... CXCL10 AST/Mat AST IL-12 p35 IL-12 p40 0.02 Mat Control AST/Mat AST Mat 0.00 Control AST/Mat AST Mat Control 0.05 1.0 0.8 0.015 0.01 0.010 AST/Mat AST 0.000 Mat AST/Mat AST Mat 0.00 Control AST/Mat ... using the highly sensitive Limulus assay indicated only trace reactivity for the purified human material and none for the recombinant peptide Matrigel angiogenesis assay The assay was performed as ... Folkman J: Inhibition of plaque neovascularization reduces macrophage accumulation and progression of advanced atherosclerosis Proc Natl Acad Sci USA 2003, 100:4736-4741 Chavakis T, Athanasopoulos...
... possible to investigate human language equally systematically and on an equally grand scale: aHuman Linguome Project, as it were, though we have chosen theHuman Language Project” asa more inviting ... purposes a perfectly adequate meaning representation That is, we view machine translation as an approximation to language understanding Here is another way to put it One measure of adequacy ofa language ... can turn the size ofthe task—constructing MT systems for all the world’s languages simultaneously—to one’s advantage, and thereby consume dramatically less data per language One ofthe greatest...
... were dried and autoradiographed Competitor DNAs used in EMSA analysis were: NF1 wt, 5¢-TTTTG GATTGAAGCCAATATGATA-3¢; NF1 mut, 5¢-TTTT GGATTGAATAAAATATGATA-3¢; Site-2 wt, 5¢-GCGT CTCACCCTAGTCCTGGTCCTGCTCCAAGGGTTTT ... activities were measured as described [17] DNase I protection assay Rat liver and HeLa nuclear extracts were prepared as described previously [18,19] The DNase I protection assay was performed as described ... binding activity in all fractions was monitored by the in vitro DNase I protection assay The DNA affinity column, used asthe last step in the purification, was prepared with an oligonucleotide containing...
... RT-PCR using PlatiniumÒ Taq DNA High Fidelity Polymerase (Invitrogen) and the primers: PDZ-1-2, forward: 5¢-CCGAATTCGAAGAAATCACACTTGAAAGG-3¢, and reverse: 5¢GGATCCCCATCATTCATATACATACTTGT GGGTT-3¢; ... mutagenic primers were: GRRF-PDZ1: 5¢GAAAGGGGAAATTCAGGGCGTCGT TTCAGCATTGCAGGAGG-3¢; GRRF-PDZ2: 5¢-ATTA AAGGTCCTAAAGGTCGTCGGTTTAGCATTGCTGGA GG-3¢; RAAV-MEK2: 5¢-CACCCACGCGCGCCGCCGT GTGA-3¢ and ... least one MAPK implicated in ERK activation to facilitate a functional interaction and regulate the localization and the duration ofthe signal For example, the MEK partner directs the ERK cascade...
... obtained was used asa template for real-time quantitative polymerase chain reaction, which was performed with the LC FastStart DNA Master SYBR GreenI® (Roche Diagnostics, Mannheim, Germany) in a LightCycler® ... 2:116-126 20 Matsuyama T, Yamada A, Kay J, Yamada KM, Akiyama SK, Schlossman SF, Morimoto C: Activation of CD4 cells by fibronectin and anti-CD3 antibody A synergistic effect mediated by the VLA-5 fibronectin ... receptors and are activated by lipopolysaccharide J Exp Med 2003, 197:403-411 Nagai Y, Akashi S, Nagafuku M, Ogata M, Iwakura Y, Akira S, Kitamura T, Kosugi A, Kimoto M, Miyake K: Essential role of...
... (CDD) databases (Figure 2a; Additional data file 2) Components of canonical signaling pathways were included (for example, Ca2+, cAMP, and ERK), as well as less characterized and putative signaling ... d-siRNAs that resulted in an absolute value ofthe CAsH score ≥0.95 (see Additional data file for the list of primary hits and Figure S4 in Additional data file for the distribution ofthe CAsH ... signaling pathway (b) Quantification ofthe effects of different siRNAs targeting the PtdIns(3,4,5)P3-mTOR pathway and rapamycin (RAPA) on transferrin uptake Means ± standard error ofthe mean...
