically depend on the other free parameters of the model, a property of the used function. On the other hand, this means that the other parameters following from the fit of the data cannot be ... to the inherent experimental errors of our assay. However, the expres- sion of CAT under the influence of any of the three used CSPs resulted in a significant, dramatic decrease of protein synthesis ... terms of codon usage and minimization of possible RNA secondary structures. We verified the difference in the thermodynamics of the RNA secon- dary structures by predicting the free energy of the RNA...

Ngày tải lên: 07/03/2014, 21:20

... may reach the point where all of the membrane is homoge- neous. The MPRP-C is insensitive to the cholesterol content in the membrane, which is in agreement with the insensitivity of the peptide to the ... about the interaction of peptides with large unilamellar vesicles (LUV). The fluorescence quantum yield is dependent on the polarity of the microenvironment of the Trp resi- dues, as well as on the ... The membrane interaction behaviour of MPRP-C and MPRP-I is independent of the membrane phase but not of the presence of charged lipids. Cholesterol does not reduce the extent of or potentiate...

Ngày tải lên: 23/03/2014, 07:20

... [15]. The second electron rereduces Cu A thereby forming the initial two-electron reduced oxidase. In the absence of oxygen or the presence of CO, there is probably a two- electron reduction of the ... other factors. None- theless, catalytic activity is still one of the few characteristics that reflect the actual role of the oxidase in the tissues. What is that role? The oxidase catalyzes the ... increase the viscosity of the medium; if there are large conformational changes that occur in the oxidase during the catalytic cycle, these would be influenced by the increased viscosity. Certainly, there...

Ngày tải lên: 31/03/2014, 07:20

... with the adjacent pulse, and the correlation should 6 EURASIP Journal on Advances in Signal Processing be lower. The evaluation of the influence of jitter (as well of other levels of noise and their ... shimmer increases. On the other hand, the summation of tails in one pulse is not completely uncorrelated with the summation of tails in the other. For this reason, the estimation of relative pulse amplitudes, ... used in the synthesis. The results for the different methods facing both noise types are shown in Figure 1, and the discussion below is first centered in the AWGN and later in the effect of the correlation...

Ngày tải lên: 21/06/2014, 20:20

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Ngày tải lên: 22/06/2014, 18:20

... concerning the influence of glycosylation on the protein biophysical properties but also support the hypothesis that for a-CT the global structural dynamics directly influence the kinetics of enzyme ... some of these phenomena may be interconnected within the catalytic mechanism of the enzyme. Structural insights into the mechanochemical nature of a-CT catalysis A more detailed analysis of the influence ... within a cutoff distance (r c ¼ 6.0 A ˚ ). The dynamics of the resulting network are then defined by the Ni · Nj Kir- chhoff connectivity matrix of interresidue contacts (G) where the off diagonal...

Ngày tải lên: 19/02/2014, 05:20

... the similar bio- chemical and thermostability properties of these enzymes [7]. Comparisons between the structures of the most thermostable class I XIs from Thermus caldo- philus and Thermus thermophilus ... presence of various metal concen- trations and metal combinations was determined. Figure 7 shows the inactivation courses of apo-TNXI and of TNXI in the presence of 5 mm concentrations of each of the ... hyperthermophiles are intrinsically ther- mostable and thermoactive, with optimal temperatures of activity often in excess of 100 °C [1]. Studies focus- ing on the molecular basis for the thermostability...

Ngày tải lên: 23/03/2014, 13:20

... lowers the intracellular levels of benzoate and fluorescein by catalysing an active efflux of these compounds from the cell [2,4]. The inhibitory effects of different organic acids on cells of the ... from the Euroscarf collec- tion that lack specific enzymes of the aromatic amino acid biosynthetic pathway. These were then tested for their sensitivities to both acetate and sorbate. Growth of the wild-type ... several of the enzymes of aromatic amino acid biosynthesis therefore hypersensitize yeast to weak organic acid stress. We also investigated the growth of this same series of strains after they had...

Ngày tải lên: 23/03/2014, 21:20

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Ngày tải lên: 05/07/2014, 21:20

Ngày tải lên: 05/07/2014, 21:20

Ngày tải lên: 18/09/2013, 21:26

... well as changes in the aerosol and oxidant composition. The influence of changes in the 15 aerosol composition are reflected in the changes of the microphysical aging time of BC and POM. The microphysical ... biomass burning. 15 The emissions are detailed for the different sectors in Table 1. The evolution of the aerosol emissions for the different experiments are discussed together with the resulting changes in the ... among the aerosol modes, condensation of gas-phase sulfuric acid onto the aerosol surface, the formation of new particles by binary nucle- ation of sulfate, and the water uptake depending on the thermodynamic...

Ngày tải lên: 17/02/2014, 10:20

... either from the intensity ⁄ temperature plot of the b-sheet band of b 2 m at 1592 cm )1 or from the frequency ⁄ temperature changes of the tyrosine ring vibration of the HC at 1514 cm )1 of the ... are due to different packing of the protein chains in crystallo. A comparison of the X-ray structures revealed, however, that the structures of the HC and of b 2 m of the two subtypes appear nearly ... irrespective of the sequence of the bound peptide and its binding mode. They might thus influence antigenic properties of the respec- tive HLA-B27 subtype. Furthermore, a decrease in the pH from...

Ngày tải lên: 06/03/2014, 00:21