... used to analyse the associations between the risk factors and the outcome variable The analysis was performed in three stages: initially, analysis was performed to establish the association between ... Additional analysis treating days of sickness absence during 1990 asa continuous variable showed a clear trend of increase in disability pension risk with increase in absence days/yr A 10-day increase ... Ministry of Employment, the Ministry of Social Affairs and the Ministry of Education DWECS was conducted in 1990, and featured a random sample drawn from the Central Population Register of Denmark of...
... display the hallmarks ofthe school I associate them with The hallmarks ofthe natural school are that humanrights are conceived as entitlements and that humanrightslaw is regarded favourably ... by means ofthelawas well as by means ofa wider culture that sustains and motivates thelaw .133 As far as he is concerned, it cannot be assumed that ‘universal capital or universal human right ... progress ofhumanrights since 1948’112 chimes with the fact that natural scholars conceive ofhumanrightslawasa direct continuation ofhumanrights It has been said that the lawyers are ‘the...
... with the right job-related knowledge, skills and educational level, as well asthe adoption of performance appraisal There is greater emphasis on management according to law and accountability has ... while offering them legal protection against arbitrary and incompetent officials and the mass media The cost ofthe civil service as streamlined and measures were taken to decrease the use of indirect ... professional standards and norms and encouraging a sense of accountability and transparency in activities related to citizens Another aim was to reform the salary system to ensure that the salaries of...
... (1) The ratio ofthe epidural and cesarean components ofthe OAAI (OAAI EPI and OAAI CD) was also calculated as follows: OAAICD/EPI = ( no of cesareans per yr *1.5 ) / ( no of epidurals per ... patient-controlled analgesia pumps The OAAI ignores clinical activities other than epidural analgesia and cesarean anesthesia (including anesthesia for retained placenta and complicated vaginal deliveries, antenatal ... allocated for the provision of obstetric anesthesia services The OAAI was calculated based on the premise that epidurals and cesareans are the predominant determinants of obstetric anesthesia...
... 5¢-GCAGCAUCUUUAAUGAAUAdTdT-3¢ and 5¢-AUAAGUAAUUUCUACGACG dTdT-3¢; Nup358, 5¢-CCAGUCACUUACAAUUAAAd TdT-3¢ and 5¢-UUUAAUUGUAAGUGACUGGdTdT-3¢ (siNup358-1), 5¢-UGAAGCACAUGCUAUAAAAdTdT-3¢ and 5¢-UUUUAUAGCAUGUGCUUCAdTdT-3¢ ... CCACCGCTTGAGAGACTTACTCTTGATTGTAACGA GGATA-3¢ and 5¢-AGCTTATCCTCGTTACAATCAA GAGTAAGTCTCTCAAGCGGTGGTAGCTGAAGAGG A- 3¢) were annealed and inserted into the BglII and HindIII sites of pEGFP-NLS The oligonucleotide ... GDP, across the nuclear envelope regulates the binding and release of cargo by transport factors RanGTP is abundant in the nucleus asa result ofthe activity of RCC1, a guanine nucleotide exchange...
... release of ROS from NADPH oxidase and mitochondria, sustained increase ofthe cytosolic Ca2+ concentration and finally nuclear translocation of mitogen-activated protein kinase kinase ⁄ extracellular ... currently available PARP-1 activation and cell death in the neurovascular unit Poly(ADP-ribose) polymerases (PARPs) are NADdependent enzymes that are able to catalyse the transfer of ADP-ribose ... histological basis Also, careful and rigorous selection of patients with salvageable tissue [evidenced using magnetic resonance imaging asthe presence of an area of hypoperfusion larger than that of altered...
... [28–30] Asa means of quality assurance, we analyzed the proteins by liquid chromatography-coupled (LC-MS) and native MS The measured masses obtained by LC-MS are depicted in Table Using the denaturing ... results Stoichiometry (native ESI-MS) Average mass calculated (Da)b Mass measured LC-ESI-MS Mass measured ESI-MS (native) (Da) a W15-peptide TQAKRKKSLAMA [30] ine c As previously measured [31] b R77L ... times (1 h), revealed no major other cleavage products (data not shown) Addition of cGMP had a remarkable effect on the stability ofthe R77L mutant Now, a rather rapid degradation was observed (Fig...
... the influence ofhuman cyt b5 changes dramatically asthe cyt b5/P450c17 ratio varies Effect of P450red on DHEA and androstadienol synthesis To further investigate the modulation ofhuman P450c17 ... enzymatic assays using human and porcine P450c17 in the presence of various substrates – preg, 17aOHpreg and DHEA – and analyzed androstadienol formation from each substrate As observed in Fig 3, the ... biosynthesis of androstadienol in humans (A) and pigs (B) does not require prior formation of 1 7a- OH-preg and DHEA The lack of androstadienol synthesis in the presence of lM of ketoconazole (C), an...
... Thanks in West Kalimantan to Shaban Setiawan and all the staff at WALHI KalBar, Vincentius V., AMA (Aliansi Masyarakat Adat) KalBar, Adrianus Amit, Pemberdayaan Otonomi Rakyat (POR), John Bamba ... staff at Yayasan Elang, WALHI Riau, Kelompok Advokasi Riau, Aliansi Masyarakat Adat Riau (AMAR), Hakiki, Santo Kurniawan at Jikalahari Thanks to all the numerous other individuals and organisations ... p.IV-34, Laporan Akhir, Keterpaduan Kebijakan Antar Sektor dalam Peningkatan Daya Saing Kawasan Andalan dan Percepatan Pembangunan Daerah Tertinggal, Direktorat Kewilayahan II Kementerian Negara Perencanaan...
... In all cases, the optimal amount of arachidonic acid was determined and used to analyze the effect of S10 0A8 /A9 on oxidase activation After an incubation of 10 at 20 °C, the oxidase activity was ... an optimal amount of arachidonic acid determined for each assay of oxidase activation [12] The rate of O2– production by the activated NADPH oxidase was calculated from the rate ofthe superoxide ... NADPH oxidase activity was also assayed by polarographic measurement ofthe rate of O2 uptake at 20 °C with a Clark electrode at a voltage of 0.8 V All experiments were carried out at least twice...
... its head, with the property that the functional features of each phrase are identified with those of its head The head category ofa phrase is characterized by d~e assignment ofthe trivial ft~%ctional-equation ... to the fact that using LFG as syntactic base for IRT - and not the categorial syntax of Kamp - the ~raveling ofthe thematic relations in a sentence is already accomplished in f-structure Therefore ... relations ofa sentence But what about the logical relations? Recall that each clause has a unique head end that the functional features of each phrase are identified with those of its head For...
... tissue samples easily assessable in human subjects Abbreviations GCP: glutamate carboxypeptidase; NAAG: N-acetyl-aspartyl-glutamate; NAA: N-acetyl-aspartate; CSF: cerebrospinal fluid; CNS: central ... Page of Chromatographic analysis was performed using a Waters ACQUITY UPLC (Milford, MA, USA) Separation ofthe analytes from potentially interfering material was achieved at ambient temperature ... GLAST, EAAC1 and of GCPII in the rat peripheral nervous system J Anat 2008, 213:539-546 Cassidy M, Neale JH: N-acetylaspartylglutamate catabolism is achieved by an enzyme on the cell surface of...
... tissue samples easily assessable in human subjects Abbreviations GCP: glutamate carboxypeptidase; NAAG: N-acetyl-aspartyl-glutamate; NAA: N-acetyl-aspartate; CSF: cerebrospinal fluid; CNS: central ... Page of Chromatographic analysis was performed using a Waters ACQUITY UPLC (Milford, MA, USA) Separation ofthe analytes from potentially interfering material was achieved at ambient temperature ... GLAST, EAAC1 and of GCPII in the rat peripheral nervous system J Anat 2008, 213:539-546 Cassidy M, Neale JH: N-acetylaspartylglutamate catabolism is achieved by an enzyme on the cell surface of...
... in the car codebook and in airplane codebook have a similar appearance At the same time, each category also has a contextually related background codebook Therefore, each category has a category-specific ... Visual Context in Human Visual System 2.1 Part-Part Context According to Gestalt’s law, thehuman visual system actively utilizes the laws of proximity and similarity to discriminate the figural ... and appearance parameter for bottom-up inference The learning process is quite simple As shown in Figure 20, initially we have a set of CCBs, such asa car, or an airplane The appearance parameter...